SRR3551277_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551277_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	190038
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4771	2.510550521474652	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCG	4184	2.2016649301718605	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGC	3753	1.974868184257885	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTC	2583	1.359201843841758	No Hit
GCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	1016	0.5346299161220387	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTATCGCGAT	1001	0.5267367579115756	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCT	823	0.433071280480746	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTATCGCGATCGTA	654	0.3441416979761942	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTC	624	0.3283553815552679	No Hit
GAACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCT	466	0.24521411507172247	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTT	462	0.24310927288226564	No Hit
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	414	0.2178511666087835	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCC	308	0.1620728485881771	No Hit
CTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGCT	295	0.15523211147244234	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATG	260	0.13681474231469495	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	230	0.12102842589376862	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	226	0.11892358370431176	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	216	0.11366147823066966	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTATCGCGATCGT	211	0.1110304254938486	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAACCCCGATCGAATGC	204	0.10734695166229911	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	199	0.10471589892547806	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	194	0.102084846188657	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	110	0.0	45.000004	1
CGGACAT	25	3.8784812E-5	45.0	38
CGGGTCT	35	1.2047894E-7	45.0	6
CGGGTAC	25	3.8784812E-5	45.0	6
TAATACG	25	3.8784812E-5	45.0	4
ACAGTCG	25	3.8784812E-5	45.0	33
TTGTGCG	25	3.8784812E-5	45.0	1
TAGCGAG	20	7.0184126E-4	45.0	1
CCTACCT	20	7.0184126E-4	45.0	24
TACGGGA	45	3.8198777E-10	45.0	4
TGTGCGG	35	1.2047894E-7	45.0	2
CGAATGC	20	7.0184126E-4	45.0	45
GGAGTAG	20	7.0184126E-4	45.0	1
GACGGGT	35	1.2047894E-7	45.0	4
GCAATTT	20	7.0184126E-4	45.0	16
CAGTCCG	20	7.0184126E-4	45.0	1
GGCTGAC	20	7.0184126E-4	45.0	8
CGATCGA	20	7.0184126E-4	45.0	41
GGCTACG	20	7.0184126E-4	45.0	1
TCCGCTA	30	2.1559117E-6	44.999996	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content