##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551295_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	335464
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.488836357999666	31.0	31.0	34.0	30.0	34.0
2	31.647589607230582	31.0	31.0	34.0	30.0	34.0
3	31.64406314835571	31.0	31.0	34.0	30.0	34.0
4	35.41272088808337	37.0	35.0	37.0	33.0	37.0
5	34.289813512031095	37.0	35.0	37.0	32.0	37.0
6	34.712887224858704	37.0	35.0	37.0	32.0	37.0
7	35.34432904872058	37.0	35.0	37.0	33.0	37.0
8	34.739009252855745	36.0	35.0	37.0	32.0	37.0
9	36.49803257577564	39.0	35.0	39.0	32.0	39.0
10	36.47126666348699	38.0	35.0	39.0	32.0	39.0
11	36.72327880189827	39.0	35.0	39.0	33.0	39.0
12	36.66157620489829	39.0	35.0	39.0	32.0	39.0
13	36.59539026542341	39.0	35.0	39.0	32.0	39.0
14	37.63992559559297	39.0	36.0	41.0	32.0	41.0
15	37.62787363174588	40.0	36.0	41.0	32.0	41.0
16	37.89223582858369	40.0	36.0	41.0	33.0	41.0
17	37.599340018601104	39.0	36.0	41.0	32.0	41.0
18	37.50436410464312	39.0	36.0	40.0	33.0	41.0
19	37.3567953640331	39.0	36.0	40.0	32.0	41.0
20	37.00111189278134	39.0	35.0	40.0	32.0	41.0
21	37.159811484987955	39.0	35.0	40.0	32.0	41.0
22	37.20404275868648	39.0	35.0	41.0	32.0	41.0
23	37.129873846373975	39.0	35.0	41.0	32.0	41.0
24	37.037622516872155	39.0	35.0	41.0	32.0	41.0
25	36.988523358691246	39.0	35.0	40.0	32.0	41.0
26	37.00693666086376	39.0	35.0	41.0	32.0	41.0
27	36.83416700450719	39.0	35.0	41.0	32.0	41.0
28	36.659665418644025	39.0	35.0	41.0	31.0	41.0
29	36.68648498795698	39.0	35.0	41.0	31.0	41.0
30	36.38077111105812	39.0	35.0	40.0	30.0	41.0
31	36.173207855388355	38.0	35.0	40.0	30.0	41.0
32	35.782948393866405	39.0	35.0	40.0	27.0	41.0
33	35.49808325185415	39.0	35.0	40.0	25.0	41.0
34	35.17720828464455	38.0	35.0	40.0	23.0	41.0
35	35.043861636420004	39.0	35.0	41.0	23.0	41.0
36	34.82062456776286	38.0	35.0	40.0	21.0	41.0
37	34.69873071328071	38.0	35.0	40.0	20.0	41.0
38	34.43340567095128	38.0	34.0	40.0	20.0	41.0
39	34.43295554813631	38.0	34.0	40.0	18.0	41.0
40	34.33580950563995	38.0	34.0	40.0	18.0	41.0
41	34.20260296186774	38.0	34.0	40.0	19.0	41.0
42	34.13661972670689	38.0	34.0	40.0	18.0	41.0
43	34.14003589058736	38.0	34.0	40.0	18.0	41.0
44	34.35542412896764	38.0	34.0	40.0	18.0	41.0
45	34.32780864712756	38.0	34.0	40.0	20.0	41.0
46	34.28237009038228	38.0	34.0	40.0	20.0	41.0
47	34.05540683948203	37.0	33.0	40.0	20.0	41.0
48	34.05659623685403	37.0	33.0	40.0	20.0	41.0
49	34.089556554503616	37.0	34.0	40.0	20.0	41.0
50	33.884294588987196	37.0	33.0	40.0	18.0	41.0
51	32.98182219254525	36.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	8.0
10	15.0
11	14.0
12	7.0
13	18.0
14	19.0
15	27.0
16	47.0
17	74.0
18	173.0
19	492.0
20	1192.0
21	1965.0
22	2554.0
23	3280.0
24	4111.0
25	5089.0
26	6398.0
27	7256.0
28	6622.0
29	6703.0
30	7221.0
31	8369.0
32	10464.0
33	13927.0
34	22225.0
35	27875.0
36	27694.0
37	37694.0
38	62491.0
39	71402.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.415031121074094	15.848794505520711	17.846922471561776	17.889251901843416
2	33.07091073855913	35.97465003696373	17.615601077909997	13.338838146567142
3	24.07769537118737	34.96589798011113	27.773770061765195	13.182636586936303
4	23.21262490162879	17.4039539265018	44.25243841365988	15.130982758209525
