##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551268_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	784978
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60632909457335	31.0	31.0	34.0	30.0	34.0
2	31.77299745980142	33.0	31.0	34.0	30.0	34.0
3	31.73344985464561	33.0	31.0	34.0	30.0	34.0
4	35.45074511642365	37.0	35.0	37.0	33.0	37.0
5	34.265632922196545	37.0	35.0	37.0	32.0	37.0
6	34.75450140003924	37.0	35.0	37.0	32.0	37.0
7	35.48876529023743	37.0	35.0	37.0	33.0	37.0
8	35.359746387796854	37.0	35.0	37.0	33.0	37.0
9	37.26118311595994	39.0	37.0	39.0	34.0	39.0
10	36.68352743643771	39.0	35.0	39.0	32.0	39.0
11	36.89140077811098	39.0	37.0	39.0	33.0	39.0
12	36.9167709158728	39.0	37.0	39.0	33.0	39.0
13	37.0526779094446	39.0	37.0	39.0	33.0	39.0
14	38.112459202678295	40.0	37.0	41.0	33.0	41.0
15	38.20623635312072	40.0	37.0	41.0	33.0	41.0
16	38.261620070880966	40.0	37.0	41.0	34.0	41.0
17	38.18818361788483	40.0	37.0	41.0	34.0	41.0
18	38.01814445755168	39.0	37.0	41.0	34.0	41.0
19	37.83289977553511	39.0	37.0	41.0	34.0	41.0
20	37.82903724690374	39.0	36.0	41.0	34.0	41.0
21	37.711407198673086	39.0	35.0	41.0	33.0	41.0
22	37.838738155719014	39.0	35.0	41.0	34.0	41.0
23	37.914268934925566	40.0	35.0	41.0	34.0	41.0
24	37.78441561419556	40.0	35.0	41.0	34.0	41.0
25	37.50427145728925	39.0	35.0	41.0	33.0	41.0
26	37.62218686383567	39.0	35.0	41.0	33.0	41.0
27	37.68535169138498	39.0	35.0	41.0	33.0	41.0
28	37.55347156226034	39.0	35.0	41.0	33.0	41.0
29	37.5355411234455	39.0	35.0	41.0	33.0	41.0
30	37.20280313588406	39.0	35.0	41.0	33.0	41.0
31	36.9999732476579	39.0	35.0	41.0	32.0	41.0
32	36.8607362244547	39.0	35.0	41.0	32.0	41.0
33	36.64007908501895	39.0	35.0	41.0	31.0	41.0
34	36.30815640693115	39.0	35.0	41.0	30.0	41.0
35	36.184444659595556	39.0	35.0	41.0	30.0	41.0
36	35.9236997724777	39.0	35.0	41.0	27.0	41.0
37	35.91555049950445	39.0	35.0	41.0	28.0	41.0
38	35.708523041410075	39.0	35.0	41.0	26.0	41.0
39	35.69695329041069	39.0	35.0	41.0	26.0	41.0
40	35.603981258073475	39.0	35.0	41.0	26.0	41.0
41	35.460123723212625	39.0	35.0	40.0	25.0	41.0
42	35.55407794868137	39.0	35.0	41.0	25.0	41.0
43	35.5357156506297	39.0	35.0	41.0	25.0	41.0
44	35.60143851165255	39.0	35.0	41.0	25.0	41.0
45	35.607362244546984	39.0	35.0	41.0	25.0	41.0
46	35.533141056182465	39.0	35.0	41.0	25.0	41.0
47	35.46941825121213	39.0	35.0	41.0	25.0	41.0
48	35.428457867609026	39.0	35.0	41.0	25.0	41.0
49	35.413132597346674	39.0	35.0	41.0	25.0	41.0
50	35.31648785061492	39.0	35.0	40.0	24.0	41.0
51	34.36345349805982	37.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	28.0
10	30.0
11	28.0
12	31.0
13	37.0
14	50.0
15	65.0
16	93.0
17	172.0
18	274.0
19	560.0
20	961.0
21	1639.0
22	2390.0
23	3668.0
24	5746.0
25	9594.0
26	12871.0
27	14359.0
28	12988.0
29	12344.0
30	12915.0
31	15253.0
32	19237.0
33	26491.0
34	45051.0
35	57038.0
36	63447.0
37	94959.0
38	166191.0
39	206406.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.62406844523031	19.018622177946387	22.25170641724991	18.10560295957339
2	38.06017493483893	24.13124953820362	22.87414424353294	14.934431283424503
3	29.46655829844913	23.419509846135817	32.2138964404098	14.900035415005261
4	25.62721503023015	22.813505601430865	34.46300405871247	17.096275309626513
5	23.674548840859234	29.195213114252883	30.013452606315084	17.1167854385728
6	22.83235963300882	32.64397218775558	30.834367332587664	13.689300846647932
7	71.1687461304648	5.2504656181447125	18.72905992269847	4.8517283286920145
8	71.74978152253948	6.353681249665595	16.360968078086266	5.535569149708654
9	66.19051744125312	6.41712251808331	18.55809971744431	8.83426032321925
10	35.12353212446718	22.67260993301723	26.736672874908596	15.467185067606989
11	27.39427092224241	23.625630272440755	30.3158814641939	18.664217341122935
12	22.92803110405642	20.504523693657656	36.06559674283865	20.501848459447274
