##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551305_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	648748
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.597648085234944	31.0	31.0	34.0	30.0	34.0
2	31.741970688156265	33.0	31.0	34.0	30.0	34.0
3	31.679465678506908	33.0	31.0	34.0	30.0	34.0
4	35.39975152139198	37.0	35.0	37.0	33.0	37.0
5	33.35396024342272	37.0	35.0	37.0	30.0	37.0
6	34.29104367181093	37.0	35.0	37.0	30.0	37.0
7	35.382418134622384	37.0	35.0	37.0	32.0	37.0
8	35.23430207106612	37.0	35.0	37.0	32.0	37.0
9	37.12936918495317	39.0	37.0	39.0	34.0	39.0
10	36.663994339866946	39.0	35.0	39.0	32.0	39.0
11	36.760184231781835	39.0	37.0	39.0	33.0	39.0
12	36.73344349423813	39.0	35.0	39.0	33.0	39.0
13	36.8240703632227	39.0	37.0	39.0	33.0	39.0
14	37.81667920363531	40.0	37.0	41.0	33.0	41.0
15	37.92858243878979	40.0	37.0	41.0	33.0	41.0
16	37.95921528852497	40.0	37.0	41.0	33.0	41.0
17	37.87165278351532	40.0	37.0	41.0	33.0	41.0
18	37.83923033288735	39.0	36.0	41.0	33.0	41.0
19	37.71746964923206	39.0	36.0	41.0	33.0	41.0
20	37.91016696775944	39.0	36.0	41.0	34.0	41.0
21	37.759842034195096	39.0	36.0	41.0	33.0	41.0
22	37.918970077749755	40.0	36.0	41.0	34.0	41.0
23	38.02430681867227	40.0	36.0	41.0	34.0	41.0
24	37.867313656458286	40.0	36.0	41.0	34.0	41.0
25	37.543016702941664	39.0	35.0	41.0	33.0	41.0
26	37.676197845696635	39.0	36.0	41.0	33.0	41.0
27	37.314089600276226	39.0	35.0	41.0	33.0	41.0
28	37.379655891039356	39.0	35.0	41.0	33.0	41.0
29	37.61795797443691	40.0	36.0	41.0	33.0	41.0
30	37.32820293858324	39.0	35.0	41.0	33.0	41.0
31	37.263851295109966	39.0	35.0	41.0	33.0	41.0
32	37.380198474600306	39.0	35.0	41.0	33.0	41.0
33	37.39262086357106	40.0	35.0	41.0	33.0	41.0
34	37.259547620956056	40.0	35.0	41.0	33.0	41.0
35	37.24407319945495	40.0	35.0	41.0	33.0	41.0
36	37.054959707004876	40.0	35.0	41.0	32.0	41.0
37	37.025758846270044	40.0	35.0	41.0	32.0	41.0
38	36.86983851973339	39.0	35.0	41.0	31.0	41.0
39	36.817368223100495	39.0	35.0	41.0	31.0	41.0
40	36.7145131853971	39.0	35.0	41.0	31.0	41.0
41	36.56420058327733	39.0	35.0	41.0	31.0	41.0
42	36.71745731778749	39.0	35.0	41.0	31.0	41.0
43	36.64165130374197	39.0	35.0	41.0	31.0	41.0
44	36.66594270810854	39.0	35.0	41.0	31.0	41.0
45	36.642087528593535	39.0	35.0	41.0	31.0	41.0
46	36.49067434504615	39.0	35.0	41.0	31.0	41.0
47	36.46411703774039	39.0	35.0	41.0	31.0	41.0
48	36.422466042284526	39.0	35.0	41.0	31.0	41.0
49	36.37560501149907	39.0	35.0	41.0	31.0	41.0
50	36.29186987859693	39.0	35.0	41.0	31.0	41.0
51	35.353266599665815	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	14.0
9	26.0
10	47.0
11	38.0
12	45.0
13	41.0
14	38.0
15	48.0
16	102.0
17	144.0
18	199.0
19	331.0
20	600.0
21	839.0
22	1301.0
23	1908.0
24	2713.0
25	4086.0
26	5601.0
27	6635.0
28	7464.0
29	8203.0
30	10051.0
31	12829.0
32	17379.0
33	25167.0
34	44901.0
35	53935.0
36	55343.0
37	80522.0
38	132184.0
39	175960.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.90332147459414	21.846387195027962	24.335335137834722	13.914956192543176
2	33.947850320925845	24.842465795655635	23.420033664843668	17.789650218574852
3	33.17867646605462	22.91059086116643	27.222434597100875	16.688298075678077
