##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551288_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	615488
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.439365186648644	31.0	31.0	34.0	30.0	34.0
2	31.593507915670166	31.0	31.0	34.0	30.0	34.0
3	31.60779901476552	31.0	31.0	34.0	30.0	34.0
4	35.41329156701674	37.0	35.0	37.0	33.0	37.0
5	34.229190495996676	35.0	35.0	37.0	32.0	37.0
6	34.628499662056775	36.0	35.0	37.0	32.0	37.0
7	35.252123518248936	37.0	35.0	37.0	32.0	37.0
8	34.50604235988354	35.0	35.0	37.0	31.0	37.0
9	36.00435589320994	39.0	35.0	39.0	31.0	39.0
10	36.111761724030366	37.0	35.0	39.0	32.0	39.0
11	36.45461162524696	38.0	35.0	39.0	32.0	39.0
12	36.42931300041593	38.0	35.0	39.0	32.0	39.0
13	36.35844078194863	39.0	35.0	39.0	31.0	39.0
14	37.455679070916084	39.0	36.0	41.0	32.0	41.0
15	37.396464269002806	39.0	36.0	41.0	31.0	41.0
16	37.75209427316211	39.0	36.0	41.0	33.0	41.0
17	37.27931007590725	39.0	36.0	41.0	31.0	41.0
18	37.29496594572112	39.0	36.0	40.0	32.0	41.0
19	37.21317881095976	39.0	36.0	40.0	32.0	41.0
20	36.58476688416346	39.0	35.0	40.0	30.0	41.0
21	36.980655999792035	39.0	35.0	40.0	31.0	41.0
22	36.85389642040137	39.0	35.0	41.0	31.0	41.0
23	36.60750493917022	39.0	35.0	41.0	30.0	41.0
24	36.751861937194555	39.0	35.0	41.0	30.0	41.0
25	36.96480028855152	39.0	35.0	40.0	32.0	41.0
26	36.71555091244671	39.0	35.0	40.0	30.0	41.0
27	36.386569681293544	39.0	35.0	41.0	30.0	41.0
28	36.18661452376001	39.0	35.0	41.0	29.0	41.0
29	36.349017365082666	39.0	35.0	41.0	30.0	41.0
30	36.0544608505771	39.0	35.0	40.0	29.0	41.0
31	35.9337550041593	39.0	35.0	40.0	27.0	41.0
32	35.2308070994073	39.0	35.0	40.0	23.0	41.0
33	34.953467817406676	39.0	35.0	41.0	21.0	41.0
34	34.52710044712488	39.0	34.0	41.0	18.0	41.0
35	34.35392241603411	39.0	34.0	41.0	15.0	41.0
36	34.42311791619008	39.0	34.0	41.0	18.0	41.0
37	34.58385541229074	39.0	34.0	41.0	19.0	41.0
38	34.34437551991265	38.0	34.0	40.0	18.0	41.0
39	34.052655778829156	38.0	34.0	40.0	14.0	41.0
40	34.33766864666736	38.0	33.0	40.0	20.0	41.0
41	33.91716654102111	38.0	33.0	40.0	18.0	41.0
42	33.75631856348134	38.0	33.0	40.0	11.0	41.0
43	33.739073710616616	38.0	34.0	40.0	11.0	41.0
44	34.10309218051368	38.0	34.0	40.0	13.0	41.0
45	34.16060914266403	38.0	34.0	40.0	15.0	41.0
46	34.0560693303525	38.0	34.0	40.0	13.0	41.0
47	33.792673455859415	38.0	33.0	40.0	13.0	41.0
48	33.86360253977332	38.0	34.0	40.0	15.0	41.0
49	33.99118423104918	38.0	34.0	40.0	15.0	41.0
50	33.6701105464282	38.0	34.0	40.0	11.0	41.0
51	33.03692517157118	37.0	32.0	40.0	11.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	10.0
9	12.0
10	22.0
11	24.0
12	19.0
13	27.0
14	23.0
15	43.0
16	79.0
17	127.0
18	288.0
19	841.0
20	2395.0
21	5276.0
22	8298.0
23	10888.0
24	12858.0
25	13705.0
26	13327.0
27	12206.0
28	10792.0
29	10383.0
30	11709.0
31	14195.0
32	17731.0
33	23379.0
34	36035.0
35	43208.0
36	46332.0
37	65590.0
38	112168.0
39	143456.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	54.73867890194447	15.505745034834147	15.676828792762814	14.078747270458564
2	27.81678927940106	43.498167307892274	15.753028491213476	12.93201492149319
3	22.578831756264947	42.72658443381512	23.598672922948943	11.095910886970989
4	21.482953363834874	17.01349173338879	47.750240459602786	13.753314443173547
5	38.47516117292295	22.41440937922429	22.408235416450037	16.702194031402726
6	19.14708329000728	44.33961344494125	24.920550847457626	11.592752417593845
