##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551309_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	810510
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.602783432653514	31.0	31.0	34.0	30.0	34.0
2	31.747861223180468	33.0	31.0	34.0	30.0	34.0
3	31.688985947119715	33.0	31.0	34.0	30.0	34.0
4	35.36821137308608	37.0	35.0	37.0	33.0	37.0
5	33.36261242921124	37.0	35.0	37.0	30.0	37.0
6	34.28147832845986	37.0	35.0	37.0	30.0	37.0
7	35.378350668097866	37.0	35.0	37.0	32.0	37.0
8	35.21868329817029	37.0	35.0	37.0	32.0	37.0
9	37.107179430235284	39.0	37.0	39.0	34.0	39.0
10	36.67576340822445	39.0	35.0	39.0	32.0	39.0
11	36.78482560363228	39.0	37.0	39.0	33.0	39.0
12	36.76184624495688	39.0	35.0	39.0	33.0	39.0
13	36.84139739176568	39.0	37.0	39.0	33.0	39.0
14	37.830504250410236	40.0	37.0	41.0	33.0	41.0
15	37.96272347040752	40.0	37.0	41.0	33.0	41.0
16	37.99954719867738	40.0	37.0	41.0	33.0	41.0
17	37.879415429791116	40.0	37.0	41.0	33.0	41.0
18	37.83950969142885	39.0	37.0	41.0	33.0	41.0
19	37.74466940568284	39.0	36.0	41.0	33.0	41.0
20	37.8969093533701	40.0	36.0	41.0	34.0	41.0
21	37.77645926638783	39.0	36.0	41.0	33.0	41.0
22	37.937568938076026	40.0	36.0	41.0	34.0	41.0
23	38.00783827466657	40.0	36.0	41.0	34.0	41.0
24	37.840205549592234	40.0	36.0	41.0	33.0	41.0
25	37.54166018926355	39.0	35.0	41.0	33.0	41.0
26	37.634417835683706	39.0	36.0	41.0	33.0	41.0
27	37.27618906614354	40.0	35.0	41.0	33.0	41.0
28	37.33547519463054	39.0	35.0	41.0	33.0	41.0
29	37.54375640029117	40.0	35.0	41.0	33.0	41.0
30	37.25743544188227	39.0	35.0	41.0	32.0	41.0
31	37.226436441253036	39.0	35.0	41.0	33.0	41.0
32	37.29808145488643	39.0	35.0	41.0	33.0	41.0
33	37.27824456206586	40.0	35.0	41.0	33.0	41.0
34	37.175179825048424	40.0	35.0	41.0	32.0	41.0
35	37.13575526520339	40.0	35.0	41.0	32.0	41.0
36	36.93835979815178	39.0	35.0	41.0	32.0	41.0
37	36.93686444337516	39.0	35.0	41.0	32.0	41.0
38	36.75719238504152	39.0	35.0	41.0	31.0	41.0
39	36.695790304869774	39.0	35.0	41.0	31.0	41.0
40	36.60421709787664	39.0	35.0	41.0	31.0	41.0
41	36.44294333197616	39.0	35.0	41.0	31.0	41.0
42	36.631745444226475	39.0	35.0	41.0	31.0	41.0
43	36.561050449716845	39.0	35.0	41.0	31.0	41.0
44	36.614878286510965	39.0	35.0	41.0	31.0	41.0
45	36.59674896053102	39.0	35.0	41.0	31.0	41.0
46	36.4646457168943	39.0	35.0	41.0	31.0	41.0
47	36.40966305165883	39.0	35.0	41.0	31.0	41.0
48	36.37808787059999	39.0	35.0	41.0	31.0	41.0
49	36.33083243883481	39.0	35.0	41.0	31.0	41.0
50	36.276639399884026	39.0	35.0	41.0	31.0	41.0
51	35.338011869070094	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	19.0
9	33.0
10	46.0
11	47.0
12	66.0
13	49.0
14	62.0
15	97.0
16	96.0
17	176.0
18	296.0
19	483.0
20	727.0
21	1219.0
22	1880.0
23	2757.0
24	3808.0
25	5481.0
26	7392.0
27	8624.0
28	9403.0
29	10506.0
30	12527.0
31	16279.0
32	21375.0
33	31370.0
34	55259.0
35	65247.0
36	69821.0
37	100195.0
38	164912.0
39	220207.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1359391000728	21.612564928255047	22.503238701558278	13.748257270113879
2	34.46089499204204	24.610183711490297	23.378613465595734	17.55030783087192
3	32.63044256085674	23.68669109572985	26.828046538599153	16.85481980481425
