##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551286_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	622347
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.621651586655034	31.0	31.0	34.0	30.0	34.0
2	31.774158146500266	33.0	31.0	34.0	30.0	34.0
3	31.73660674832529	33.0	31.0	34.0	30.0	34.0
4	35.4466881016539	37.0	35.0	37.0	33.0	37.0
5	34.30795842190932	37.0	35.0	37.0	32.0	37.0
6	34.758689284273885	37.0	35.0	37.0	32.0	37.0
7	35.457918171052484	37.0	35.0	37.0	33.0	37.0
8	35.20448560047691	37.0	35.0	37.0	32.0	37.0
9	37.1022998423709	39.0	37.0	39.0	34.0	39.0
10	36.68634861259073	39.0	35.0	39.0	32.0	39.0
11	36.831087801499805	39.0	37.0	39.0	33.0	39.0
12	36.89271740684859	39.0	37.0	39.0	33.0	39.0
13	37.01246571446476	39.0	37.0	39.0	33.0	39.0
14	38.10640205544495	40.0	37.0	41.0	33.0	41.0
15	38.16997591375873	40.0	37.0	41.0	33.0	41.0
16	38.28195845725937	40.0	38.0	41.0	34.0	41.0
17	38.11256581939015	40.0	37.0	41.0	33.0	41.0
18	37.9642723432426	39.0	37.0	41.0	33.0	41.0
19	37.86232439459015	39.0	37.0	41.0	33.0	41.0
20	37.76992417413437	39.0	36.0	41.0	33.0	41.0
21	37.728706011276664	39.0	36.0	41.0	33.0	41.0
22	37.804758438620254	40.0	36.0	41.0	33.0	41.0
23	37.830962469490494	40.0	36.0	41.0	33.0	41.0
24	37.74681487980178	40.0	36.0	41.0	33.0	41.0
25	37.50894436704925	39.0	35.0	41.0	33.0	41.0
26	37.5971781016057	39.0	35.0	41.0	33.0	41.0
27	37.59581712453021	40.0	35.0	41.0	33.0	41.0
28	37.440286528255136	40.0	35.0	41.0	33.0	41.0
29	37.470636156356505	40.0	35.0	41.0	33.0	41.0
30	37.19137073047673	39.0	35.0	41.0	32.0	41.0
31	37.09432358475256	39.0	35.0	41.0	32.0	41.0
32	36.90489228677892	40.0	35.0	41.0	31.0	41.0
33	36.75564917963773	40.0	35.0	41.0	31.0	41.0
34	36.44547173843531	40.0	35.0	41.0	30.0	41.0
35	36.25341489554862	40.0	35.0	41.0	29.0	41.0
36	36.152222152593325	39.0	35.0	41.0	28.0	41.0
37	36.14965123958178	40.0	35.0	41.0	28.0	41.0
38	35.98320872439331	39.0	35.0	41.0	27.0	41.0
39	35.933694546611456	39.0	35.0	41.0	27.0	41.0
40	35.80323195901965	39.0	35.0	41.0	26.0	41.0
41	35.65892018439874	39.0	35.0	41.0	25.0	41.0
42	35.718748543818805	39.0	35.0	41.0	26.0	41.0
43	35.723109455014644	39.0	35.0	41.0	26.0	41.0
44	35.899536753611734	39.0	35.0	41.0	26.0	41.0
45	35.894603814270816	39.0	35.0	41.0	26.0	41.0
46	35.806476129876096	39.0	35.0	41.0	26.0	41.0
47	35.673873257202175	39.0	35.0	41.0	26.0	41.0
48	35.651657355141104	39.0	35.0	41.0	26.0	41.0
49	35.6432440423108	39.0	35.0	41.0	26.0	41.0
50	35.53333911788761	39.0	35.0	41.0	26.0	41.0
51	34.7551590993449	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	25.0
10	27.0
11	21.0
12	30.0
13	32.0
14	36.0
15	51.0
16	72.0
17	129.0
18	253.0
19	501.0
20	1174.0
21	2227.0
22	3091.0
23	3959.0
24	5040.0
25	7255.0
26	9203.0
27	10093.0
28	9449.0
29	9065.0
30	9753.0
31	11699.0
32	15062.0
33	20565.0
34	32819.0
35	42098.0
36	47673.0
37	71029.0
38	127630.0
39	182219.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.51060421276233	20.26313294673229	21.779007531168304	16.447255309337073
2	34.1121592937702	29.46909039490831	21.895984073193894	14.522766238127605
3	28.570234933244638	28.564129014842205	28.79615391413472	14.069482137778442
