##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551274_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	746267
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56921852366512	31.0	31.0	34.0	30.0	34.0
2	31.713981725039428	33.0	31.0	34.0	30.0	34.0
3	31.681876593766038	33.0	31.0	34.0	30.0	34.0
4	35.4191690105552	37.0	35.0	37.0	33.0	37.0
5	34.25132827794878	37.0	35.0	37.0	32.0	37.0
6	34.714300645747436	37.0	35.0	37.0	32.0	37.0
7	35.42536920431964	37.0	35.0	37.0	32.0	37.0
8	35.18857191862966	37.0	35.0	37.0	32.0	37.0
9	37.07988159733715	39.0	37.0	39.0	33.0	39.0
10	36.63892547841456	39.0	35.0	39.0	32.0	39.0
11	36.78011489185506	39.0	37.0	39.0	33.0	39.0
12	36.840484705876044	39.0	37.0	39.0	33.0	39.0
13	36.9620872958338	39.0	37.0	39.0	33.0	39.0
14	38.0264824787911	40.0	37.0	41.0	33.0	41.0
15	38.12395563518151	40.0	37.0	41.0	33.0	41.0
16	38.20098302618232	40.0	37.0	41.0	34.0	41.0
17	38.0679381508227	40.0	37.0	41.0	33.0	41.0
18	37.94411383593272	39.0	37.0	41.0	33.0	41.0
19	37.867394645616116	39.0	37.0	41.0	33.0	41.0
20	37.86922776968565	40.0	36.0	41.0	33.0	41.0
21	37.75173228884568	39.0	36.0	41.0	33.0	41.0
22	37.85824242529819	40.0	36.0	41.0	33.0	41.0
23	37.913581868151745	40.0	36.0	41.0	34.0	41.0
24	37.8052707677011	40.0	36.0	41.0	33.0	41.0
25	37.50010251022757	39.0	35.0	41.0	33.0	41.0
26	37.640465141832614	39.0	36.0	41.0	33.0	41.0
27	37.67505865863022	40.0	36.0	41.0	33.0	41.0
28	37.50957499125648	40.0	36.0	41.0	33.0	41.0
29	37.509805471768146	40.0	36.0	41.0	33.0	41.0
30	37.231062072957805	40.0	35.0	41.0	32.0	41.0
31	37.1446305410798	39.0	35.0	41.0	32.0	41.0
32	37.10038096284574	40.0	35.0	41.0	32.0	41.0
33	37.00227800505717	40.0	36.0	41.0	31.0	41.0
34	36.82889770015289	40.0	35.0	41.0	31.0	41.0
35	36.760529408375284	40.0	35.0	41.0	31.0	41.0
36	36.58467947798844	40.0	35.0	41.0	30.0	41.0
37	36.59398043863657	40.0	35.0	41.0	30.0	41.0
38	36.38971306516301	40.0	35.0	41.0	30.0	41.0
39	36.325032461572064	39.0	35.0	41.0	30.0	41.0
40	36.238773790077815	39.0	35.0	41.0	29.0	41.0
41	36.10596877525068	39.0	35.0	41.0	29.0	41.0
42	36.19863132096153	39.0	35.0	41.0	29.0	41.0
43	36.12163073002022	39.0	35.0	41.0	29.0	41.0
44	36.21060424754143	39.0	35.0	41.0	29.0	41.0
45	36.198782741297684	39.0	35.0	41.0	29.0	41.0
46	36.12092856846142	39.0	35.0	41.0	29.0	41.0
47	36.03126226940224	39.0	35.0	41.0	29.0	41.0
48	35.98620198936842	39.0	35.0	41.0	29.0	41.0
49	35.95783412639176	39.0	35.0	41.0	28.0	41.0
50	35.82734061669617	39.0	35.0	41.0	28.0	41.0
51	34.95427775849662	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	6.0
8	10.0
9	19.0
10	47.0
11	23.0
12	38.0
13	36.0
14	45.0
15	59.0
16	126.0
17	142.0
18	296.0
19	565.0
20	1128.0
21	1917.0
22	2891.0
23	4039.0
24	5237.0
25	7052.0
26	9241.0
27	10558.0
28	10416.0
29	10744.0
30	11613.0
31	14216.0
32	18104.0
33	25182.0
34	41134.0
35	51064.0
36	59355.0
37	88505.0
38	154521.0
39	217877.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80550124821277	19.02295023094951	22.31319353528965	14.858354985548067
2	34.109909723999586	27.078646114594374	23.150561394246296	15.660882767159743
3	29.531789560572825	26.888365692171835	28.994314367377893	14.585530379877444
