##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551294_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742766
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.459925467778547	31.0	31.0	34.0	30.0	34.0
2	31.63011365625244	31.0	31.0	34.0	30.0	34.0
3	31.60898317909005	31.0	31.0	34.0	30.0	34.0
4	35.41090599192747	37.0	35.0	37.0	33.0	37.0
5	34.19997145803658	37.0	35.0	37.0	32.0	37.0
6	34.634591513343366	37.0	35.0	37.0	32.0	37.0
7	35.31595145712109	37.0	35.0	37.0	32.0	37.0
8	34.80373899720774	37.0	35.0	37.0	32.0	37.0
9	36.53305617112253	39.0	35.0	39.0	32.0	39.0
10	36.32915884679697	38.0	35.0	39.0	32.0	39.0
11	36.60248449713638	39.0	35.0	39.0	32.0	39.0
12	36.634170115487244	39.0	35.0	39.0	32.0	39.0
13	36.63055255625594	39.0	35.0	39.0	32.0	39.0
14	37.72042473672732	40.0	37.0	41.0	32.0	41.0
15	37.69877861937676	40.0	36.0	41.0	32.0	41.0
16	37.92925901293274	40.0	37.0	41.0	33.0	41.0
17	37.62115928838961	40.0	36.0	41.0	32.0	41.0
18	37.51905041426237	39.0	36.0	40.0	32.0	41.0
19	37.38447371042832	39.0	36.0	40.0	32.0	41.0
20	36.96044245428574	39.0	35.0	41.0	31.0	41.0
21	37.17232614309217	39.0	35.0	40.0	32.0	41.0
22	37.21198600905265	39.0	35.0	41.0	32.0	41.0
23	37.036842020232484	39.0	35.0	41.0	31.0	41.0
24	37.003402148186645	39.0	35.0	41.0	31.0	41.0
25	37.00564511568919	39.0	35.0	40.0	32.0	41.0
26	36.931278491476455	39.0	35.0	41.0	31.0	41.0
27	36.78763702161919	39.0	35.0	41.0	31.0	41.0
28	36.64512376710835	39.0	35.0	41.0	30.0	41.0
29	36.70413966175081	39.0	35.0	41.0	31.0	41.0
30	36.43208628289394	39.0	35.0	41.0	30.0	41.0
31	36.34122051897906	39.0	35.0	40.0	30.0	41.0
32	35.83924950792039	39.0	35.0	41.0	26.0	41.0
33	35.5252932956005	39.0	35.0	41.0	24.0	41.0
34	35.27672914484508	39.0	35.0	41.0	23.0	41.0
35	35.06530185818952	39.0	35.0	41.0	20.0	41.0
36	34.95209662262408	39.0	35.0	41.0	20.0	41.0
37	34.72803951715614	39.0	35.0	41.0	18.0	41.0
38	34.923415988346264	39.0	35.0	41.0	20.0	41.0
39	34.76963000460441	39.0	35.0	40.0	20.0	41.0
40	34.79775191648514	39.0	34.0	40.0	21.0	41.0
41	34.404463855373024	38.0	34.0	40.0	18.0	41.0
42	34.46271638712596	39.0	35.0	40.0	18.0	41.0
43	34.485308966754	39.0	35.0	40.0	18.0	41.0
44	34.724903940137274	39.0	35.0	41.0	18.0	41.0
45	34.68076217812878	39.0	35.0	41.0	18.0	41.0
46	34.61668412393674	39.0	35.0	40.0	19.0	41.0
47	34.29130708729263	38.0	34.0	40.0	18.0	41.0
48	34.41140009101117	38.0	34.0	40.0	18.0	41.0
49	34.4809711268421	38.0	34.0	40.0	18.0	41.0
50	34.30764870766836	38.0	34.0	40.0	17.0	41.0
51	33.539945285594655	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	18.0
10	21.0
11	29.0
12	28.0
13	23.0
14	38.0
15	59.0
16	83.0
17	167.0
18	333.0
19	785.0
20	1744.0
21	3567.0
22	6034.0
23	9169.0
24	11812.0
25	13803.0
26	15798.0
27	16274.0
28	14030.0
29	13047.0
30	13524.0
31	16269.0
32	20649.0
33	27531.0
34	43431.0
35	54381.0
36	58631.0
37	81522.0
38	137766.0
39	182144.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.58824986604125	16.422803413188003	18.30940026872528	16.679546452045464
2	33.13048793294254	35.9934353484139	17.888271676409527	12.987805042234028
3	23.920184822676323	35.08722800989813	28.349843692360714	12.642743475064824
4	22.299216711588844	18.971385335354608	43.989493326296575	14.739904626759975
