##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551315_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	811768
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.631287510717346	31.0	31.0	34.0	30.0	34.0
2	31.77313592060786	31.0	31.0	34.0	30.0	34.0
3	31.72406278641188	33.0	31.0	34.0	30.0	34.0
4	35.431491509889526	37.0	35.0	37.0	33.0	37.0
5	33.45832429955357	37.0	35.0	37.0	30.0	37.0
6	34.333685240117866	37.0	35.0	37.0	30.0	37.0
7	35.39982236303969	37.0	35.0	37.0	32.0	37.0
8	35.24048003863173	37.0	35.0	37.0	32.0	37.0
9	37.022009983147896	39.0	37.0	39.0	33.0	39.0
10	36.60735579623735	38.0	35.0	39.0	32.0	39.0
11	36.728297986616866	39.0	35.0	39.0	33.0	39.0
12	36.696193986459186	39.0	35.0	39.0	33.0	39.0
13	36.793546924737115	39.0	35.0	39.0	33.0	39.0
14	37.79589488622365	40.0	37.0	41.0	33.0	41.0
15	37.881665697588474	40.0	37.0	41.0	33.0	41.0
16	37.986386504518535	40.0	37.0	41.0	33.0	41.0
17	37.807485636290174	40.0	36.0	41.0	33.0	41.0
18	37.79415916862946	39.0	36.0	41.0	33.0	41.0
19	37.70452395265642	39.0	36.0	41.0	33.0	41.0
20	37.76247770298903	40.0	36.0	41.0	33.0	41.0
21	37.70069650441998	39.0	36.0	41.0	33.0	41.0
22	37.80417311350041	40.0	36.0	41.0	33.0	41.0
23	37.85978506174178	40.0	36.0	41.0	33.0	41.0
24	37.739509317933205	40.0	36.0	41.0	33.0	41.0
25	37.487506282583205	39.0	35.0	41.0	33.0	41.0
26	37.53124784421165	39.0	36.0	41.0	33.0	41.0
27	37.13232844852224	39.0	35.0	41.0	32.0	41.0
28	37.17256900986489	39.0	35.0	41.0	32.0	41.0
29	37.432645780567846	40.0	35.0	41.0	32.0	41.0
30	37.16114456347134	39.0	35.0	41.0	32.0	41.0
31	37.214186811995546	39.0	35.0	41.0	32.0	41.0
32	37.204985907303566	39.0	35.0	41.0	32.0	41.0
33	37.156920450177886	40.0	35.0	41.0	32.0	41.0
34	37.03707709591903	40.0	35.0	41.0	32.0	41.0
35	37.00764873707759	40.0	35.0	41.0	32.0	41.0
36	36.788275714243476	39.0	35.0	41.0	31.0	41.0
37	36.78906658059938	39.0	35.0	41.0	31.0	41.0
38	36.641521961939866	39.0	35.0	41.0	31.0	41.0
39	36.53373377615279	39.0	35.0	41.0	31.0	41.0
40	36.431963319569135	39.0	35.0	41.0	30.0	41.0
41	36.30537296370392	39.0	35.0	40.0	30.0	41.0
42	36.452647800849505	39.0	35.0	41.0	31.0	41.0
43	36.39810265987327	39.0	35.0	41.0	31.0	41.0
44	36.52052926451893	39.0	35.0	41.0	31.0	41.0
45	36.50332730533847	39.0	35.0	41.0	31.0	41.0
46	36.38952262222704	39.0	35.0	41.0	31.0	41.0
47	36.27186708517704	39.0	35.0	41.0	31.0	41.0
48	36.25335687043589	39.0	35.0	41.0	31.0	41.0
49	36.224054163258465	39.0	35.0	41.0	31.0	41.0
50	36.11679322170867	39.0	35.0	40.0	30.0	41.0
51	35.20375033260735	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	22.0
9	32.0
10	51.0
11	35.0
12	42.0
13	41.0
14	58.0
15	71.0
16	104.0
17	151.0
18	252.0
19	543.0
20	1172.0
21	2123.0
22	3369.0
23	4343.0
24	5255.0
25	6045.0
26	7006.0
27	7874.0
28	8449.0
29	9761.0
30	12318.0
31	16099.0
32	21867.0
33	31544.0
34	55562.0
35	64300.0
36	68093.0
37	101263.0
38	168668.0
39	215187.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.3301534428556	20.018897024765696	23.24543465620719	12.405514876171516
2	31.716451005706066	28.561978299218495	23.326985049915738	16.3945856451597
3	31.366474165032376	27.092839333405607	26.09735786579417	15.443328635767855
4	28.10261059810192	23.557223246050597	30.24386277852786	18.09630337731963