4	25.592956983804854	22.275193742397732	35.48743707288699	16.644412200910423
5	27.73468820449042	28.870871073532932	26.702145266226076	16.692295455750568
6	23.640027187405096	35.022423181922626	27.324466897084744	14.013082733587531
7	70.272693529494	5.651188163516495	19.644024957138058	4.432093349851449
8	71.03609401186155	9.878251200696718	14.214738722931097	4.870916064510634
9	64.87715052856365	8.130351716968187	15.536348692931757	11.45614906153641
10	36.169371749201005	28.705850594603977	22.5690812360307	12.555696420164313
11	26.4587119404448	23.320109199530165	32.55627487559191	17.664903984433124
12	22.427681020395372	20.450648914512325	35.081393499125085	22.04027656596722
13	23.52642496870717	21.934547768367167	36.784784051341134	17.754243211584534
14	17.936456671278243	28.88485041303324	33.14919168888096	20.029501226807554
15	16.737768479642387	25.678279159375716	37.66998153763094	19.91397082335096
16	19.072318176194308	26.627106742701418	32.20663070602092	22.093944375083353
17	19.49941110023829	27.685198129018058	32.21321867061302	20.602172100130634
18	19.325392425768904	25.823857108654817	32.93355636003709	21.917194105539192
19	19.895653068143655	26.420790973524415	31.471349584717206	22.21220637361472
20	22.405667577733965	26.614894905896552	33.56230527342463	17.417132242944852
21	21.628127073802876	28.561718783893873	32.59371379632263	17.21644034598062
22	19.690944119598875	23.34758583234112	35.079786678492866	21.88168336956714
23	20.882080254263297	26.19921040834133	33.38298409086892	19.535725246526457
24	20.443900267857	26.285335994228298	32.494572963314674	20.776190774600025
25	19.01431195137118	31.133917251951083	30.03870830903017	19.813062487647567
26	18.552672383734475	26.866683698965367	32.96183640316415	21.618807514136005
27	21.951740749131915	27.284296381279255	30.98223338426955	19.781729485319282
28	18.200135937025486	28.369382354217183	33.2174815657503	20.213000143007037
29	19.68724843214477	26.58790031927526	32.46243655067029	21.262414697909687
30	19.507766567525834	30.12386980253781	31.452389101257015	18.91597452867934
31	23.19172423101582	25.87157968143174	28.368578943901074	22.56811714365137
32	23.306933270345965	27.563240443032583	28.820416905681235	20.309409380940217
33	24.069208978270964	25.86097466525909	28.444420877741837	21.625395478728105
34	23.193170369584813	25.557124883706354	29.661426824584996	21.58827792212383
35	21.61768273969345	26.528447955883134	30.802430155524167	21.05143914889925
36	21.850350367238853	29.456717876040216	30.171431693251517	18.521500063469414
37	20.60185073600419	27.75107777493906	30.81383858201293	20.83323290704382
38	22.22297207185059	26.065522931740652	28.89095633143568	22.820548664973078
39	23.33505263140981	25.655783670524645	29.328814953715533	21.680348744350017
40	22.76672017379372	24.70438517418739	31.94584371741167	20.58305093460722
41	20.64025374911424	26.51125497511838	30.924548523572863	21.923942752194513
42	20.728307519759877	26.267178921084216	32.836022347661356	20.16849121149455
43	22.10101438586512	26.033707883222707	30.191356269091035	21.673921461821138
44	21.967005545138	25.511330495688096	30.111175919543275	22.410488039630625
