##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551282_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	195805
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53854600240035	31.0	31.0	34.0	30.0	34.0
2	31.71318914225888	31.0	31.0	34.0	30.0	34.0
3	31.68056484768009	33.0	31.0	34.0	30.0	34.0
4	35.37362171548224	37.0	35.0	37.0	33.0	37.0
5	34.31273460841143	37.0	35.0	37.0	32.0	37.0
6	34.75389290365415	37.0	35.0	37.0	32.0	37.0
7	35.37976047598376	37.0	35.0	37.0	32.0	37.0
8	35.061229284236866	37.0	35.0	37.0	32.0	37.0
9	36.91889890452236	39.0	37.0	39.0	32.0	39.0
10	36.66313934782053	39.0	35.0	39.0	32.0	39.0
11	36.81687393069635	39.0	37.0	39.0	33.0	39.0
12	36.77292714690636	39.0	35.0	39.0	33.0	39.0
13	36.8043257322336	39.0	37.0	39.0	33.0	39.0
14	37.84038711983862	40.0	37.0	41.0	33.0	41.0
15	37.98648655550165	40.0	37.0	41.0	33.0	41.0
16	38.060790071755065	40.0	37.0	41.0	33.0	41.0
17	37.918449477796784	40.0	37.0	41.0	33.0	41.0
18	37.77480656775874	39.0	37.0	41.0	33.0	41.0
19	37.645795561911086	39.0	37.0	41.0	33.0	41.0
20	37.570465514159494	39.0	35.0	41.0	33.0	41.0
21	37.452976175276426	39.0	35.0	41.0	33.0	41.0
22	37.53590051326575	39.0	35.0	41.0	33.0	41.0
23	37.591077858073085	39.0	35.0	41.0	33.0	41.0
24	37.525962054084424	39.0	35.0	41.0	33.0	41.0
25	37.17211511452721	39.0	35.0	41.0	33.0	41.0
26	37.293250938433644	39.0	35.0	41.0	33.0	41.0
27	37.2764842572968	39.0	35.0	41.0	33.0	41.0
28	37.07749036030745	39.0	35.0	41.0	33.0	41.0
29	37.032951150379205	39.0	35.0	41.0	32.0	41.0
30	36.746155614003726	39.0	35.0	41.0	31.0	41.0
31	36.60367202063277	39.0	35.0	41.0	31.0	41.0
32	36.47678557748781	39.0	35.0	41.0	31.0	41.0
33	36.266458977043484	39.0	35.0	41.0	30.0	41.0
34	35.99137917826409	39.0	35.0	41.0	29.0	41.0
35	35.86924746559077	39.0	35.0	41.0	27.0	41.0
36	35.6690176451061	39.0	35.0	41.0	26.0	41.0
37	35.59118510763259	39.0	35.0	41.0	25.0	41.0
38	35.43057633870432	39.0	35.0	41.0	25.0	41.0
39	35.34633436326958	39.0	35.0	41.0	24.0	41.0
40	35.27774571640152	39.0	35.0	40.0	24.0	41.0
41	35.13093128367508	38.0	35.0	40.0	23.0	41.0
42	35.23011669773499	39.0	35.0	40.0	23.0	41.0
43	35.14007813896479	38.0	35.0	40.0	23.0	41.0
44	35.226873675340265	39.0	35.0	41.0	23.0	41.0
45	35.2662087280713	39.0	35.0	41.0	24.0	41.0
46	35.206200045964096	38.0	35.0	40.0	23.0	41.0
47	35.14221291591124	38.0	35.0	40.0	23.0	41.0
48	35.104588749010496	38.0	35.0	40.0	23.0	41.0
49	35.072000204284876	38.0	35.0	40.0	24.0	41.0
50	34.943341589847044	38.0	35.0	40.0	23.0	41.0
51	34.112014504226146	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	15.0
10	14.0
11	16.0
12	25.0
13	13.0
14	14.0
15	22.0
16	36.0
17	63.0
18	94.0
19	187.0
20	387.0
21	627.0
22	896.0
23	1177.0
24	1862.0
25	2418.0
26	3322.0
27	3695.0
28	3564.0
29	3348.0
30	3516.0
31	4225.0
32	5023.0
33	7126.0
34	12661.0
35	15831.0
36	15780.0
37	22393.0
38	37517.0
39	49924.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.40190495646179	20.874849978294733	18.6772554327009	19.045989632542582
2	37.41324276703863	26.7745971757616	19.808482929445113	16.003677127754653
3	29.063098490845483	25.424274150302594	30.468578432624295	15.044048926227624
