##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551296_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483362
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50352530815414	31.0	31.0	34.0	30.0	34.0
2	31.672835266322135	31.0	31.0	34.0	30.0	34.0
3	31.65817337730314	31.0	31.0	34.0	30.0	34.0
4	35.4419027561124	37.0	35.0	37.0	33.0	37.0
5	34.21532722886781	37.0	35.0	37.0	32.0	37.0
6	34.66350685407624	37.0	35.0	37.0	32.0	37.0
7	35.30211932257811	37.0	35.0	37.0	32.0	37.0
8	34.68274088571299	36.0	35.0	37.0	32.0	37.0
9	36.351080556601474	39.0	35.0	39.0	32.0	39.0
10	36.32159747766684	37.0	35.0	39.0	32.0	39.0
11	36.62601321576789	39.0	35.0	39.0	32.0	39.0
12	36.638840041211346	39.0	35.0	39.0	32.0	39.0
13	36.592185566925	39.0	35.0	39.0	32.0	39.0
14	37.68227746492277	40.0	37.0	41.0	32.0	41.0
15	37.648445678394246	40.0	36.0	41.0	32.0	41.0
16	37.94270753596683	40.0	37.0	41.0	33.0	41.0
17	37.578065714723124	40.0	36.0	41.0	32.0	41.0
18	37.5008151240685	39.0	36.0	40.0	32.0	41.0
19	37.39774330625908	39.0	36.0	40.0	32.0	41.0
20	36.89603444209516	39.0	35.0	41.0	31.0	41.0
21	37.10629300606998	39.0	35.0	40.0	32.0	41.0
22	37.06365829337019	39.0	35.0	41.0	32.0	41.0
23	36.88834248451471	39.0	35.0	41.0	31.0	41.0
24	36.94950575345187	39.0	35.0	41.0	31.0	41.0
25	36.98766762798896	39.0	35.0	40.0	32.0	41.0
26	36.847292505410024	39.0	35.0	41.0	31.0	41.0
27	36.63270592226944	39.0	35.0	41.0	30.0	41.0
28	36.454421323976646	39.0	35.0	41.0	30.0	41.0
29	36.57972492665952	39.0	35.0	41.0	30.0	41.0
30	36.24388139737919	39.0	35.0	41.0	30.0	41.0
31	36.28783396295116	39.0	35.0	40.0	30.0	41.0
32	35.69411952118702	39.0	35.0	41.0	25.0	41.0
33	35.28969178379765	39.0	35.0	41.0	23.0	41.0
34	34.89722609555571	39.0	35.0	41.0	21.0	41.0
35	34.672884918549656	39.0	35.0	41.0	18.0	41.0
36	34.64199295765906	39.0	35.0	41.0	18.0	41.0
37	34.594881269110935	39.0	35.0	41.0	18.0	41.0
38	34.6387862512982	39.0	34.0	40.0	20.0	41.0
39	34.17570682014722	39.0	34.0	40.0	14.0	41.0
40	34.13338864039788	38.0	33.0	40.0	15.0	41.0
41	34.0830723143317	38.0	33.0	40.0	18.0	41.0
42	33.982375528072126	38.0	34.0	40.0	13.0	41.0
43	34.0241454644759	38.0	34.0	40.0	13.0	41.0
44	34.34443336464182	39.0	34.0	41.0	15.0	41.0
45	34.32339530207174	39.0	34.0	40.0	17.0	41.0
46	34.26163827524712	38.0	34.0	40.0	16.0	41.0
47	34.00622307918289	38.0	33.0	40.0	16.0	41.0
48	33.9851374332281	38.0	34.0	40.0	15.0	41.0
49	34.01709277932481	38.0	34.0	40.0	15.0	41.0
50	33.895386894294546	38.0	34.0	40.0	14.0	41.0
51	33.040472771959735	37.0	32.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	9.0
10	24.0
11	15.0
12	10.0
13	22.0
14	31.0
15	34.0
16	50.0
17	112.0
18	220.0
19	542.0
20	1398.0
21	3273.0
22	5551.0
23	7102.0
24	8295.0
25	9822.0
26	10680.0
27	10446.0
28	9457.0
29	8535.0
30	9200.0
31	10993.0
32	13931.0
33	17844.0
34	27611.0
35	33396.0
36	36398.0
37	51510.0
38	88264.0
39	118549.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.66810796049338	15.515079795267313	16.732180022426256	16.08463222181305
2	32.0637534601396	38.41489401318267	16.78845254695239	12.73289997972534
3	24.028988625502212	37.92623334064324	26.36884984752628	11.675928186328258
4	22.457288740116105	18.094719899371487	45.22531767081401	14.222673689698404
5	35.0557139369665	23.891410578407076	25.024308903058163	16.028566581568267
