SRR3551294_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551294_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742766
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	19759	2.6601917696825113	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	18784	2.5289256643411253	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	18226	2.453801062514978	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15898	2.140377992530622	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	6409	0.8628558657773782	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	5223	0.7031824289210868	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACAGAT	4687	0.631019728959053	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCT	3961	0.5332769674433132	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTA	3700	0.498138040782696	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC	3475	0.4678458626269915	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTT	1351	0.18188770083714118	No Hit
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	1312	0.17663705662348572	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATG	1200	0.1615582834970906	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC	929	0.12507303780733098	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTCACAGATCGT	878	0.11820681075870464	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	862	0.11605270031207675	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTCACAGA	780	0.1050128842731089	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTAT	762	0.10258951002065253	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	20	7.031672E-4	45.000004	1
GATAACG	20	7.031672E-4	45.000004	1
CTATCGC	20	7.031672E-4	45.000004	25
ACACCGT	35	1.2111559E-7	45.000004	39
CAACCGG	20	7.031672E-4	45.000004	45
TGTTGCG	35	1.2111559E-7	45.000004	1
TCGTGTA	20	7.031672E-4	45.000004	17
CATCGTA	55	1.8189894E-12	45.000004	40
CATCGCT	20	7.031672E-4	45.000004	13
ACATCGC	20	7.031672E-4	45.000004	24
ATCGCTA	20	7.031672E-4	45.000004	14
ATTTACG	25	3.889468E-5	45.0	1
CGGGTTC	45	3.8380676E-10	45.0	6
ATTAGCG	25	3.889468E-5	45.0	1
TCGTGTC	25	3.889468E-5	45.0	32
TATAGCG	25	3.889468E-5	45.0	1
ACGTATT	25	3.889468E-5	45.0	35
CGATCGA	385	0.0	45.0	41
TCGAATA	25	3.889468E-5	45.0	44
CGTTTTT	6765	0.0	43.43681	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content