5	31.657480283157817	24.474868262682996	27.014295215451433	16.853356238707747
6	19.62111889881874	39.320997460842314	29.133401367321603	11.924482273017343
7	60.17197879278265	5.19113691256735	30.385343432521143	4.251540862128853
8	58.42418742914997	17.32241378846097	18.30886174111362	5.944537041275448
9	54.03828392791269	6.724594286760569	18.63534410568066	20.60177767964608
10	39.315073657114084	19.22758984660041	27.10799901987975	14.34933747640576
11	31.263547335230747	20.13204697037829	30.57409736040691	18.030308333984056
12	21.766747535563017	16.577226205830637	35.20085733595776	26.455168922648586
13	25.22665280855613	19.818758532296847	39.48605617381517	15.468532485331854
14	18.15336189324767	30.139774841605565	32.01385093017181	19.693012334974945
15	15.01549613202543	21.806733210728545	41.573793092306325	21.603977564939697
16	16.577764733442297	23.24567898907597	31.810018229159652	28.36653804832208
17	16.79182945907594	28.36424930597254	34.715913221660664	20.12800801329086
18	16.671333905967696	22.917850305479785	34.77932484793327	25.63149094061925
19	16.91784492020367	24.927500720280683	31.891739794228602	26.262914565287048
20	22.677801622583694	24.40351335413845	37.37839373369271	15.540291289585145
21	18.886028708906977	31.985847494365654	32.88343839109491	16.24468540563246
22	16.714820010608992	20.598142618267396	38.84884337732206	23.83819399380155
23	20.924355719028604	26.393911406822607	34.064429443458636	18.61730343069015
24	19.62084963501291	26.175538460295705	31.72856592789654	22.475045976794846
25	16.188409270214308	34.069680087672296	29.587649407754256	20.15426123435914
26	16.412032860954863	21.830159161835624	37.798310638882235	23.959497338327278
27	23.55896742715741	23.850176233160912	31.25102656825972	21.339829771421957
28	14.656971374564803	26.979829448305388	35.39418874854261	22.9690104285872
29	18.41414388919256	20.93781890932003	35.20179975927816	25.446237442209256
30	16.10412969898999	28.35092074758403	35.312601815376574	20.2323477380494
31	18.59118484152479	23.294146474125093	27.666452153168024	30.448216531182094
32	24.003118074871495	28.36748047164248	27.688935680954703	19.940465772531322
33	20.213768535447233	23.839944208539432	27.25057420506593	28.695713050947404
34	19.266767730348455	25.92916207796264	27.11338429599632	27.690685895692585
35	23.027575306354894	24.166561205009383	29.42191753526683	23.383945953368894
36	17.16906805104165	30.136005148323967	26.214312448335008	26.48061435229938
37	17.191013051216668	27.355721721241956	34.09579867683766	21.357466550703723
38	17.896618854390212	25.172261519778772	24.83285449253197	32.098265133299044
39	19.20981843541573	32.70155069025776	26.821636962381152	21.26699391194535
40	20.43012200343042	22.53078358460134	30.153372663799903	26.885721748168333
41	17.16879878723582	31.40423767377613	26.02057714004141	25.40638639894664
42	22.405441282988182	23.74462482127615	31.856062339956324	21.993871555779343
43	20.41652418123608	27.109479970811805	26.816386318167496	25.657609529784615
44	19.30150276130033	22.9283515939071	32.095707127143676	25.674438517648895
45	18.95684508983987	21.527102748375665	29.052218329864317	30.463833831920144
46	25.15018188770084	27.051857516364507	26.916552453935694	20.88140814199896
47	16.00450209083345	25.08690489333114	36.80284234873433	22.10575066710108
