FastQCFastQC Report
Sat 18 Jun 2016
SRR3551297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360223
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84092.3343873100829207No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCG50331.397190073926429No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGC46771.2983624032890737No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTC44941.2475605388884108No Hit
GCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC27020.7500909159048701No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCTCCTT14610.4055820977561122No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT12680.35200417519147864No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC12340.34256557743397836No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCT11750.32618683426655154No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTA9810.2723313058855209No Hit
GAACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT9400.26094946741324127No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTC8010.2223622589340492No Hit
TCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC7220.20043139943868105No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCC7040.19543449474353386No Hit
TGGTTAGGGAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTC6130.17017236545140094No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC5730.15906813279551832No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4250.1179824719687527No Hit
TGGTTAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCT4100.1138183847227967No Hit
TGGTTAGGGAACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGC4060.11270796145720845No Hit
ACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC3930.10909908584404661No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTG3720.10326936369970824No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGA253.8854538E-545.00000441
CTTCGAA253.8854538E-545.00000442
ACGGCCA302.161336E-645.00000430
CGGGACT502.1827873E-1145.0000046
GTATCCA253.8854538E-545.0000049
CGTAAGG302.161336E-645.0000042
CGGTCTA253.8854538E-545.00000431
TGTCGTA253.8854538E-545.0000041
TGCGTTG253.8854538E-545.0000041
TCACGAA207.026831E-445.021
GCCCCGA207.026831E-445.027
ATTTACT207.026831E-445.045
GTGCAAG453.8380676E-1045.01
TTAGCCG207.026831E-445.01
TTCACGA207.026831E-445.020
CCGTGTT207.026831E-445.030
CACGAGG207.026831E-445.02
CGTTATT1050.045.01
ATTCACG207.026831E-445.019
CGCTATA207.026831E-445.013