7	55.568914422377034	5.01390766351253	35.021478891546224	4.395699022564209
8	52.34886789019445	24.578545804304877	16.622907351564937	6.449678953935739
9	48.06982426952272	6.595092024539877	16.37854840386815	28.956535302069252
10	44.65269834667776	15.959043880628055	24.36310699802433	15.025150774669854
11	36.58332900072788	18.570630134137463	27.200530310907766	17.645510554226888
12	22.49954507642716	16.18894275761672	31.337897733180824	29.973614432775292
13	28.66408443381512	18.37078870749714	37.940301029427054	15.024825829260685
14	17.318452999896017	35.331639284600186	27.498830196526985	19.851077518976812
15	13.681663980451283	21.207887074971403	39.787778153270246	25.32267079130706
16	15.24140194447333	23.341478631589894	27.63059555994593	33.786523863990844
17	15.64173468857232	32.34246646563378	32.49649058958095	19.519308256212955
18	14.777867318290527	23.952213528127274	31.18127404596028	30.088645107621918
19	15.987314131225954	24.98521498388271	28.343688260372257	30.68378262451908
20	26.005381095975878	23.853267651034628	35.9386373089321	14.202713944057399
21	18.44958796922117	36.10744644899657	29.535750493917025	15.90721508786524
22	15.839626442757618	19.950998232296975	39.67323489653738	24.536140428408025
23	24.937448008734535	26.249252625558906	31.62125012997816	17.192049235728398
24	20.211117032338567	28.79487496100655	27.58672792970781	23.407280076947075
25	15.341647603202663	39.80386295102423	25.9044855464282	18.95000389934491
26	15.326700114380785	21.406428719975043	40.18599875220963	23.08087241343454
27	28.342388478735575	23.810212384319435	26.08580508474576	21.761594052199232
28	13.808880108141834	31.34585889570552	34.26338125194967	20.581879744202976
29	18.978436622647397	18.952440989913693	39.051776801497354	23.017345585941563
30	14.850492617240304	34.30253717375481	28.81388426744307	22.03308594156182
31	18.53326141208277	25.75403582198191	24.349784236248308	31.36291852968701
32	26.841465633773527	26.543165748154312	24.096164344390143	22.51920427368202
33	22.190684465009877	22.206444317354684	22.85568524487886	32.747185972756576
34	18.715880732037018	26.561037745658727	28.319479827389	26.403601694915253
35	21.734948528647188	24.797234064677134	24.45539149422897	29.01242591244671
36	15.25098783404388	34.05671597171675	26.889557554330874	23.802738639908497
37	14.840744254965166	23.750584901736506	29.2239653738172	32.18470546948113
38	15.982602422792972	28.0221547779973	21.691243371113654	34.303999428096084
39	19.242617240303627	28.54742903192264	25.05719039201414	27.152763335759595
40	15.549125246958512	26.044861963190186	27.698834095871895	30.707178693979408
41	15.497458926900281	29.844611105334305	23.342453467817407	31.31547649994801
42	17.50009748362275	27.5472470624935	31.49224680253717	23.460408651346572
43	20.671402204429658	22.959180357699907	25.04500493917022	31.324412498700216
44	19.69916554018925	21.71853878548404	31.080378496412603	27.50191717791411
45	17.885970157013624	21.90180799625663	28.36854658417386	31.84367526255589
46	27.184607985858378	26.439508162628677	25.125103982530934	21.25077986898201
47	14.916943953415826	25.99108349797234	36.935082406155765	22.156890142456067
48	19.567725122179475	27.475271654362064	25.95468961214516	27.0023136113133
49	18.09718467297494	20.97945045232401	37.37765805344702	23.54570682125403
50	20.573756108973694	20.81811505667048	30.715627274617862	27.892501559737966
51	18.66600161172923	20.257259280440888	29.799443693459498	31.277295414370386
