##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551270_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	724825
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.580385610319734	31.0	31.0	34.0	30.0	34.0
2	31.734849101507262	33.0	31.0	34.0	30.0	34.0
3	31.697441451384815	33.0	31.0	34.0	30.0	34.0
4	35.41982823440141	37.0	35.0	37.0	33.0	37.0
5	34.21714758734867	37.0	35.0	37.0	32.0	37.0
6	34.71287828096437	37.0	35.0	37.0	32.0	37.0
7	35.460231090263164	37.0	35.0	37.0	33.0	37.0
8	35.33717793950264	37.0	35.0	37.0	33.0	37.0
9	37.26815024316214	39.0	37.0	39.0	34.0	39.0
10	36.690083813334255	39.0	35.0	39.0	32.0	39.0
11	36.82881661090608	39.0	37.0	39.0	33.0	39.0
12	36.85646466388439	39.0	37.0	39.0	33.0	39.0
13	36.980716724726655	39.0	37.0	39.0	33.0	39.0
14	38.01253819887559	40.0	37.0	41.0	33.0	41.0
15	38.14857241403097	40.0	37.0	41.0	33.0	41.0
16	38.17142758596903	40.0	37.0	41.0	34.0	41.0
17	38.09782913116959	40.0	37.0	41.0	33.0	41.0
18	37.961965991791125	39.0	37.0	41.0	33.0	41.0
19	37.85660952643742	39.0	37.0	41.0	34.0	41.0
20	37.931973924740454	39.0	36.0	41.0	34.0	41.0
21	37.75967026523644	39.0	36.0	41.0	33.0	41.0
22	37.88003587072742	40.0	36.0	41.0	34.0	41.0
23	37.950552202255714	40.0	36.0	41.0	34.0	41.0
24	37.8199855137447	40.0	36.0	41.0	34.0	41.0
25	37.48301728003311	39.0	35.0	41.0	33.0	41.0
26	37.63238436864071	39.0	35.0	41.0	33.0	41.0
27	37.70688235091229	39.0	35.0	41.0	33.0	41.0
28	37.587176214948435	39.0	35.0	41.0	33.0	41.0
29	37.57504363122133	40.0	35.0	41.0	33.0	41.0
30	37.24539854447625	39.0	35.0	41.0	33.0	41.0
31	37.144790811575206	39.0	35.0	41.0	33.0	41.0
32	37.125896595730005	39.0	35.0	41.0	32.0	41.0
33	37.01118476873728	39.0	35.0	41.0	32.0	41.0
34	36.80493636394992	40.0	35.0	41.0	31.0	41.0
35	36.72393750215569	40.0	35.0	41.0	31.0	41.0
36	36.50545304038906	39.0	35.0	41.0	31.0	41.0
37	36.537286931328254	39.0	35.0	41.0	31.0	41.0
38	36.334668368226815	39.0	35.0	41.0	30.0	41.0
39	36.29841409995516	39.0	35.0	41.0	30.0	41.0
40	36.18200807091367	39.0	35.0	41.0	30.0	41.0
41	36.02310074845652	39.0	35.0	41.0	29.0	41.0
42	36.1506805090884	39.0	35.0	41.0	30.0	41.0
43	36.081048528955264	39.0	35.0	41.0	30.0	41.0
44	36.13220432518194	39.0	35.0	41.0	30.0	41.0
45	36.10263304935674	39.0	35.0	41.0	30.0	41.0
46	36.0018611388956	39.0	35.0	41.0	29.0	41.0
47	35.962704100989896	39.0	35.0	41.0	29.0	41.0
48	35.89357293139723	39.0	35.0	41.0	29.0	41.0
49	35.8686041458283	39.0	35.0	41.0	29.0	41.0
50	35.76432656158383	39.0	35.0	41.0	29.0	41.0
51	34.86980995412686	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	25.0
10	45.0
11	42.0
12	33.0
13	40.0
14	53.0
15	94.0
16	105.0
17	165.0
18	278.0
19	460.0
20	669.0
21	1160.0
22	1718.0
23	2543.0
24	4003.0
25	6203.0
26	8982.0
27	10632.0
28	10555.0
29	10565.0
30	11605.0
31	13929.0
32	18093.0
33	25837.0
34	44242.0
35	55936.0
36	61303.0
37	88732.0
38	150016.0
39	196710.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.023005553064536	21.010726727141034	22.31752491980823	15.648742799986204
2	36.98396164591453	23.313213534301383	23.413927499741316	16.288897320042768