48	19.065627667394576	26.369004504783472	28.758316885802525	25.807050942019426
49	19.780388439966288	21.58459057092005	36.43139292859393	22.203628060519733
50	20.292932094360808	21.947827444982675	32.020717157220446	25.73852330343607
51	18.498692724222703	21.107185843186144	29.692931555833198	30.701189876757955
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	249.0
1	683.0
2	1117.0
3	12829.0
4	24541.0
5	15656.5
6	6772.0
7	6353.5
8	5935.0
9	5864.5
10	5794.0
11	5725.0
12	5656.0
13	5463.0
14	5270.0
15	4966.0
16	4662.0
17	4506.5
18	4351.0
19	4107.0
20	3863.0
21	3804.5
22	3746.0
23	3710.0
24	3674.0
25	4061.5
26	5312.5
27	6176.0
28	6901.5
29	7627.0
30	8457.5
31	9288.0
32	10504.0
33	11720.0
34	13290.0
35	14860.0
36	16344.0
37	17828.0
38	19676.0
39	21524.0
40	23183.0
41	24842.0
42	27641.0
43	30440.0
44	36478.5
45	42517.0
46	86635.5
47	130754.0
48	101635.0
49	72516.0
50	70857.0
51	69198.0
52	59372.0
53	49546.0
54	43855.5
55	38165.0
56	34142.5
57	30120.0
58	27057.0
59	23994.0
60	21799.0
61	19604.0
62	17106.0
63	14608.0
64	12103.5
65	9599.0
66	8021.5
67	6444.0
68	5109.5
69	3775.0
70	3544.0
71	3313.0
72	2591.0
73	1869.0
74	1528.5
75	911.5
76	635.0
77	472.0
78	309.0
79	212.5
80	116.0
81	95.5
82	75.0
83	52.5
84	30.0
85	18.0
86	6.0
87	3.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	742766.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.06666006829472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.68233784645705	25.356287988109262
2	9.486939994647	6.274028397826043
3	3.3857118589062543	3.3586254938294196
4	1.7450154129431321	2.30807325894902
5	1.0687889221226419	1.7670639986294256
6	0.8317455791024086	1.650182899649177
7	0.6539136843964829	1.513591906116126
8	0.5457737490213593	1.4437532026470472
9	0.44777173018980454	1.3325684031340603
>10	4.963049353239524	33.277751360509875
>50	0.12666004406598763	2.709026076060086
>100	0.05024873875940833	3.1734383912882387
>500	0.0066444613235581255	1.5565090508547028
>1k	0.00290695182905668	2.703258683292958
>5k	8.305576654447657E-4	1.597292746320415
>10k+	0.0016611153308895314	9.978548142784181
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	19759	2.6601917696825113	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	18784	2.5289256643411253	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	18226	2.453801062514978	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15898	2.140377992530622	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	6409	0.8628558657773782	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	5223	0.7031824289210868	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACAGAT	4687	0.631019728959053	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCT	3961	0.5332769674433132	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTA	3700	0.498138040782696	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC	3475	0.4678458626269915	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTT	1351	0.18188770083714118	No Hit
