##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551312_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	432289
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.584618160536124	31.0	31.0	34.0	30.0	34.0
2	31.7374418502437	31.0	31.0	34.0	30.0	34.0
3	31.67488184987358	33.0	31.0	34.0	30.0	34.0
4	35.37662073288934	37.0	35.0	37.0	33.0	37.0
5	33.35226202841155	37.0	35.0	37.0	30.0	37.0
6	34.267330420158736	37.0	35.0	37.0	30.0	37.0
7	35.337764782356246	37.0	35.0	37.0	32.0	37.0
8	35.13530762984948	37.0	35.0	37.0	32.0	37.0
9	37.001249164332194	39.0	37.0	39.0	33.0	39.0
10	36.61982608856575	38.0	35.0	39.0	32.0	39.0
11	36.78227528343307	39.0	37.0	39.0	33.0	39.0
12	36.70646257480528	39.0	35.0	39.0	33.0	39.0
13	36.73488106336271	39.0	35.0	39.0	33.0	39.0
14	37.72794357478446	40.0	37.0	41.0	33.0	41.0
15	37.886136357853196	40.0	37.0	41.0	33.0	41.0
16	37.948694044956035	40.0	37.0	41.0	33.0	41.0
17	37.79603922376003	40.0	36.0	41.0	33.0	41.0
18	37.76564983147848	39.0	36.0	41.0	33.0	41.0
19	37.658332735739286	39.0	36.0	41.0	33.0	41.0
20	37.73550333226152	39.0	36.0	41.0	33.0	41.0
21	37.61746655593827	39.0	35.0	41.0	33.0	41.0
22	37.756387509281986	39.0	36.0	41.0	33.0	41.0
23	37.8205159048692	40.0	36.0	41.0	33.0	41.0
24	37.683061562982175	39.0	35.0	41.0	33.0	41.0
25	37.391127232013766	39.0	35.0	41.0	33.0	41.0
26	37.50153716610878	39.0	35.0	41.0	33.0	41.0
27	37.088612016498224	39.0	35.0	41.0	33.0	41.0
28	37.12333415839866	39.0	35.0	41.0	32.0	41.0
29	37.36936401342621	39.0	35.0	41.0	33.0	41.0
30	37.09093453684919	39.0	35.0	41.0	32.0	41.0
31	37.07135504257568	39.0	35.0	41.0	32.0	41.0
32	37.10933657807624	39.0	35.0	41.0	32.0	41.0
33	37.08183414336243	40.0	35.0	41.0	32.0	41.0
34	36.94696372102922	40.0	35.0	41.0	32.0	41.0
35	36.89331442622875	40.0	35.0	41.0	31.0	41.0
36	36.65454591719891	39.0	35.0	41.0	31.0	41.0
37	36.652278915262706	39.0	35.0	41.0	31.0	41.0
38	36.51809784657949	39.0	35.0	41.0	31.0	41.0
39	36.470615722352406	39.0	35.0	41.0	31.0	41.0
40	36.379727450848854	39.0	35.0	41.0	30.0	41.0
41	36.18229702814552	39.0	35.0	41.0	30.0	41.0
42	36.34933574530002	39.0	35.0	41.0	30.0	41.0
43	36.24017266226992	39.0	35.0	41.0	30.0	41.0
44	36.31282082125615	39.0	35.0	41.0	30.0	41.0
45	36.319254017566955	39.0	35.0	41.0	30.0	41.0
46	36.20328530219367	39.0	35.0	41.0	30.0	41.0
47	36.107081142476446	39.0	35.0	41.0	30.0	41.0
48	36.10130491407369	39.0	35.0	41.0	30.0	41.0
49	36.058747735889646	39.0	35.0	40.0	30.0	41.0
50	35.97592120086331	38.0	35.0	40.0	30.0	41.0
51	35.08335164669931	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	17.0
9	26.0
10	40.0
11	25.0
12	14.0
13	24.0
14	29.0
15	50.0
16	59.0
17	94.0
18	170.0
19	331.0
20	576.0
21	973.0
22	1426.0
23	1920.0
24	2580.0
25	3520.0
26	4280.0
27	4989.0
28	5255.0
29	5847.0
30	6933.0
31	8966.0
32	12125.0
33	17327.0
34	29560.0
35	36836.0
36	36051.0
37	51621.0
38	86356.0
39	114227.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54628038187416	21.234868340392655	23.432703584870307	13.786147692862876
2	33.25553044375406	27.057593415516006	23.661948372500806	16.024927768229126
3	30.692430295473628	25.98955791148977	27.550319346548257	15.767692446488343
4	28.69469267087527	23.336471665945698	30.469662656232288	17.49917300694674
5	26.403170101483038	32.42992535086481	23.386900892689845	17.780003654962307
