FastQCFastQC Report
Sat 18 Jun 2016
SRR3551270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724825
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101371.3985444762528887No Hit
GCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC19680.27151381367916394TruSeq Adapter, Index 20 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTC15460.21329286379470908No Hit
CTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGCT15220.20998171972545096TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC9570.13203186976166661TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCC8960.12361604525230228No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCG7830.1080260752595454No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGC7490.10333528782809644No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7380.10181768012968648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCT502.1827873E-1145.00000430
TCCGATT207.03156E-445.020
TAGTTCG207.03156E-445.01
ACGTACG207.03156E-445.01
GACTAGT207.03156E-445.013
ATGCACG302.1643882E-644.9999961
GACACGA1000.042.75000425
CGTTTTT43250.042.6589621
ACGACCA950.042.6315828
CGGTCTA556.002665E-1140.90909231
ACGTAAG501.0804797E-940.5000041
AACACGT1000.040.50000441
ATTAGCG403.4558616E-739.3751
GATATCA403.4558616E-739.3759
TATAGCG403.4558616E-739.3751
CACGACC1100.038.8636427
AGACACG1100.038.8636424
ACGGGTA1050.038.571435
CGAACGT356.2450963E-638.57142638
TACGCGG356.2450963E-638.5714262