SRR3551296_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551296_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483362
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	14430	2.9853401798238175	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	14069	2.9106549542578852	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	13180	2.7267348281412276	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8596	1.778377282450834	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	4483	0.9274622332744403	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGAGAAT	3600	0.7447834128458588	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	3167	0.6552025190230096	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTA	2749	0.5687248894203517	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	2608	0.5395542057505555	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTC	2396	0.49569473810518827	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	1484	0.30701627351757066	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATG	791	0.16364546654474285	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	770	0.15930089663647534	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCCGAGAA	681	0.14088819559667495	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	654	0.13530232000033102	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGT	627	0.12971644440398708	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTAT	583	0.1206135360247599	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCC	553	0.11440700758437775	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	536	0.11088997480149454	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	35	1.2099736E-7	45.000004	16
CGGCGAT	230	0.0	45.000004	31
CGAGCCC	35	1.2099736E-7	45.000004	34
ACCCGTC	35	1.2099736E-7	45.000004	39
CGATCAC	70	0.0	45.000004	34
TTGTGCG	25	3.88744E-5	45.0	1
GCGAACC	55	1.8189894E-12	45.0	33
GTTTACG	20	7.029225E-4	45.0	1
TAGCGTG	25	3.88744E-5	45.0	1
GTTAGCG	20	7.029225E-4	45.0	1
ACGGGTC	25	3.88744E-5	45.0	5
ATCGTAA	25	3.88744E-5	45.0	41
CTACGTG	20	7.029225E-4	45.0	14
CGGGCAA	20	7.029225E-4	45.0	6
ACTCGTA	20	7.029225E-4	45.0	40
TACTAGG	25	3.88744E-5	45.0	2
TAGTGCG	40	6.8012014E-9	45.0	1
GTGACGC	20	7.029225E-4	45.0	9
CAATCGA	170	0.0	45.0	41
CGATCGA	130	0.0	44.999996	41

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content