##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551303_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	217161
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.503833561274813	31.0	31.0	34.0	30.0	34.0
2	31.658014100137684	31.0	31.0	34.0	30.0	34.0
3	31.59607388066918	31.0	31.0	34.0	30.0	34.0
4	35.298336257431124	37.0	35.0	37.0	33.0	37.0
5	33.31775963455685	37.0	35.0	37.0	30.0	37.0
6	34.25212169772657	37.0	35.0	37.0	30.0	37.0
7	35.31419085379051	37.0	35.0	37.0	32.0	37.0
8	35.20542823066757	37.0	35.0	37.0	32.0	37.0
9	37.005802146794316	39.0	37.0	39.0	33.0	39.0
10	36.608953725576875	38.0	35.0	39.0	32.0	39.0
11	36.6650503543454	39.0	35.0	39.0	32.0	39.0
12	36.48584230133403	39.0	35.0	39.0	32.0	39.0
13	36.42721298944102	39.0	35.0	39.0	32.0	39.0
14	37.333605021159414	39.0	36.0	41.0	32.0	41.0
15	37.531039182910376	39.0	36.0	41.0	33.0	41.0
16	37.65855287091144	39.0	36.0	41.0	33.0	41.0
17	37.518348138017416	39.0	36.0	41.0	33.0	41.0
18	37.50671621515834	39.0	36.0	40.0	33.0	41.0
19	37.31651171250823	39.0	36.0	40.0	33.0	41.0
20	37.379386722293596	39.0	35.0	41.0	33.0	41.0
21	37.24816150229553	39.0	35.0	40.0	33.0	41.0
22	37.40760081230055	39.0	35.0	41.0	33.0	41.0
23	37.4780600568242	39.0	35.0	41.0	33.0	41.0
24	37.33744088487344	39.0	35.0	41.0	33.0	41.0
25	37.063169722003494	39.0	35.0	40.0	33.0	41.0
26	37.14973683119897	39.0	35.0	41.0	33.0	41.0
27	36.74921832189021	39.0	35.0	41.0	32.0	41.0
28	36.78573500766712	39.0	35.0	41.0	32.0	41.0
29	37.008136820147264	39.0	35.0	41.0	32.0	41.0
30	36.725355841978995	39.0	35.0	41.0	31.0	41.0
31	36.63618237160448	39.0	35.0	40.0	31.0	41.0
32	36.5992926906765	39.0	35.0	40.0	31.0	41.0
33	36.46796155847505	39.0	35.0	41.0	31.0	41.0
34	36.2569199810279	39.0	35.0	41.0	30.0	41.0
35	36.22323529547202	39.0	35.0	41.0	30.0	41.0
36	35.975419159057104	39.0	35.0	41.0	30.0	41.0
37	35.97814985195316	39.0	35.0	41.0	30.0	41.0
38	35.83882004595669	38.0	35.0	40.0	29.0	41.0
39	35.80576162386432	38.0	35.0	40.0	29.0	41.0
40	35.73060540336432	38.0	35.0	40.0	29.0	41.0
41	35.5246982653423	38.0	35.0	40.0	28.0	41.0
42	35.662678841965175	38.0	35.0	40.0	29.0	41.0
43	35.55997623882742	38.0	35.0	40.0	28.0	41.0
44	35.65852063676259	38.0	35.0	40.0	29.0	41.0
45	35.65336317294542	38.0	35.0	40.0	29.0	41.0
46	35.524260801893526	38.0	35.0	40.0	28.0	41.0
47	35.411464305285016	38.0	35.0	40.0	27.0	41.0
48	35.43472815100317	38.0	35.0	40.0	28.0	41.0
49	35.437914726861635	38.0	35.0	40.0	28.0	41.0
50	35.314071126951895	38.0	35.0	40.0	27.0	41.0
51	34.48814013565972	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	8.0
10	11.0
11	18.0
12	12.0
13	10.0
14	9.0
15	22.0
16	26.0
17	49.0
18	114.0
19	171.0
20	320.0
21	521.0
22	874.0
23	1240.0
24	1683.0
25	2318.0
26	2913.0
27	3238.0
28	3264.0
29	3213.0
30	3944.0
31	5028.0
32	6614.0
33	9544.0
34	17773.0
35	21127.0
36	17777.0
37	24733.0
38	40225.0
39	50346.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.398676557945485	19.451927371857746	22.138873923034062	15.010522147162705
2	34.87136272166733	25.26374441082883	24.099170661398688	15.76572220610515
3	28.829762250127782	26.801313311322016	29.506679376131075	14.862245062419127
4	26.088017645894062	22.798752998927064	33.67962018962889	17.433609165549985
5	24.542620452106963	31.732677598648007	26.942222590612495	16.782479358632536
6	23.589871109453355	33.50647676148111	28.259678303194402	14.643973825871129
7	71.47277826129002	6.008905834841431	17.04173401301339	5.476581890855172
8	71.16194896873748	8.510736274008684	14.082639147913302	6.244675609340535
9	66.18269394596635	7.538185954199879	16.052606130935114	10.22651396889865
10	42.79543748647317	21.47899484714106	21.564185097692494	14.161382568693273
11	33.13670502530381	22.211631001883394	27.08773674831116	17.563927224501636
12	27.970491939160347	20.115490350477298	31.8441156561261	20.06990205423626
13	23.292395964284562	26.26714741597248	34.04110314467147	16.39935347507149
14	17.607213081538582	30.375619931755704	33.05842209236465	18.958744894341066