4	30.525566167448687	24.829671921917292	26.095340563670334	18.54942134696369
5	24.369863182622527	33.35347469279289	23.943811772830127	18.332850351754455
6	26.17102480470075	34.24981656976207	24.79776430909999	14.781394316437199
7	76.4554187450289	7.166419010154945	11.016912576223742	5.361249668592427
8	76.155764642049	7.184453747834291	10.96928237158342	5.690499238533298
9	69.5613705167492	9.456059980146374	14.134918335008354	6.847651168096086
10	40.463631487110554	25.96940568602909	20.33593937861851	13.231023448241844
11	30.382058981299366	25.28084865001511	25.42682212507784	18.910270243607687
12	28.243015778083326	22.721457330118934	29.260514097924002	19.77501279387374
13	24.041846757138366	25.368401906441328	30.221133629699047	20.368617706721253
14	19.307805187838728	28.628527563861468	30.03292495699409	22.030742291305717
15	19.406148458261143	26.889331450732794	34.331820676133106	19.372699414872955
16	21.734479335581767	26.385437797110743	32.13682354319396	19.743259324113524
17	21.596058870316362	26.535264848600686	28.728412264854768	23.140264016228183
18	21.870125225819578	25.936881500983432	31.14306325414491	21.04993001905208
19	24.261963042660632	26.650872141417004	28.34413362353333	20.743031192389033
20	24.961618378784983	27.320315438352026	29.053191686140075	18.66487449672292
21	23.76377268215085	27.106673161227473	29.689802511915257	19.43975164470642
22	21.585885428548526	25.775339577154767	29.03931881100211	23.599456183294592
23	22.51968406839019	25.72909666002824	29.591767527607022	22.159451743974547
24	22.283845191044904	24.307589387558806	30.442174773563853	22.96639064783244
25	21.334940531608577	27.04809879953387	28.280934970127074	23.336025698730477
26	19.909117253540664	28.058968967919746	28.472072360916716	23.559841417622867
27	20.968850771023572	28.07577056114238	28.72979955236856	22.225579115465482
28	18.973160610899765	28.30421057174743	30.314698465351725	22.407930352001085
29	20.755516780013195	25.225048863349098	30.830615277426677	23.188819079211033
30	20.702953997546043	26.0438567826028	29.829610264694455	23.423578955156703
31	21.2493911349245	28.390993112888207	27.29071997139105	23.068895780796243
32	22.879916392805836	27.32108615363747	27.05580595238829	22.743191501168404
33	21.48183886501384	27.266519511428168	28.206329730496275	23.04531189306171
34	20.07882875939502	27.597618798054096	29.011881346840372	23.31167109571051
35	21.19174163157343	25.827594073507743	28.155308378600015	24.825355916318816
36	21.666039818234506	28.24794835591015	26.265976927867214	23.820034897988123
37	21.290084901995847	28.69773779649417	28.75924087627245	21.252936425237536
38	20.609389161893372	28.46775635531824	28.186291133074786	22.736563349713602
39	21.79767798898802	25.669289153877934	30.02367637356878	22.50935648356527
40	22.34087812216762	25.469519751891333	29.551536189706944	22.6380659362341
41	20.037210133981144	26.022276754610417	27.9570804071843	25.983432704224136
42	21.009390395037826	25.522699106586842	27.858891279818977	25.609019218556355
43	21.237059690357427	25.257727191451846	28.64255458205652	24.862658536134216
