##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551275_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	476037
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49909985988484	31.0	31.0	34.0	30.0	34.0
2	31.680306362740712	31.0	31.0	34.0	30.0	34.0
3	31.629774576345955	31.0	31.0	34.0	30.0	34.0
4	35.394813848503375	37.0	35.0	37.0	33.0	37.0
5	34.212670443684	37.0	35.0	37.0	32.0	37.0
6	34.7057602665339	37.0	35.0	37.0	32.0	37.0
7	35.36811424322059	37.0	35.0	37.0	32.0	37.0
8	34.96020897535276	37.0	35.0	37.0	32.0	37.0
9	36.83690763533087	39.0	37.0	39.0	32.0	39.0
10	36.67567016849531	39.0	35.0	39.0	32.0	39.0
11	36.86783380283465	39.0	37.0	39.0	33.0	39.0
12	36.832855429304864	39.0	35.0	39.0	33.0	39.0
13	36.848513876022245	39.0	37.0	39.0	33.0	39.0
14	37.851851851851855	40.0	37.0	41.0	33.0	41.0
15	37.933406436894614	40.0	37.0	41.0	33.0	41.0
16	38.05422477664551	40.0	37.0	41.0	33.0	41.0
17	37.895277047792504	40.0	37.0	41.0	33.0	41.0
18	37.67151292861689	39.0	36.0	40.0	33.0	41.0
19	37.50062495142184	39.0	37.0	40.0	33.0	41.0
20	37.3131584309623	39.0	35.0	41.0	33.0	41.0
21	37.213979165485036	39.0	35.0	40.0	33.0	41.0
22	37.32572047971061	39.0	35.0	41.0	33.0	41.0
23	37.3604677787651	39.0	35.0	41.0	33.0	41.0
24	37.22403720719188	39.0	35.0	41.0	33.0	41.0
25	36.950228658696695	39.0	35.0	40.0	32.0	41.0
26	37.08445562004634	39.0	35.0	41.0	33.0	41.0
27	37.08305866980928	39.0	35.0	41.0	33.0	41.0
28	36.89173530628922	39.0	35.0	41.0	32.0	41.0
29	36.8753605286984	39.0	35.0	41.0	32.0	41.0
30	36.53062261967032	39.0	35.0	41.0	31.0	41.0
31	36.31954869054296	39.0	35.0	40.0	30.0	41.0
32	36.0673435048116	39.0	35.0	41.0	30.0	41.0
33	35.709673827874724	39.0	35.0	41.0	26.0	41.0
34	35.367601678020826	39.0	35.0	41.0	23.0	41.0
35	35.20038988566015	39.0	35.0	41.0	22.0	41.0
36	34.94447910561574	39.0	35.0	41.0	21.0	41.0
37	34.978772658427815	39.0	35.0	41.0	21.0	41.0
38	34.80299430506452	38.0	35.0	40.0	20.0	41.0
39	34.71921720370475	38.0	35.0	40.0	20.0	41.0
40	34.68257719462983	38.0	35.0	40.0	20.0	41.0
41	34.42072149853898	38.0	34.0	40.0	19.0	41.0
42	34.55187727004414	38.0	35.0	40.0	18.0	41.0
43	34.44908904139804	38.0	34.0	40.0	18.0	41.0
44	34.57774080586173	38.0	35.0	40.0	18.0	41.0
45	34.58513098771734	38.0	34.0	40.0	20.0	41.0
46	34.50788279062342	38.0	34.0	40.0	20.0	41.0
47	34.3910767440346	38.0	34.0	40.0	20.0	41.0
48	34.39901310192275	38.0	34.0	40.0	20.0	41.0
49	34.39948785493564	38.0	34.0	40.0	20.0	41.0
50	34.261664534479465	38.0	34.0	40.0	19.0	41.0
51	33.47883882975483	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	21.0
10	21.0
11	16.0
12	10.0
13	23.0
14	33.0
15	30.0
16	71.0
17	119.0
18	211.0
19	447.0
20	833.0
21	1464.0
22	2277.0
23	3280.0
24	4883.0
25	7209.0
26	10310.0
27	11658.0
28	10813.0
29	9816.0
30	9743.0
31	11201.0
32	13721.0
33	18484.0
34	31016.0
35	38035.0
36	37948.0
37	52101.0
38	87778.0
39	112427.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64655268393003	18.202156555057694	18.710940536134796	21.440350224877477
2	38.59678974533492	28.374685161027397	18.708839859086584	14.319685234551097
3	26.272537638881012	26.448154240111588	33.65641746334844	13.622890657658965
4	24.556074422786462	20.13477943941332	38.85706363160847	16.452082506191744
5	25.372817659131535	26.339549236718995	31.212069650048214	17.075563454101257
6	21.048573955385823	33.55495476191977	32.018099433447404	13.378371849247012