45	21.48029957563867	24.473967095527094	29.883650118020977	24.16208321081326
46	23.70976320284343	27.085532669073686	28.310894083204385	20.8938100448785
47	20.219588107599137	25.83847917640802	33.23162158731383	20.710311128679017
48	20.754177331938614	25.934888414341195	30.45021507294162	22.86071918077857
49	20.705329984719135	23.799584476184506	34.00835868092881	21.48672685816755
50	20.567143410348248	23.8119569950526	32.63838340989834	22.98251618470082
51	20.53709586452574	23.740614158982044	30.41791797823401	25.30437199825821
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	201.0
1	628.0
2	1055.0
3	8223.0
4	15391.0
5	10024.0
6	4657.0
7	4445.0
8	4233.0
9	4081.0
10	3929.0
11	3905.5
12	3882.0
13	3716.5
14	3551.0
15	3462.0
16	3373.0
17	3165.0
18	2957.0
19	2891.0
20	2825.0
21	2973.5
22	3122.0
23	3243.5
24	3365.0
25	3772.5
26	5565.0
27	6950.0
28	8403.0
29	9856.0
30	10617.0
31	11378.0
32	13328.0
33	15278.0
34	16911.0
35	18544.0
36	19658.5
37	20773.0
38	22482.5
39	24192.0
40	26440.0
41	28688.0
42	31593.0
43	34498.0
44	37398.5
45	40299.0
46	58955.0
47	77611.0
48	67081.5
49	56552.0
50	54795.0
51	53038.0
52	46130.0
53	39222.0
54	35289.0
55	31356.0
56	28442.0
57	25528.0
58	22768.5
59	20009.0
60	18630.0
61	17251.0
62	15280.0
63	13309.0
64	10887.5
65	8466.0
66	7108.0
67	5750.0
68	4296.0
69	2842.0
70	2320.0
71	1798.0
72	1425.0
73	1052.0
74	840.0
75	484.0
76	340.0
77	280.5
78	221.0
79	156.5
80	92.0
81	60.5
82	29.0
83	21.0
84	13.0
85	11.5
86	10.0
87	28.5
88	47.0
89	25.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	622347.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.667888777983926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.53513608020286	25.431085603781504
2	8.368440116308829	5.63495422162209
3	3.0580634773883273	3.0887562309817493
4	1.821250793441093	2.452706766015588
5	1.317945496390968	2.2186221193967963
6	0.9840328219639615	1.9878184562260992
7	0.8524544618265498	2.00902394063507
8	0.7211169131278378	1.942278722168891
9	0.6333695167709772	1.9191793001409618
>10	6.592128287299145	41.930399367833715
>50	0.07350595567720136	1.624243975946783
>100	0.03433501877002133	2.1813974327996117
>500	0.003385142695635906	0.7663709297050136
>1k	0.0029015508819736336	1.764590638653694
>5k	0.0019343672546490891	5.048572294092429
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9997	1.60633858602998	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTC	8836	1.4197867106292792	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCG	6234	1.0016919821257273	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGC	5941	0.9546121376016916	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	2624	0.4216297338944351	TruSeq Adapter, Index 21 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCT	2607	0.4188981388196617	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCT	1540	0.24745037736182549	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTC	1424	0.22881125802807759	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCGGTTT	1391	0.22350874994175274	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT	1252	0.20117394315389966	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTA	979	0.15730773989430333	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	730	0.11729790615203416	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCC	641	0.1029972025252793	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTT	623	0.10010492538728394	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.213641264439292E-4	0.0	0.0	0.10283652046205734	0.0