6	25.5671553058255	35.12927694204572	25.70016817453139	13.603399577597393
7	73.58225631464136	6.935869291145506	14.365852473692367	5.116021920520763
8	72.664814510663	9.572762665716684	12.240422495136356	5.522000328483954
9	66.79813735718466	8.993057884887195	14.595791241507417	9.613013516420727
10	40.178214111393075	25.11144165130272	20.824957378050332	13.88538685925388
11	31.095632782698612	24.08944016618512	26.468866892287334	18.34606015882893
12	28.281774461066554	21.476142117888728	29.71669415599287	20.525389265051853
13	23.953188723284654	25.489660851883812	31.651742237253316	18.905408187578217
14	18.173027766147186	30.24134317551453	29.91748575605671	21.668143302281575
15	18.640770410535545	25.368676973043495	36.01548963771921	19.97506297870175
16	19.511946868877068	25.363125131567077	33.14426228749748	21.980665712058368
17	19.86934666392159	27.678705680690463	29.114319355801328	23.33762829958662
18	20.60982352083907	25.839195538170067	31.614267307287484	21.936713633703377
19	23.354052497287693	26.315497271501243	28.36088820210554	21.96956202910553
20	25.081600503366964	26.478119961414702	30.300331491201487	18.139948044016847
21	23.64390488770244	27.84549225171124	30.333179886603638	18.177422973982683
22	20.94964248454159	25.227567668851165	30.246663690262764	23.576126156344483
23	21.389163268091487	27.415687190745082	29.74075213572402	21.454397405439416
24	21.60221518474909	25.584042156982946	31.266120581370334	21.547622076897632
25	19.78537506159074	29.391911429622315	28.730085660287447	22.0926278484995
26	20.642440589513033	27.68032496778775	28.79069326307169	22.886541179627518
27	21.945504049374374	30.33179192623453	28.34793390532722	19.374770119063868
28	19.364591743023766	27.37566766676922	31.94344524149354	21.316295348713478
29	20.60704760010086	25.928718981977333	30.51222677421817	22.952006643703633
30	20.886952941203685	29.716462829264685	28.937585735468634	20.458998494063
31	24.126221115966402	28.04697783195964	25.736486470856303	22.09031458121766
32	23.743606707549812	28.116607177143067	26.498939366951273	21.640846748355848
33	23.467865247554297	27.728209600521875	27.116119077746596	21.687806074177228
34	22.17058495589756	26.625243760539824	27.921598745283827	23.28257253827879
35	22.39520320896437	25.899340487497945	28.875821499043465	22.829634804494216
36	24.297402894822675	28.27043945138553	27.313209450159498	20.118948203632293
37	21.74841367696148	29.065046762698106	29.019475397245824	20.167064163094597
38	21.92005810927412	26.721013026008062	28.09532511815013	23.26360374656769
39	22.574712750035278	26.854951201626694	29.02525856545043	21.545077482887606
40	21.88605308023105	25.85839565660935	31.003564744881317	21.251986518278283
41	20.340559209232712	26.878083874445103	29.42684176557812	23.354515150744064
42	22.661460273104336	26.059187256673198	29.753937759230514	21.525414710991953
43	24.497269187973785	24.779950449814823	28.11452523658941	22.60825512562198
44	21.416691148745397	26.193819412476376	28.07034183150624	24.319147607271987
45	21.482156612821516	26.372866300090912	27.719650511579058	24.425326575508514
46	23.014002206856986	28.284087728348396	27.162153096655246	21.539756968139372
47	20.025029551989526	26.484365783075674	32.373481629187886	21.117123035746918