44	20.115514806982063	26.029367335236486	28.53126329483867	25.323854562942778
45	21.52792763908328	25.871062415606676	27.499275527631685	25.101734417678358
46	21.006615820010236	26.987983007269385	27.851029983907466	24.154371188812913
47	20.38942701942819	25.81017590805675	29.984986466239587	23.81541060627547
48	19.82171814017153	24.824893487147552	30.48579725871987	24.867591113961048
49	20.178713460388316	24.843082367883987	30.911232096283918	24.066972075443775
50	19.775475223045003	24.820577481549076	30.374043542330764	25.029903753075157
51	19.839907020907965	24.6178793614778	27.929334656908384	27.61287896070585
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	351.0
1	931.5
2	1512.0
3	4203.0
4	6894.0
5	4610.0
6	2326.0
7	2183.0
8	2040.0
9	2054.0
10	2068.0
11	2140.0
12	2212.0
13	2193.0
14	2174.0
15	2191.0
16	2208.0
17	2182.5
18	2157.0
19	2256.0
20	2355.0
21	3530.5
22	4706.0
23	4387.5
24	4069.0
25	4447.0
26	6108.5
27	7392.0
28	9016.0
29	10640.0
30	11781.5
31	12923.0
32	14284.5
33	15646.0
34	16873.5
35	18101.0
36	19335.5
37	20570.0
38	21777.5
39	22985.0
40	26395.5
41	29806.0
42	32279.5
43	34753.0
44	38468.5
45	42184.0
46	46110.5
47	50037.0
48	55660.5
49	61284.0
50	58926.0
51	56568.0
52	50055.0
53	43542.0
54	38372.0
55	33202.0
56	31609.5
57	30017.0
58	28004.5
59	25992.0
60	25647.0
61	25302.0
62	23300.5
63	21299.0
64	18040.0
65	14781.0
66	12672.5
67	10564.0
68	9376.0
69	8188.0
70	6765.5
71	5343.0
72	4423.5
73	3504.0
74	2584.5
75	1439.0
76	1213.0
77	1044.0
78	875.0
79	598.0
80	321.0
81	180.0
82	39.0
83	48.5
84	58.0
85	41.0
86	24.0
87	15.5
88	7.0
89	12.0
90	17.0
91	12.0
92	7.0
93	4.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	648748.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.87877502482152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.03898428513601	21.09276395211917
2	11.183540047826963	6.4593387404455305
3	4.318134574367558	3.7410731070019234
4	2.0395086747295044	2.355940487147409
5	1.1958691571786397	1.7267618174642427
6	0.7846513451167554	1.3595861801130216
7	0.5472106196988442	1.106194066423326
8	0.38019414591370954	0.8783632964476946
9	0.3274074769687178	0.8509614181941629
>10	5.298805559500926	39.77696219288308
>50	0.8155294366805631	14.563425370921859
>100	0.06260848090995472	3.3762332847383516
>500	0.0037780979859455437	0.8207951838526456
>1k	0.0037780979859455437	1.891600902247571
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3961	0.610560649127242	No Hit
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	2077	0.3201551295726538	Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	1518	0.23398916066022557	Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	1313	0.20238983395709892	Illumina PCR Primer Index 10 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	1192	0.18373852404939978	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1049	0.16169606688575533	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	1026	0.1581507765727216	No Hit