6	20.030743004208027	41.82124370554574	26.98329616312412	11.164717127122117
7	57.83760411451458	5.15245302692392	32.77977995787836	4.230162900683132
8	55.76338230973887	20.854556212528085	17.635023026220516	5.747038451512531
9	51.24585714226605	6.723118490903299	18.007414732643444	24.023609634187213
10	40.82261327948825	19.5658740240234	25.48586773474125	14.125644961747097
11	33.683450498798	19.74917349729602	29.520318105271	17.047057898634975
12	22.15772030072699	17.526822547076517	33.19727243763474	27.118184714561757
13	27.119839788812527	19.311406357967734	39.168159681563715	14.400594171656026
14	17.617644746587445	33.17120501818513	30.04311468423252	19.168035550994905
15	14.511691030738866	21.820912690695586	40.951295302485505	22.71610097608004
16	16.043876018387877	23.369855305133626	30.478192327903308	30.108076348575185
17	16.782866671356047	29.650862086800366	34.564984421613616	19.001286820229975
18	16.68190714205916	23.068631791493747	33.54938948448575	26.700071581961343
19	17.113674637228414	24.486409771558375	31.165461910534965	27.23445368067825
20	24.435930006909935	23.354338984032672	37.36805954957154	14.84167145948585
21	19.010803497171892	33.289749711396425	31.769977780628185	15.929469010803496
22	16.67776945643224	20.377481059744042	40.23878583752963	22.70596364629408
23	22.693757473694664	26.35643679064552	33.883093830296964	17.066711905362855
24	19.786412667938315	27.268796471381695	30.468054998117356	22.476735862562634
25	15.228958834165699	36.47804337122074	29.649413896830946	18.643583897782616
26	16.034979994289994	21.964076613387068	39.69343887190139	22.30750452042155
27	24.97279471700299	24.08712310856046	30.195381515303232	20.74470065913332
28	14.71650646927148	28.27777111150649	36.2641250243089	20.741597394913132
29	19.712554979497767	19.735312250445837	39.18243469697659	21.369698073079803
30	15.977466164075786	31.705843653410902	31.493373496468486	20.823316686044826
31	21.61857986353913	20.403341594912302	27.236108754929017	30.74196978661955
32	28.415969811445667	26.634696149055987	27.087772725203884	17.861561314294462
33	22.624865008006424	25.732680682387116	25.820606501959194	25.82184780764727
34	23.70314588238215	22.403912595528816	30.377439682887776	23.51550183920126
35	22.920088877487267	26.27409684666978	26.738138289729026	24.067675986113926
36	18.025827433683244	31.806182529863747	28.658851957745956	21.509138078707053
37	21.150607619134313	23.834724285318252	30.044562874201947	24.97010522134549
38	17.734534365547976	28.624922935605195	23.630529499629677	30.010013199217152
39	27.31286282330841	26.236650791746147	26.985365005937577	19.465121379007865
40	18.509729767751708	29.147926398848067	28.646645785146536	23.695698048253693
41	20.601743620723184	28.830565911263196	25.120096325321395	25.447594142692225
42	19.803377179008695	27.224523235173635	32.016790728274046	20.955308857543624
43	21.36307777607673	28.562443882638682	26.952470405203556	23.122007936081033
44	21.042614024271664	22.833197479321914	31.30883271750779	24.81535577889863
45	19.362506775460215	22.26571389558964	29.10427381548405	29.267505513466098
46	27.416304963981446	27.374514339149542	26.289199399208048	18.919981297660968
