##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551304_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	520588
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5897427524261	31.0	31.0	34.0	30.0	34.0
2	31.739484966998855	33.0	31.0	34.0	30.0	34.0
3	31.6789591769307	33.0	31.0	34.0	30.0	34.0
4	35.35044219229026	37.0	35.0	37.0	33.0	37.0
5	33.402804136860624	37.0	35.0	37.0	30.0	37.0
6	34.29930194318732	37.0	35.0	37.0	30.0	37.0
7	35.3530757528026	37.0	35.0	37.0	32.0	37.0
8	35.19766302719233	37.0	35.0	37.0	32.0	37.0
9	37.08736659316004	39.0	37.0	39.0	34.0	39.0
10	36.65810967598178	38.0	35.0	39.0	32.0	39.0
11	36.77516961589587	39.0	37.0	39.0	33.0	39.0
12	36.752583616986946	39.0	35.0	39.0	33.0	39.0
13	36.82348229309934	39.0	37.0	39.0	33.0	39.0
14	37.81651132949665	40.0	37.0	41.0	33.0	41.0
15	37.93818528279561	40.0	37.0	41.0	33.0	41.0
16	38.00132350342305	40.0	37.0	41.0	33.0	41.0
17	37.87749045310303	40.0	37.0	41.0	33.0	41.0
18	37.839239475362476	39.0	37.0	41.0	33.0	41.0
19	37.72711241903386	39.0	36.0	41.0	33.0	41.0
20	37.89292876516554	39.0	36.0	41.0	34.0	41.0
21	37.758847687614775	39.0	36.0	41.0	33.0	41.0
22	37.90058357088523	40.0	36.0	41.0	33.0	41.0
23	37.96925591830776	40.0	36.0	41.0	34.0	41.0
24	37.82593720946315	40.0	36.0	41.0	33.0	41.0
25	37.50547265784075	39.0	35.0	41.0	33.0	41.0
26	37.583230885076105	39.0	35.0	41.0	33.0	41.0
27	37.22994767455262	39.0	35.0	41.0	33.0	41.0
28	37.27799142508087	39.0	35.0	41.0	33.0	41.0
29	37.47844552698103	40.0	35.0	41.0	33.0	41.0
30	37.20748461355237	39.0	35.0	41.0	32.0	41.0
31	37.149021875264125	39.0	35.0	41.0	32.0	41.0
32	37.224822700484836	39.0	35.0	41.0	33.0	41.0
33	37.19597839366255	40.0	35.0	41.0	33.0	41.0
34	37.054872567174044	40.0	35.0	41.0	32.0	41.0
35	37.022670518721135	40.0	35.0	41.0	32.0	41.0
36	36.811997203162576	39.0	35.0	41.0	31.0	41.0
37	36.80610194626077	39.0	35.0	41.0	31.0	41.0
38	36.650504813787485	39.0	35.0	41.0	31.0	41.0
39	36.59960659869225	39.0	35.0	41.0	31.0	41.0
40	36.50620644348314	39.0	35.0	41.0	31.0	41.0
41	36.30008375145028	39.0	35.0	41.0	30.0	41.0
42	36.49107931800195	39.0	35.0	41.0	31.0	41.0
43	36.41266029950748	39.0	35.0	41.0	31.0	41.0
44	36.475979085188285	39.0	35.0	41.0	31.0	41.0
45	36.481061799349966	39.0	35.0	41.0	31.0	41.0
46	36.357774285999675	39.0	35.0	41.0	31.0	41.0
47	36.2916029566567	39.0	35.0	41.0	31.0	41.0
48	36.27701176362114	39.0	35.0	41.0	31.0	41.0
49	36.230539313238104	39.0	35.0	41.0	31.0	41.0
50	36.13941158843462	39.0	35.0	41.0	30.0	41.0
51	35.21326269525998	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	19.0
9	30.0
10	38.0
11	37.0
12	36.0
13	32.0
14	38.0
15	39.0
16	67.0
17	121.0
18	177.0
19	317.0
20	576.0
21	947.0
22	1359.0
23	2095.0
24	2926.0
25	3905.0
26	5084.0
27	5852.0
28	6245.0
29	6947.0
30	8194.0
31	10191.0
32	13759.0
33	19965.0
34	34917.0
35	42785.0
36	44099.0
37	62842.0
38	105164.0
39	141751.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.30480917731488	21.900235887112267	23.49650779503177	14.298447140541082
2	33.49808293698664	25.325017096052925	23.818643533850185	17.358256433110252
3	31.979607674398945	24.25411265722606	27.726724396259616	16.039555272115376