5	27.37740339604419	31.8094578746637	23.07469621862404	17.738442510668072
6	25.337904425895086	36.23412107892896	24.124133989021495	14.303840506154467
7	75.10803579347794	6.132909895438106	14.173138137990165	4.58591617309379
8	75.4628169624819	9.827438381409467	9.771018320505366	4.938726335603276
9	69.55768150506057	7.812084240817574	11.618220969538093	11.012013284583773
10	42.9588256743306	24.219481428191305	18.527337860078248	14.294355037399848
11	32.62311399316061	23.846222073301732	24.79193562692789	18.73872830660977
12	29.199598900178376	20.905234007746056	28.15804515575879	21.73712193631678
13	26.880956135250468	22.582190970819248	30.65728139074218	19.879571503188103
14	20.198381803668042	28.781868711257403	28.104581604596383	22.915167880478165
15	19.06111105636093	25.59807728316465	34.02314454376127	21.31766711671315
16	22.387923643208403	24.369154733864846	29.76453863665481	23.47838298627194
17	21.765208778862927	26.711449576726352	28.92390436676489	22.59943727764583
18	22.461836386750893	25.34073774773088	28.932404332272277	23.265021533245953
19	24.417690768791083	26.219190704733368	26.268589055000934	23.09452947147461
20	26.980615151126923	25.848887859585496	29.312931773610195	17.857565215677383
21	24.81497176533197	27.759409092252955	28.15607414926432	19.269544993150753
22	21.957135536261593	24.312488297148942	29.709720018527463	24.02065614806201
23	24.335524435553015	25.696011668358448	28.496072769559778	21.472391126528763
24	23.3347458879877	25.41292586059071	28.411196302391815	22.841131949029773
25	22.751081589813836	28.723231268047027	26.34225542273162	22.183431719407515
26	21.3814784519715	26.49451567442915	29.437597934385195	22.68640793921416
27	24.455386267997753	26.690630820628552	27.36717879985415	21.486804111519547
28	20.02283903775463	27.94037212602615	29.915813385105107	22.120975451114113
29	23.511397345054252	23.750874634131918	30.856845798306903	21.880882222506923
30	23.313680756078092	27.07941185166205	28.32434882872939	21.28255856353047
31	23.85188871697332	25.669526268589056	25.50075883750037	24.977826176937253
32	27.294744311182505	25.845315410314278	26.539602447990067	20.320337830513154
33	24.687349094815268	25.065289590129197	26.79312315834081	23.454238156714727
34	23.156616176050303	23.978402696336882	29.33510559667294	23.529875530939876
35	24.519197603256103	22.97134156557046	29.30307674113786	23.206384090035577
36	22.66534280730455	26.707753939549228	28.2325738388308	22.39432941431542
37	24.797848646411293	25.560628159769788	28.362044327936058	21.279478865882865
38	22.580096776418877	24.713095367149236	27.893067970158963	24.813739886272927
39	25.803924273930484	23.78561362359689	28.530565383212934	21.87989671925969
40	23.568802909205587	22.304525430911294	31.727168353519726	22.399503306363393
41	21.01154517054134	24.458835529363068	30.022370923712195	24.5072483763834
42	21.727143715938542	24.06943855880005	31.142395364192726	23.06102236106868
43	23.19135516551527	24.426683485922084	28.96492593943097	23.417035409131675
44	23.55623774280336	23.72771530782194	28.590804269200063	24.12524268017463
45	22.810581348365545	22.452350917996274	28.24513900523302	26.491928728405163