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	456.0
2	792.0
3	7472.0
4	14152.0
5	9369.0
6	4586.0
7	4278.0
8	3970.0
9	3790.0
10	3610.0
11	3391.0
12	3172.0
13	3030.5
14	2889.0
15	2685.5
16	2482.0
17	2333.5
18	2185.0
19	2169.0
20	2153.0
21	2191.0
22	2229.0
23	2148.5
24	2068.0
25	2557.0
26	3764.0
27	4482.0
28	5530.0
29	6578.0
30	7403.5
31	8229.0
32	9266.5
33	10304.0
34	11108.5
35	11913.0
36	12617.0
37	13321.0
38	15310.0
39	17299.0
40	19232.5
41	21166.0
42	22667.0
43	24168.0
44	28854.0
45	33540.0
46	79422.5
47	125305.0
48	92190.5
49	59076.0
50	57536.5
51	55997.0
52	48347.5
53	40698.0
54	37410.5
55	34123.0
56	31783.0
57	29443.0
58	26613.0
59	23783.0
60	20199.5
61	16616.0
62	14194.0
63	11772.0
64	9929.5
65	8087.0
66	6502.5
67	4918.0
68	4014.0
69	3110.0
70	2526.0
71	1942.0
72	1390.5
73	839.0
74	739.5
75	516.5
76	393.0
77	268.0
78	143.0
79	124.0
80	105.0
81	65.0
82	25.0
83	15.5
84	6.0
85	3.5
86	1.0
87	1.5
88	2.0
89	2.5
90	3.0
91	3.5
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	615488.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.322151896529707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85027208782023	22.999434716399925
2	9.070715573653695	5.500872308690899
3	3.2113218598033786	2.92122567664813
4	1.6637617347790723	2.017953441664192
5	1.1672184728006347	1.7696287914348137
6	0.8996461650398089	1.6367524601680512
7	0.7370600995570292	1.564447380694768
8	0.6494943522904633	1.5755253124871682
9	0.5557242019573244	1.5165678297894967
>10	6.0247688312683305	33.873355257224176
>50	0.09451403213809569	1.9805788958108608
>100	0.060293434294991496	3.2789492438920855
>500	0.007061393205719725	1.4979929361662907
>1k	0.004888656834729041	3.8514641912383225
>5k	0.0016295522782430135	3.350595964863205
>10k+	0.0016295522782430135	10.664655592827634
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	22193	3.6057567328688784	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	21903	3.5586396485390455	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	20654	3.355711240511594	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8642	1.4040891130290112	No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	6331	1.028614692731621	RNA PCR Primer, Index 14 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	5370	0.872478423624831	RNA PCR Primer, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCACCAACT	4995	0.8115511594052198	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	4899	0.7959537797649996	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCACCAACTCGTA	3477	0.5649175938442341	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTC	3413	0.5545193407507538	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	1734	0.2817276697514818	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCACTCGAATGC	1551	0.25199516481231155	No Hit
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	1152	0.18716855568264532	RNA PCR Primer, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1084	0.1761204117708225	RNA PCR Primer, Index 16 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATG	1079	0.17530804824789434	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCACCAAC	957	0.15548637828844755	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCCCTCGAATGC	872	0.14167619839866902	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCACCAACTCGT	806	0.13095299989601747	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	793	0.12884085473640428	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTAT	772	0.12542892794010607	No Hit
