##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551307_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556901
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.622634902792417	31.0	31.0	34.0	30.0	34.0
2	31.76752241421725	31.0	31.0	34.0	30.0	34.0
3	31.725681943469308	33.0	31.0	34.0	30.0	34.0
4	35.41524076990345	37.0	35.0	37.0	33.0	37.0
5	33.42930969777393	37.0	35.0	37.0	30.0	37.0
6	34.31480640185598	37.0	35.0	37.0	30.0	37.0
7	35.39543473615598	37.0	35.0	37.0	32.0	37.0
8	35.23009834782125	37.0	35.0	37.0	32.0	37.0
9	37.06180631746037	39.0	37.0	39.0	33.0	39.0
10	36.630234099058896	38.0	35.0	39.0	32.0	39.0
11	36.74622419424637	39.0	37.0	39.0	33.0	39.0
12	36.69720830093679	39.0	35.0	39.0	33.0	39.0
13	36.758163479684896	39.0	35.0	39.0	33.0	39.0
14	37.80765522058678	40.0	37.0	41.0	33.0	41.0
15	37.89123740126163	40.0	37.0	41.0	33.0	41.0
16	37.98810021888989	40.0	37.0	41.0	33.0	41.0
17	37.79272438009628	40.0	36.0	41.0	33.0	41.0
18	37.78821370405153	39.0	36.0	41.0	33.0	41.0
19	37.69838983948673	39.0	36.0	41.0	33.0	41.0
20	37.74171351820162	40.0	36.0	41.0	33.0	41.0
21	37.69307830296588	39.0	36.0	41.0	33.0	41.0
22	37.79119807649834	40.0	36.0	41.0	33.0	41.0
23	37.82070242287229	40.0	36.0	41.0	33.0	41.0
24	37.6987633349554	40.0	36.0	41.0	33.0	41.0
25	37.468185548239276	39.0	35.0	41.0	33.0	41.0
26	37.50091488433312	39.0	35.0	41.0	33.0	41.0
27	37.0628190647889	39.0	35.0	41.0	32.0	41.0
28	37.09460747960589	39.0	35.0	41.0	32.0	41.0
29	37.32202312439733	40.0	35.0	41.0	32.0	41.0
30	37.06556461561391	39.0	35.0	41.0	32.0	41.0
31	37.08507616254954	39.0	35.0	41.0	32.0	41.0
32	37.033294966250736	39.0	35.0	41.0	32.0	41.0
33	37.02974137234445	40.0	35.0	41.0	32.0	41.0
34	36.931578503180994	40.0	35.0	41.0	31.0	41.0
35	36.82828366262585	40.0	35.0	41.0	31.0	41.0
36	36.65125938003344	39.0	35.0	41.0	31.0	41.0
37	36.60131693065733	39.0	35.0	41.0	31.0	41.0
38	36.45664848869009	39.0	35.0	41.0	30.0	41.0
39	36.40063853359933	39.0	35.0	41.0	30.0	41.0
40	36.32002456450967	39.0	35.0	41.0	30.0	41.0
41	36.161639142325114	39.0	35.0	41.0	30.0	41.0
42	36.250787842004236	39.0	35.0	41.0	30.0	41.0
43	36.2228582818131	39.0	35.0	41.0	30.0	41.0
44	36.378561000967856	39.0	35.0	41.0	30.0	41.0
45	36.3531471482364	39.0	35.0	41.0	30.0	41.0
46	36.21133199617167	39.0	35.0	41.0	30.0	41.0
47	36.102007358578994	39.0	35.0	41.0	30.0	41.0
48	36.06683593672843	39.0	35.0	41.0	30.0	41.0
49	36.01490390572113	39.0	35.0	41.0	30.0	41.0
50	35.96312989202749	39.0	35.0	41.0	30.0	41.0
51	34.97570124672069	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	17.0
10	29.0
11	33.0
12	38.0
13	30.0
14	35.0
15	48.0
16	65.0
17	111.0
18	218.0
19	451.0
20	895.0
21	1764.0
22	2575.0
23	3220.0
24	3758.0
25	4672.0
26	5430.0
27	6069.0
28	6423.0
29	7319.0
30	8918.0
31	11331.0
32	14795.0
33	21107.0
34	36980.0
35	44946.0
36	45750.0
37	66378.0
38	111547.0
39	151903.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.52640595007012	19.651428171254857	23.04107911460026	12.781086764074765
2	31.819300019213472	28.946437517619827	23.59270319141104	15.641559271755662
3	31.856110870693357	27.224048798619506	25.589467427783397	15.330372902903747
4	28.17825789502982	24.34867238521748	29.587844159015695	17.885225560737005
