##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551276_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440784
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.503130785146467	31.0	31.0	34.0	30.0	34.0
2	31.668840066790082	31.0	31.0	34.0	30.0	34.0
3	31.649540364441542	31.0	31.0	34.0	30.0	34.0
4	35.39264356237976	37.0	35.0	37.0	33.0	37.0
5	34.25774075284039	37.0	35.0	37.0	32.0	37.0
6	34.70323786707321	37.0	35.0	37.0	32.0	37.0
7	35.33407746197684	37.0	35.0	37.0	32.0	37.0
8	34.91129215216523	37.0	35.0	37.0	32.0	37.0
9	36.70732376855784	39.0	37.0	39.0	32.0	39.0
10	36.525454644451706	38.0	35.0	39.0	32.0	39.0
11	36.732869160405095	39.0	35.0	39.0	33.0	39.0
12	36.7307524774039	39.0	35.0	39.0	33.0	39.0
13	36.756223002649826	39.0	35.0	39.0	33.0	39.0
14	37.78420723075248	40.0	37.0	41.0	33.0	41.0
15	37.84363543141312	40.0	37.0	41.0	33.0	41.0
16	38.00330093651312	40.0	37.0	41.0	33.0	41.0
17	37.80954617227486	40.0	36.0	41.0	33.0	41.0
18	37.687994573305744	39.0	36.0	41.0	33.0	41.0
19	37.58804085447748	39.0	36.0	41.0	33.0	41.0
20	37.43190315437947	39.0	35.0	41.0	32.0	41.0
21	37.329524211405136	39.0	35.0	41.0	32.0	41.0
22	37.44238220988058	39.0	35.0	41.0	33.0	41.0
23	37.43122935496751	39.0	35.0	41.0	33.0	41.0
24	37.41509446803877	39.0	35.0	41.0	33.0	41.0
25	37.12958274347526	39.0	35.0	41.0	32.0	41.0
26	37.18146302951105	39.0	35.0	41.0	32.0	41.0
27	37.12628407564703	39.0	35.0	41.0	32.0	41.0
28	36.970141838179245	39.0	35.0	41.0	32.0	41.0
29	36.96361029438455	39.0	35.0	41.0	31.0	41.0
30	36.68680351373916	39.0	35.0	41.0	31.0	41.0
31	36.63978275073505	39.0	35.0	41.0	31.0	41.0
32	36.44872999019928	39.0	35.0	41.0	30.0	41.0
33	36.24589821772115	39.0	35.0	41.0	30.0	41.0
34	35.986887001343064	39.0	35.0	41.0	28.0	41.0
35	35.911795800210534	39.0	35.0	41.0	27.0	41.0
36	35.76089195615086	39.0	35.0	41.0	26.0	41.0
37	35.70128679806889	39.0	35.0	41.0	26.0	41.0
38	35.57889578569095	39.0	35.0	41.0	25.0	41.0
39	35.44039937928781	39.0	35.0	41.0	25.0	41.0
40	35.36701196050673	39.0	35.0	40.0	25.0	41.0
41	35.171306127264145	38.0	35.0	40.0	24.0	41.0
42	35.285906475734144	39.0	35.0	40.0	24.0	41.0
43	35.26063332607354	39.0	35.0	40.0	24.0	41.0
44	35.38142037823514	39.0	35.0	41.0	24.0	41.0
45	35.40343569639551	39.0	35.0	41.0	24.0	41.0
46	35.335772169588736	39.0	35.0	40.0	24.0	41.0
47	35.187715525064434	38.0	35.0	40.0	24.0	41.0
48	35.15416167555991	38.0	35.0	40.0	24.0	41.0
49	35.16185478601764	38.0	35.0	40.0	24.0	41.0
50	35.05269020654107	38.0	35.0	40.0	24.0	41.0
51	34.12875240480598	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	27.0
10	24.0
11	25.0
12	31.0
13	17.0
14	31.0
15	35.0
16	74.0
17	108.0
18	195.0
19	412.0
20	811.0
21	1390.0
22	2271.0
23	3374.0
24	4396.0
25	5690.0
26	7081.0
27	7859.0
28	7622.0
29	7291.0
30	8042.0
31	9483.0
32	12233.0
33	16698.0
34	27963.0
35	34588.0
36	36257.0
37	51652.0
38	84533.0
39	110540.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01025445569712	18.371129623579805	20.07967621329268	16.538939707430398
2	32.52749646085157	31.405631783367816	20.260263530436674	15.806608225343933
3	27.561118370902754	30.818949871138695	27.86557588297216	13.754355874986388
4	25.39928854041889	20.60623797597009	37.51610766271008	16.47836582090094
5	28.821826563577623	27.075166067733857	26.368924461867945	17.734082906820575
6	22.26056299684199	36.39809974953719	27.20357363243675	14.137763621184071
7	64.97558895059711	6.441023267632219	23.60634687284475	4.977040908925914
8	63.47235834331555	13.621410940506006	16.378316817307343	6.527913898871103
9	57.24708700860285	8.392092271951796	18.75	15.61082071944535
10	39.026824930124505	21.088333514828125	24.959390540491487	14.925451014555883
11	30.85797125122509	22.288921557951287	28.831355040110353	18.021752150713276
12	24.604568223891974	18.79991106755236	32.65635776253222	23.939162946023448
13	24.465497840211984	22.33429525572616	35.90602199716868	17.29418490689317
14	17.880413082144543	29.81641801880286	31.830329594540636	20.47283930451196
15	16.25149733202657	22.994936295328326	39.77730589132092	20.976260481324186