2	3.213641264439292E-4	0.0	0.0	0.7662927595055492	0.0
3	3.213641264439292E-4	0.0	0.0	1.0417018158679965	0.0
4	3.213641264439292E-4	0.0	0.0	1.7766615730452626	0.0
5	3.213641264439292E-4	0.0	0.0	3.9992158715314767	0.0
6	3.213641264439292E-4	0.0	0.0	4.957363014524051	0.0
7	3.213641264439292E-4	0.0	0.0	5.803996805640583	0.0
8	3.213641264439292E-4	0.0	0.0	6.813722890927409	0.0
9	3.213641264439292E-4	0.0	0.0	7.209000766453442	0.0
10	3.213641264439292E-4	0.0	0.0	8.96156003001541	0.0
11	3.213641264439292E-4	0.0	0.0	9.88580325766815	0.0
12	3.213641264439292E-4	0.0	0.0	11.693958515104917	0.0
13	3.213641264439292E-4	0.0	0.0	12.154312626235846	0.0
14	3.213641264439292E-4	0.0	0.0	12.383605930453589	0.0
15	3.213641264439292E-4	0.0	0.0	12.79414860198571	0.0
16	3.213641264439292E-4	0.0	0.0	13.271052965628499	0.0
17	3.213641264439292E-4	0.0	0.0	13.822031760416616	0.0
18	3.213641264439292E-4	0.0	0.0	14.436158606050965	0.0
19	3.213641264439292E-4	0.0	0.0	15.071013437840946	0.0
20	3.213641264439292E-4	0.0	0.0	15.51288911170135	0.0
21	3.213641264439292E-4	0.0	0.0	15.922949737043803	0.0
22	3.213641264439292E-4	0.0	0.0	16.43552551872187	0.0
23	3.213641264439292E-4	0.0	0.0	16.887363480502035	0.0
24	3.213641264439292E-4	0.0	0.0	17.261913369872435	0.0
25	3.213641264439292E-4	0.0	0.0	17.581831357747365	0.0
26	3.213641264439292E-4	0.0	0.0	17.912193679731725	0.0
27	3.213641264439292E-4	0.0	0.0	18.27244286547537	0.0
28	3.213641264439292E-4	0.0	0.0	18.581434473051207	0.0
29	3.213641264439292E-4	0.0	0.0	18.917581349311558	0.0
30	3.213641264439292E-4	0.0	0.0	19.305789214055824	0.0
31	3.213641264439292E-4	0.0	0.0	19.65880770695448	0.0
32	3.213641264439292E-4	0.0	0.0	20.01616461556013	0.0
33	3.213641264439292E-4	0.0	0.0	20.361631051487354	0.0
34	3.213641264439292E-4	0.0	0.0	20.70822226185713	0.0
35	3.213641264439292E-4	0.0	0.0	21.056902339048793	0.0
36	3.213641264439292E-4	0.0	0.0	21.396423538636807	0.0
37	3.213641264439292E-4	0.0	0.0	21.740604518058255	0.0
38	3.213641264439292E-4	0.0	0.0	22.06662842433562	0.0
39	3.213641264439292E-4	0.0	0.0	22.423985332941268	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATGCG	30	2.1638898E-6	45.000004	1
CGACGGT	40	6.8066583E-9	45.0	28
TAGCGCG	20	7.0307904E-4	45.0	1
GCCGATC	35	1.2107193E-7	45.0	9
CGGTCTA	40	6.8066583E-9	45.0	31
ACGTACG	25	3.8887363E-5	45.0	1
CGCGTAA	20	7.0307904E-4	45.0	41
CGATCGA	50	2.1827873E-11	45.0	41
TGTCGAG	25	3.8887363E-5	45.0	1
ACGGTCT	40	6.8066583E-9	45.0	30
CGTTTTT	4375	0.0	43.714287	1
CCGATGA	1035	0.0	41.304348	18
CCGATCG	55	6.002665E-11	40.909092	40
CGTTATT	160	0.0	40.78125	1
CGATGAA	1075	0.0	40.186043	19
CAATTGG	45	1.925946E-8	40.0	2
TTCGATG	45	1.925946E-8	40.0	1
CGGCGAA	175	0.0	39.857143	31
GATGAAT	1180	0.0	39.66102	20
ATCCGGG	40	3.4548066E-7	39.375	3
>>END_MODULE