##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551285_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541252
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49535151833157	31.0	31.0	34.0	30.0	34.0
2	31.64330847738207	31.0	31.0	34.0	30.0	34.0
3	31.604067975730345	31.0	31.0	34.0	30.0	34.0
4	35.37448914738421	37.0	35.0	37.0	33.0	37.0
5	34.19509581488845	37.0	35.0	37.0	32.0	37.0
6	34.67319104594533	37.0	35.0	37.0	32.0	37.0
7	35.382877107151565	37.0	35.0	37.0	33.0	37.0
8	35.07979831945194	37.0	35.0	37.0	32.0	37.0
9	36.90521790219713	39.0	37.0	39.0	32.0	39.0
10	36.44012955148434	38.0	35.0	39.0	32.0	39.0
11	36.713078196477795	39.0	35.0	39.0	32.0	39.0
12	36.793999837414	39.0	37.0	39.0	33.0	39.0
13	36.900231315542484	39.0	37.0	39.0	33.0	39.0
14	37.9547419686209	40.0	37.0	41.0	33.0	41.0
15	37.98278066408992	40.0	37.0	41.0	33.0	41.0
16	38.132348702637586	40.0	37.0	41.0	33.0	41.0
17	37.96241676705121	40.0	37.0	41.0	33.0	41.0
18	37.82064546643708	39.0	37.0	41.0	33.0	41.0
19	37.70789761515893	39.0	37.0	41.0	33.0	41.0
20	37.58790914398469	39.0	35.0	41.0	33.0	41.0
21	37.620382003207375	39.0	36.0	41.0	33.0	41.0
22	37.73191045945327	39.0	36.0	41.0	33.0	41.0
23	37.7034357378818	39.0	36.0	41.0	33.0	41.0
24	37.594094802421054	39.0	35.0	41.0	33.0	41.0
25	37.423579035273775	39.0	35.0	41.0	33.0	41.0
26	37.4997468831524	39.0	35.0	41.0	33.0	41.0
27	37.512439676897266	39.0	35.0	41.0	33.0	41.0
28	37.404257166717166	39.0	35.0	41.0	33.0	41.0
29	37.39771862274874	40.0	35.0	41.0	33.0	41.0
30	37.13541012319585	39.0	35.0	41.0	32.0	41.0
31	37.025352331261594	39.0	35.0	41.0	31.0	41.0
32	36.85933724032429	39.0	35.0	41.0	31.0	41.0
33	36.63682720802879	40.0	35.0	41.0	30.0	41.0
34	36.37152934307864	40.0	35.0	41.0	30.0	41.0
35	36.306738820364636	40.0	35.0	41.0	29.0	41.0
36	36.1610968643072	40.0	35.0	41.0	28.0	41.0
37	36.199611271644265	40.0	35.0	41.0	29.0	41.0
38	35.97324536445131	39.0	35.0	41.0	26.0	41.0
39	35.886753305299564	39.0	35.0	41.0	27.0	41.0
40	35.8099868453142	39.0	35.0	41.0	26.0	41.0
41	35.69050830297163	39.0	35.0	41.0	25.0	41.0
42	35.84618994479466	39.0	35.0	41.0	26.0	41.0
43	35.810413633575486	39.0	35.0	41.0	26.0	41.0
44	35.964744703021886	39.0	35.0	41.0	26.0	41.0
45	35.926908353225485	39.0	35.0	41.0	26.0	41.0
46	35.82145100618566	39.0	35.0	41.0	26.0	41.0
47	35.68113189420085	39.0	35.0	41.0	26.0	41.0
48	35.736876722857374	39.0	35.0	41.0	26.0	41.0
49	35.77048214140548	39.0	35.0	41.0	26.0	41.0
50	35.62043927782253	39.0	35.0	41.0	26.0	41.0
51	34.78615875784293	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	5.0
9	13.0
10	18.0
11	20.0
12	27.0
13	19.0
14	21.0
15	41.0
16	57.0
17	94.0
18	187.0
19	365.0
20	726.0
21	1585.0
22	2595.0
23	3588.0
24	4968.0
25	6802.0
26	8612.0
27	8837.0
28	8086.0
29	7845.0
30	8648.0
31	10753.0
32	13846.0
33	18364.0
34	28699.0
35	37057.0
36	43101.0
37	62913.0
38	112316.0
39	151010.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1234471189021	19.75844892951897	22.179502339021383	15.938601612557552
2	33.351377916386454	31.041917628018002	21.214702208952577	14.392002246642969
3	25.9823520282604	30.444968332680528	29.751021705231572	13.821657933827497
4	24.062913393391618	20.616829129499752	39.46479643493234	15.85546104217629
5	28.216985803285716	26.30345938675515	28.178002113618057	17.301552696341076