4	27.708179512158516	23.712290641285225	31.88001077362392	16.699519072932343
5	26.52589488748665	30.230199111042026	25.484042574574516	17.759863426896807
6	24.9693474319513	35.31189239240111	25.92637755655818	13.792382619089413
7	73.37561489386506	6.017149358071575	15.989987497772246	4.617248250291116
8	73.73178768456866	8.776885484685776	12.420755574077374	5.07057125666819
9	67.44141171993402	8.169194135610981	14.365367891116717	10.024026253338283
10	37.11446439411096	28.435265126288577	20.900696399546007	13.549574080054457
11	27.624027325340663	24.930621345979386	29.740294023452734	17.705057305227218
12	24.498470386604257	21.50972775159561	33.33337800009916	20.65842386170097
13	24.36487209001604	22.772814555648313	34.350574258274854	18.511739096060793
14	18.88854793257641	29.007982397720923	31.60209415664903	20.501375513053638
15	18.189870381512247	25.51982065400185	36.58904922768929	19.701259736796615
16	20.25441296479678	26.249586274081526	31.951164931586145	21.54483582953554
17	20.493067494609836	26.924277771896655	30.842982471421088	21.73967226207242
18	21.035768699406514	24.99735349412476	31.78634456568494	22.180533240783795
19	22.45724385508136	25.845307376582376	29.9774745499935	21.719974218342763
20	24.497666384819375	25.24136803583704	31.697234367860293	18.56373121148329
21	22.958136967064068	27.315424640242703	30.903148604989905	18.823289787703327
22	20.906056411445235	24.046755383796953	32.30117370860563	22.74601449615218
23	21.518839771824293	26.303856394561198	31.659178283375788	20.51812555023872
24	20.729980020555647	25.40685840322566	31.372953647957097	22.4902079282616
25	20.33132913555068	29.089320578291684	29.528707553730772	21.050642732426866
26	20.64140582392093	25.701525057385627	31.46072384280693	22.19634527588651
27	22.314131537372013	25.696701046676324	30.21666508099648	21.772502334955185
28	19.828292018808284	27.270132539694238	32.26097361936143	20.640601822136045
29	22.40444773787398	24.674144776601402	31.353925605714846	21.567481879809776
30	21.020224664898755	25.979441674360515	30.9101166204589	22.09021704028183
31	21.588519926514238	25.71184308029164	29.470417424326683	23.229219568867443
32	22.809932638050455	27.90582995094249	29.424589322588297	19.85964808841876
33	21.656458077336932	25.546486713200505	29.85352427482389	22.943530934638677
34	21.7883143700579	24.921509325750705	30.44004357689674	22.850132727294657
35	21.522725780451232	25.08539168956955	29.63805179647499	23.753830733504227
36	22.539252037139523	26.657081178720215	29.661769849129065	21.141896935011196
37	20.742576048518828	26.782371456864634	29.961796515188265	22.513255979428273
38	20.109022642030265	26.578825004991515	30.389927465638973	22.922224887339247
39	22.65878030249227	25.668024983015464	29.030762448292634	22.64243226619963
40	21.769420328113128	24.447148272669164	32.05648916540595	21.72694223381176
41	20.965418543229166	25.40779640530802	29.317121084008807	24.309663967454007
42	21.77223433436022	25.425082443683024	31.088471016405656	21.714212205551096
43	23.572930331905336	25.1723578826345	29.168782754697713	22.08592903076245
44	21.510799753975455	25.096513714260443	29.815870191231824	23.576816340532275