4	25.64336967901739	22.22313015500115	34.753964403360484	17.379535762620975
5	26.260310002298205	29.081994841806903	27.507979877939785	17.149715277955107
6	23.27979367227599	34.10229565128572	28.948698960700696	13.669211715737594
7	67.43801230816374	6.454891345981972	20.907024846147955	5.200071499706341
8	66.84865044304283	9.936416332575778	17.012333699343735	6.202599525037665
9	60.80539312070683	9.069227037103241	18.97551135057838	11.149868491611551
10	37.22274712086004	23.576006741400885	25.398738540895277	13.8025075968438
11	28.500293659508184	23.154669186180126	30.523735348944104	17.821301805367582
12	24.92173335716657	19.45098439774265	34.736600188963514	20.890682056127268
13	24.03513699854447	23.40645029493629	35.089502310972655	17.468910395546587
14	17.03225147468144	29.254104849212226	33.06912489466561	20.64451878144072
15	16.676285079543423	23.416664538699216	40.23186333341845	19.67518704833891
16	17.923954955185007	25.428870559995914	35.161002017313145	21.486172467505938
17	18.166032532366387	25.623451903679683	32.91335767728097	23.297157886672963
18	19.83401853885243	24.0024514185031	34.36020530629963	21.803324736344834
19	20.953499655269272	24.87270498710452	31.95628303669467	22.21751232093154
20	22.52445034600751	24.523888562600547	34.4567299098593	18.49493118153265
21	21.269630499731875	25.87114731493067	34.15285615791221	18.706366027425243
22	18.540384566277673	23.476928576900487	34.31270907280202	23.669977784019817
23	19.053650315364777	24.988125941625597	33.92405709762264	22.034166645386993
24	19.95352519087868	23.78897372385792	33.36023084190904	22.89727024335436
25	18.080743596945943	28.754117617016927	31.69173412323485	21.473404662802277
26	17.641020402951916	27.03097469421108	32.7749546742933	22.553050228543704
27	20.45913025714359	26.879803886519753	31.760169556446467	20.9008962998902
28	16.627256709481372	27.80623579581727	34.76111437399453	20.805393120706825
29	17.73499144557085	26.21281376880059	35.42401879420852	20.628175991420036
30	19.201756849927225	28.283240979545976	32.366895635964354	20.148106534562444
31	20.433083935548122	28.502847220448917	28.844002962130695	22.22006588187227
32	21.755317790659074	28.362912080896812	30.872551773448077	19.00921835499604
33	23.336993437348383	27.053956742677666	28.682617910676438	20.926431909297516
34	20.785475345369118	26.766425780751256	29.388932866882868	23.059166006996755
35	22.713413855621663	26.834350501774722	28.792931743316053	21.659303899287558
36	23.50859273256556	30.025280253313248	26.91402160312556	19.552105410995633
37	20.712443502464186	29.33786164806823	30.550803094915864	19.398891754551723
38	21.527540154745793	28.45126528944613	27.491637087919102	22.52955746788897
39	23.552003268558007	24.861980031153443	29.94203416664539	21.643982533643165
40	24.163836469957356	24.087740353923547	29.935394908199488	21.813028267919616
41	20.730829141237457	26.282781338576644	28.13973085467685	24.846658665509054
42	22.74507801128674	25.63519828400705	29.579428513061462	22.04029519164475
43	24.127065192410818	25.271571206046833	28.724496310104442	21.87686729143791
44	20.756875462832923	25.94673271877633	29.47166824136258	23.824723577028166