6	21.04121555209034	35.383702970150686	29.916563818701825	13.658517659057148
7	65.72317515685853	4.669359189434866	24.06623901620687	5.541226637499723
8	64.48678249687761	11.353861048088506	17.36751088217688	6.791845572857006
9	59.885044304686176	6.272678900031778	18.585982130320073	15.256294664961978
10	35.09049389193943	22.124999076215886	27.579020493226814	15.20548653861787
11	26.249510394418866	22.421533777242395	31.471292484831466	19.857663343507276
12	20.716043543488063	18.818590970564543	36.42037350439352	24.044991981553878
13	21.75345310502317	21.455809863058242	40.11384715437541	16.67688987754318
14	17.690096295256183	28.379202293940715	33.35248645732487	20.578214953478234
15	15.189782208656965	24.317693052404426	40.95430594251846	19.53821879642015
16	16.36280327832507	25.50715747932571	32.84181859836084	25.288220643988385
17	16.60889936665361	28.319895353735415	33.82102976062906	21.250175518981916
18	16.659707492997715	25.659766615181102	34.962087899906145	22.718437991915042
19	16.52446549851086	27.797403058094933	31.824362773717237	23.853768669676974
20	19.357341866635135	26.813572975249976	36.73556864455004	17.093516513564847
21	18.334897607768657	30.47563796531006	33.60818990045302	17.581274526468263
22	16.39384242460074	23.509936221944677	35.06961637093258	25.026604982522006
23	17.838271267357904	27.949273166658045	33.776133852623175	20.436321713360876
24	19.219698033448378	25.398520467360857	31.834339642162984	23.547441857027778
25	15.771950958148883	32.83812346189945	29.420861262406422	21.969064317545246
26	16.023220237523372	26.58299646005927	33.97807306023811	23.415710242179244
27	20.79826032975398	26.605906306119888	30.628986128457726	21.966847235668414
28	15.27255326539209	26.694589581193235	35.89344704499937	22.1394101084153
29	19.349582080066217	25.648311692150795	31.801083414010478	23.20102281377251
30	17.06303163775838	29.792222476776065	31.12376490063778	22.02098098482777
31	18.868105799147163	25.640921419228015	31.457620479924326	24.033352301700504
32	23.85654002202301	28.52589921145788	27.40368626813388	20.213874498385227
33	18.87309423337004	26.53606822699962	28.36368272080288	26.227154818827458
34	19.478542342568712	25.758611515523267	32.521450267158365	22.241395874749653
35	22.312712008454472	25.025126927937446	27.17181645518169	25.49034460842639
36	18.03614582486531	28.396569435309242	29.842106819004826	23.725177920820617
37	19.93858683201171	23.687672285737513	33.43821362322911	22.935527259021676
38	19.75715563175748	27.07740571859319	28.73375063741104	24.431688012238293
39	22.5492007419834	25.496072069941544	31.267505708985833	20.687221479089224
40	21.261630442012226	25.629281739374637	31.57180019658126	21.53728762203188
41	18.21000199537369	25.30207740571859	29.871483153872873	26.616437445034848
42	21.997886381944085	22.79067790973521	32.32228241188947	22.889153296431235
43	21.036042361044395	24.067717070791424	28.51518331571985	26.381057252444336
44	20.023944484269805	23.55852726641195	30.963580735036544	25.453947514281705
45	19.768795311610855	22.55234160797558	30.477485533540754	27.201377546872806
46	24.595752071123986	25.64812693532772	28.538277918603534	21.21784307494476
47	17.020168054806266	24.089333619090553	36.763097411187395	22.12740091491579
48	19.596047682040897	23.80203675921752	31.01013945445005	25.59177610429153
