FastQCFastQC Report
Sat 18 Jun 2016
SRR3551274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746267
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77221.0347502971456597No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC61910.8295958417027686No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC50580.6777735046571803No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG48940.655797455870352No Hit
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC27330.3662228130146449TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT16050.21507047745645994No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT12880.17259238315509062No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTATCTGT12510.16763437214830618No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT11170.14967833228589766No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT11020.14766832782368777TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTC10170.13627830253783163No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTA9850.1319902930184505No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC8320.11148824750390947No Hit
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC7680.1029122284651472TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG302.1644737E-645.0000041
GTAACGG650.045.0000042
TTCTACG207.0316956E-445.01
TCGGACA253.889486E-545.013
ACGGCTA406.8102963E-945.030
CGTAAGC207.0316956E-445.043
TTTGCGC207.0316956E-445.019
AGAATCG207.0316956E-445.026
CGTTTTT37950.042.8063241
CGAACAC700.041.78571334
CACGACG556.002665E-1140.9090926
CGATGAA8200.040.6097619
CCGATGA8300.039.84939618
CGAATAT850.039.70588314
CCCGTCG356.2453437E-638.57142640
TAATACG659.094947E-1238.0769274
GCGACTA301.1395379E-437.50000433
CGACGGT601.546141E-1037.50000428
TATTGCG301.1395379E-437.5000041
GTCGTTT301.1395379E-437.5000049