4	28.61610332931224	24.353231346093263	28.601504452657377	18.429160871937118
5	24.95389828424781	32.75584531337641	25.009028252668138	17.28122814970764
6	24.98290394707523	35.01559774716282	25.55494940336696	14.446548902394984
7	73.78982996150508	7.192828109752818	13.800164429452849	5.217177499289265
8	73.66112933836354	8.298693016358426	12.314920820303197	5.725256824974837
9	67.92184990818075	8.8075407039732	15.135961643372495	8.134647744473558
10	39.77156599844791	25.59221495693331	21.089998232767563	13.546220811851214
11	30.82917777589956	25.37476853096883	25.690757374353616	18.105296318777995
12	29.143199612745583	21.605376996780564	29.65223939084266	19.599183999631187
13	25.00998870507964	24.43333307721269	30.175685955112296	20.380992262595374
14	18.726132757574128	29.152227865413728	29.83991179205053	22.28172758496162
15	17.834832919698496	27.154871030450185	35.548264654582894	19.46203139526843
16	20.077105119595533	25.102960498513223	33.6588626706724	21.161071711218852
17	19.69081115968866	26.72285953575572	29.769990856493045	23.816338448062577
18	19.929963810153133	26.77933413755215	31.212014107125018	22.07868794516969
19	22.354145696789015	26.66177476238407	29.182386071134946	21.801693469691962
20	25.497706439641327	26.14601181740647	30.055245222709704	18.301036520242494
21	23.533581258115824	27.48930056013585	29.351425695559634	19.625692486188694
22	20.24518429160872	26.79873527626453	29.411934197484385	23.544146234642366
23	21.849523999784857	26.32254297064089	29.560804321267494	22.26712870830676
24	22.58350173265615	24.678248442146188	29.79361798581604	22.94463183938162
25	20.56674375898023	27.697910823914494	27.157752387684695	24.577593029420576
26	19.177161210016365	29.857007844975296	28.78283786794932	22.182993077059017
27	22.715467893996788	28.900205152635095	28.435730366431805	19.94859658693631
28	18.8171836461847	27.833526704418848	30.67454493764743	22.674744711749025
29	20.428054430759065	27.1101139480741	30.553335843315637	21.9084957778512
30	22.073693592629873	28.40787724649819	27.989504176047085	21.52892498482485
31	20.90866481747562	27.845052133356894	26.161186965508232	25.08509608365925
32	22.513388706616364	28.794363296887365	26.41532267359217	22.276925322904102
33	22.66187464943487	28.842770098427163	26.139672831490547	22.355682420647423
34	20.250754915595444	27.565560481609257	27.359063213135915	24.824621389659384
35	21.711410943010595	26.761469722698177	26.79508555710082	24.732033777190406
36	21.185659292953353	30.68703081899698	25.212452073424668	22.914857814625
37	21.272484190953307	28.020814924662112	28.36926705955573	22.33743382482885
38	19.964732187449577	27.797797874710906	26.87730028352555	25.360169654313967
39	21.21677795108608	27.05728906544139	28.549255841471567	23.17667714200097
40	21.95536585553259	26.07205698172067	28.76055537200243	23.212021790744313
41	18.909963349135978	27.36021575602972	27.16543600697673	26.564384887857578
42	20.207534557077768	27.69445319523308	27.975673661321427	24.12233858636772
43	22.37008920681998	26.789130752149493	27.829876985255137	23.01090305577539
44	21.193535002727685	26.462576932238164	27.259560343304113	25.084327721730045
