FastQCFastQC Report
Sat 18 Jun 2016
SRR3551288_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551288_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615488
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG221933.6057567328688784No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC219033.5586396485390455No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC206543.355711240511594No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86421.4040891130290112No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC63311.028614692731621RNA PCR Primer, Index 14 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT53700.872478423624831RNA PCR Primer, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCAACT49950.8115511594052198No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT48990.7959537797649996No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTA34770.5649175938442341No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTC34130.5545193407507538No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT17340.2817276697514818No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCACTCGAATGC15510.25199516481231155No Hit
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT11520.18716855568264532RNA PCR Primer, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC10840.1761204117708225RNA PCR Primer, Index 16 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATG10790.17530804824789434No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCACCAAC9570.15548637828844755No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCCCTCGAATGC8720.14167619839866902No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCACCAACTCGT8060.13095299989601747No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG7930.12884085473640428No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTAT7720.12542892794010607No Hit
GAATGATACGGCGACCACCGAGGAATCTGTCTCTTATACACATCTGACGCT7200.11698034730165331Illumina Single End PCR Primer 1 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACACCCACTCGAATGC7120.11568056566496829No Hit
GAATATGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC6620.1075569304356868No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC6320.10268274929811792No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC207.030729E-445.0000046
GTCGAAA207.030729E-445.00000411
TACGCCG850.045.00000429
CATCGCA207.030729E-445.00000411
AGTAACG207.030729E-445.0000041
CGCGCTA351.2107012E-745.041
GTTACTC453.8380676E-1045.027
GCCGATC502.1827873E-1145.032
ACGGCTA750.045.030
AATTGCG253.888686E-545.01
CTTGCGT253.888686E-545.015
CATACGA253.888686E-545.018
ATAACGG502.1827873E-1145.02
ACATACG253.888686E-545.017
CGATCGA253.888686E-545.041
GTAGTCC351.2107012E-745.033
CTATGCG302.1638534E-644.9999961
GGCGTAG302.1638534E-644.9999961
GCCGATA302.1638534E-644.9999969
ATGCGAG302.1638534E-644.9999961
Produced by FastQC (version 0.11.5)