FastQCFastQC Report
Sat 18 Jun 2016
SRR3551287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475311
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC84701.7819911594724296No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCG67461.4192812705786317No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC66891.4072891222799389No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65191.371523065950504No Hit
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC27880.5865633238027312RNA PCR Primer, Index 40 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTT21950.4618029037829968No Hit
GAACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT21150.4449718184514981RNA PCR Primer, Index 40 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAAGAAGCT20700.43550433295253005No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCT15650.3292581067974442No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTA13200.27771290796972925No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTC12110.2547805542055622No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC8620.18135494444689898No Hit
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT8600.1809341673136115Illumina PCR Primer Index 8 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCC6220.13086168845240276No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6080.12791624851939049No Hit
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC5810.12223575722000964Illumina PCR Primer Index 8 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5630.11844876302042243No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5150.10835011182152315No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA5150.10835011182152315No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG207.029108E-445.040
CGTAGGC207.029108E-445.033
CGGGATA502.1827873E-1145.06
CGTAAGG253.887341E-545.02
CGGTCTA207.029108E-445.031
GTAAGCG253.887341E-545.01
CGTTTTT25550.043.2387471
CGATGAA15250.042.49180219
CTCGAAT1450.041.8965543
CCGATGA15150.041.8811918
GCTACGA650.041.5384610
ACCGATG15400.041.347417
GATGAAT16200.041.1111120
ATGAATG16500.040.63636421
CACATAG501.0786607E-940.534
TACGGGT501.0786607E-940.54
CAGGGTA501.0786607E-940.55
CTCATGC501.0786607E-940.523
TACGGCT7800.040.3846177
TCGAGAG451.924127E-840.0000041
Produced by FastQC (version 0.11.5)