4	30.33164303956768	25.145649035792278	26.947230755943792	17.575477168696253
5	25.14614255222021	33.96762532232792	23.398724260033806	17.48750786541807
6	26.214975756005476	34.968229879952126	24.637080356812376	14.179714007230015
7	75.06569937446793	7.564619807281835	12.112250311532245	5.257430506717992
8	75.5036952042541	8.034200688455417	11.098197431246993	5.363906676043479
9	68.6313555662484	9.785690491172225	13.977495650886478	7.605458291692885
10	39.896114791921136	26.6039900803198	20.188276517254568	13.311618610504498
11	31.601954325054592	25.016347731675122	25.337626926256306	18.04407101701398
12	29.308953621793687	21.792204908020878	29.226412999222713	19.67242847096273
13	25.597339946453467	24.28125501227622	29.691305474330974	20.430099566939333
14	19.441709540906345	28.218529074286558	30.569024441401094	21.770736943406003
15	19.952128906490973	26.425090375195865	33.966268152151116	19.656512566162046
16	22.374307534762064	26.096408434195755	31.141256739583717	20.388027291458464
17	22.333345671244032	25.992399847009906	29.204328139072928	22.469926342673133
18	21.965675932437602	26.595230163724075	29.99629862679054	21.442795277047786
19	24.07397811254642	26.572034891611455	28.345732933585026	21.008254062257098
20	25.34614008463807	26.306893190707086	29.741520770872658	18.605445953782187
21	24.11839459105995	27.07714895559586	29.426657289854536	19.377799163489655
22	22.74481499302908	25.113200330656007	29.16645075322945	22.975533923085464
23	22.89595439908206	25.489506606951178	30.079949661324353	21.53458933264241
24	22.346793993905074	25.211410099813698	30.397157345375135	22.044638560906098
25	21.743470160762975	27.16314419316233	27.876892327053337	23.216493319021357
26	20.240959395935892	27.835313568000398	28.697856904911724	23.22587013115199
27	21.498315875189693	27.89712650059839	29.44158616179936	21.162971462412557
28	19.776683816362535	27.957829021233543	30.166685173532713	22.098801988871205
29	22.181836127870106	25.605112830193335	28.728084786122317	23.48496625581424
30	21.360624791797758	27.068759176321084	28.749182613416245	22.821433418464917
31	23.110757424337763	26.717375479636278	26.92662644507779	23.24524065094817
32	23.73012054138752	28.053571208251594	26.129350655759954	22.086957594600932
33	22.18590763840051	28.393357268880088	26.60843172817115	22.81230336454825
34	21.730021838101933	26.547729207535998	28.652453393542338	23.06979556081973
35	21.882641793438697	26.605470629603584	27.0480314863481	24.463856090609617
36	21.545693452270793	29.05047439266635	26.147240626272346	23.256591528790516
37	22.523966391531257	28.181762100405916	28.24962060924603	21.044650898816794
38	22.25450642188252	29.015928242711382	26.082219836892822	22.647345498513282
39	23.312112126932423	26.61386040887836	27.91847108610628	22.155556378082935
40	21.47240626272347	27.206203501499054	29.94633008846282	21.375060147314652
41	19.58532282143342	28.098111065872107	28.64961567408175	23.666950438612723
42	20.428248880334603	27.781026760928306	28.260601349767427	23.53012300896966
43	22.584792291273395	26.5785739842815	27.412493368373	23.424140356072105
44	21.56395356010413	26.85346263463745	27.863814141713245	23.718769663545174