16	17.858179970234854	24.379968419906348	32.228483792515156	25.533367817343645
17	17.82687211877019	26.82810628334967	32.86484990380776	22.48017169407238
18	18.95758466732005	23.49586191876293	33.050428327706996	24.49612508621003
19	20.60056626374823	24.277650731424007	30.579149878398486	24.542633126429273
20	23.67803731532905	23.720915459726307	34.144388181059206	18.45665904388544
21	21.05747940034121	28.841110385131945	31.812633852408435	18.28877636211841
22	18.838932447638754	22.301626193328254	34.496034338814475	24.36340702021852
23	20.77412065773712	25.55877708809757	32.403853134415044	21.263249119750263
24	20.734418672184106	24.61364296344695	31.107299720498023	23.544638643870922
25	18.068033322443647	30.992277396638716	29.312543105012885	21.627146175904752
26	18.072343823732258	25.91564122109695	32.57740752840393	23.434607426766853
27	23.773322080656285	24.917646738538604	29.40555918545138	21.903471995353733
28	17.879505608189046	26.22940034121021	33.51528186141058	22.37581218919017
29	20.124142437112056	24.66604958437693	33.12529492903554	22.08451304947548
30	21.178854041888997	28.301617118588695	29.914425205996586	20.60510363352572
31	21.50078042760173	24.431240698391957	26.71648698682348	27.35149188718284
32	22.686622018948054	27.210379687102982	29.057089186540345	21.045909107408615
33	21.26619841010563	27.064730117245634	27.13619369124106	24.532877781407674
34	19.341673018984356	26.63844422665069	27.539338995970812	26.48054375839413
35	21.834050237758177	25.10118334603797	26.84330647210425	26.221459944099607
36	19.24684199063487	31.45259356056481	25.096192239282733	24.204372209517587
37	20.81972122400087	25.55242477040909	30.42056880467531	23.207285200914736
38	18.675360267160332	27.13188318995245	26.185841591346325	28.00691495154089
39	20.848306653599042	25.493892700279503	29.528748774910156	24.129051871211296
40	20.506642709354242	25.268158553849506	28.660296199499076	25.56490253729718
41	17.834131910414172	27.22898290319068	26.203537333478526	28.73334785291662
42	20.16838179244256	25.05535591128535	29.13989618497949	25.63636611129261
43	21.820891865403464	26.481451232349634	27.66298232240735	24.034674579839557
44	21.325637954190714	23.72159606519293	29.362681041054124	25.590084939562235
45	19.55742495190388	22.857000254092707	28.46677737848924	29.118797415514173
46	23.180514719227556	26.86395150459182	27.573369269301974	22.382164506878652
47	18.343678536426005	24.64222839304512	34.03322262151076	22.980870449018113
48	19.928808668191223	24.486823478166176	29.347934589277287	26.23643326436531
49	19.920868271080618	22.59496714944281	33.7133380521979	23.77082652727867
50	20.032714436095684	22.932320592398998	31.451686086609314	25.583278884896004
51	19.653617191186612	21.89348978184326	28.907129115394387	29.54576391157574
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	186.0
1	449.5
2	713.0
3	6115.0
4	11517.0
5	7519.0
6	3521.0
7	3383.5
8	3246.0
9	3112.0
10	2978.0
11	2935.0
12	2892.0
13	2687.0
14	2482.0
15	2252.0
16	2022.0
17	1921.5
18	1821.0
19	1722.5
20	1624.0
21	1732.5
22	1841.0
23	1789.0
24	1737.0
25	2240.5
26	3513.5
27	4283.0
28	4957.0
29	5631.0
30	5809.5
31	5988.0
32	6602.5
33	7217.0
34	7896.0
35	8575.0
36	9146.0
37	9717.0
38	10352.5
39	10988.0
40	13238.0
41	15488.0
42	18034.5
43	20581.0
44	23422.5
45	26264.0
46	44514.5
47	62765.0
48	54188.0
49	45611.0
50	43256.5
51	40902.0
52	34834.5
53	28767.0
54	25458.5
55	22150.0
56	20437.5
57	18725.0
58	17095.5
59	15466.0
60	14741.0
61	14016.0
62	12860.0
63	11704.0
64	9998.0
65	8292.0
66	6900.0
67	5508.0
68	4825.5
69	4143.0
70	3661.5
71	3180.0
72	2714.5
73	2249.0
74	1895.5
75	1201.0
76	860.0
77	640.5
78	421.0
79	319.5
80	218.0
81	155.5
82	93.0
83	93.5
84	94.0
85	56.0
86	18.0
87	10.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	440784.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.43328264349432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.05385339681038	26.53217112782747
2	9.360649889478358	6.446358067424045
3	3.1005802454627447	3.2028946785256114
4	1.5693957087988333	2.161577840822294
5	0.9600199436939609	1.6528319032302834
6	0.7338085405425564	1.5160462129627144