47	16.700526727380307	27.06398103284909	36.60031198149627	19.63518025827434
48	21.41024739222363	26.27802764801536	27.217075401045182	25.094649558715826
49	20.067775290568974	21.66595636396738	36.53204016865207	21.73422817681158
50	21.181019608492186	23.13628295149391	31.636123650597277	24.046573789416627
51	19.67924661020105	21.074681087880304	30.297375466006844	28.948696835911804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	97.0
1	381.5
2	666.0
3	7286.5
4	13907.0
5	9174.0
6	4441.0
7	4151.0
8	3861.0
9	3792.5
10	3724.0
11	3570.0
12	3416.0
13	3291.5
14	3167.0
15	2881.0
16	2595.0
17	2461.5
18	2328.0
19	2293.5
20	2259.0
21	2233.5
22	2208.0
23	2352.0
24	2496.0
25	2541.5
26	3347.0
27	4107.0
28	5055.5
29	6004.0
30	6730.5
31	7457.0
32	7892.0
33	8327.0
34	9537.5
35	10748.0
36	11205.5
37	11663.0
38	12788.5
39	13914.0
40	16058.0
41	18202.0
42	19487.0
43	20772.0
44	24155.5
45	27539.0
46	57393.0
47	87247.0
48	65945.0
49	44643.0
50	44118.5
51	43594.0
52	38062.5
53	32531.0
54	29828.0
55	27125.0
56	23888.0
57	20651.0
58	17692.0
59	14733.0
60	13264.0
61	11795.0
62	10344.0
63	8893.0
64	7670.5
65	6448.0
66	4932.0
67	3416.0
68	2974.0
69	2532.0
70	1994.5
71	1457.0
72	1117.5
73	778.0
74	655.5
75	409.0
76	285.0
77	212.5
78	140.0
79	79.5
80	19.0
81	20.0
82	21.0
83	27.0
84	33.0
85	17.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	483362.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.98967615708959
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.17294816288293	24.687376198184868
2	8.974559826294582	5.741865249911798
3	3.164248844023518	3.036698874022723
4	1.5796313800680664	2.021275851838158
5	0.9858158696983134	1.5767965211084332
6	0.7304625006500188	1.4020355304415169
7	0.5945438115459594	1.331348479478986
8	0.5224935330648616	1.3371519133534804
9	0.443846304990051	1.2778649604137295
>10	5.65855894453793	36.33020648949535
>50	0.1069965843319819	2.334381849691564
>100	0.05349829216599095	3.070280857470952
>500	0.0052193455771698495	1.0842301036341238
>1k	0.004566927380023619	4.2757694192785936
>5k	6.524181971462312E-4	1.7940408028563866
>10k+	0.0019572545914386934	8.698676898819373
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	14430	2.9853401798238175	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	14069	2.9106549542578852	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	13180	2.7267348281412276	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8596	1.778377282450834	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	4483	0.9274622332744403	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGAGAAT	3600	0.7447834128458588	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	3167	0.6552025190230096	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTA	2749	0.5687248894203517	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	2608	0.5395542057505555	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTC	2396	0.49569473810518827	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	1484	0.30701627351757066	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATG	791	0.16364546654474285	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	770	0.15930089663647534	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCCGAGAA	681	0.14088819559667495	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	654	0.13530232000033102	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGT	627	0.12971644440398708	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTAT	583	0.1206135360247599	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCC	553	0.11440700758437775	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	536	0.11088997480149454	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0688428134607188E-4	0.0	0.0	0.16592119363954966	0.0