3	31.414479357086194	22.618976994446935	30.641879074259304	15.324664574207567
4	27.86686441554858	24.622357120684306	29.36019039078398	18.150588072983133
5	23.51022660642224	31.508295105715174	26.710999206705065	18.27047908115752
6	24.61835615493395	33.270651536577795	27.59300520815369	14.517987100334565
7	75.26947883971992	6.115407167247266	14.047252785155036	4.567861207877764
8	75.92674093746766	5.687441796295658	13.080812609940331	5.305004656296347
9	69.69723726416721	7.8155416824750805	15.583761597627014	6.903459455730694
10	37.43745041906667	25.021212016693685	22.571655227123788	14.969682337115856
11	28.303107646673336	24.532128444797017	28.63504983961646	18.529714068913187
12	25.402131548994582	21.77297968475149	32.54689062877246	20.27799813748146
13	23.697996068016415	23.15317490428724	34.068223364260334	19.080605663436
14	20.18473424619736	26.24343807125858	33.24968095747249	20.32214672507157
15	19.442210188666227	24.894284827372122	36.26737488359259	19.396130100369056
16	21.783740902976582	24.218397544234815	33.94750457006864	20.050356982719965
17	21.388473079708895	24.643465664125824	31.403580174524887	22.5644810816404
18	22.303176628841445	24.346566412582344	32.808746938916286	20.541510019659917
19	22.923602248818682	25.49249818921809	30.81350670851585	20.770392853447383
20	23.46345669644397	25.624668023315973	31.13372193288035	19.77815334735971
21	23.207118959748904	26.407339702686855	31.627496292208463	18.758045045355775
22	21.80981616252199	24.158107129307073	30.57193115579623	23.460145552374712
23	20.99955161590729	26.089607836374295	31.079226020073815	21.831614527644604
24	21.318801089918257	24.952643741592798	31.57231055772083	22.156244610768116
25	21.157382816541922	26.416445348877314	30.25171593143173	22.17445590314904
26	20.39788914565585	25.67405925568241	31.1926327044459	22.735418894215844
27	21.734349670610147	25.35798296140448	31.407995033283896	21.49967233470148
28	20.09257406960301	25.51567619770289	32.85524092022212	21.53650881247198
29	21.892318835581	24.312351257200014	31.242851722829652	22.552478184389336
30	22.5153657779464	24.981478287862586	31.63149725795882	20.871658676232194
31	23.4470389404339	25.74635256786121	29.2215362328838	21.585072258821096
32	23.14738040216604	26.178870761908048	28.680578070568757	21.993170765357156
33	21.820301452074638	26.061463111785603	29.16400510468044	22.954230331459318
34	21.911081985306797	25.93991653157659	29.901562446107683	22.247439037008935
35	21.440899527472148	25.88500672576139	29.49829269133929	23.175801055427172
36	22.410236953747457	27.551753871624186	28.955678956989615	21.082330217638738
37	21.285551684889455	26.927189321560373	30.215569275342325	21.571689718207843
38	20.775083640878833	28.511433794364155	27.392405063291143	23.32107750146587
39	21.771048184044425	25.423653985444762	28.762252957610457	24.043044872900353
40	21.61321698340979	26.21156830959197	30.82537164143069	21.34984306556755
41	19.570379057013763	26.55620322146725	29.643707101714206	24.22971061980478
42	21.766081467940538	26.263580864346565	29.093091435863826	22.877246231849067