45	21.155832965949184	23.94893516663607	29.743108029699826	25.152123837714917
46	22.612818200456406	25.98935769637409	29.152836719297515	22.24498738387199
47	20.644755831357944	24.991323480738128	32.32422175977231	22.03969892813162
48	20.819224218677764	24.349730056400727	30.4805116667359	24.35053405818561
49	21.089502818696257	24.006957295445197	32.66846852400012	22.235071361858424
50	20.715775989022696	23.968633210365727	31.514055961204235	23.801534839407342
51	20.762274092248482	23.16329142250696	29.86759430605936	26.2068401791852
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	290.0
1	681.0
2	1072.0
3	7032.0
4	12992.0
5	8736.0
6	4480.0
7	4303.5
8	4127.0
9	3999.5
10	3872.0
11	3803.5
12	3735.0
13	3551.0
14	3367.0
15	3271.5
16	3176.0
17	3032.0
18	2888.0
19	2841.0
20	2794.0
21	3015.5
22	3237.0
23	3505.5
24	3774.0
25	4390.5
26	6220.5
27	7434.0
28	8947.0
29	10460.0
30	12117.0
31	13774.0
32	15386.5
33	16999.0
34	18705.0
35	20411.0
36	22500.5
37	24590.0
38	26451.5
39	28313.0
40	30953.5
41	33594.0
42	36109.5
43	38625.0
44	43684.0
45	48743.0
46	64485.5
47	80228.0
48	74448.0
49	68668.0
50	66802.0
51	64936.0
52	56952.0
53	48968.0
54	44166.0
55	39364.0
56	37046.0
57	34728.0
58	31673.5
59	28619.0
60	27014.0
61	25409.0
62	22718.0
63	20027.0
64	16791.5
65	13556.0
66	11244.5
67	8933.0
68	7498.5
69	6064.0
70	5010.5
71	3957.0
72	3119.0
73	2281.0
74	1783.5
75	1037.0
76	788.0
77	575.0
78	362.0
79	295.5
80	229.0
81	130.5
82	32.0
83	41.5
84	51.0
85	30.5
86	10.0
87	11.0
88	12.0
89	7.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	746267.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.21863508315965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.21216024991968	24.984453051582072
2	8.303297626413508	5.5164842767739195
3	2.855402270284372	2.8455769809660647
4	1.6298167180875511	2.16561147242333
5	1.174013989272355	1.9499571146081431
6	0.9313493446558916	1.856289240903837
7	0.8377198094956912	1.9479536057499973
8	0.756847790002144	2.0113160439661653
9	0.668985372203529	2.0000502859680687
>10	7.514527818518074	45.48526049618391
>50	0.07159667479468926	1.632671162625105
>100	0.037246824969436196	2.3431498195039606
>500	0.0024831216646290797	0.5900492270189509
>1k	0.0033108288861721062	2.063110146755218
>5k	0.0012415608323145398	2.60806707497123
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7722	1.0347502971456597	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	6191	0.8295958417027686	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC	5058	0.6777735046571803	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG	4894	0.655797455870352	No Hit
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	2733	0.3662228130146449	TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	1605	0.21507047745645994	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTT	1288	0.17259238315509062	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTATCTGT	1251	0.16763437214830618	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	1117	0.14967833228589766	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	1102	0.14766832782368777	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTC	1017	0.13627830253783163	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTA	985	0.1319902930184505	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCC	832	0.11148824750390947	No Hit