15	16.602889100713295	23.48534036958754	41.597708612504086	18.314061917195076
16	18.60509023259241	22.775268119045318	36.66173944676991	21.957902201592365
17	17.8135116342253	23.641906235465854	32.993953794650054	25.5506283356588
18	20.295541096237354	23.603685744677914	35.0541763944723	21.04659676461243
19	21.464259236234867	26.32148498118907	30.329571147673846	21.884684634902214
20	25.281703436620756	24.749379492634496	31.8514834615792	18.117433609165552
21	20.78365820750503	27.35758262303084	33.804412394490726	18.054346774973407
22	19.866366428594453	23.559939399800147	31.83030102090154	24.743393150703856
23	19.730062027712158	26.228926925184542	31.541575144708307	22.499435902395
24	20.781355768300937	23.870308204511858	33.02342501646244	22.32491101072476
25	18.2030843475578	26.874530878012166	31.390535132919815	23.531849641510217
26	17.852192612854058	27.390277259728958	32.234148857299424	22.52338127011756
27	19.456992738106752	27.970491939160347	30.900576070288864	21.67193925244404
28	16.740114477277228	25.266046850032925	36.10777257426518	21.88606609842467
29	21.01804651848168	22.364052477194342	34.54349537900452	22.074405625319464
30	20.48894598938115	24.680306316511714	31.787936139546236	23.042811554560902
31	22.77711007040859	24.29948287215476	30.507779942070627	22.415627115366018
32	25.513789308393314	25.02705366064809	28.698062727653678	20.761094303304922
33	21.849687559000007	23.40153158255856	29.76363159130783	24.985149267133604
34	21.067318717449265	22.836052514033366	33.79105824710699	22.30557052141038
35	22.02052854794369	23.673219408641515	29.703768172001414	24.602483871413376
36	23.42363499891785	23.07182228853247	32.20329617196458	21.301246540585094
37	20.269753777151514	23.967471138924576	35.016416391525176	20.74635869239873
38	19.49751566809878	25.637660537573502	30.685067760785778	24.179756033541935
39	21.016665054959223	23.155631075561452	33.65337238270224	22.174331486777092
40	22.784017388020867	21.885145122743033	31.678800521272237	23.65203696796386
41	20.537757700507917	22.443256385815133	31.19022292216374	25.82876299151321
42	23.455408659934335	21.218358729237753	32.71259572390991	22.613636886918002
43	21.752985112428107	22.156372460985168	31.855167364305746	24.23547506228098
44	19.631978117617805	24.25067116102799	31.425071720981208	24.692279000372995
45	21.1078416474413	22.290834910504188	30.945243390848265	25.65608005120625
46	23.637761844898485	23.929250648136634	31.347709763723692	21.08527774324119
47	18.490428760228586	22.86368178448248	35.52986033403788	23.116029121251053
48	20.647814294463554	21.634639737337736	32.77844548514696	24.939100483051746
49	21.773246577424125	19.239642477240388	35.26323787420394	23.723873071131557
50	19.928532287104957	22.14578124064634	33.05980355588711	24.865882916361592
51	20.01418302549721	21.894815367400223	30.202476503607922	27.888525103494644
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	214.0
1	301.0
2	388.0
3	2068.0
4	3748.0
5	2487.5
6	1227.0
7	1217.5
8	1208.0
9	1201.5
10	1195.0
11	1171.5
12	1148.0
13	1151.0
14	1154.0
15	1108.0
16	1062.0
17	1067.5
18	1073.0
19	1002.5
20	932.0
21	964.5
22	997.0
23	1109.5
24	1222.0
25	1394.0
26	1719.0
27	1872.0
28	2225.5
29	2579.0
30	3091.5
31	3604.0
32	3567.0
33	3530.0
34	3910.0
35	4290.0
36	4911.0
37	5532.0
38	5978.5
39	6425.0
40	8057.0
41	9689.0
42	11613.5
43	13538.0
44	15045.5
45	16553.0
46	20943.0
47	25333.0
48	24431.0
49	23529.0
50	22505.0
51	21481.0
52	17830.5
53	14180.0
54	12154.5
55	10129.0
56	8929.5
57	7730.0
58	7501.5
59	7273.0
60	6805.5
61	6338.0
62	5815.0
63	5292.0
64	4652.5
65	4013.0
66	3124.5
67	2236.0
68	1877.0
69	1518.0
70	1409.0
71	1300.0
72	1187.0
73	1074.0
74	682.0
75	284.5
76	279.0
77	275.5
78	272.0
79	170.0
80	68.0
81	70.5
82	73.0
83	38.5
84	4.0
85	2.5
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	217161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.80661352636984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.64548985313408	27.44415433710473