43	21.561480357327632	25.053495671368953	29.846790604628705	23.538233366674717
44	21.68302693753665	24.891387576311523	29.01721105094333	24.4083744352085
45	21.474148932500945	24.864346566412582	29.468078501707307	24.193425999379162
46	22.37326251164074	25.99648190942641	29.03693995102266	22.593315627910187
47	20.782671679370882	25.089780291794572	31.217604249301555	22.90994377953299
48	20.401200289725104	25.056254958093334	30.524885317145518	24.017659435036045
49	21.43593281136826	24.02731693857138	30.746180112440936	23.790570137619426
50	20.328355120201426	23.84009933432208	31.604594212396098	24.2269513330804
51	20.780464249991375	23.117580105542718	30.050563929224296	26.05139171524161
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	427.0
1	787.5
2	1148.0
3	8363.5
4	15579.0
5	10085.5
6	4592.0
7	4375.0
8	4158.0
9	4147.5
10	4137.0
11	4077.5
12	4018.0
13	4020.5
14	4023.0
15	3783.0
16	3543.0
17	3362.0
18	3181.0
19	3110.0
20	3039.0
21	3281.5
22	3524.0
23	3726.5
24	3929.0
25	4296.0
26	5520.5
27	6378.0
28	7051.5
29	7725.0
30	9264.0
31	10803.0
32	12310.5
33	13818.0
34	15480.5
35	17143.0
36	18894.0
37	20645.0
38	22937.0
39	25229.0
40	27871.5
41	30514.0
42	33549.0
43	36584.0
44	40959.0
45	45334.0
46	55051.5
47	64769.0
48	68235.0
49	71701.0
50	70072.0
51	68443.0
52	60248.0
53	52053.0
54	45239.0
55	38425.0
56	35401.5
57	32378.0
58	30724.0
59	29070.0
60	27299.5
61	25529.0
62	22538.5
63	19548.0
64	17476.0
65	15404.0
66	13110.5
67	10817.0
68	9528.0
69	8239.0
70	6954.5
71	5670.0
72	4519.0
73	3368.0
74	2901.0
75	1981.0
76	1528.0
77	1103.0
78	678.0
79	527.5
80	377.0
81	225.5
82	74.0
83	88.5
84	103.0
85	71.5
86	40.0
87	21.0
88	2.0
89	2.0
90	2.0
91	6.5
92	11.0
93	17.0
94	23.0
95	14.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	724825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.95323050227329
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.17176659512405	25.52324318467335
2	9.352559462509689	6.350992144336173
3	3.306333851758668	3.3678214615869346
4	1.816891263262021	2.4675731143640767
5	1.1494624045898094	1.951398098836756
6	0.8691931272835993	1.770714876099109
7	0.6757971220628196	1.6061846820720285
8	0.6055879641120406	1.6449334187918816
9	0.5107830270277497	1.5608460467989866
>10	6.369163696132091	45.12009534619089
>50	0.12163950234665756	2.638991392444581
>100	0.04540652650165654	2.9316311787005533
>500	0.003749162738668889	0.9195027882825444
>1k	0.0012497209128896295	0.7122928844471456
>5k	0.0	0.0
>10k+	4.165736376298765E-4	1.4337793823750427
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10137	1.3985444762528887	No Hit
GCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC	1968	0.27151381367916394	TruSeq Adapter, Index 20 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTC	1546	0.21329286379470908	No Hit
CTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGCT	1522	0.20998171972545096	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC	957	0.13203186976166661	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCC	896	0.12361604525230228	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCG	783	0.1080260752595454	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGC	749	0.10333528782809644	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	738	0.10181768012968648	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7592867243817474E-4	0.0	0.0	0.21977718759700618	0.0