45	20.74846135523677	26.053616295419797	26.701537492220336	26.4963848571231
46	21.53315097543547	27.935526750520566	26.734577055176068	23.796745218867894
47	19.343703658171144	26.721514902379617	30.37008152320069	23.56469991624855
48	19.89231407562218	25.338079248849375	29.1714369136438	25.598169761884638
49	20.49067592798912	25.259322151106055	30.514725656373177	23.735276264531645
50	19.12145497014914	25.119288189508787	29.4645669896348	26.294689850707275
51	18.78107063551215	25.183638501079546	27.052486803383868	28.982804060024435
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	404.0
1	915.5
2	1427.0
3	4080.0
4	6733.0
5	4544.0
6	2355.0
7	2302.5
8	2250.0
9	2269.0
10	2288.0
11	2178.5
12	2069.0
13	2055.5
14	2042.0
15	1998.5
16	1955.0
17	2010.0
18	2065.0
19	2107.0
20	2149.0
21	2914.0
22	3679.0
23	3500.0
24	3321.0
25	4005.5
26	5347.0
27	6004.0
28	6586.5
29	7169.0
30	8485.0
31	9801.0
32	10499.5
33	11198.0
34	12279.0
35	13360.0
36	14236.0
37	15112.0
38	16956.5
39	18801.0
40	20999.5
41	23198.0
42	26048.5
43	28899.0
44	30829.5
45	32760.0
46	39723.5
47	46687.0
48	47935.5
49	49184.0
50	47037.5
51	44891.0
52	38962.5
53	33034.0
54	29303.5
55	25573.0
56	23337.5
57	21102.0
58	21401.0
59	21700.0
60	21497.0
61	21294.0
62	20266.5
63	19239.0
64	15642.5
65	12046.0
66	9489.0
67	6932.0
68	6158.0
69	5384.0
70	4752.5
71	4121.0
72	2998.0
73	1875.0
74	1759.5
75	1329.0
76	1014.0
77	793.5
78	573.0
79	462.0
80	351.0
81	227.5
82	104.0
83	74.5
84	45.0
85	51.0
86	57.0
87	31.5
88	6.0
89	3.0
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	520588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.10182712184612
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.88328151364844	22.979050488321835
2	10.956286089310153	6.815210316944237
3	4.0737920954413624	3.801071324482816
4	1.8862804067306953	2.3466706845385468
5	1.093860708353171	1.7010533323290233
6	0.6987457968970423	1.3039362586325047
7	0.5360136601079668	1.166970293413818
8	0.43319147648754674	1.0778437129898357
9	0.354504806773738	0.9923172492726238
>10	5.618127533752281	42.77163009173515
>50	0.3965906225683438	7.453068267688084
>100	0.05870790318334537	3.323179960999967
>500	0.005620969453724558	1.1413961568176179
>1k	0.004996417292199607	3.126601861833965
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3860	0.7414692616810222	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	2626	0.5044296065218561	No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT	1850	0.3553673922564485	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC	1707	0.32789845328743655	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCC	1704	0.32732218184053413	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCG	1565	0.30062160480072536	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC	1557	0.29908488094231905	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	1227	0.23569502178306068	No Hit
TCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	926	0.17787578661052503	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	883	0.16961589587159137	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	723	0.1388814187034661	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	650	0.12485881349550894	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC	576	0.11064411780525099	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	557	0.10699439864153612	No Hit
ACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	540	0.1037288604424228	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9209048230078296E-4	0.0	0.0	0.3690058164998041	0.0
2	1.9209048230078296E-4	0.0	0.0	1.4278085549417197	0.0
3	1.9209048230078296E-4	0.0	0.0	1.8402268204415007	0.0
4	1.9209048230078296E-4	0.0	0.0	2.408430467087217	0.0
5	1.9209048230078296E-4	0.0	0.0	3.9749283502501016	0.0
6	1.9209048230078296E-4	0.0	0.0	4.830115177453187	0.0
7	1.9209048230078296E-4	0.0	0.0	5.671471489930617	0.0
8	1.9209048230078296E-4	0.0	0.0	6.946952292407816	0.0
9	1.9209048230078296E-4	0.0	0.0	7.383766049159796	0.0
10	1.9209048230078296E-4	0.0	0.0	8.735314682628106	0.0
11	1.9209048230078296E-4	0.0	0.0	10.523484982366094	0.0
12	1.9209048230078296E-4	0.0	0.0	12.119372709320999	0.0
13	1.9209048230078296E-4	0.0	0.0	12.721576371333953	0.0
14	1.9209048230078296E-4	0.0	0.0	13.001452204046194	0.0
15	1.9209048230078296E-4	0.0	0.0	13.479373324010542	0.0
16	1.9209048230078296E-4	0.0	0.0	14.26406294420924	0.0
17	1.9209048230078296E-4	0.0	0.0	15.130198928903472	0.0
18	1.9209048230078296E-4	0.0	0.0	16.05012024864192	0.0
19	1.9209048230078296E-4	0.0	0.0	16.699386078818566	0.0
20	1.9209048230078296E-4	0.0	0.0	17.28410950694215	0.0
21	1.9209048230078296E-4	0.0	0.0	17.970832981167447	0.0
22	1.9209048230078296E-4	0.0	0.0	18.70423444259184	0.0
23	1.9209048230078296E-4	0.0	0.0	19.36733078749414	0.0
24	1.9209048230078296E-4	0.0	0.0	19.912483576263764	0.0
25	1.9209048230078296E-4	0.0	0.0	20.39712786310864	0.0
26	1.9209048230078296E-4	0.0	0.0	20.87408853066148	0.0
27	1.9209048230078296E-4	0.0	0.0	21.325501164068324	0.0
28	1.9209048230078296E-4	0.0	0.0	21.77345616879375	0.0
29	1.9209048230078296E-4	0.0	0.0	22.23754677403244	0.0
30	1.9209048230078296E-4	0.0	0.0	22.721806879912712	0.0
31	1.9209048230078296E-4	0.0	0.0	23.229502024633682	0.0
32	1.9209048230078296E-4	0.0	0.0	23.730089821509523	0.0
33	1.9209048230078296E-4	0.0	0.0	24.16075668282788	0.0
34	1.9209048230078296E-4	0.0	0.0	24.63483599314621	0.0
35	1.9209048230078296E-4	0.0	0.0	25.077220373884916	0.0
36	1.9209048230078296E-4	0.0	0.0	25.555909855778467	0.0
37	1.9209048230078296E-4	0.0	0.0	26.025378994521578	0.0
38	1.9209048230078296E-4	0.0	0.0	26.450667322335512	0.0
39	1.9209048230078296E-4	0.0	0.0	26.899966960437045	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGTA	30	2.163204E-6	45.000004	8
AACGGGC	30	2.163204E-6	45.000004	4
GTCGTAC	30	2.163204E-6	45.000004	9
TCACGGT	20	7.029726E-4	45.0	18
CGGGTAT	95	0.0	45.0	6
CACGGTC	20	7.029726E-4	45.0	19
ACGCATG	25	3.8878556E-5	45.0	1
CCGGCAT	20	7.029726E-4	45.0	36
CGTTGTA	20	7.029726E-4	45.0	19
CGCGCCT	35	1.21021E-7	45.0	17
ATTCGAC	20	7.029726E-4	45.0	30
TAACGCC	20	7.029726E-4	45.0	12
ACTTACG	45	3.8380676E-10	45.0	1
TTATCGC	35	1.21021E-7	45.0	31
ACGGGTA	55	1.8189894E-12	45.0	5
TATTGCG	35	1.21021E-7	45.0	1
ATCCGAC	20	7.029726E-4	45.0	15
ACGATTG	20	7.029726E-4	45.0	1
CTAACGA	20	7.029726E-4	45.0	20
TCTACGG	25	3.8878556E-5	45.0	2
>>END_MODULE