2	2.0688428134607188E-4	0.0	0.0	1.3975033204927156	0.0
3	2.0688428134607188E-4	0.0	0.0	1.6304550212883926	0.0
4	2.0688428134607188E-4	0.0	0.0	2.4716465092415207	0.0
5	2.0688428134607188E-4	0.0	0.0	6.123567843562382	0.0
6	2.0688428134607188E-4	0.0	0.0	6.903728468518419	0.0
7	2.0688428134607188E-4	0.0	0.0	8.082555103628337	0.0
8	2.0688428134607188E-4	0.0	0.0	9.269863994273443	0.0
9	2.0688428134607188E-4	0.0	0.0	9.56074329384602	0.0
10	2.0688428134607188E-4	0.0	0.0	13.264178814222053	0.0
11	2.0688428134607188E-4	0.0	0.0	14.236121167985898	0.0
12	2.0688428134607188E-4	0.0	0.0	18.235814979249508	0.0
13	2.0688428134607188E-4	0.0	0.0	18.714545206284317	0.0
14	2.0688428134607188E-4	0.0	0.0	18.988046226223823	0.0
15	2.0688428134607188E-4	0.0	0.0	19.852822522250406	0.0
16	2.0688428134607188E-4	0.0	0.0	20.499749670019572	0.0
17	2.0688428134607188E-4	0.0	0.0	21.144607974975276	0.0
18	2.0688428134607188E-4	0.0	0.0	21.76526081901349	0.0
19	2.0688428134607188E-4	0.0	0.0	23.01670383687588	0.0
20	2.0688428134607188E-4	0.0	0.0	23.59660047748892	0.0
21	2.0688428134607188E-4	0.0	0.0	24.0716067874595	0.0
22	2.0688428134607188E-4	0.0	0.0	24.647158858164275	0.0
23	2.0688428134607188E-4	0.0	0.0	25.10561442562717	0.0
24	2.0688428134607188E-4	0.0	0.0	25.50634927859451	0.0
25	2.0688428134607188E-4	0.0	0.0	25.87874098501744	0.0
26	2.0688428134607188E-4	0.0	0.0	26.278855185140742	0.0
27	2.0688428134607188E-4	0.0	0.0	26.749723809484404	0.0
28	2.0688428134607188E-4	0.0	0.0	27.07577343688581	0.0
29	2.0688428134607188E-4	0.0	0.0	27.42085641817106	0.0
30	2.0688428134607188E-4	0.0	0.0	27.784558984777455	0.0
31	2.0688428134607188E-4	0.0	0.0	28.159433302576538	0.0
32	2.0688428134607188E-4	0.0	0.0	28.51961883640005	0.0
33	2.0688428134607188E-4	0.0	0.0	28.876907990284714	0.0
34	2.0688428134607188E-4	0.0	0.0	29.227576847166308	0.0
35	2.0688428134607188E-4	0.0	0.0	29.576383745515784	0.0
36	2.0688428134607188E-4	0.0	0.0	29.92208737964507	0.0
37	2.0688428134607188E-4	0.0	0.0	30.254136651205513	0.0
38	2.0688428134607188E-4	0.0	0.0	30.585565269921922	0.0
39	2.0688428134607188E-4	0.0	0.0	30.946785225152162	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	35	1.2099736E-7	45.000004	16
CGGCGAT	230	0.0	45.000004	31
CGAGCCC	35	1.2099736E-7	45.000004	34
ACCCGTC	35	1.2099736E-7	45.000004	39
CGATCAC	70	0.0	45.000004	34
TTGTGCG	25	3.88744E-5	45.0	1
GCGAACC	55	1.8189894E-12	45.0	33
GTTTACG	20	7.029225E-4	45.0	1
TAGCGTG	25	3.88744E-5	45.0	1
GTTAGCG	20	7.029225E-4	45.0	1
ACGGGTC	25	3.88744E-5	45.0	5
ATCGTAA	25	3.88744E-5	45.0	41
CTACGTG	20	7.029225E-4	45.0	14
CGGGCAA	20	7.029225E-4	45.0	6
ACTCGTA	20	7.029225E-4	45.0	40
TACTAGG	25	3.88744E-5	45.0	2
TAGTGCG	40	6.8012014E-9	45.0	1
GTGACGC	20	7.029225E-4	45.0	9
CAATCGA	170	0.0	45.0	41
CGATCGA	130	0.0	44.999996	41
>>END_MODULE