Basic Statistics
Measure | Value |
---|---|
Filename | SRR3551270_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 724825 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 10137 | 1.3985444762528887 | No Hit |
GCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC | 1968 | 0.27151381367916394 | TruSeq Adapter, Index 20 (95% over 21bp) |
GAATCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTC | 1546 | 0.21329286379470908 | No Hit |
CTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGCT | 1522 | 0.20998171972545096 | TruSeq Adapter, Index 23 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC | 957 | 0.13203186976166661 | TruSeq Adapter, Index 20 (95% over 21bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCC | 896 | 0.12361604525230228 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCG | 783 | 0.1080260752595454 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGC | 749 | 0.10333528782809644 | No Hit |
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 738 | 0.10181768012968648 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACGGTCT | 50 | 2.1827873E-11 | 45.000004 | 30 |
TCCGATT | 20 | 7.03156E-4 | 45.0 | 20 |
TAGTTCG | 20 | 7.03156E-4 | 45.0 | 1 |
ACGTACG | 20 | 7.03156E-4 | 45.0 | 1 |
GACTAGT | 20 | 7.03156E-4 | 45.0 | 13 |
ATGCACG | 30 | 2.1643882E-6 | 44.999996 | 1 |
GACACGA | 100 | 0.0 | 42.750004 | 25 |
CGTTTTT | 4325 | 0.0 | 42.658962 | 1 |
ACGACCA | 95 | 0.0 | 42.63158 | 28 |
CGGTCTA | 55 | 6.002665E-11 | 40.909092 | 31 |
ACGTAAG | 50 | 1.0804797E-9 | 40.500004 | 1 |
AACACGT | 100 | 0.0 | 40.500004 | 41 |
ATTAGCG | 40 | 3.4558616E-7 | 39.375 | 1 |
GATATCA | 40 | 3.4558616E-7 | 39.375 | 9 |
TATAGCG | 40 | 3.4558616E-7 | 39.375 | 1 |
CACGACC | 110 | 0.0 | 38.86364 | 27 |
AGACACG | 110 | 0.0 | 38.86364 | 24 |
ACGGGTA | 105 | 0.0 | 38.57143 | 5 |
CGAACGT | 35 | 6.2450963E-6 | 38.571426 | 38 |
TACGCGG | 35 | 6.2450963E-6 | 38.571426 | 2 |