SRR3551295_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551295_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335464
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGC	10394	3.0983950587842513	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCG	9333	2.7821167099897455	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTC	8339	2.485810698018267	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8183	2.439307943624353	No Hit
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	3620	1.0791023776023656	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGATAGTGT	2433	0.725264111797391	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT	2286	0.6814442086185104	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTA	1874	0.5586292418858656	No Hit
GAACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCT	1737	0.5177902845014667	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTC	1521	0.4534018553406625	No Hit
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	991	0.2954117282331338	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	926	0.27603558056900296	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATG	577	0.17200057234159255	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTT	514	0.15322061383635802	No Hit
TCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	503	0.14994157346242815	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGATAGTG	503	0.14994157346242815	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCC	472	0.14070064149953496	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTAT	425	0.12669019626547112	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	425	0.12669019626547112	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGT	416	0.12400734505043759	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	401	0.1195359263587151	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	398	0.11864164262037058	No Hit
ACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	350	0.10433310280685856	TruSeq Adapter, Index 14 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGG	20	7.0261356E-4	45.000004	3
AGATCGA	20	7.0261356E-4	45.000004	24
GCGACCG	40	6.7902874E-9	45.000004	33
GTCGAAT	145	0.0	45.000004	43
GGTCGAA	35	1.208482E-7	45.000004	42
TATGGGT	20	7.0261356E-4	45.000004	4
CAGTTAG	20	7.0261356E-4	45.000004	44
TAAACGG	20	7.0261356E-4	45.000004	2
GCCGGAT	20	7.0261356E-4	45.000004	33
GGATATC	20	7.0261356E-4	45.000004	8
CCTGTCG	20	7.0261356E-4	45.000004	40
CCGTCGA	80	0.0	45.000004	41
CGCTAGG	20	7.0261356E-4	45.000004	2
TACGAAA	20	7.0261356E-4	45.000004	20
TCATGCC	20	7.0261356E-4	45.000004	38
GCCTCTC	20	7.0261356E-4	45.000004	45
GAGCCGA	20	7.0261356E-4	45.000004	9
CGGTCGA	35	1.208482E-7	45.000004	41
ATACGGT	20	7.0261356E-4	45.000004	4
CACCGCG	40	6.7902874E-9	45.000004	38

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content