7	0.583359076076576	1.4060877564434713
8	0.4856665990758965	1.3378476221187987
9	0.4555465415105346	1.4117366538988703
>10	5.553067915778575	38.52567713338959
>50	0.08563468395178071	2.0373344220401344
>100	0.04646843315212907	2.899309975222379
>500	0.003983008555896778	0.9367212455425189
>1k	0.005310678074529037	3.498418414599378
>5k	0.0026553390372645183	6.434986945952413
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC	7400	1.6788268176703327	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7291	1.6540981523830267	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC	7077	1.6055482957639118	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCG	6384	1.4483284329739736	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	3540	0.8031144506152674	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTT	2689	0.6100493665831791	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTACCAT	1779	0.40359904170750294	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCT	1647	0.3736524011760862	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCT	1594	0.36162837126574465	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTACCATCGTA	1384	0.3139859886021271	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTC	1344	0.30491124904715233	No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT	1328	0.3012813532251624	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	949	0.2152981959417765	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCC	822	0.18648589785473157	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	598	0.13566735634687282	No Hit
TCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	597	0.13544048785799848	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	596	0.1352136193691241	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	536	0.12160151003666195	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3105829612690116	0.0
2	0.0	0.0	0.0	1.5998765835420523	0.0
3	0.0	0.0	0.0	2.0132309702711533	0.0
4	0.0	0.0	0.0	2.8562742749283094	0.0
5	0.0	0.0	0.0	5.759283458564739	0.0
6	0.0	0.0	0.0	7.113234600166975	0.0
7	0.0	0.0	0.0	8.180650840320883	0.0
8	0.0	0.0	0.0	9.547079748811209	0.0
9	0.0	0.0	0.0	9.967693927184289	0.0
10	0.0	0.0	0.0	12.430578242404444	0.0
11	0.0	0.0	0.0	13.84737195542488	0.0
12	0.0	0.0	0.0	16.736315292751097	0.0
13	0.0	0.0	0.0	17.43166721115104	0.0
14	0.0	0.0	0.0	17.783993974372937	0.0
15	0.0	0.0	0.0	18.509292533304293	0.0
16	0.0	0.0	0.0	19.29924861156485	0.0
17	0.0	0.0	0.0	20.13843515191114	0.0
18	0.0	0.0	0.0	21.03252386656503	0.0
19	0.0	0.0	0.0	22.013049475480052	0.0
20	0.0	0.0	0.0	22.62446005299648	0.0
21	0.0	0.0	0.0	23.293495226686993	0.0
22	0.0	0.0	0.0	23.960715452466513	0.0
23	0.0	0.0	0.0	24.56486623833896	0.0
24	0.0	0.0	0.0	25.063523176884825	0.0
25	0.0	0.0	0.0	25.509092889034086	0.0
26	0.0	0.0	0.0	26.027941123089768	0.0
27	0.0	0.0	0.0	26.512759083814295	0.0
28	0.0	0.0	0.0	26.928609023921013	0.0
29	0.0	0.0	0.0	27.35103815020509	0.0
30	0.0	0.0	0.0	27.828596319285637	0.0
31	0.0	0.0	0.0	28.316590438854405	0.0
32	0.0	0.0	0.0	28.73584340629424	0.0
33	0.0	0.0	0.0	29.188446041598606	0.0
34	0.0	0.0	0.0	29.64195615085847	0.0
35	0.0	0.0	0.0	30.07708991251951	0.0
36	0.0	0.0	0.0	30.5029220661367	0.0
37	0.0	0.0	0.0	30.93056916766489	0.0
38	0.0	0.0	0.0	31.342108606482995	0.0
39	0.0	0.0	0.0	31.763176521833824	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAA	35	1.2096461E-7	45.000004	45
GACGTGA	20	7.0285477E-4	45.000004	10
CACGCTA	20	7.0285477E-4	45.000004	11
ACGATAG	20	7.0285477E-4	45.000004	1
GTGCTAA	20	7.0285477E-4	45.000004	19
GTATGCG	20	7.0285477E-4	45.000004	1
CGAATTG	20	7.0285477E-4	45.000004	22
CGCAGTA	20	7.0285477E-4	45.000004	40
TATAAGC	20	7.0285477E-4	45.000004	26
TAAGACG	20	7.0285477E-4	45.000004	1
ACGAACG	20	7.0285477E-4	45.000004	1
TTTAGCG	25	3.8868784E-5	45.0	1
CGCGGGC	45	3.8380676E-10	45.0	4
GAATATA	50	2.1827873E-11	45.0	15
CGAATAT	50	2.1827873E-11	45.0	14
CGACTGG	25	3.8868784E-5	45.0	2
TCGACGT	30	2.1624455E-6	44.999996	26
CGTTTTT	3280	0.0	42.667683	1
GCGAACA	90	0.0	42.5	33
CCGATGA	885	0.0	42.45763	18
>>END_MODULE