##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551277_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	190038
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.972958039970955	33.0	31.0	34.0	30.0	34.0
2	32.158305181069046	33.0	31.0	34.0	30.0	34.0
3	32.11356149822667	33.0	31.0	34.0	30.0	34.0
4	35.76568370536419	37.0	35.0	37.0	35.0	37.0
5	34.15386396404929	37.0	35.0	37.0	33.0	37.0
6	34.84662014965428	37.0	35.0	37.0	32.0	37.0
7	35.54960060619455	37.0	35.0	37.0	33.0	37.0
8	35.244156431871524	37.0	35.0	37.0	32.0	37.0
9	37.13519927593428	39.0	37.0	39.0	34.0	39.0
10	36.92597796230228	39.0	37.0	39.0	33.0	39.0
11	37.13987728770036	39.0	37.0	39.0	34.0	39.0
12	37.14099285406077	39.0	37.0	39.0	34.0	39.0
13	37.13370483797977	39.0	37.0	39.0	33.0	39.0
14	38.29647228449047	40.0	38.0	41.0	34.0	41.0
15	38.31821004220208	40.0	38.0	41.0	34.0	41.0
16	38.526257906313475	40.0	38.0	41.0	34.0	41.0
17	38.353808185731275	40.0	38.0	41.0	34.0	41.0
18	38.16640882349846	39.0	38.0	41.0	34.0	41.0
19	38.03281975183911	39.0	37.0	41.0	34.0	41.0
20	37.74437744030141	40.0	35.0	41.0	34.0	41.0
21	37.80926446289689	39.0	35.0	41.0	34.0	41.0
22	37.83590124080447	40.0	35.0	41.0	34.0	41.0
23	37.81626832528231	40.0	35.0	41.0	34.0	41.0
24	37.75913238404951	40.0	35.0	41.0	34.0	41.0
25	37.63149475368084	39.0	35.0	41.0	34.0	41.0
26	37.67479661962344	39.0	35.0	41.0	34.0	41.0
27	37.60704174954483	40.0	35.0	41.0	33.0	41.0
28	37.46839579452531	40.0	35.0	41.0	33.0	41.0
29	37.4887601427083	40.0	35.0	41.0	33.0	41.0
30	37.21670402761553	40.0	35.0	41.0	33.0	41.0
31	37.05845672970669	39.0	35.0	41.0	33.0	41.0
32	36.75113398372957	40.0	35.0	41.0	31.0	41.0
33	36.50265736326419	40.0	35.0	41.0	30.0	41.0
34	36.21415190646081	40.0	35.0	41.0	29.0	41.0
35	36.056988602279546	40.0	35.0	41.0	26.0	41.0
36	35.88491775329145	40.0	35.0	41.0	25.0	41.0
37	35.816605100032625	40.0	35.0	41.0	24.0	41.0
38	35.66376724655069	39.0	35.0	41.0	24.0	41.0
39	35.59924330923289	39.0	35.0	41.0	23.0	41.0
40	35.515917869057766	39.0	35.0	41.0	23.0	41.0
41	35.391184920910554	39.0	35.0	41.0	23.0	41.0
42	35.478956840210905	39.0	35.0	41.0	23.0	41.0
43	35.429156274008356	39.0	35.0	41.0	23.0	41.0
44	35.57357475873246	39.0	35.0	41.0	23.0	41.0
45	35.5800366242541	39.0	35.0	41.0	23.0	41.0
46	35.49773203254086	39.0	35.0	41.0	23.0	41.0
47	35.31340047779918	39.0	35.0	41.0	23.0	41.0
48	35.34954061819215	39.0	35.0	41.0	23.0	41.0
49	35.353013607804755	39.0	35.0	41.0	23.0	41.0
50	35.23514770730065	39.0	35.0	41.0	23.0	41.0
51	34.43559182900262	38.0	34.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	6.0
11	8.0
12	13.0
13	10.0
14	18.0
15	17.0
16	15.0
17	27.0
18	49.0
19	100.0
20	231.0
21	555.0
22	924.0
23	1309.0
24	1793.0
25	2525.0
26	3241.0
27	3752.0
28	3457.0
29	2862.0
30	2771.0
31	3217.0
32	3997.0
33	5688.0
34	10396.0
35	13656.0
36	13403.0
37	19170.0
38	37174.0
39	59627.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.370273313758304	17.59700691440659	20.1964870183858	18.836232753449313
2	35.22874372493922	31.237436723181677	19.66554057609531	13.86827897578379
3	25.84483103379324	30.66491964764942	30.39918332123049	13.09106599732685