45	22.515823370470443	26.01497822358762	26.630393209213953	24.838805196727986
46	21.971968266893686	28.400019740657118	26.86740447372642	22.760607518722782
47	21.375060147314652	26.062849317096642	29.76039777424091	22.801692761347795
48	20.967168819632086	26.332926182280293	29.247387447409658	23.452517550677968
49	20.763716672218727	26.300477477144018	29.91141380118691	23.024392049450345
50	20.30844776745506	25.242995151201097	29.91203069672182	24.53652638462203
51	20.23096568827035	25.194383783050178	28.004836460993694	26.569814067685776
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	536.0
1	1469.5
2	2403.0
3	6000.5
4	9598.0
5	6332.0
6	3066.0
7	2990.5
8	2915.0
9	2987.0
10	3059.0
11	2929.0
12	2799.0
13	2756.5
14	2714.0
15	2651.0
16	2588.0
17	2663.0
18	2738.0
19	2808.0
20	2878.0
21	3892.5
22	4907.0
23	4698.5
24	4490.0
25	5697.5
26	8792.0
27	10679.0
28	11900.0
29	13121.0
30	14377.0
31	15633.0
32	17653.0
33	19673.0
34	20949.0
35	22225.0
36	23828.5
37	25432.0
38	26346.0
39	27260.0
40	32324.5
41	37389.0
42	41332.0
43	45275.0
44	47777.5
45	50280.0
46	57817.5
47	65355.0
48	67906.5
49	70458.0
50	67873.5
51	65289.0
52	57450.5
53	49612.0
54	45855.0
55	42098.0
56	40186.0
57	38274.0
58	37519.0
59	36764.0
60	35455.5
61	34147.0
62	30521.0
63	26895.0
64	23979.0
65	21063.0
66	17826.5
67	14590.0
68	12007.0
69	9424.0
70	8173.0
71	6922.0
72	5807.0
73	4692.0
74	3802.5
75	2337.5
76	1762.0
77	1394.5
78	1027.0
79	787.5
80	548.0
81	300.5
82	53.0
83	45.0
84	37.0
85	23.5
86	10.0
87	6.0
88	2.0
89	2.5
90	3.0
91	3.5
92	4.0
93	4.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	810510.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.615151596458936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.50237076687807	20.297791114264694
2	10.908420794845263	6.02475387855234
3	4.105463786795404	3.4011901453838234
4	2.0297693298027997	2.2420955099338866
5	1.182388877686225	1.6325924051636025
6	0.6941403365578063	1.1501274373956512
7	0.5200433606171022	1.0052753368121268
8	0.41450008731717003	0.9157186198407292
9	0.3190837799005161	0.79303922585315
>10	5.2547197606807865	38.01852312962475
>50	0.9836790883999832	16.977034555757143
>100	0.07552929014296957	3.901599250419023
>500	0.004046211971944798	0.7554646292496582
>1k	0.0053949492959263975	2.2200852095714687
>5k	4.4957910799386653E-4	0.6647095521779244
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5354	0.6605717387817547	No Hit
GCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	2361	0.2912980715845579	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCG	1863	0.22985527630751007	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCC	1641	0.20246511455750082	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTC	1623	0.20024429063182442	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGC	1600	0.1974065711712379	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1470	0.1813672872635748	No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT	1388	0.17125020049104886	No Hit
CCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	1352	0.166808552639696	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	1283	0.15829539425793634	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1169	0.14423017606198565	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1115	0.13756770428495638	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1017	0.12547655180071807	No Hit
TCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	855	0.10548913646963023	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2337910698202368E-4	0.0	0.0	0.1782828095890242	0.0
2	1.2337910698202368E-4	0.0	0.0	0.8403350976545632	0.0
3	1.2337910698202368E-4	0.0	0.0	1.1018988044564533	0.0
4	1.2337910698202368E-4	0.0	0.0	1.4774648061097333	0.0
5	1.2337910698202368E-4	0.0	0.0	2.637721928168684	0.0
6	1.2337910698202368E-4	0.0	0.0	3.221798620621584	0.0
7	1.2337910698202368E-4	0.0	0.0	3.860038741039592	0.0
8	1.2337910698202368E-4	0.0	0.0	4.738868120072547	0.0
9	1.2337910698202368E-4	0.0	0.0	5.071621571603065	0.0
10	1.2337910698202368E-4	0.0	0.0	6.077408051720521	0.0
11	1.2337910698202368E-4	0.0	0.0	7.371284746641003	0.0
12	1.2337910698202368E-4	0.0	0.0	8.510073904085083	0.0
13	1.2337910698202368E-4	0.0	0.0	8.913153446595354	0.0
14	1.2337910698202368E-4	0.0	0.0	9.094767492072892	0.0
15	1.2337910698202368E-4	0.0	0.0	9.467865911586532	0.0
16	1.2337910698202368E-4	0.0	0.0	10.077852216505656	0.0
17	1.2337910698202368E-4	0.0	0.0	10.772970105242377	0.0
18	1.2337910698202368E-4	0.0	0.0	11.529160651935202	0.0
19	1.2337910698202368E-4	0.0	0.0	12.067833833018717	0.0
20	1.2337910698202368E-4	0.0	0.0	12.571467347719338	0.0
21	1.2337910698202368E-4	0.0	0.0	13.157888243204896	0.0
22	1.2337910698202368E-4	0.0	0.0	13.785887897743397	0.0
23	1.2337910698202368E-4	0.0	0.0	14.356269509321292	0.0
24	1.2337910698202368E-4	0.0	0.0	14.836214235481364	0.0
25	1.2337910698202368E-4	0.0	0.0	15.280255641509667	0.0
26	3.7013732094607097E-4	0.0	0.0	15.697770539536835	0.0
27	3.7013732094607097E-4	0.0	0.0	16.083083490641695	0.0
28	3.7013732094607097E-4	0.0	0.0	16.507260860445893	0.0
29	3.7013732094607097E-4	0.0	0.0	16.932055125785	0.0
30	3.7013732094607097E-4	0.0	0.0	17.41064268176827	0.0
31	3.7013732094607097E-4	0.0	0.0	17.86924282242045	0.0
32	4.935164279280947E-4	0.0	0.0	18.34412900519426	0.0
33	4.935164279280947E-4	0.0	0.0	18.790267856041257	0.0
34	4.935164279280947E-4	0.0	0.0	19.213088055668653	0.0
35	4.935164279280947E-4	0.0	0.0	19.644421413677808	0.0
36	4.935164279280947E-4	0.0	0.0	20.097716252729764	0.0
37	4.935164279280947E-4	0.0	0.0	20.526952165920225	0.0
38	4.935164279280947E-4	0.0	0.0	20.956681595538612	0.0
39	4.935164279280947E-4	0.0	0.0	21.38530061319416	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	20	7.0320553E-4	45.0	10
CGCATCG	25	3.8897848E-5	45.0	21
GGCGTAT	20	7.0320553E-4	45.0	33
GGTATCG	20	7.0320553E-4	45.0	12
CGAATCA	25	3.8897848E-5	45.0	43
AATTCGC	20	7.0320553E-4	45.0	37
CGGCTAT	25	3.8897848E-5	45.0	28
CCGAAAC	20	7.0320553E-4	45.0	29
TGTAACG	40	6.8102963E-9	45.0	1
TACGTAT	20	7.0320553E-4	45.0	37
TGCGTAA	20	7.0320553E-4	45.0	26
CTACGCG	30	2.1647065E-6	44.999996	33
CGTAAGG	115	0.0	41.086956	2
TCGTAAG	50	1.0804797E-9	40.5	1
GGCGTAG	70	0.0	38.571426	1
ACTACGC	35	6.2460113E-6	38.571426	32
TTACGAG	35	6.2460113E-6	38.571426	1
TTAATCG	60	1.546141E-10	37.499996	20
TTTCGGC	30	1.1396251E-4	37.499996	25
TAGCCGT	30	1.1396251E-4	37.499996	44
>>END_MODULE