7	61.21582986196451	5.858578219760228	27.652682459556715	5.272909458718545
8	58.76917130391125	11.480620203891714	22.771549270329828	6.978659221867208
9	53.461390606192374	8.101471104136863	24.859622256253193	13.57751603341757
10	35.083617449904104	21.132390969609503	30.154168688568326	13.629822891918064
11	27.840693055371744	21.21452744219462	33.71901763938517	17.22576186304846
12	24.15484510657785	17.246558565825765	38.55750708453334	20.04108924306304
13	22.50476328520682	20.342326331776732	40.4943313229854	16.658579060031048
14	15.896033291529859	27.349974896909274	36.6118600024788	20.142131809082066
15	14.819436304320883	21.955646304804038	45.60800946144942	17.616907929425654
16	16.433596548167476	23.008925776777854	38.27139487056678	22.286082804487886
17	16.51762363009598	23.322976995485647	38.21173564239754	21.947663732020832
18	16.82558288536395	22.709159161997913	39.132882528038785	21.33237542459935
19	19.25312528227848	23.530313820144233	35.93649233147843	21.280068566098855
20	21.354642601310402	23.3423032243292	38.67178391595611	16.631270258404285
21	19.58419198507679	25.995458336221766	37.17526158680943	17.245088091892015
22	16.271004144635818	22.440062432121874	37.49120341486061	23.797730008381702
23	17.640015376955994	24.915080130326004	38.007759901016094	19.437144591701905
24	20.113772668931198	21.6766763928014	35.85645653594153	22.353094402325873
25	17.4948585929245	27.10692656243107	34.07214145118972	21.32607339345471
26	17.35222262135086	22.666935553328837	37.13366818125482	22.84717364406548
27	19.563605350004305	23.836172398363992	35.24810046277915	21.352121788852546
28	15.29271884328319	23.631776521572903	39.7307352159601	21.344769419183805
29	20.70238237784038	20.899425884962724	36.91771858069856	21.480473156498338
30	18.496461409512285	23.306591714509587	35.32771612290641	22.869230753071715
31	19.428531815804234	22.129372296691223	36.004134132430885	22.43796175507366
32	22.72596457838361	23.64312017763325	34.08327503954525	19.54764020443789
33	18.964912391263706	21.52542764533009	33.965216989435696	25.54444297397051
34	20.682215878177537	21.259061795616727	36.462291796646056	21.596430529559676
35	21.204234124658377	21.50063965616118	32.64830254791119	24.64682367126925
36	21.143524557965033	22.937082621728983	34.89245583851675	21.02693698178923
37	18.491629852301397	22.864819331270468	38.00355854691968	20.63999226950846
38	19.25648636555562	23.40889468675754	33.094276285246735	24.240342662440103
39	20.599239134773136	21.366406392780394	37.30655390232272	20.72780057012375
40	22.18020868125797	20.406607049452038	34.58722746341146	22.825956805878533
41	18.539315221295823	23.328438755811	34.16226049655804	23.96998552633514
42	21.775828349477035	21.363465444912897	35.80330940662175	21.057396798988314
43	21.529208864016873	21.276917550526534	33.94126927108607	23.252604314370522
44	19.639229723739962	21.777718958820426	34.2922924058424	24.29075891159721
45	19.29807977111023	19.767160955976113	33.92362358388109	27.01113568903257
46	22.492999493736832	22.752853244600736	33.2243081945311	21.529839067131338
47	17.340668897585694	20.462274991229673	39.813711959364504	22.383344151820133
48	19.456680888250283	20.68725750309325	35.051056955656804	24.805004652999664
49	19.182962668868175	18.767028613322072	39.44168205412604	22.608326663683705
