FastQCFastQC Report
Sat 18 Jun 2016
SRR3551286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622347
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99971.60633858602998No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC88361.4197867106292792No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG62341.0016919821257273No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC59410.9546121376016916No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC26240.4216297338944351TruSeq Adapter, Index 21 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT26070.4188981388196617No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT15400.24745037736182549No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTC14240.22881125802807759No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCGGTTT13910.22350874994175274No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT12520.20117394315389966No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTA9790.15730773989430333No Hit
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC7300.11729790615203416TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCC6410.1029972025252793No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT6230.10010492538728394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATGCG302.1638898E-645.0000041
CGACGGT406.8066583E-945.028
TAGCGCG207.0307904E-445.01
GCCGATC351.2107193E-745.09
CGGTCTA406.8066583E-945.031
ACGTACG253.8887363E-545.01
CGCGTAA207.0307904E-445.041
CGATCGA502.1827873E-1145.041
TGTCGAG253.8887363E-545.01
ACGGTCT406.8066583E-945.030
CGTTTTT43750.043.7142871
CCGATGA10350.041.30434818
CCGATCG556.002665E-1140.90909240
CGTTATT1600.040.781251
CGATGAA10750.040.18604319
CAATTGG451.925946E-840.02
TTCGATG451.925946E-840.01
CGGCGAA1750.039.85714331
GATGAAT11800.039.6610220
ATCCGGG403.4548066E-739.3753
Produced by FastQC (version 0.11.5)