46	24.211843778025248	25.750214346956273	27.629199475712273	22.408742399306206
47	21.20248642469277	25.278897418966995	31.903819810586278	21.61479634575396
48	22.11444649210119	22.906051975441258	30.109341585280525	24.870159947177026
49	22.020946871519943	23.015073272166433	31.966399266785583	22.997580589528045
50	21.776295690394303	22.877472381271495	30.14161681662741	25.20461511170679
51	21.0401247647111	22.8656463423047	29.059657439071263	27.034571453912942
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	296.0
1	870.0
2	1444.0
3	3675.0
4	5906.0
5	4069.0
6	2232.0
7	2145.0
8	2058.0
9	2046.0
10	2034.0
11	2043.5
12	2053.0
13	2017.0
14	1981.0
15	1965.0
16	1949.0
17	2077.5
18	2206.0
19	2027.5
20	1849.0
21	2307.5
22	2766.0
23	3063.5
24	3361.0
25	4162.5
26	6127.0
27	7290.0
28	8498.0
29	9706.0
30	11485.0
31	13264.0
32	14647.5
33	16031.0
34	17590.5
35	19150.0
36	21272.0
37	23394.0
38	25252.5
39	27111.0
40	30538.0
41	33965.0
42	37180.0
43	40395.0
44	44112.5
45	47830.0
46	66097.0
47	84364.0
48	79747.5
49	75131.0
50	72875.0
51	70619.0
52	63228.0
53	55837.0
54	52042.0
55	48247.0
56	44660.5
57	41074.0
58	39195.0
59	37316.0
60	37308.5
61	37301.0
62	33862.0
63	30423.0
64	26115.0
65	21807.0
66	17625.5
67	13444.0
68	11521.0
69	9598.0
70	7616.0
71	5634.0
72	4479.5
73	3325.0
74	2734.0
75	1771.0
76	1399.0
77	955.0
78	511.0
79	362.5
80	214.0
81	158.0
82	102.0
83	66.5
84	31.0
85	19.0
86	7.0
87	4.0
88	1.0
89	2.5
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	811768.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.693483309585915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.53487009305042	20.64130180904046
2	10.046895276594103	5.564670533110327
3	3.5992224136281115	2.990250175178929
4	1.7437652739418177	1.9316373803897298
5	1.0701898744867795	1.4818642713593737
6	0.7001499967051076	1.1633755348775718
7	0.5405031737838817	1.0477890935373502
8	0.4283385573327901	0.9489749350678185
9	0.3770799828430102	0.9398392390117662
>10	6.175794823981094	43.20855912674299
>50	0.7236340208096674	12.207874200128115
>100	0.0523918195688482	2.841555310067783
>500	8.955866592965505E-4	0.190850729780672
>1k	0.004925726626131027	2.492592377252441
>5k	0.0013433799889448256	2.348865284454651
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	7665	0.9442352987553094	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC	6007	0.7399897507662287	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	5269	0.6490770762089662	No Hit
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	3407	0.4197011954154389	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3311	0.4078751564486405	No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	2010	0.2476076908673414	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	1843	0.22703531058134835	TruSeq Adapter, Index 21 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCGTGTTT	1803	0.22210779434518238	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCT	1481	0.18244128864404607	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCC	1454	0.17911521518463402	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCT	1256	0.15472400981561235	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTA	1245	0.15336894285066668	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTC	1242	0.15299937913295425	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTT	1048	0.12910092538754914	No Hit