5	31.689838551975768	23.841306369684972	27.155819998569147	17.31303507977011
6	19.421159945627547	39.35951398659767	29.01801683638185	12.201309231392935
7	57.98476140509861	5.305189230439034	31.72233086113562	4.987718503326735
8	54.82972837621921	18.28422721961224	19.915996947511506	6.970047456657048
9	50.411966708797365	7.380821787136623	20.947404192402168	21.259807311663845
10	39.41883480790786	18.74746619607469	27.766317697278993	14.067381298738463
11	32.46279779648487	18.682481577754988	31.186952996446713	17.667767629313428
12	24.773448119619392	16.03122838814299	34.76855936851644	24.426764123721174
13	24.516490592135074	20.336012209953974	39.421517659122884	15.725979538788067
14	17.33449788949038	30.82745093363222	31.61889204206711	20.219159134810294
15	14.510349843798439	21.482483962511626	44.14631674337634	19.860849450313594
16	15.267808170176234	22.710931724417524	33.278682660434505	28.74257744497174
17	15.305069992607253	26.072246202275057	37.27732334915222	21.34536045596547
18	15.970118999356114	22.49034173562588	36.06944411322824	25.470095151789764
19	17.355960699210645	23.81268929005795	33.02798511911859	25.803364891612812
20	22.149321536737176	22.954176901247227	39.30973219182982	15.58676937018577
21	18.775487086542817	31.468950468604678	33.400901438008255	16.35466100684425
22	15.655927312617749	21.42942312736985	38.61636420003339	24.298285359979012
23	19.880523692557176	26.45261488565092	35.02402642310352	18.642834998688386
24	19.97233682302721	23.006939641809552	32.966875730331715	24.053847804831516
25	15.316099492046837	33.81286814680562	30.438437507452363	20.43259485369518
26	17.751234111558915	21.341485226432646	37.11068847924069	23.796592182767746
27	23.86813488183531	25.503481744687956	30.781842462976655	19.84654091050008
28	14.738392197076289	27.21275606324375	37.645768249350155	20.403083490329813
29	23.17506498461832	20.20008108172561	35.2151050485298	21.409748885126273
30	20.389967328834093	30.27448548875587	32.68398397443541	16.651563207974625
31	23.69732668781151	24.018076455297738	27.89837359597453	24.386223260916225
32	25.756862137218896	26.645780173133332	31.473719981875846	16.12363770777192
33	23.61862971883719	25.75775642095724	26.959077576133357	23.66453628407221
34	26.93910523931033	22.245606085898935	30.06015548613264	20.7551331886581
35	25.16097107290201	21.73705673336036	31.40664870150001	21.695323492237616
36	27.65810936493931	28.863305749648248	25.965528342832616	17.51305654257983
37	24.074714425392887	22.913039849283383	35.76926287172394	17.242982853599788
38	18.978191400567574	31.413206782247872	26.636837335749885	22.97176448143467
39	25.965528342832616	23.927753797724943	32.36263801779028	17.74407984165216
40	21.269048243626738	24.897157370090383	33.47840602866478	20.355388357618104
41	25.698435599647055	23.832363532301528	27.018696492022986	23.450504376028427
42	21.360861374096775	21.744211003267118	36.67040278539575	20.22452483724036
43	28.4155676913171	22.38660482197792	27.315300598573916	21.882526888131064
44	22.93778169937758	22.26408794982472	32.13042234040016	22.66770801039754
45	20.11870126153626	21.97195526196552	30.487325018481865	27.42201845801636