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	958	0.14766904869070888	Illumina PCR Primer Index 10 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	958	0.14766904869070888	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	759	0.11699458033011279	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	718	0.11067471498948744	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	701	0.10805428301898426	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5414305708842262E-4	0.0	0.0	0.21133013126822742	0.0
2	1.5414305708842262E-4	0.0	0.0	1.067594813394415	0.0
3	1.5414305708842262E-4	0.0	0.0	1.4130294043295701	0.0
4	1.5414305708842262E-4	0.0	0.0	1.840468101635766	0.0
5	1.5414305708842262E-4	0.0	0.0	3.0839401431680713	0.0
6	1.5414305708842262E-4	0.0	0.0	3.8298383964189484	0.0
7	1.5414305708842262E-4	0.0	0.0	4.621671280682175	0.0
8	1.5414305708842262E-4	0.0	0.0	5.690036809362033	0.0
9	1.5414305708842262E-4	0.0	0.0	6.085722036908013	0.0
10	1.5414305708842262E-4	0.0	0.0	6.984067773619341	0.0
11	1.5414305708842262E-4	0.0	0.0	8.456288111870865	0.0
12	1.5414305708842262E-4	0.0	0.0	9.525270212779075	0.0
13	1.5414305708842262E-4	0.0	0.0	9.985387238188018	0.0
14	1.5414305708842262E-4	0.0	0.0	10.178066059548545	0.0
15	1.5414305708842262E-4	0.0	0.0	10.559107696671127	0.0
16	1.5414305708842262E-4	0.0	0.0	11.250901736883968	0.0
17	1.5414305708842262E-4	0.0	0.0	12.040114189176691	0.0
18	1.5414305708842262E-4	0.0	0.0	12.936764352260045	0.0
19	1.5414305708842262E-4	0.0	0.0	13.506168805144679	0.0
20	1.5414305708842262E-4	0.0	0.0	14.050756225838075	0.0
21	1.5414305708842262E-4	0.0	0.0	14.734072397911053	0.0
22	1.5414305708842262E-4	0.0	0.0	15.408139986558725	0.0
23	1.5414305708842262E-4	0.0	0.0	16.06047340415693	0.0
24	1.5414305708842262E-4	0.0	0.0	16.602748678994	0.0
25	1.5414305708842262E-4	0.0	0.0	17.10232632701758	0.0
26	1.5414305708842262E-4	0.0	0.0	17.548108048117296	0.0
27	1.5414305708842262E-4	0.0	0.0	18.007762644354973	0.0
28	1.5414305708842262E-4	0.0	0.0	18.472966390647834	0.0
29	1.5414305708842262E-4	0.0	0.0	18.939557424454488	0.0
30	1.5414305708842262E-4	0.0	0.0	19.459944385185004	0.0
31	1.5414305708842262E-4	0.0	0.0	19.96645847077756	0.0
32	1.5414305708842262E-4	0.0	0.0	20.46156597014557	0.0
33	1.5414305708842262E-4	0.0	0.0	20.917983562184393	0.0
34	1.5414305708842262E-4	0.0	0.0	21.373784581994858	0.0
35	1.5414305708842262E-4	0.0	0.0	21.854865063167825	0.0
36	1.5414305708842262E-4	0.0	0.0	22.31559866080512	0.0
37	3.0828611417684523E-4	0.0	0.0	22.76523395833205	0.0
38	3.0828611417684523E-4	0.0	0.0	23.229204560168203	0.0
39	4.6242917126526785E-4	0.0	0.0	23.687163582777906	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGCGA	20	7.031012E-4	45.0	23
GCGAATC	25	3.88892E-5	45.0	24
TTATGCG	25	3.88892E-5	45.0	30
CACGCGT	20	7.031012E-4	45.0	26
TTACTAG	25	3.88892E-5	45.0	1
GCCGATT	25	3.88892E-5	45.0	9
AACGCAC	20	7.031012E-4	45.0	37
CGAGCAT	20	7.031012E-4	45.0	4
TATAGCG	40	6.8066583E-9	45.0	1
TTACGAG	25	3.88892E-5	45.0	1
ACGTACG	20	7.031012E-4	45.0	1
CGCAACT	20	7.031012E-4	45.0	41
CGTTTCG	20	7.031012E-4	45.0	21
ATTTCGA	25	3.88892E-5	45.0	41
ACGCGTG	20	7.031012E-4	45.0	27
GTCATAC	65	0.0	41.538464	39
CACGCCA	65	0.0	41.538464	27
CGTCATA	65	0.0	41.538464	38
CTCGTCC	60	3.6379788E-12	41.249996	37
CACAACG	60	3.6379788E-12	41.249996	12
>>END_MODULE