45	20.548504890069204	25.06320063328311	29.351650877148185	25.036643599499502
46	23.432496616531754	26.999310538546002	28.94818824851255	20.620004596409693
47	20.766068282219553	25.465641837542453	32.86994714128853	20.898342738949466
48	21.22417711498685	24.081101095477646	31.581420290595236	23.113301498940274
49	22.0893235617068	22.384004494267256	34.106892060979035	21.419779883046907
50	20.653711600827354	22.582160823268048	32.80661882995838	23.95750874594622
51	20.582211894486864	22.829856234519035	29.97931615638007	26.608615714614032
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	147.0
1	246.0
2	345.0
3	3415.0
4	6485.0
5	4175.5
6	1866.0
7	1773.0
8	1680.0
9	1573.5
10	1467.0
11	1439.0
12	1411.0
13	1397.0
14	1383.0
15	1214.0
16	1045.0
17	991.5
18	938.0
19	937.5
20	937.0
21	940.0
22	943.0
23	933.5
24	924.0
25	1108.5
26	1634.5
27	1976.0
28	2140.5
29	2305.0
30	2661.5
31	3018.0
32	3345.0
33	3672.0
34	3977.0
35	4282.0
36	4624.0
37	4966.0
38	5474.5
39	5983.0
40	7060.5
41	8138.0
42	9237.5
43	10337.0
44	12002.5
45	13668.0
46	17931.5
47	22195.0
48	20757.5
49	19320.0
50	18503.5
51	17687.0
52	15222.5
53	12758.0
54	10762.5
55	8767.0
56	8191.5
57	7616.0
58	7134.0
59	6652.0
60	6489.5
61	6327.0
62	5690.5
63	5054.0
64	4366.5
65	3679.0
66	3104.0
67	2529.0
68	1979.0
69	1429.0
70	1137.0
71	845.0
72	866.0
73	887.0
74	673.0
75	345.0
76	231.0
77	160.0
78	89.0
79	56.5
80	24.0
81	22.5
82	21.0
83	21.5
84	22.0
85	11.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	195805.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.923418707387455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.88314482903404	30.70401675135977
2	8.068071482929644	6.28073848982406
3	2.674049387251686	3.1224943183269067
4	1.4472406644448526	2.253262174101785
5	0.9538929855407142	1.8564388039120554
6	0.7623271314981499	1.780342687878246
7	0.6586716351326528	1.7946426291463444
8	0.5484556643136432	1.7078215571614617
9	0.4828509197785184	1.6914787671407776
>10	5.407143044584985	38.67878756926534
>50	0.06822893431652977	1.8099639947907358
>100	0.03805075183037237	3.143433518040908
>500	0.0026241897814049914	0.7558540384566278
>1k	0.005248379562809983	4.420724700594979
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4239	2.164908965552463	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTC	1691	0.8636143101555119	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCG	1402	0.7160184877812109	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGC	1324	0.676182937105794	No Hit
GCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	908	0.46372666683690406	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTT	572	0.29212737161972374	No Hit
CCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	495	0.2528025331324532	No Hit
CTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGCT	397	0.20275273869410892	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAATGCGGT	383	0.19560276806005975	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCC	374	0.19100635836674243	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCT	331	0.1690457342764485	No Hit
GAACTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCT	330	0.16853502208830215	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	312	0.1593422027016675	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	305	0.1557672173846429	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTC	234	0.1195066520262506	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	223	0.11388881795664053	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAATGCGGTCGTA	221	0.11286739358034778	No Hit
TCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC	201	0.1026531498174204	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2206276652792319	0.0
2	0.0	0.0	0.0	1.2160057199765073	0.0
3	0.0	0.0	0.0	1.5719721151145272	0.0
4	0.0	0.0	0.0	2.190955287147928	0.0
5	0.0	0.0	0.0	4.246061132248921	0.0
6	0.0	0.0	0.0	5.2286713822425375	0.0
7	0.0	0.0	0.0	6.041214473583412	0.0
8	0.0	0.0	0.0	7.088685171471617	0.0
9	0.0	0.0	0.0	7.519726258267154	0.0
10	0.0	0.0	0.0	9.138173182503001	0.0
11	0.0	0.0	0.0	10.573274431194301	0.0
12	0.0	0.0	0.0	12.486913000178749	0.0
13	0.0	0.0	0.0	13.260131253032354	0.0
14	0.0	0.0	0.0	13.644697530706571	0.0
15	5.107121881463701E-4	0.0	0.0	14.175838206378796	0.0
16	5.107121881463701E-4	0.0	0.0	14.916370879191032	0.0
17	5.107121881463701E-4	0.0	0.0	15.744746048364444	0.0
18	5.107121881463701E-4	0.0	0.0	16.81213452159036	0.0
19	5.107121881463701E-4	0.0	0.0	17.538367253134496	0.0
20	5.107121881463701E-4	0.0	0.0	18.13998621077092	0.0
21	5.107121881463701E-4	0.0	0.0	18.724240954010366	0.0
22	5.107121881463701E-4	0.0	0.0	19.328413472587524	0.0
23	0.0010214243762927402	0.0	0.0	19.85342560200199	0.0
24	0.0010214243762927402	0.0	0.0	20.334516483235873	0.0
25	0.0010214243762927402	0.0	0.0	20.767089706595847	0.0
26	0.0010214243762927402	0.0	0.0	21.179745154618114	0.0
27	0.0010214243762927402	0.0	0.0	21.607721968284775	0.0
28	0.0010214243762927402	0.0	0.0	21.98258471438421	0.0
29	0.0010214243762927402	0.0	0.0	22.378386660197645	0.0
30	0.0010214243762927402	0.0	0.0	22.806363473864305	0.0
31	0.0010214243762927402	0.0	0.0	23.191951175914813	0.0
32	0.0010214243762927402	0.0	0.0	23.61022445800669	0.0
33	0.0010214243762927402	0.0	0.0	23.981512218789103	0.0
34	0.0010214243762927402	0.0	0.0	24.402849774009855	0.0
35	0.0010214243762927402	0.0	0.0	24.829294451112077	0.0
36	0.0010214243762927402	0.0	0.0	25.246035596639516	0.0
37	0.0010214243762927402	0.0	0.0	25.649498225275146	0.0
38	0.0010214243762927402	0.0	0.0	26.04581088327673	0.0
39	0.0010214243762927402	0.0	0.0	26.447741375347924	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTCG	35	1.2050259E-7	45.000004	40
CGGTCGA	35	1.2050259E-7	45.000004	41
GATTCAT	35	1.2050259E-7	45.000004	45
CGGGTTA	25	3.8789192E-5	45.0	6
GAAATCG	25	3.8789192E-5	45.0	7
TAGAGGC	20	7.018941E-4	45.0	4
AAATCGC	25	3.8789192E-5	45.0	8
GATCGTT	20	7.018941E-4	45.0	9
CGACGTT	20	7.018941E-4	45.0	27
ACGTTTT	25	3.8789192E-5	45.0	29
CGCGGGT	20	7.018941E-4	45.0	3
CCCTATG	20	7.018941E-4	45.0	44
TTTCGAC	20	7.018941E-4	45.0	24
AAGACGG	40	6.7648216E-9	45.0	2
CAACACA	20	7.018941E-4	45.0	26
AATGACC	20	7.018941E-4	45.0	12
AGTGAAC	25	3.8789192E-5	45.0	37
CTTGCGG	25	3.8789192E-5	45.0	2
CAAGGAT	20	7.018941E-4	45.0	4
TGTTAGG	25	3.8789192E-5	45.0	2
>>END_MODULE