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	768	0.1029122284651472	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3400029748066042E-4	0.0	0.0	0.15356434091283683	0.0
2	1.3400029748066042E-4	0.0	0.0	0.7698317090263941	0.0
3	1.3400029748066042E-4	0.0	0.0	1.0322042914935272	0.0
4	1.3400029748066042E-4	0.0	0.0	1.5580214588076386	0.0
5	1.3400029748066042E-4	0.0	0.0	3.173663045531961	0.0
6	1.3400029748066042E-4	0.0	0.0	4.001248882772519	0.0
7	1.3400029748066042E-4	0.0	0.0	4.744548532897743	0.0
8	1.3400029748066042E-4	0.0	0.0	5.7969868693108495	0.0
9	1.3400029748066042E-4	0.0	0.0	6.229003828388499	0.0
10	1.3400029748066042E-4	0.0	0.0	7.6960390852067695	0.0
11	1.3400029748066042E-4	0.0	0.0	8.696217305602419	0.0
12	1.3400029748066042E-4	0.0	0.0	10.241240735554433	0.0
13	1.3400029748066042E-4	0.0	0.0	10.716003789528413	0.0
14	1.3400029748066042E-4	0.0	0.0	10.955998322316276	0.0
15	1.3400029748066042E-4	0.0	0.0	11.375285253133262	0.0
16	1.3400029748066042E-4	0.0	0.0	11.905256429669274	0.0
17	1.3400029748066042E-4	0.0	0.0	12.530233817119074	0.0
18	1.3400029748066042E-4	0.0	0.0	13.248877412507856	0.0
19	1.3400029748066042E-4	0.0	0.0	13.871710795197965	0.0
20	1.3400029748066042E-4	0.0	0.0	14.355049868210708	0.0
21	1.3400029748066042E-4	0.0	0.0	14.85875698644051	0.0
22	1.3400029748066042E-4	0.0	0.0	15.424104241511417	0.0
23	1.3400029748066042E-4	0.0	0.0	15.931429367773196	0.0
24	1.3400029748066042E-4	0.0	0.0	16.332224257537852	0.0
25	1.3400029748066042E-4	0.0	0.0	16.7074250904837	0.0
26	1.3400029748066042E-4	0.0	0.0	17.07244190082102	0.0
27	1.3400029748066042E-4	0.0	0.0	17.450188739419	0.0
28	1.3400029748066042E-4	0.0	0.0	17.825657572959813	0.0
29	1.3400029748066042E-4	0.0	0.0	18.198044399658567	0.0
30	1.3400029748066042E-4	0.0	0.0	18.625103347729432	0.0
31	1.3400029748066042E-4	0.0	0.0	19.025094235709204	0.0
32	1.3400029748066042E-4	0.0	0.0	19.412623096023275	0.0
33	2.6800059496132083E-4	0.0	0.0	19.818375996794714	0.0
34	2.6800059496132083E-4	0.0	0.0	20.214614876445026	0.0
35	2.6800059496132083E-4	0.0	0.0	20.601875736164136	0.0
36	2.6800059496132083E-4	0.0	0.0	20.993290605105145	0.0
37	2.6800059496132083E-4	0.0	0.0	21.374789452032584	0.0
38	2.6800059496132083E-4	0.0	0.0	21.7391362608825	0.0
39	4.020008924419812E-4	0.0	0.0	22.121841110487264	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	30	2.1644737E-6	45.000004	1
GTAACGG	65	0.0	45.000004	2
TTCTACG	20	7.0316956E-4	45.0	1
TCGGACA	25	3.889486E-5	45.0	13
ACGGCTA	40	6.8102963E-9	45.0	30
CGTAAGC	20	7.0316956E-4	45.0	43
TTTGCGC	20	7.0316956E-4	45.0	19
AGAATCG	20	7.0316956E-4	45.0	26
CGTTTTT	3795	0.0	42.806324	1
CGAACAC	70	0.0	41.785713	34
CACGACG	55	6.002665E-11	40.90909	26
CGATGAA	820	0.0	40.60976	19
CCGATGA	830	0.0	39.849396	18
CGAATAT	85	0.0	39.705883	14
CCCGTCG	35	6.2453437E-6	38.571426	40
TAATACG	65	9.094947E-12	38.076927	4
GCGACTA	30	1.1395379E-4	37.500004	33
CGACGGT	60	1.546141E-10	37.500004	28
TATTGCG	30	1.1395379E-4	37.500004	1
GTCGTTT	30	1.1395379E-4	37.500004	9
>>END_MODULE