50	19.101456399397527	18.494360732464074	37.06791698964576	25.336265878492636
51	18.608217428477197	18.837401294437196	33.70578337398143	28.848597903104174
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	273.0
1	508.0
2	743.0
3	11487.0
4	22231.0
5	14372.5
6	6514.0
7	6102.5
8	5691.0
9	5464.5
10	5238.0
11	5089.0
12	4940.0
13	4659.5
14	4379.0
15	4078.0
16	3777.0
17	3463.0
18	3149.0
19	2924.0
20	2699.0
21	2613.0
22	2527.0
23	2639.5
24	2752.0
25	3061.5
26	4078.5
27	4786.0
28	5252.5
29	5719.0
30	6170.5
31	6622.0
32	6832.5
33	7043.0
34	8324.0
35	9605.0
36	10217.0
37	10829.0
38	11577.5
39	12326.0
40	14001.0
41	15676.0
42	18072.0
43	20468.0
44	24703.5
45	28939.0
46	45860.0
47	62781.0
48	55940.5
49	49100.0
50	46928.0
51	44756.0
52	37038.0
53	29320.0
54	25427.0
55	21534.0
56	19262.0
57	16990.0
58	16113.0
59	15236.0
60	14661.0
61	14086.0
62	12832.0
63	11578.0
64	9973.5
65	8369.0
66	6453.5
67	4538.0
68	3706.5
69	2875.0
70	2420.5
71	1966.0
72	1564.5
73	1163.0
74	931.0
75	568.5
76	438.0
77	290.0
78	142.0
79	132.0
80	122.0
81	74.0
82	26.0
83	22.0
84	18.0
85	10.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	476037.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.96947940142769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.37284344708743	27.470914191984736
2	10.23091179339092	7.360011420203967
3	3.734770631429303	4.030132658887602
4	1.8431130163912723	2.6518326271035653
5	1.0817369038773688	1.9454756640890591
6	0.7301039623816475	1.5756875661472443
7	0.5705436267311808	1.4365510060526137
8	0.4109252320419265	1.1824613335567173
9	0.3565875829757903	1.154364274858732
>10	4.491685370636078	34.02004809753409
>50	0.11569884156892092	2.7635738935865106
>100	0.05109542749490416	3.531890531025292
>500	0.002349215057236973	0.6253003153199871
>1k	0.0052857338787831885	3.23402788082219
>5k	0.0017619112929277297	4.016920775611336
>10k+	5.873037643092432E-4	3.000807763216357
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14205	2.9840117469860536	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC	6398	1.3440131754464464	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC	6311	1.3257372851269964	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG	6306	1.32468694660289	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	3953	0.8303976371584562	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCT	1631	0.3426204265634814	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTCTGTT	1618	0.339889546400805	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	1592	0.3344277860754521	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT	1491	0.31321094788850445	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTA	1368	0.287372620195489	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTC	1327	0.2787598442978172	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1287	0.27035713610496664	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTT	1042	0.218890548423757	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC	887	0.18633005417646106	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	866	0.18191863237521452	No Hit
ACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	638	0.13402319567596635	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	569	0.11952852404329915	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	482	0.10125263372384921	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1006770482126389E-4	0.0	0.0	0.34451103590687276	0.0