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	1312	0.17663705662348572	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATG	1200	0.1615582834970906	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC	929	0.12507303780733098	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTCACAGATCGT	878	0.11820681075870464	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	862	0.11605270031207675	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTCACAGA	780	0.1050128842731089	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTAT	762	0.10258951002065253	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3463190291424217E-4	0.0	0.0	0.18377254747794056	0.0
2	1.3463190291424217E-4	0.0	0.0	1.378765317744754	0.0
3	1.3463190291424217E-4	0.0	0.0	1.681148571690142	0.0
4	1.3463190291424217E-4	0.0	0.0	2.7777254209266444	0.0
5	1.3463190291424217E-4	0.0	0.0	6.46125428466031	0.0
6	1.3463190291424217E-4	0.0	0.0	7.297856929369411	0.0
7	1.3463190291424217E-4	0.0	0.0	8.489887797772111	0.0
8	1.3463190291424217E-4	0.0	0.0	9.902176459342511	0.0
9	1.3463190291424217E-4	0.0	0.0	10.292743609696728	0.0
10	1.3463190291424217E-4	0.0	0.0	13.708220354728137	0.0
11	1.3463190291424217E-4	0.0	0.0	14.964336008918018	0.0
12	1.3463190291424217E-4	0.0	0.0	18.730394229138113	0.0
13	1.3463190291424217E-4	0.0	0.0	19.336910951766775	0.0
14	1.3463190291424217E-4	0.0	0.0	19.624888592100337	0.0
15	1.3463190291424217E-4	0.0	0.0	20.478185592770807	0.0
16	1.3463190291424217E-4	0.0	0.0	21.189176672060917	0.0
17	1.3463190291424217E-4	0.0	0.0	21.95859799721581	0.0
18	1.3463190291424217E-4	0.0	0.0	22.76679331041001	0.0
19	1.3463190291424217E-4	0.0	0.0	24.007157031958922	0.0
20	1.3463190291424217E-4	0.0	0.0	24.614616177907983	0.0
21	1.3463190291424217E-4	0.0	0.0	25.21063161210933	0.0
22	1.3463190291424217E-4	0.0	0.0	25.9315854522151	0.0
23	1.3463190291424217E-4	0.0	0.0	26.541064076707872	0.0
24	1.3463190291424217E-4	0.0	0.0	27.03866358987891	0.0
25	1.3463190291424217E-4	0.0	0.0	27.47662117005894	0.0
26	1.3463190291424217E-4	0.0	0.0	27.905558412743716	0.0
27	1.3463190291424217E-4	0.0	0.0	28.41271679102167	0.0
28	1.3463190291424217E-4	0.0	0.0	28.836672653298617	0.0
29	1.3463190291424217E-4	0.0	0.0	29.26493673646882	0.0
30	1.3463190291424217E-4	0.0	0.0	29.746245789387235	0.0
31	2.6926380582848434E-4	0.0	0.0	30.18339557814978	0.0
32	2.6926380582848434E-4	0.0	0.0	30.601158372892673	0.0
33	2.6926380582848434E-4	0.0	0.0	31.032115094121163	0.0
34	2.6926380582848434E-4	0.0	0.0	31.453512950242743	0.0
35	2.6926380582848434E-4	0.0	0.0	31.901029395529683	0.0
36	2.6926380582848434E-4	0.0	0.0	32.32215798784543	0.0
37	2.6926380582848434E-4	0.0	0.0	32.723361058529875	0.0
38	2.6926380582848434E-4	0.0	0.0	33.14193164469025	0.0
39	2.6926380582848434E-4	0.0	0.0	33.55511695473406	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	20	7.031672E-4	45.000004	1
GATAACG	20	7.031672E-4	45.000004	1
CTATCGC	20	7.031672E-4	45.000004	25
ACACCGT	35	1.2111559E-7	45.000004	39
CAACCGG	20	7.031672E-4	45.000004	45
TGTTGCG	35	1.2111559E-7	45.000004	1
TCGTGTA	20	7.031672E-4	45.000004	17
CATCGTA	55	1.8189894E-12	45.000004	40
CATCGCT	20	7.031672E-4	45.000004	13
ACATCGC	20	7.031672E-4	45.000004	24
ATCGCTA	20	7.031672E-4	45.000004	14
ATTTACG	25	3.889468E-5	45.0	1
CGGGTTC	45	3.8380676E-10	45.0	6
ATTAGCG	25	3.889468E-5	45.0	1
TCGTGTC	25	3.889468E-5	45.0	32
TATAGCG	25	3.889468E-5	45.0	1
ACGTATT	25	3.889468E-5	45.0	35
CGATCGA	385	0.0	45.0	41
TCGAATA	25	3.889468E-5	45.0	44
CGTTTTT	6765	0.0	43.43681	1
>>END_MODULE