4	24.81240593986466	20.402761552952565	39.75152337953462	15.033309127648153
5	29.253622959618603	26.024268830444434	27.587640366663507	17.134467843273452
6	21.45886612151254	36.32957619002515	29.325187594060136	12.886370094402173
7	62.59853292499395	5.800418863595702	27.033014449741632	4.568033761668719
8	61.78238036603205	14.101390248266135	18.5452383207569	5.570991064944906
9	56.63288394952588	7.2222397625738015	20.088087645628768	16.056788642271545
10	38.52650522527073	19.160378450625664	27.38031341100201	14.93280291310159
11	29.48199833717467	20.814258200991382	32.093055073195885	17.610688388638064
12	23.494774729264673	18.188993780191332	34.96037634578347	23.355855144760522
13	23.533714309769625	20.56430819099338	39.456319262463296	16.445658236773696
14	17.627000915606352	28.876856207705824	33.34754101811217	20.148601858575653
15	15.241688504404383	23.394794725265474	41.790063040023576	19.57345373030657
16	15.953651374988159	24.436691609046612	34.212631157978926	25.3970258579863
17	16.275166019427694	27.072480240793944	36.482703459308134	20.169650280470222
18	17.202349003883434	23.459518622591272	36.200128395373554	23.13800397815174
19	18.01271324682432	24.739262673781035	34.62307538492301	22.624948694471634
20	20.724802407939467	24.281459497574172	38.353381955187906	16.640356139298458
21	19.47242130521264	29.667750660394237	34.46994811564003	16.389879918753092
22	16.95082036224334	22.203980256580262	37.70772161357202	23.137477767604373
23	18.63522032435618	26.192656205600983	35.74495627190352	19.42716719813932
24	18.480514423431103	24.918174259884864	33.93531819951799	22.665993117166042
25	16.45828728991044	31.113251034003724	32.3414264515518	20.08703522453404
26	15.558993464465	24.459318662583275	37.67088687525653	22.3108009976952
27	20.224902387943462	26.828318546816952	32.91394352708406	20.032835538155528
28	15.011734495206221	25.893768614698114	39.01588103431945	20.078615855776214
29	18.712047064271356	21.384144223786823	37.78191730075038	22.121891411191445
30	17.258653532451405	29.797724665593194	34.77409781201655	18.169523989938856
31	19.240888664372388	24.11149349077553	32.479819825508585	24.1677980193435
32	22.09979056820215	26.401035582357213	30.615982066744547	20.88319178269609
33	21.424662435933868	24.295667182353004	29.77667624369863	24.502994138014504
34	19.445058356749705	23.239036403245667	33.298077226659935	24.0178280133447
35	21.97507866847683	25.296519643439737	29.978741093886484	22.74966059419695
36	18.990412443827022	28.535345562466453	33.17283911638725	19.301402877319273
37	18.123217461770803	27.556593944369023	32.08884538881697	22.231343205043203
38	21.369936539007988	25.305465222744928	30.3476146875888	22.97698355065829
39	20.75058672476031	24.68506298740252	29.27046169713426	25.293888590702913
40	24.060451067681203	23.27692356265589	30.96906934402593	21.69355602563698
41	18.85517633315442	27.254022879634597	28.77792862480136	25.112872162409623
42	20.588513876172136	24.622970142813543	33.8690156705501	20.919500310464223
43	20.620086509013987	26.45734011092518	30.44654227049327	22.47603110956756