2	10.176444867409142	7.287680568794581
3	3.5623344221816406	3.82665395720226
4	1.74901617839965	2.5050538540529836
5	0.9876797242727436	1.7682733087432827
6	0.6378764885928135	1.370411814276044
7	0.5015561099822526	1.2571318054346774
8	0.3382288639111088	0.968866417082257
9	0.3099359551428792	0.9987981267354635
>10	4.843231564597855	41.10913101339559
>50	0.20833869183878184	4.5938267000059865
>100	0.02957895007587644	2.132979678671585
>500	0.003858123922940405	0.8721639705103587
>1k	0.0064302065382340085	3.8648744479902004
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2286	1.0526752041112355	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG	1753	0.8072351849549412	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC	1540	0.7091512748605873	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC	1451	0.6681678570277352	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1363	0.6276449270357016	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	730	0.3361561237975511	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC	586	0.269845874719678	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	578	0.2661619719931295	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCT	409	0.18833952689479233	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	405	0.18649757553151808	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTA	375	0.1726829403069612	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTCTGTT	355	0.16347318349058992	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT	346	0.15932879292322286	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTC	294	0.13538342520065758	No Hit
ACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	259	0.11926635077200787	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3504312468629266	0.0
2	0.0	0.0	0.0	1.59282744139141	0.0
3	0.0	0.0	0.0	2.060222599822252	0.0
4	0.0	0.0	0.0	2.767071435478746	0.0
5	0.0	0.0	0.0	4.907879407444247	0.0
6	0.0	0.0	0.0	5.818263868742545	0.0
7	0.0	0.0	0.0	6.957971274768489	0.0
8	0.0	0.0	0.0	8.588098231266203	0.0
9	0.0	0.0	0.0	9.164629007971044	0.0
10	0.0	0.0	0.0	11.247415511993406	0.0
11	0.0	0.0	0.0	13.120219560602502	0.0
12	0.0	0.0	0.0	15.2808285097232	0.0
13	0.0	0.0	0.0	15.953601245159122	0.0
14	0.0	0.0	0.0	16.23634077942172	0.0
15	0.0	0.0	0.0	16.833133021122578	0.0
16	0.0	0.0	0.0	17.687337965841014	0.0
17	0.0	0.0	0.0	18.683833653372382	0.0
18	0.0	0.0	0.0	19.917941066766133	0.0
19	0.0	0.0	0.0	20.766620157394744	0.0
20	0.0	0.0	0.0	21.428341184651018	0.0
21	0.0	0.0	0.0	22.157753924507624	0.0
22	0.0	0.0	0.0	22.87058910209476	0.0
23	0.0	0.0	0.0	23.573293547183887	0.0
24	0.0	0.0	0.0	24.118511150713065	0.0
25	0.0	0.0	0.0	24.60110240789092	0.0
26	0.0	0.0	0.0	25.07770732313813	0.0
27	0.0	0.0	0.0	25.513328820552495	0.0
28	0.0	0.0	0.0	25.986249833073156	0.0
29	0.0	0.0	0.0	26.46930157809183	0.0
30	0.0	0.0	0.0	26.97537771515143	0.0
31	0.0	0.0	0.0	27.485137754937583	0.0
32	0.0	0.0	0.0	27.940099741666323	0.0
33	0.0	0.0	0.0	28.377563190443958	0.0
34	0.0	0.0	0.0	28.825617859560417	0.0
35	0.0	0.0	0.0	29.30866960457909	0.0
36	0.0	0.0	0.0	29.74198866278936	0.0
37	0.0	0.0	0.0	30.2291847983754	0.0
38	0.0	0.0	0.0	30.678620931014315	0.0
39	0.0	0.0	0.0	31.132201454220603	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACTCA	30	2.157345E-6	45.000004	29
TTGTGCG	35	1.2058445E-7	45.000004	1
GCGAAAG	30	2.157345E-6	45.000004	1
GACCGAT	35	1.2058445E-7	45.000004	9
GCCGGGA	30	2.157345E-6	45.000004	4
ATATGCG	30	2.157345E-6	45.000004	1
TCTACTC	30	2.157345E-6	45.000004	28
CGAGGGA	75	0.0	45.000004	4
GCGATTC	35	1.2058445E-7	45.000004	9
GCGATAT	30	2.157345E-6	45.000004	9
CGTAAGG	30	2.157345E-6	45.000004	2
CGTTTGT	30	2.157345E-6	45.000004	39
CGATATG	30	2.157345E-6	45.000004	10
TCACGGA	20	7.02064E-4	45.0	39
ACAACTT	20	7.02064E-4	45.0	26
ACAACGA	40	6.7702786E-9	45.0	13
CGGGTTC	20	7.02064E-4	45.0	6
CGGGTTA	45	3.8198777E-10	45.0	6
AACCGGA	20	7.02064E-4	45.0	30
AGGTAAG	25	3.8803257E-5	45.0	29
>>END_MODULE