5	27.79470677912232	32.82737865437483	23.015042170870586	16.36287239563226
6	25.084530284556863	37.75231145212524	23.558406251739537	13.60475201157836
7	74.91062145695554	6.789896229311852	14.162122172522585	4.137360141210018
8	75.93450182348388	10.391254459948895	9.38299625965836	4.291247456908858
9	69.6409236111984	8.794920461626035	11.343129209679997	10.221026717495569
10	42.10335409704777	27.582281231314003	17.681419139128856	12.632945532509368
11	33.08397722395902	24.285824590007916	24.90029646202826	17.729901724004804
12	27.35118091007199	22.985234359428336	28.113973578786894	21.54961115171278
13	25.846604692755086	24.41888235072302	30.43090244046967	19.303610516052224
14	20.655197243316138	29.85431881070424	28.245774383597805	21.244709562381825
15	19.487126078064144	26.22620537582084	33.69108692568338	20.595581620431638
16	21.768680609300397	26.596648237298908	29.736164955710258	21.898506197690434
17	21.857924478497974	27.769388095909324	28.689838948035646	21.68284847755705
18	21.697393253019836	26.020064607533477	29.116126564685647	23.166415574761043
19	23.279362041009083	27.092966254325273	27.11182059288814	22.515851111777497
20	26.97642848549383	26.43001179742899	29.64961456345024	16.943945153626945
21	24.586775746497132	28.689479817777308	28.185440500196624	18.538303935528937
22	21.296963014970345	24.908376892840916	30.935121323179526	22.859538769009212
23	23.564870596389664	27.432523913586078	29.136237859152704	19.866367630871558
24	22.62071714721288	26.655904729925066	28.504348169602856	22.2190299532592
25	21.175936117909647	30.3181355393508	26.77944553879415	21.726482803945405
26	20.847152366399055	27.574021235372175	30.284556860195977	21.294269538032793
27	23.82541959881559	28.12977531015387	28.506143820894557	19.538661270135986
28	19.59648124172878	28.922016660052684	30.846057019111118	20.635445079107416
29	22.43989506213851	25.48154878515212	30.385472462789615	21.69308368991975
30	21.160313951671842	28.921477964665176	29.65338543116281	20.264822652500175
31	24.618738339489425	26.073395450897017	25.85145295124268	23.45641325837088
32	25.247036726455867	27.303775715971064	27.249008351574155	20.20017920599891
33	23.569539289748086	27.735629851625333	26.572406944860937	22.122423913765644
34	23.87785261653328	25.943569862506983	27.235540966886397	22.943036554073345
35	23.228904239712264	25.26176106704782	29.45783900549649	22.051495687743422
36	24.125472929658955	29.95846658562294	26.729526432884835	19.18653405183327
37	22.29372904699399	27.542597337767393	28.954158818174147	21.209514797064468
38	22.765805771582382	28.043943178410522	25.950034207157106	23.240216842849986
39	24.348313254959137	26.737068168309985	27.04663845099937	21.867980125731503
40	22.941779598169155	25.603114377600328	29.078597452689074	22.37650857154144
41	21.703678032540793	26.943927197114032	27.903164117141106	23.44923065320407
42	21.555536800975396	26.20124582286618	30.169276047268724	22.073941328889696
43	22.943754814590026	26.368780088381953	27.8532450112318	22.83422008579622
44	22.41511507431303	25.61604306690058	28.86545364436408	23.103388214422313
45	22.244348636472193	25.571331349737207	27.68068292209926	24.503637091691342