44	20.081246908513034	24.224102547911468	32.92920363295762	22.765446910617875
45	19.410328460623667	22.11136720024416	32.512971089992526	25.965333249139643
46	23.557393784401015	26.009008724570876	29.810879929277302	20.622717561750807
47	18.031656826529428	23.979940853934476	37.349372230816996	20.6390300887191
48	20.279628284869343	23.997305801997495	32.13357328534293	23.589492627790232
49	19.906545006788114	21.35046674875551	36.330102400572514	22.412885843883853
50	20.39592081583683	21.79774571401509	33.738515454803775	24.0678180153443
51	19.631863101064	21.124196213388903	31.683663267346528	27.560277418200567
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	119.0
1	259.0
2	399.0
3	3936.0
4	7473.0
5	4782.0
6	2091.0
7	1990.0
8	1889.0
9	1728.5
10	1568.0
11	1554.0
12	1540.0
13	1472.5
14	1405.0
15	1296.0
16	1187.0
17	1134.5
18	1082.0
19	984.5
20	887.0
21	927.5
22	968.0
23	1011.0
24	1054.0
25	1147.0
26	1642.5
27	2045.0
28	2213.0
29	2381.0
30	2779.5
31	3178.0
32	3472.0
33	3766.0
34	4310.0
35	4854.0
36	4943.0
37	5032.0
38	5437.0
39	5842.0
40	6768.5
41	7695.0
42	8538.5
43	9382.0
44	10743.0
45	12104.0
46	19988.0
47	27872.0
48	23131.5
49	18391.0
50	17649.0
51	16907.0
52	14350.0
53	11793.0
54	10117.5
55	8442.0
56	7621.0
57	6800.0
58	6184.0
59	5568.0
60	4991.5
61	4415.0
62	3873.5
63	3332.0
64	2907.0
65	2482.0
66	2147.0
67	1812.0
68	1438.5
69	1065.0
70	893.5
71	722.0
72	638.5
73	555.0
74	480.0
75	300.0
76	195.0
77	124.0
78	53.0
79	43.0
80	33.0
81	21.0
82	9.0
83	5.5
84	2.0
85	2.5
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	190038.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.62819015144339
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.10370552655779	31.32584009513887
2	8.289189084174124	6.735495006261906
3	3.1408255514253516	3.8281817320746376
4	1.7834708388918392	2.8983676948820762
5	1.4493129039360695	2.9441480125027626
6	1.1203357121579092	2.731032740820257
7	1.0542812366433965	2.9983476988812763
8	0.8211477936509993	2.6689398962312803
9	0.7602740613140955	2.7799703217251284
>10	4.376432799285057	26.310001157663205
>50	0.046626688598479446	1.2281754175480692
>100	0.04274113121527283	3.338279712478557
>500	0.003885557383206621	1.1055683600122082
>1k	0.007771114766413242	9.107652153779771
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4771	2.510550521474652	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCG	4184	2.2016649301718605	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGC	3753	1.974868184257885	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTC	2583	1.359201843841758	No Hit
GCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	1016	0.5346299161220387	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTATCGCGAT	1001	0.5267367579115756	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCT	823	0.433071280480746	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTATCGCGATCGTA	654	0.3441416979761942	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTC	624	0.3283553815552679	No Hit
GAACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCT	466	0.24521411507172247	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTT	462	0.24310927288226564	No Hit
CCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	414	0.2178511666087835	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCC	308	0.1620728485881771	No Hit
CTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGCT	295	0.15523211147244234	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATG	260	0.13681474231469495	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	230	0.12102842589376862	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	226	0.11892358370431176	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	216	0.11366147823066966	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTATCGCGATCGT	211	0.1110304254938486	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAACCCCGATCGAATGC	204	0.10734695166229911	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	199	0.10471589892547806	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	194	0.102084846188657	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15891558530399183	0.0
2	0.0	0.0	0.0	1.0797840431913617	0.0
3	0.0	0.0	0.0	1.4523411107252233	0.0
4	0.0	0.0	0.0	2.096422820699018	0.0
5	0.0	0.0	0.0	4.575400709331817	0.0
6	0.0	0.0	0.0	5.580989065344826	0.0
7	0.0	0.0	0.0	6.755491007061746	0.0
8	0.0	0.0	0.0	8.123638430208695	0.0
9	0.0	0.0	0.0	8.599858975573307	0.0
10	0.0	0.0	0.0	11.89288458097854	0.0
11	0.0	0.0	0.0	13.416264115597933	0.0
12	0.0	0.0	0.0	16.69455582567697	0.0
13	0.0	0.0	0.0	17.414938065018575	0.0
14	0.0	0.0	0.0	17.75066039423694	0.0
15	0.0	0.0	0.0	18.457361159347077	0.0
16	0.0	0.0	0.0	19.31350571990865	0.0
17	0.0	0.0	0.0	20.093349751102412	0.0
18	0.0	0.0	0.0	21.07631105357876	0.0
19	0.0	0.0	0.0	22.215556888622274	0.0
20	0.0	0.0	0.0	22.84964059819615	0.0
21	0.0	0.0	0.0	23.46320209642282	0.0
22	0.0	0.0	0.0	24.12570117555436	0.0
23	0.0	0.0	0.0	24.710847304223368	0.0
24	0.0	0.0	0.0	25.219692903524557	0.0
25	0.0	0.0	0.0	25.65486902619476	0.0
26	0.0	0.0	0.0	26.126353676633094	0.0
27	0.0	0.0	0.0	26.59783832707143	0.0
28	0.0	0.0	0.0	27.00670392237342	0.0
29	0.0	0.0	0.0	27.446089729422535	0.0
30	0.0	0.0	0.0	27.88494932592429	0.0
31	0.0	0.0	0.0	28.29749839505783	0.0
32	0.0	0.0	0.0	28.713730938022923	0.0
33	0.0	0.0	0.0	29.09839084814616	0.0
34	0.0	0.0	0.0	29.50304675906924	0.0
35	0.0	0.0	0.0	29.918226880939603	0.0
36	0.0	0.0	0.0	30.333407002809963	0.0
37	0.0	0.0	0.0	30.735431860996222	0.0
38	0.0	0.0	0.0	31.149033351224492	0.0
39	0.0	0.0	0.0	31.5121186289058	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	110	0.0	45.000004	1
CGGACAT	25	3.8784812E-5	45.0	38
CGGGTCT	35	1.2047894E-7	45.0	6
CGGGTAC	25	3.8784812E-5	45.0	6
TAATACG	25	3.8784812E-5	45.0	4
ACAGTCG	25	3.8784812E-5	45.0	33
TTGTGCG	25	3.8784812E-5	45.0	1
TAGCGAG	20	7.0184126E-4	45.0	1
CCTACCT	20	7.0184126E-4	45.0	24
TACGGGA	45	3.8198777E-10	45.0	4
TGTGCGG	35	1.2047894E-7	45.0	2
CGAATGC	20	7.0184126E-4	45.0	45
GGAGTAG	20	7.0184126E-4	45.0	1
GACGGGT	35	1.2047894E-7	45.0	4
GCAATTT	20	7.0184126E-4	45.0	16
CAGTCCG	20	7.0184126E-4	45.0	1
GGCTGAC	20	7.0184126E-4	45.0	8
CGATCGA	20	7.0184126E-4	45.0	41
GGCTACG	20	7.0184126E-4	45.0	1
TCCGCTA	30	2.1559117E-6	44.999996	2
>>END_MODULE