48	21.620952649732008	24.808634964109658	30.58208744612979	22.988324940028544
49	20.94339666288062	24.02975787031361	32.12133549546715	22.90550997133862
50	20.807145219980153	25.04990874160573	30.624188910659306	23.518757127754814
51	20.204307766332246	24.872249814360302	28.1205397315222	26.802902687785256
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	476.0
1	785.0
2	1094.0
3	3196.5
4	5299.0
5	3511.5
6	1724.0
7	1734.5
8	1745.0
9	1705.5
10	1666.0
11	1685.5
12	1705.0
13	1707.0
14	1709.0
15	1646.5
16	1584.0
17	1548.0
18	1512.0
19	1605.0
20	1698.0
21	2298.0
22	2898.0
23	2750.5
24	2603.0
25	3069.0
26	4281.5
27	5028.0
28	6110.0
29	7192.0
30	7586.0
31	7980.0
32	8680.0
33	9380.0
34	10978.0
35	12576.0
36	13212.5
37	13849.0
38	14121.0
39	14393.0
40	16870.0
41	19347.0
42	22824.0
43	26301.0
44	28206.0
45	30111.0
46	37500.0
47	44889.0
48	42063.0
49	39237.0
50	38045.0
51	36853.0
52	32327.5
53	27802.0
54	24539.5
55	21277.0
56	19905.5
57	18534.0
58	17153.5
59	15773.0
60	15318.0
61	14863.0
62	13799.5
63	12736.0
64	10883.5
65	9031.0
66	7623.0
67	6215.0
68	4979.5
69	3744.0
70	3189.5
71	2635.0
72	2003.5
73	1372.0
74	1233.0
75	756.5
76	419.0
77	348.5
78	278.0
79	166.5
80	55.0
81	57.0
82	59.0
83	33.5
84	8.0
85	5.5
86	3.0
87	2.5
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	432289.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.118089833103994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.52236658972905	22.745894213580712
2	10.003895081098198	6.025964214669254
3	3.6675435674842007	3.3137821989693554
4	1.8223312984676883	2.1954055101170757
5	0.994088241826075	1.4970019484675066
6	0.6951376920732059	1.2561731673742438
7	0.5027501143355366	1.0599311175012698
8	0.38418484627827404	0.9256730970181044
9	0.3431217684780688	0.9300755022044388
>10	5.3860914564653	41.04363276817621
>50	0.6049088784018405	10.953847543000649
>100	0.060413434718749756	3.2131161713331546
>500	0.0077453121434294565	1.4887547121713511
>1k	0.005421718500400619	3.3507478354166857
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3246	0.7508865596857658	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	2643	0.6113965425907205	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	2601	0.6016808200069861	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	2158	0.4992030794214056	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1482	0.3428262111689171	RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	1227	0.28383789548195765	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	1007	0.23294601528144365	RNA PCR Primer, Index 38 (96% over 26bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	841	0.194545778402874	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	799	0.1848300558191395	RNA PCR Primer, Index 38 (96% over 25bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	669	0.15475758115519941	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	657	0.15198166041698954	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	652	0.1508250267760688	RNA PCR Primer, Index 38 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	598	0.13833338345412444	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	590	0.13648276962865122	No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	529	0.12237183920941778	RNA PCR Primer, Index 27 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	526	0.1216778590248653	RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGT	521	0.12052122538394454	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC	481	0.11126815625657836	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA	475	0.10988019588747343	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.313267281841546E-4	0.0	0.0	0.2419677576806257	0.0