46	23.83655263682414	27.676732489257517	27.054359751553687	21.43235512236466
47	21.05724356752816	27.28276659585815	30.853239624277922	20.806750212335764
48	22.65124321917181	26.258706664200638	28.26229437548146	22.827755741146092
49	21.267334768657264	25.194424143609005	31.549234064941523	21.9890070227922
50	21.47940118620724	25.785193418578885	28.85234538993466	23.883060005279212
51	21.039107489481975	25.55157918552849	27.650515980398673	25.75879734459087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	325.0
1	882.0
2	1439.0
3	3203.0
4	4967.0
5	3401.0
6	1835.0
7	1770.0
8	1705.0
9	1680.0
10	1655.0
11	1615.0
12	1575.0
13	1586.5
14	1598.0
15	1640.0
16	1682.0
17	1693.0
18	1704.0
19	1766.0
20	1828.0
21	3283.0
22	4738.0
23	4160.0
24	3582.0
25	3945.0
26	5027.5
27	5747.0
28	6958.5
29	8170.0
30	9392.5
31	10615.0
32	11992.5
33	13370.0
34	14735.0
35	16100.0
36	17650.5
37	19201.0
38	20133.5
39	21066.0
40	23630.5
41	26195.0
42	28710.5
43	31226.0
44	32982.5
45	34739.0
46	45413.0
47	56087.0
48	51272.0
49	46457.0
50	45970.0
51	45483.0
52	40093.5
53	34704.0
54	31617.0
55	28530.0
56	26760.0
57	24990.0
58	24687.5
59	24385.0
60	23442.5
61	22500.0
62	19694.5
63	16889.0
64	14727.5
65	12566.0
66	10513.5
67	8461.0
68	7137.0
69	5813.0
70	5030.5
71	4248.0
72	3657.0
73	3066.0
74	2344.0
75	1193.0
76	764.0
77	671.0
78	578.0
79	416.0
80	254.0
81	154.0
82	54.0
83	51.0
84	48.0
85	38.0
86	28.0
87	14.5
88	1.0
89	0.5
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	556901.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.68345388626676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28519984697657	20.841543582821537
2	9.180855054870857	5.0831555509603294
3	3.2190568015529135	2.673438315691904
4	1.5804341767008616	1.750075066039131
5	0.9467558953975524	1.310473658589467
6	0.6790291236577797	1.1278722859327366
7	0.5547307607875507	1.0749804404889087
8	0.45258137989259767	1.0023212608031764
9	0.4200738284242975	1.046618501221042
>10	7.1884800644334685	49.280324519328694
>50	0.4192111425879201	6.896733944519815
>100	0.06242172185870284	2.9636947423990856
>500	0.006570707564073983	1.414453465715049
>1k	0.004599495294851788	3.5343146654891204
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCG	4351	0.7812878770194343	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC	4267	0.7662044061691395	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	3741	0.6717531482256272	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2707	0.48608280466366555	No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	1812	0.3253720140563583	TruSeq Adapter, Index 15 (95% over 23bp)
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	1320	0.23702597050463187	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGCAGTAT	1232	0.2212242391376564	No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	917	0.1646612234490511	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	908	0.1630451372865195	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTA	899	0.16142905112398792	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC	877	0.15747861828224408	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	870	0.15622166237805282	TruSeq Adapter, Index 15 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	792	0.14221558230277914	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTC	711	0.1276708068399949	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	692	0.12425906938576158	No Hit
AAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAAC	595	0.10684125185625452	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7956512917017566E-4	0.0	0.0	0.17363947990755987	0.0
2	1.7956512917017566E-4	0.0	0.0	0.7836222236986466	0.0
3	1.7956512917017566E-4	0.0	0.0	0.9678560462272469	0.0
4	1.7956512917017566E-4	0.0	0.0	1.3645154165641649	0.0
5	1.7956512917017566E-4	0.0	0.0	2.7130495366321843	0.0
6	1.7956512917017566E-4	0.0	0.0	3.1603462733950916	0.0
7	1.7956512917017566E-4	0.0	0.0	3.737289033418866	0.0
8	1.7956512917017566E-4	0.0	0.0	4.499543006746262	0.0
9	1.7956512917017566E-4	0.0	0.0	4.740698975221807	0.0
10	1.7956512917017566E-4	0.0	0.0	6.140229591974157	0.0
11	1.7956512917017566E-4	0.0	0.0	7.072531742625709	0.0
12	1.7956512917017566E-4	0.0	0.0	8.608172727289052	0.0
13	1.7956512917017566E-4	0.0	0.0	8.9432412583206	0.0
14	1.7956512917017566E-4	0.0	0.0	9.095512487856908	0.0
15	1.7956512917017566E-4	0.0	0.0	9.480859255056105	0.0
16	1.7956512917017566E-4	0.0	0.0	9.943957723185989	0.0
17	1.7956512917017566E-4	0.0	0.0	10.452665734125096	0.0
18	1.7956512917017566E-4	0.0	0.0	10.971429392297733	0.0
19	1.7956512917017566E-4	0.0	0.0	11.589492566901479	0.0
20	1.7956512917017566E-4	0.0	0.0	11.98992280495097	0.0
21	1.7956512917017566E-4	0.0	0.0	12.397176517908928	0.0
22	1.7956512917017566E-4	0.0	0.0	12.872844545080724	0.0
23	1.7956512917017566E-4	0.0	0.0	13.282253039588724	0.0
24	1.7956512917017566E-4	0.0	0.0	13.634200692762269	0.0
25	1.7956512917017566E-4	0.0	0.0	13.954724448331032	0.0
26	1.7956512917017566E-4	0.0	0.0	14.232870833415634	0.0
27	1.7956512917017566E-4	0.0	0.0	14.553753719242739	0.0
28	1.7956512917017566E-4	0.0	0.0	14.86206704602793	0.0
29	1.7956512917017566E-4	0.0	0.0	15.172894284621504	0.0
30	1.7956512917017566E-4	0.0	0.0	15.503473687423798	0.0
31	1.7956512917017566E-4	0.0	0.0	15.825613529155092	0.0
32	1.7956512917017566E-4	0.0	0.0	16.157090757603235	0.0
33	1.7956512917017566E-4	0.0	0.0	16.459658000254983	0.0
34	1.7956512917017566E-4	0.0	0.0	16.78197740711545	0.0
35	1.7956512917017566E-4	0.0	0.0	17.10106464165085	0.0
36	3.591302583403513E-4	0.0	0.0	17.423204483382147	0.0
37	3.591302583403513E-4	0.0	0.0	17.73367259171738	0.0
38	3.591302583403513E-4	0.0	0.0	18.047372872377675	0.0
39	3.591302583403513E-4	0.0	0.0	18.36789662794644	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	25	3.8882063E-5	45.000004	1
ACGATCG	35	1.2104283E-7	45.000004	40
GCTAGGT	25	3.8882063E-5	45.000004	21
TATAGCG	35	1.2104283E-7	45.000004	1
ATAACGC	25	3.8882063E-5	45.000004	11
CCTATCG	25	3.8882063E-5	45.000004	40
TACGTCG	25	3.8882063E-5	45.000004	1
CGTATCT	20	7.0301513E-4	45.0	23
TACGAAC	20	7.0301513E-4	45.0	22
GTTCCGT	20	7.0301513E-4	45.0	30
TAGATCG	20	7.0301513E-4	45.0	1
TTTGCGC	20	7.0301513E-4	45.0	13
CGATCGA	90	0.0	45.0	41
TCGAATA	20	7.0301513E-4	45.0	44
CGAAGTT	20	7.0301513E-4	45.0	37
TTGTGCG	30	2.1634787E-6	44.999996	1
CGACAAA	30	2.1634787E-6	44.999996	12
TACGGCT	550	0.0	42.545456	7
TTAGCGG	65	0.0	41.53846	2
CGGCGAA	60	3.6379788E-12	41.249996	31
>>END_MODULE