GAATGATACGGCGACCACCGAGGAATCTGTCTCTTATACACATCTGACGCT	720	0.11698034730165331	Illumina Single End PCR Primer 1 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACACCCACTCGAATGC	712	0.11568056566496829	No Hit
GAATATGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	662	0.1075569304356868	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	632	0.10268274929811792	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6247270458562963E-4	0.0	0.0	0.1952921909119268	0.0
2	1.6247270458562963E-4	0.0	0.0	1.6141663200582301	0.0
3	1.6247270458562963E-4	0.0	0.0	1.889232608921701	0.0
4	1.6247270458562963E-4	0.0	0.0	3.118013673702818	0.0
5	1.6247270458562963E-4	0.0	0.0	7.708517469065197	0.0
6	1.6247270458562963E-4	0.0	0.0	8.545089424976604	0.0
7	1.6247270458562963E-4	0.0	0.0	9.897024799833629	0.0
8	1.6247270458562963E-4	0.0	0.0	11.15050171571176	0.0
9	1.6247270458562963E-4	0.0	0.0	11.445877092648436	0.0
10	1.6247270458562963E-4	0.0	0.0	15.88219429135905	0.0
11	1.6247270458562963E-4	0.0	0.0	16.865316626806695	0.0
12	1.6247270458562963E-4	0.0	0.0	21.318206041385046	0.0
13	1.6247270458562963E-4	0.0	0.0	21.815047572007902	0.0
14	1.6247270458562963E-4	0.0	0.0	22.09255095144016	0.0
15	1.6247270458562963E-4	0.0	0.0	22.97770224602267	0.0
16	1.6247270458562963E-4	0.0	0.0	23.598185504835186	0.0
17	1.6247270458562963E-4	0.0	0.0	24.202746438598314	0.0
18	3.2494540917125925E-4	0.0	0.0	24.745080326505146	0.0
19	3.2494540917125925E-4	0.0	0.0	26.14315794946449	0.0
20	3.2494540917125925E-4	0.0	0.0	26.68630420089425	0.0
21	3.2494540917125925E-4	0.0	0.0	27.144477227825725	0.0
22	3.2494540917125925E-4	0.0	0.0	27.74026463554123	0.0
23	3.2494540917125925E-4	0.0	0.0	28.2063988249974	0.0
24	3.2494540917125925E-4	0.0	0.0	28.59942029739004	0.0
25	3.2494540917125925E-4	0.0	0.0	28.942400176770303	0.0
26	3.2494540917125925E-4	0.0	0.0	29.31949932411355	0.0
27	3.2494540917125925E-4	0.0	0.0	29.822352344806074	0.0
28	3.2494540917125925E-4	0.0	0.0	30.178005095144016	0.0
29	3.2494540917125925E-4	0.0	0.0	30.51351123011334	0.0
30	3.2494540917125925E-4	0.0	0.0	30.84934231049184	0.0
31	3.2494540917125925E-4	0.0	0.0	31.216693095559947	0.0
32	3.2494540917125925E-4	0.0	0.0	31.556261048143913	0.0
33	3.2494540917125925E-4	0.0	0.0	31.899565872933348	0.0
34	3.2494540917125925E-4	0.0	0.0	32.2189872101487	0.0
35	3.2494540917125925E-4	0.0	0.0	32.560342362483105	0.0
36	3.2494540917125925E-4	0.0	0.0	32.897148279089116	0.0
37	3.2494540917125925E-4	0.0	0.0	33.23769106790059	0.0
38	3.2494540917125925E-4	0.0	0.0	33.55093844234169	0.0
39	3.2494540917125925E-4	0.0	0.0	33.8583367994177	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	20	7.030729E-4	45.000004	6
GTCGAAA	20	7.030729E-4	45.000004	11
TACGCCG	85	0.0	45.000004	29
CATCGCA	20	7.030729E-4	45.000004	11
AGTAACG	20	7.030729E-4	45.000004	1
CGCGCTA	35	1.2107012E-7	45.0	41
GTTACTC	45	3.8380676E-10	45.0	27
GCCGATC	50	2.1827873E-11	45.0	32
ACGGCTA	75	0.0	45.0	30
AATTGCG	25	3.888686E-5	45.0	1
CTTGCGT	25	3.888686E-5	45.0	15
CATACGA	25	3.888686E-5	45.0	18
ATAACGG	50	2.1827873E-11	45.0	2
ACATACG	25	3.888686E-5	45.0	17
CGATCGA	25	3.888686E-5	45.0	41
GTAGTCC	35	1.2107012E-7	45.0	33
CTATGCG	30	2.1638534E-6	44.999996	1
GGCGTAG	30	2.1638534E-6	44.999996	1
GCCGATA	30	2.1638534E-6	44.999996	9
ATGCGAG	30	2.1638534E-6	44.999996	1
>>END_MODULE