SRR3551285_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551285_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541252
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	10055	1.8577298559635809	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG	9916	1.8320486575569235	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC	9289	1.7162061294923623	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8772	1.620686851965443	No Hit
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	4523	0.8356551107432398	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCT	2731	0.5045708838027388	No Hit
GAACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCT	2505	0.4628158417890373	No Hit
CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT	2341	0.4325157228056432	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTGATGCT	2255	0.4166266360216683	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTC	2008	0.37099170072350773	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTA	1976	0.3650794823852845	No Hit
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1806	0.33367082246347357	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCC	1473	0.2721468003813381	No Hit
TCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1242	0.22946797425228913	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	822	0.15187010856310923	Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTT	764	0.14115421282507962	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	737	0.1361657786022038	No Hit
CGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTG	682	0.1260041533333826	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	596	0.11011506654940767	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC	588	0.10863701196485187	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATG	558	0.10309430727276758	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	30	2.1633641E-6	45.000004	1
TTGTGCG	30	2.1633641E-6	45.000004	1
CGAAAGG	30	2.1633641E-6	45.000004	2
TAGCGAG	30	2.1633641E-6	45.000004	1
GACCGAC	30	2.1633641E-6	45.000004	35
AATCGGG	30	2.1633641E-6	45.000004	3
CGGACGA	30	2.1633641E-6	45.000004	31
CGGCGAT	85	0.0	45.0	31
GTCGATG	25	3.888062E-5	45.0	1
CGACGCT	40	6.8030204E-9	45.0	38
GCGAACG	25	3.888062E-5	45.0	1
GTTAGCG	35	1.2103374E-7	45.0	1
TGACCGC	20	7.0299755E-4	45.0	40
CTCGAAG	20	7.0299755E-4	45.0	1
TTACGAG	20	7.0299755E-4	45.0	1
GCGGACA	20	7.0299755E-4	45.0	28
TGGGCGC	20	7.0299755E-4	45.0	6
CGATCGA	20	7.0299755E-4	45.0	41
TCGAATA	20	7.0299755E-4	45.0	44
ACGACGT	20	7.0299755E-4	45.0	39

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content