SRR3551268_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551268_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	784978
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13219	1.683996239385053	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTC	5809	0.7400207394347358	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCG	4075	0.5191228289200461	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGC	3770	0.48026823681682795	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	3575	0.45542677629181966	No Hit
CTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGCT	2596	0.3307099052457521	TruSeq Adapter, Index 15 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCT	2017	0.2569498763022658	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	1939	0.24701329209226244	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCC	1684	0.21452830525186692	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTG	1497	0.19070598156891022	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTT	1301	0.16573712893864542	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTC	1194	0.15210617367620494	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGAGTGT	1161	0.14790223420274198	No Hit
TCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	1013	0.12904820262478695	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCT	978	0.12458947894081107	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCT	968	0.12331555788824655	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTT	900	0.11465289473080774	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTC	843	0.10739154473118992	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	30	2.1646192E-6	45.000004	1
TATCGCG	30	2.1646192E-6	45.000004	1
GTCGAAT	35	1.211265E-7	45.0	43
CCGCTCG	20	7.0319185E-4	45.0	19
CGTTATG	20	7.0319185E-4	45.0	1
TCGTCCC	25	3.889672E-5	45.0	38
CGAATAT	55	1.8189894E-12	45.0	14
CGGTCTA	20	7.0319185E-4	45.0	31
CGTTTTT	6740	0.0	43.66469	1
TGTGACG	45	1.9270374E-8	40.0	1
ACGTAAG	45	1.9270374E-8	40.0	1
CTAGCGG	85	0.0	39.705883	2
CGTTATT	285	0.0	39.473686	1
TTCACGG	40	3.4563527E-7	39.375	2
GTTACGG	70	0.0	38.571426	2
CCGACCG	35	6.2457584E-6	38.571426	15
CGATGAA	415	0.0	38.493973	19
TCACGGG	170	0.0	38.382355	3
TACGAAT	65	9.094947E-12	38.07692	12
TACGGGA	225	0.0	38.0	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content