SRR3551290_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551290_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	338819
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5435	1.6041013048264707	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC	5383	1.5887538774389864	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCG	4800	1.416685604998539	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGC	4767	1.4069458914641741	No Hit
GCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	2784	0.8216776508991527	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT	1366	0.40316511175583425	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT	1112	0.32819883182466153	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCATGGTTGT	1108	0.32701826048716276	No Hit
GAACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCT	1029	0.30370197657156184	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTC	903	0.26651397944035016	No Hit
CCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	866	0.2555936945684864	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTA	853	0.2517568377216154	No Hit
CTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGCT	830	0.2449685525309974	TruSeq Adapter, Index 23 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	479	0.14137341766547923	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCC	429	0.12661627594674443	No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	378	0.11156399139363495	No Hit
ACTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC	364	0.10743199171238921	TruSeq Adapter, Index 23 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGCC	65	0.0	45.000004	31
TAATCAC	20	7.026237E-4	45.0	44
GTTGACG	25	3.8849634E-5	45.0	43
TACCCAC	20	7.026237E-4	45.0	40
ACCGGAT	20	7.026237E-4	45.0	4
GCGCCAA	20	7.026237E-4	45.0	29
TACGGTG	25	3.8849634E-5	45.0	29
CTAAGCA	20	7.026237E-4	45.0	44
CGACAAA	20	7.026237E-4	45.0	12
CCTCGAT	20	7.026237E-4	45.0	15
CTCGATC	20	7.026237E-4	45.0	16
ATGCGTG	25	3.8849634E-5	45.0	1
CGGCTAT	20	7.026237E-4	45.0	31
ATACGGT	25	3.8849634E-5	45.0	28
GCGTAAG	20	7.026237E-4	45.0	1
CGCCCGC	20	7.026237E-4	45.0	39
GCCCGAA	20	7.026237E-4	45.0	25
TACGTTG	25	3.8849634E-5	45.0	1
TTAAGCG	30	2.160954E-6	44.999996	1
CGTTTTT	2050	0.0	43.682926	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content