2	2.313267281841546E-4	0.0	0.0	1.0152930100002544	0.0
3	2.313267281841546E-4	0.0	0.0	1.3463215580317798	0.0
4	2.313267281841546E-4	0.0	0.0	1.8746718052043887	0.0
5	2.313267281841546E-4	0.0	0.0	3.5776991780961347	0.0
6	2.313267281841546E-4	0.0	0.0	4.379246291254231	0.0
7	2.313267281841546E-4	0.0	0.0	5.167145127449461	0.0
8	2.313267281841546E-4	0.0	0.0	6.265484432867827	0.0
9	2.313267281841546E-4	0.0	0.0	6.623578208096898	0.0
10	2.313267281841546E-4	0.0	0.0	8.102681308106382	0.0
11	2.313267281841546E-4	0.0	0.0	9.588261556505023	0.0
12	2.313267281841546E-4	0.0	0.0	11.193437723374872	0.0
13	2.313267281841546E-4	0.0	0.0	11.702587852108197	0.0
14	2.313267281841546E-4	0.0	0.0	11.944555609788821	0.0
15	2.313267281841546E-4	0.0	0.0	12.390322214999689	0.0
16	2.313267281841546E-4	0.0	0.0	13.056080538713685	0.0
17	2.313267281841546E-4	0.0	0.0	13.787072999775614	0.0
18	2.313267281841546E-4	0.0	0.0	14.604350330450231	0.0
19	2.313267281841546E-4	0.0	0.0	15.22685055599379	0.0
20	2.313267281841546E-4	0.0	0.0	15.736463338183484	0.0
21	2.313267281841546E-4	0.0	0.0	16.31686209919753	0.0
22	2.313267281841546E-4	0.0	0.0	16.895178919657916	0.0
23	2.313267281841546E-4	0.0	0.0	17.439953364531597	0.0
24	2.313267281841546E-4	0.0	0.0	17.91162856329909	0.0
25	2.313267281841546E-4	0.0	0.0	18.290541744064736	0.0
26	2.313267281841546E-4	0.0	0.0	18.698370765853397	0.0
27	2.313267281841546E-4	0.0	0.0	19.09764069869925	0.0
28	2.313267281841546E-4	0.0	0.0	19.47077071126029	0.0
29	2.313267281841546E-4	0.0	0.0	19.879525039961692	0.0
30	2.313267281841546E-4	0.0	0.0	20.315575922588824	0.0
31	2.313267281841546E-4	0.0	0.0	20.755559359595086	0.0
32	4.626534563683092E-4	0.0	0.0	21.172641450511115	0.0
33	4.626534563683092E-4	0.0	0.0	21.56474025478326	0.0
34	4.626534563683092E-4	0.0	0.0	21.974188563669212	0.0
35	4.626534563683092E-4	0.0	0.0	22.421574455977368	0.0
36	4.626534563683092E-4	0.0	0.0	22.846752982379844	0.0
37	4.626534563683092E-4	0.0	0.0	23.248336182507536	0.0
38	4.626534563683092E-4	0.0	0.0	23.60619863100842	0.0
39	4.626534563683092E-4	0.0	0.0	24.01587826662256	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACA	35	1.2095734E-7	45.000004	44
GTCGATG	30	2.1623473E-6	45.000004	1
GCGTTAG	30	2.1623473E-6	45.000004	1
CGTAGGT	30	2.1623473E-6	45.000004	2
TTAAGCG	35	1.2095734E-7	45.000004	1
AGTCTAG	35	1.2095734E-7	45.000004	1
ATCGCTC	30	2.1623473E-6	45.000004	33
AGTAACG	30	2.1623473E-6	45.000004	1
TTGATCG	20	7.0283987E-4	45.0	1
GTCGAAT	25	3.8867533E-5	45.0	43
CGCGGTA	20	7.0283987E-4	45.0	30
CGACGTT	20	7.0283987E-4	45.0	27
CAACGAT	20	7.0283987E-4	45.0	12
TTAGCGG	45	3.8380676E-10	45.0	2
AACCCGC	20	7.0283987E-4	45.0	34
TTTCGTC	20	7.0283987E-4	45.0	18
GCAACGA	20	7.0283987E-4	45.0	11
TTTCGAC	20	7.0283987E-4	45.0	24
TAGCGAG	25	3.8867533E-5	45.0	1
TTATCTA	25	3.8867533E-5	45.0	12
>>END_MODULE