2	2.7592867243817474E-4	0.0	0.0	0.8029524367950884	0.0
3	2.7592867243817474E-4	0.0	0.0	1.1351705584106508	0.0
4	2.7592867243817474E-4	0.0	0.0	1.6261856310143827	0.0
5	2.7592867243817474E-4	0.0	0.0	2.833097644258959	0.0
6	2.7592867243817474E-4	0.0	0.0	3.8162315041561756	0.0
7	2.7592867243817474E-4	0.0	0.0	4.684165143310454	0.0
8	2.7592867243817474E-4	0.0	0.0	6.174042010140378	0.0
9	2.7592867243817474E-4	0.0	0.0	6.860276618494119	0.0
10	2.7592867243817474E-4	0.0	0.0	8.158107129307075	0.0
11	2.7592867243817474E-4	0.0	0.0	9.564239644052012	0.0
12	2.7592867243817474E-4	0.0	0.0	10.815990066567792	0.0
13	2.7592867243817474E-4	0.0	0.0	11.364260338702445	0.0
14	2.7592867243817474E-4	0.0	0.0	11.620736039733728	0.0
15	2.7592867243817474E-4	0.0	0.0	11.95626530541855	0.0
16	2.7592867243817474E-4	0.0	0.0	12.564825992480944	0.0
17	2.7592867243817474E-4	0.0	0.0	13.282516469492636	0.0
18	2.7592867243817474E-4	0.0	0.0	14.09954126858207	0.0
19	2.7592867243817474E-4	0.0	0.0	14.629324319663366	0.0
20	4.138930086572621E-4	0.0	0.0	15.150001724554203	0.0
21	4.138930086572621E-4	0.0	0.0	15.735108474459352	0.0
22	4.138930086572621E-4	0.0	0.0	16.350015520987824	0.0
23	4.138930086572621E-4	0.0	0.0	16.969475390611528	0.0
24	4.138930086572621E-4	0.0	0.0	17.455247818438934	0.0
25	4.138930086572621E-4	0.0	0.0	17.907908805573758	0.0
26	4.138930086572621E-4	0.0	0.0	18.337943641568653	0.0
27	4.138930086572621E-4	0.0	0.0	18.774324837029628	0.0
28	4.138930086572621E-4	0.0	0.0	19.21249956886145	0.0
29	4.138930086572621E-4	0.0	0.0	19.655778981133377	0.0
30	4.138930086572621E-4	0.0	0.0	20.181974959472978	0.0
31	4.138930086572621E-4	0.0	0.0	20.6830614286207	0.0
32	4.138930086572621E-4	0.0	0.0	21.163315282999346	0.0
33	4.138930086572621E-4	0.0	0.0	21.659159107370744	0.0
34	4.138930086572621E-4	0.0	0.0	22.135411995999036	0.0
35	4.138930086572621E-4	0.0	0.0	22.637602179836513	0.0
36	5.518573448763495E-4	0.0	0.0	23.073983375297484	0.0
37	5.518573448763495E-4	0.0	0.0	23.53257682888973	0.0
38	5.518573448763495E-4	0.0	0.0	23.977511813196287	0.0
39	5.518573448763495E-4	0.0	0.0	24.452936915807264	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGTCT	50	2.1827873E-11	45.000004	30
TCCGATT	20	7.03156E-4	45.0	20
TAGTTCG	20	7.03156E-4	45.0	1
ACGTACG	20	7.03156E-4	45.0	1
GACTAGT	20	7.03156E-4	45.0	13
ATGCACG	30	2.1643882E-6	44.999996	1
GACACGA	100	0.0	42.750004	25
CGTTTTT	4325	0.0	42.658962	1
ACGACCA	95	0.0	42.63158	28
CGGTCTA	55	6.002665E-11	40.909092	31
ACGTAAG	50	1.0804797E-9	40.500004	1
AACACGT	100	0.0	40.500004	41
ATTAGCG	40	3.4558616E-7	39.375	1
GATATCA	40	3.4558616E-7	39.375	9
TATAGCG	40	3.4558616E-7	39.375	1
CACGACC	110	0.0	38.86364	27
AGACACG	110	0.0	38.86364	24
ACGGGTA	105	0.0	38.57143	5
CGAACGT	35	6.2450963E-6	38.571426	38
TACGCGG	35	6.2450963E-6	38.571426	2
>>END_MODULE