46	27.67510075596785	25.709763193666085	28.57176925094794	18.04336679941812
47	17.99477738296807	23.746810387999908	39.60156678510958	18.65684544392245
48	22.502265518803807	23.86485584146138	30.00620036725252	23.626678272482295
49	20.399804449955884	19.492702644695107	39.33924355519519	20.768249350153816
50	22.638792836191065	20.475818567715166	33.01486895762287	23.870519638470896
51	21.037130660816064	19.29387356020318	30.17671046669687	29.492285312283883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	142.0
1	317.0
2	492.0
3	6614.0
4	12736.0
5	8126.0
6	3516.0
7	3305.5
8	3095.0
9	2911.0
10	2727.0
11	2659.0
12	2591.0
13	2466.5
14	2342.0
15	2141.5
16	1941.0
17	1744.0
18	1547.0
19	1559.0
20	1571.0
21	1517.5
22	1464.0
23	1481.0
24	1498.0
25	1607.0
26	2230.0
27	2744.0
28	3142.0
29	3540.0
30	4041.5
31	4543.0
32	4833.0
33	5123.0
34	6009.5
35	6896.0
36	6898.0
37	6900.0
38	7362.5
39	7825.0
40	9819.5
41	11814.0
42	13311.5
43	14809.0
44	17552.0
45	20295.0
46	40681.0
47	61067.0
48	47005.0
49	32943.0
50	32077.5
51	31212.0
52	26036.0
53	20860.0
54	18328.0
55	15796.0
56	13905.5
57	12015.0
58	11044.5
59	10074.0
60	9468.0
61	8862.0
62	7920.5
63	6979.0
64	5916.5
65	4854.0
66	4037.0
67	3220.0
68	2703.5
69	2187.0
70	1756.0
71	1325.0
72	1145.5
73	966.0
74	724.5
75	433.5
76	384.0
77	314.0
78	244.0
79	165.5
80	87.0
81	55.5
82	24.0
83	18.0
84	12.0
85	6.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	335464.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.06169999010685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.69698874007501	22.891537544184928
2	11.308353919855188	7.025133936809813
3	4.022582495259372	3.7484475195960605
4	2.074523964855588	2.5775296407452495
5	1.1769710073548856	1.827936016375565
6	0.7809419516996475	1.455443076802978
7	0.5714886862760187	1.242598708459219
8	0.5056620765200195	1.256537897379144
9	0.42188365907044645	1.1793981283897257
>10	5.248112852863261	34.50149886005908
>50	0.12467010762341495	2.584638360460332
>100	0.051862764771340615	3.0626554994019948
>500	0.005984165165923917	1.2435261151729322
>1k	0.005984165165923917	4.173278599276175
>5k	0.0029920825829619583	8.009807600347822
>10k+	9.973608609873195E-4	3.2200324965389777
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGC	10394	3.0983950587842513	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCG	9333	2.7821167099897455	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTC	8339	2.485810698018267	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8183	2.439307943624353	No Hit
GCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	3620	1.0791023776023656	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGATAGTGT	2433	0.725264111797391	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT	2286	0.6814442086185104	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTA	1874	0.5586292418858656	No Hit
GAACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCT	1737	0.5177902845014667	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTC	1521	0.4534018553406625	No Hit
CCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	991	0.2954117282331338	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	926	0.27603558056900296	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATG	577	0.17200057234159255	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTT	514	0.15322061383635802	No Hit
TCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	503	0.14994157346242815	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGATAGTG	503	0.14994157346242815	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCC	472	0.14070064149953496	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTAT	425	0.12669019626547112	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	425	0.12669019626547112	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGT	416	0.12400734505043759	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	401	0.1195359263587151	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	398	0.11864164262037058	No Hit
ACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGC	350	0.10433310280685856	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29332506617699666	0.0
2	0.0	0.0	0.0	1.998724155199962	0.0
3	0.0	0.0	0.0	2.3779004602580307	0.0
4	0.0	0.0	0.0	3.3625068561753273	0.0
5	0.0	0.0	0.0	7.139663272363055	0.0
6	0.0	0.0	0.0	7.960019555004412	0.0
7	0.0	0.0	0.0	9.391469725514511	0.0
8	0.0	0.0	0.0	10.903703527055065	0.0
9	0.0	0.0	0.0	11.342796842582214	0.0
10	0.0	0.0	0.0	15.249326306250447	0.0
11	0.0	0.0	0.0	16.732644933584528	0.0
12	0.0	0.0	0.0	21.32717668661913	0.0
13	0.0	0.0	0.0	22.03843035318246	0.0
14	0.0	0.0	0.0	22.422674266091146	0.0
15	0.0	0.0	0.0	23.449311997710634	0.0
16	0.0	0.0	0.0	24.382944220542292	0.0
17	0.0	0.0	0.0	25.37887821047862	0.0
18	0.0	0.0	0.0	26.358715093124747	0.0
19	0.0	0.0	0.0	27.82355185653304	0.0
20	0.0	0.0	0.0	28.559547373190565	0.0
21	0.0	0.0	0.0	29.204027853957502	0.0
22	0.0	0.0	0.0	29.955524288746332	0.0
23	0.0	0.0	0.0	30.549924880165978	0.0
24	0.0	0.0	0.0	31.115708391958602	0.0
25	0.0	0.0	0.0	31.546455059261202	0.0
26	0.0	0.0	0.0	31.963191281329742	0.0
27	0.0	0.0	0.0	32.48127966041066	0.0
28	0.0	0.0	0.0	32.87685116733837	0.0
29	0.0	0.0	0.0	33.315646388286076	0.0
30	0.0	0.0	0.0	33.789020580449765	0.0
31	0.0	0.0	0.0	34.23169103093029	0.0
32	0.0	0.0	0.0	34.681515751317576	0.0
33	0.0	0.0	0.0	35.106300527031216	0.0
34	0.0	0.0	0.0	35.532575775642094	0.0
35	0.0	0.0	0.0	35.99104523883338	0.0
36	0.0	0.0	0.0	36.40241575847185	0.0
37	0.0	0.0	0.0	36.8248157775499	0.0
38	0.0	0.0	0.0	37.23767677008561	0.0
39	0.0	0.0	0.0	37.672000572341595	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGG	20	7.0261356E-4	45.000004	3
AGATCGA	20	7.0261356E-4	45.000004	24
GCGACCG	40	6.7902874E-9	45.000004	33
GTCGAAT	145	0.0	45.000004	43
GGTCGAA	35	1.208482E-7	45.000004	42
TATGGGT	20	7.0261356E-4	45.000004	4
CAGTTAG	20	7.0261356E-4	45.000004	44
TAAACGG	20	7.0261356E-4	45.000004	2
GCCGGAT	20	7.0261356E-4	45.000004	33
GGATATC	20	7.0261356E-4	45.000004	8
CCTGTCG	20	7.0261356E-4	45.000004	40
CCGTCGA	80	0.0	45.000004	41
CGCTAGG	20	7.0261356E-4	45.000004	2
TACGAAA	20	7.0261356E-4	45.000004	20
TCATGCC	20	7.0261356E-4	45.000004	38
GCCTCTC	20	7.0261356E-4	45.000004	45
GAGCCGA	20	7.0261356E-4	45.000004	9
CGGTCGA	35	1.208482E-7	45.000004	41
ATACGGT	20	7.0261356E-4	45.000004	4
CACCGCG	40	6.7902874E-9	45.000004	38
>>END_MODULE