2	2.1006770482126389E-4	0.0	0.0	1.813514495721971	0.0
3	2.1006770482126389E-4	0.0	0.0	2.298980961563912	0.0
4	2.1006770482126389E-4	0.0	0.0	3.244495700964421	0.0
5	2.1006770482126389E-4	0.0	0.0	6.164436797979989	0.0
6	2.1006770482126389E-4	0.0	0.0	7.297121862376244	0.0
7	2.1006770482126389E-4	0.0	0.0	8.553326737207401	0.0
8	2.1006770482126389E-4	0.0	0.0	10.244791896428218	0.0
9	2.1006770482126389E-4	0.0	0.0	10.784875965523689	0.0
10	2.1006770482126389E-4	0.0	0.0	13.39958868743396	0.0
11	2.1006770482126389E-4	0.0	0.0	15.11668210664297	0.0
12	2.1006770482126389E-4	0.0	0.0	17.979064652537513	0.0
13	2.1006770482126389E-4	0.0	0.0	18.750643332346016	0.0
14	2.1006770482126389E-4	0.0	0.0	19.121622899060366	0.0
15	2.1006770482126389E-4	0.0	0.0	19.94130708327294	0.0
16	2.1006770482126389E-4	0.0	2.1006770482126389E-4	20.87904931759506	0.0
17	2.1006770482126389E-4	0.0	2.1006770482126389E-4	21.893046128767303	0.0
18	2.1006770482126389E-4	0.0	2.1006770482126389E-4	23.14925100359846	0.0
19	2.1006770482126389E-4	0.0	2.1006770482126389E-4	24.19958952770478	0.0
20	2.1006770482126389E-4	0.0	2.1006770482126389E-4	25.021374388965565	0.0
21	2.1006770482126389E-4	0.0	2.1006770482126389E-4	25.791692662545138	0.0
22	2.1006770482126389E-4	0.0	2.1006770482126389E-4	26.578816352510415	0.0
23	2.1006770482126389E-4	0.0	2.1006770482126389E-4	27.333169480523573	0.0
24	2.1006770482126389E-4	0.0	2.1006770482126389E-4	27.935223522541314	0.0
25	2.1006770482126389E-4	0.0	2.1006770482126389E-4	28.463963935576437	0.0
26	2.1006770482126389E-4	0.0	2.1006770482126389E-4	28.991864077792272	0.0
27	2.1006770482126389E-4	0.0	2.1006770482126389E-4	29.510731308700795	0.0
28	2.1006770482126389E-4	0.0	2.1006770482126389E-4	29.990525946512562	0.0
29	2.1006770482126389E-4	0.0	2.1006770482126389E-4	30.49384816726431	0.0
30	2.1006770482126389E-4	0.0	2.1006770482126389E-4	31.049057111106908	0.0
31	2.1006770482126389E-4	0.0	2.1006770482126389E-4	31.572125696111858	0.0
32	2.1006770482126389E-4	0.0	2.1006770482126389E-4	32.09456407800234	0.0
33	2.1006770482126389E-4	0.0	2.1006770482126389E-4	32.61448164743497	0.0
34	2.1006770482126389E-4	0.0	2.1006770482126389E-4	33.12095488375903	0.0
35	2.1006770482126389E-4	0.0	2.1006770482126389E-4	33.65053556761344	0.0
36	2.1006770482126389E-4	0.0	2.1006770482126389E-4	34.121507361822715	0.0
37	2.1006770482126389E-4	0.0	2.1006770482126389E-4	34.59121874980306	0.0
38	2.1006770482126389E-4	0.0	2.1006770482126389E-4	35.05924959614484	0.0
39	2.1006770482126389E-4	0.0	2.1006770482126389E-4	35.58021750410157	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	35	1.209919E-7	45.000004	4
CGACGGG	70	0.0	45.000004	3
TACGGGA	95	0.0	45.000004	4
CGGCGTA	20	7.0291176E-4	45.0	36
GTACCGC	20	7.0291176E-4	45.0	31
GTACAAT	20	7.0291176E-4	45.0	9
GTTTACG	20	7.0291176E-4	45.0	1
AAGCGTG	20	7.0291176E-4	45.0	1
TCGATTA	20	7.0291176E-4	45.0	41
TAACGGG	90	0.0	45.0	3
TATTGCG	25	3.8873506E-5	45.0	1
TCTACGG	20	7.0291176E-4	45.0	2
AGACACG	25	3.8873506E-5	45.0	24
CATACGG	25	3.8873506E-5	45.0	2
TCATCGA	25	3.8873506E-5	45.0	16
TAGGCGA	20	7.0291176E-4	45.0	19
TCATACA	20	7.0291176E-4	45.0	16
GAACCAC	20	7.0291176E-4	45.0	16
CGATCGA	55	1.8189894E-12	45.0	41
TCGACAC	20	7.0291176E-4	45.0	34
>>END_MODULE