49	20.256183810868137	20.50191038555054	36.031645148655336	23.21026065492599
50	20.21553730979285	20.58542046957794	32.916460354880904	26.2825818657483
51	18.825796486664252	20.54717580720256	30.050882029073335	30.576145677059852
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	199.0
1	407.0
2	615.0
3	6724.0
4	12833.0
5	8363.0
6	3893.0
7	3741.0
8	3589.0
9	3573.5
10	3558.0
11	3529.0
12	3500.0
13	3442.5
14	3385.0
15	3269.0
16	3153.0
17	3018.5
18	2884.0
19	2841.0
20	2798.0
21	2739.5
22	2681.0
23	2671.5
24	2662.0
25	2957.0
26	3972.0
27	4692.0
28	5194.5
29	5697.0
30	6702.0
31	7707.0
32	8987.0
33	10267.0
34	11529.0
35	12791.0
36	14014.0
37	15237.0
38	16915.0
39	18593.0
40	20946.5
41	23300.0
42	26079.0
43	28858.0
44	33308.0
45	37758.0
46	64154.0
47	90550.0
48	78297.0
49	66044.0
50	61348.0
51	56652.0
52	45286.5
53	33921.0
54	28470.5
55	23020.0
56	19879.0
57	16738.0
58	14637.0
59	12536.0
60	11018.0
61	9500.0
62	8499.0
63	7498.0
64	6093.5
65	4689.0
66	3773.0
67	2857.0
68	2131.5
69	1406.0
70	1179.0
71	952.0
72	691.0
73	430.0
74	362.0
75	197.0
76	100.0
77	88.0
78	76.0
79	54.5
80	33.0
81	34.5
82	36.0
83	23.0
84	10.0
85	7.5
86	5.0
87	3.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	541252.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.34080178050774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.48110934083284	30.853092718718766
2	9.959662726987165	8.035615597402009
3	3.6163941592000515	4.376647198094257
4	1.932453470032311	3.1182688953851123
5	1.2779533676831314	2.5776831745218765
6	0.9701344408788788	2.3481600707963133
7	0.7855652376796717	2.218323207722516
8	0.6630877926552474	2.13995945652638
9	0.5209378938184639	1.8913547083037257
>10	3.676573203792075	25.0145150887717
>50	0.06616078247406665	1.8766841929987887
>100	0.03932633923259133	3.1074008084625344
>500	0.00416396533050967	1.0772969827885008
>1k	0.0046266281450107445	4.266633580482524
>5k	0.0013879884435032235	5.221680126034977
>10k+	4.6266281450107447E-4	1.8766841929900167
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	10055	1.8577298559635809	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG	9916	1.8320486575569235	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC	9289	1.7162061294923623	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8772	1.620686851965443	No Hit
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	4523	0.8356551107432398	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCT	2731	0.5045708838027388	No Hit
GAACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCT	2505	0.4628158417890373	No Hit
CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT	2341	0.4325157228056432	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTGATGCT	2255	0.4166266360216683	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTC	2008	0.37099170072350773	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTA	1976	0.3650794823852845	No Hit
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1806	0.33367082246347357	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCC	1473	0.2721468003813381	No Hit
TCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1242	0.22946797425228913	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	822	0.15187010856310923	Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTT	764	0.14115421282507962	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	737	0.1361657786022038	No Hit
CGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTG	682	0.1260041533333826	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	596	0.11011506654940767	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC	588	0.10863701196485187	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATG	558	0.10309430727276758	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.542704692084279E-4	0.0	0.0	0.44544870042050655	0.0
2	5.542704692084279E-4	0.0	0.0	2.057636738524754	0.0
3	5.542704692084279E-4	0.0	0.0	2.7018837805680165	0.0
4	5.542704692084279E-4	0.0	0.0	3.9028031305196103	0.0
5	5.542704692084279E-4	0.0	0.0	7.614013435516173	0.0
6	7.390272922779039E-4	0.0	0.0	8.989343226445353	0.0
7	7.390272922779039E-4	0.0	0.0	10.670630316377585	0.0
8	7.390272922779039E-4	0.0	0.0	12.742493330278688	0.0
9	7.390272922779039E-4	0.0	0.0	13.428495414335652	0.0
10	7.390272922779039E-4	0.0	0.0	16.748575524894136	0.0
11	7.390272922779039E-4	0.0	0.0	18.82357940478742	0.0
12	7.390272922779039E-4	0.0	0.0	22.26800085727166	0.0
13	7.390272922779039E-4	0.0	0.0	23.061900926001197	0.0
14	7.390272922779039E-4	0.0	0.0	23.39594126211081	0.0
15	7.390272922779039E-4	0.0	0.0	24.226792695454243	0.0
16	7.390272922779039E-4	0.0	0.0	25.191223311876907	0.0
17	7.390272922779039E-4	0.0	0.0	26.377177359159873	0.0
18	7.390272922779039E-4	0.0	0.0	27.63740364931677	0.0
19	7.390272922779039E-4	0.0	0.0	28.903542157811888	0.0
20	9.237841153473797E-4	0.0	0.0	29.712407529210054	0.0
21	9.237841153473797E-4	0.0	0.0	30.569124917783213	0.0
22	9.237841153473797E-4	0.0	0.0	31.53669640019806	0.0
23	9.237841153473797E-4	0.0	0.0	32.42426817822382	0.0
24	9.237841153473797E-4	0.0	0.0	33.09659825737364	0.0
25	9.237841153473797E-4	0.0	0.0	33.71738118288708	0.0
26	9.237841153473797E-4	0.0	0.0	34.2753467885569	0.0
27	9.237841153473797E-4	0.0	0.0	34.89631447089341	0.0
28	9.237841153473797E-4	0.0	0.0	35.45575813114778	0.0
29	9.237841153473797E-4	0.0	0.0	36.04513239673941	0.0
30	9.237841153473797E-4	0.0	0.0	36.69307457524406	0.0
31	9.237841153473797E-4	0.0	0.0	37.27986224531272	0.0
32	9.237841153473797E-4	0.0	0.0	37.83006806441362	0.0
33	9.237841153473797E-4	0.0	0.0	38.395793456652356	0.0
34	9.237841153473797E-4	0.0	0.0	38.918100995469764	0.0
35	9.237841153473797E-4	0.0	0.0	39.47477330337809	0.0
36	9.237841153473797E-4	0.0	0.0	39.98673446010361	0.0
37	9.237841153473797E-4	0.0	0.0	40.5321366018047	0.0
38	9.237841153473797E-4	0.0	0.0	41.063866738598655	0.0
39	9.237841153473797E-4	0.0	0.0	41.60132433690776	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	30	2.1633641E-6	45.000004	1
TTGTGCG	30	2.1633641E-6	45.000004	1
CGAAAGG	30	2.1633641E-6	45.000004	2
TAGCGAG	30	2.1633641E-6	45.000004	1
GACCGAC	30	2.1633641E-6	45.000004	35
AATCGGG	30	2.1633641E-6	45.000004	3
CGGACGA	30	2.1633641E-6	45.000004	31
CGGCGAT	85	0.0	45.0	31
GTCGATG	25	3.888062E-5	45.0	1
CGACGCT	40	6.8030204E-9	45.0	38
GCGAACG	25	3.888062E-5	45.0	1
GTTAGCG	35	1.2103374E-7	45.0	1
TGACCGC	20	7.0299755E-4	45.0	40
CTCGAAG	20	7.0299755E-4	45.0	1
TTACGAG	20	7.0299755E-4	45.0	1
GCGGACA	20	7.0299755E-4	45.0	28
TGGGCGC	20	7.0299755E-4	45.0	6
CGATCGA	20	7.0299755E-4	45.0	41
TCGAATA	20	7.0299755E-4	45.0	44
ACGACGT	20	7.0299755E-4	45.0	39
>>END_MODULE