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	838	0.10323146514767767	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2318790590414995E-4	0.0	0.0	0.23368745750017247	0.0
2	1.2318790590414995E-4	0.0	0.0	1.1009303150653882	0.0
3	1.2318790590414995E-4	0.0	0.0	1.3773639759143006	0.0
4	1.2318790590414995E-4	0.0	0.0	1.8415360053611376	0.0
5	1.2318790590414995E-4	0.0	0.0	3.429674488277439	0.0
6	1.2318790590414995E-4	0.0	0.0	4.1005558238314395	0.0
7	1.2318790590414995E-4	0.0	0.0	4.819973194311675	0.0
8	1.2318790590414995E-4	0.0	0.0	5.711730445151817	0.0
9	1.2318790590414995E-4	0.0	0.0	6.029555242384523	0.0
10	1.2318790590414995E-4	0.0	0.0	7.692099220466932	0.0
11	1.2318790590414995E-4	0.0	0.0	8.824319263631974	0.0
12	1.2318790590414995E-4	0.0	0.0	10.310950912083255	0.0
13	1.2318790590414995E-4	0.0	0.0	10.702195701234835	0.0
14	1.2318790590414995E-4	0.0	0.0	10.893999270727598	0.0
15	1.2318790590414995E-4	0.0	0.0	11.332917779464083	0.0
16	1.2318790590414995E-4	0.0	0.0	11.888248859279992	0.0
17	1.2318790590414995E-4	0.0	0.0	12.526978151393008	0.0
18	1.2318790590414995E-4	0.0	0.0	13.164845128164698	0.0
19	1.2318790590414995E-4	0.0	0.0	13.82303810941057	0.0
20	1.2318790590414995E-4	0.0	0.0	14.266637758571415	0.0
21	1.2318790590414995E-4	0.0	0.0	14.763577770988755	0.0
22	1.2318790590414995E-4	0.0	0.0	15.319771166145992	0.0
23	1.2318790590414995E-4	0.0	0.0	15.798848932207232	0.0
24	1.2318790590414995E-4	0.0	0.0	16.21497767835145	0.0
25	1.2318790590414995E-4	0.0	0.0	16.596244247124794	0.0
26	1.2318790590414995E-4	0.0	0.0	16.958663066294804	0.0
27	1.2318790590414995E-4	0.0	0.0	17.3295818509722	0.0
28	1.2318790590414995E-4	0.0	0.0	17.682392013481685	0.0
29	1.2318790590414995E-4	0.0	0.0	18.03581811552069	0.0
30	1.2318790590414995E-4	0.0	0.0	18.414128174552335	0.0
31	1.2318790590414995E-4	0.0	0.0	18.785416522947443	0.0
32	1.2318790590414995E-4	0.0	0.0	19.136994806397887	0.0
33	1.2318790590414995E-4	0.0	0.0	19.496087552108484	0.0
34	1.2318790590414995E-4	0.0	0.0	19.8438470104759	0.0
35	1.2318790590414995E-4	0.0	0.0	20.2237585122843	0.0
36	1.2318790590414995E-4	0.0	0.0	20.603546826186793	0.0
37	1.2318790590414995E-4	0.0	0.0	20.95586423707266	0.0
38	1.2318790590414995E-4	0.0	0.0	21.313725103724217	0.0
39	1.2318790590414995E-4	0.0	0.0	21.699180061298303	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGCG	30	2.1647102E-6	45.000004	1
GTACGAG	30	2.1647102E-6	45.000004	1
CTAGACG	25	3.8897902E-5	45.0	1
CGAAACG	20	7.0320605E-4	45.0	12
TAGACGG	75	0.0	45.0	2
ACCGCAT	20	7.0320605E-4	45.0	14
ACGGGTC	45	3.8380676E-10	45.0	5
AGTATCG	20	7.0320605E-4	45.0	1
TTGCGAC	25	3.8897902E-5	45.0	29
CGTGTTA	20	7.0320605E-4	45.0	31
CGTAAGC	50	2.1827873E-11	45.0	43
CCGGTTG	20	7.0320605E-4	45.0	19
CGATCGA	45	3.8380676E-10	45.0	41
CGATACC	20	7.0320605E-4	45.0	44
AGTACGG	55	1.8189894E-12	45.0	2
CGATGAA	1130	0.0	41.615047	19
CCGATGA	1115	0.0	41.367714	18
ACACGAC	60	3.6379788E-12	41.250004	26
ACTACGG	55	6.002665E-11	40.909092	2
GATGAAT	1255	0.0	40.51793	20
>>END_MODULE