FastQCFastQC Report
Sat 18 Jun 2016
SRR3551312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432289
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32460.7508865596857658No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG26430.6113965425907205No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC26010.6016808200069861RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC21580.4992030794214056No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC14820.3428262111689171RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC12270.28383789548195765No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT10070.23294601528144365RNA PCR Primer, Index 38 (96% over 26bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8410.194545778402874No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC7990.1848300558191395RNA PCR Primer, Index 38 (96% over 25bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC6690.15475758115519941No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA6570.15198166041698954No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT6520.1508250267760688RNA PCR Primer, Index 38 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5980.13833338345412444No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT5900.13648276962865122No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC5290.12237183920941778RNA PCR Primer, Index 27 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT5260.1216778590248653RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGT5210.12052122538394454No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC4810.11126815625657836No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA4750.10988019588747343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACA351.2095734E-745.00000444
GTCGATG302.1623473E-645.0000041
GCGTTAG302.1623473E-645.0000041
CGTAGGT302.1623473E-645.0000042
TTAAGCG351.2095734E-745.0000041
AGTCTAG351.2095734E-745.0000041
ATCGCTC302.1623473E-645.00000433
AGTAACG302.1623473E-645.0000041
TTGATCG207.0283987E-445.01
GTCGAAT253.8867533E-545.043
CGCGGTA207.0283987E-445.030
CGACGTT207.0283987E-445.027
CAACGAT207.0283987E-445.012
TTAGCGG453.8380676E-1045.02
AACCCGC207.0283987E-445.034
TTTCGTC207.0283987E-445.018
GCAACGA207.0283987E-445.011
TTTCGAC207.0283987E-445.024
TAGCGAG253.8867533E-545.01
TTATCTA253.8867533E-545.012
Produced by FastQC (version 0.11.5)