SRR3551305_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551305_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	648748
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3961	0.610560649127242	No Hit
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	2077	0.3201551295726538	Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	1518	0.23398916066022557	Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	1313	0.20238983395709892	Illumina PCR Primer Index 10 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	1192	0.18373852404939978	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1049	0.16169606688575533	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1026	0.1581507765727216	No Hit
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	958	0.14766904869070888	Illumina PCR Primer Index 10 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	958	0.14766904869070888	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	759	0.11699458033011279	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	718	0.11067471498948744	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	701	0.10805428301898426	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGCGA	20	7.031012E-4	45.0	23
GCGAATC	25	3.88892E-5	45.0	24
TTATGCG	25	3.88892E-5	45.0	30
CACGCGT	20	7.031012E-4	45.0	26
TTACTAG	25	3.88892E-5	45.0	1
GCCGATT	25	3.88892E-5	45.0	9
AACGCAC	20	7.031012E-4	45.0	37
CGAGCAT	20	7.031012E-4	45.0	4
TATAGCG	40	6.8066583E-9	45.0	1
TTACGAG	25	3.88892E-5	45.0	1
ACGTACG	20	7.031012E-4	45.0	1
CGCAACT	20	7.031012E-4	45.0	41
CGTTTCG	20	7.031012E-4	45.0	21
ATTTCGA	25	3.88892E-5	45.0	41
ACGCGTG	20	7.031012E-4	45.0	27
GTCATAC	65	0.0	41.538464	39
CACGCCA	65	0.0	41.538464	27
CGTCATA	65	0.0	41.538464	38
CTCGTCC	60	3.6379788E-12	41.249996	37
CACAACG	60	3.6379788E-12	41.249996	12