13	22.495789690921274	22.09985502778422	37.994058432210835	17.410296849083668
14	19.323598877930337	25.92913431968794	35.19716476130541	19.55010204107631
15	18.02789377536695	24.506928856604898	39.31982807161475	18.1453492964134
16	19.66004142791263	24.15774709609696	35.517810690235905	20.664400785754506
17	20.04998866210263	24.79993069869474	33.81152083243097	21.338559806771652
18	20.618030059441157	23.55467286981291	35.12123906657257	20.706058004173368
19	20.622616175230387	26.35436916703398	32.636328661440196	20.386685996295437
20	21.68965244885844	25.64288425917669	34.058534124523234	18.608929167441634
21	21.230021733093157	26.831834777535168	34.0194247482095	17.918718741162174
22	19.002443380578818	23.943600967160865	33.7637487929598	23.290206859300515
23	19.4038559042419	25.71689907233069	33.43291149560879	21.446333527818613
24	20.1697372410437	24.56616618554915	33.350743587718384	21.91335298568877
25	19.152001712149893	27.73746525380329	31.619739661493696	21.490793372553117
26	18.50969071744686	26.821388624904134	33.54578090086601	21.123139756782994
27	21.706722990962803	25.4199480749779	32.23185872725096	20.641470206808343
28	18.67020477006999	25.4702679565542	34.923781303425066	20.93574596995075
29	20.900840533110483	25.202107574989363	32.68078850617469	21.21626338572546
30	21.998705696210592	26.529278527551092	31.930576398319445	19.54143937791887
31	22.2356550119876	25.265548843407075	31.419224487820042	21.079571656785287
32	23.461167064554676	26.89196385121621	30.536397198392823	19.11047188583629
33	23.52970401718265	26.171816280201483	29.357128480033833	20.941351222582032
34	22.467890819870114	26.34723520913962	30.719714437856858	20.465159533133413
35	23.823215427693516	25.31319349077299	30.219827816830534	20.64376326470296
36	24.10436980399451	27.275668872248648	29.791535558958337	18.828425764798503
37	24.62718700396699	25.7566454091707	31.23654420888229	18.37962337798002
38	22.046859911997537	26.905467414373398	30.43805049313484	20.60962218049423
39	25.393705301295068	25.459184843396887	29.042979548471422	20.104130306836623
40	23.070073301417366	25.00681547763122	31.953124800949833	19.96998642000158
41	22.676686480385438	25.29816122235273	29.9832606773693	22.04189161989253
42	22.88701084616384	24.91394663289927	31.804330822010296	20.394711698926592
43	23.979143364527413	24.205773919778643	30.739969782592635	21.07511293310131
44	22.681782164595695	24.686933901332267	30.048230651050094	22.583053283021943
45	22.516299819867562	23.966531546107024	29.694080598437157	23.823088035588256
46	23.935957440845478	25.43892949866111	29.836887148429636	20.788225912063776
47	21.091036946258367	24.844263151323986	33.11787081930959	20.946829083108064
48	21.779081706748467	24.08385967504822	31.52941865886687	22.60763995933644
49	22.356804904086484	22.53757430144539	33.28615579035336	21.819465004114765
50	21.69831511201588	22.297694967247487	32.43950785881897	23.56448206191766
51	20.928994188372158	22.45425986460767	30.534613708919228	26.08213223810094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	310.0
1	599.0
2	888.0
3	10802.5
4	20717.0
5	13662.0
6	6607.0
7	6427.0
8	6247.0
9	6269.0
10	6291.0
11	6175.5
12	6060.0
13	5933.0
14	5806.0
15	5647.0
16	5488.0
17	5076.5
18	4665.0
19	4435.0
20	4205.0
21	4124.0
22	4043.0
23	4079.5
24	4116.0
25	4560.0
26	5655.0
27	6306.0
28	7069.0
29	7832.0
30	9497.5
31	11163.0
32	12410.0
33	13657.0
34	15405.5
35	17154.0
36	18962.0
37	20770.0
38	22323.0
39	23876.0
40	28002.0
41	32128.0
42	36562.5
43	40997.0
44	46298.5
45	51600.0
46	69346.5
47	87093.0
48	86466.5
49	85840.0
50	83410.0
51	80980.0
52	68398.0
53	55816.0
54	48380.5
55	40945.0
56	36683.5
57	32422.0
58	28753.0
59	25084.0
60	23564.5
61	22045.0
62	19124.0
63	16203.0
64	13972.0
65	11741.0
66	9623.5
67	7506.0
68	6050.5
69	4595.0
70	4053.5
71	3512.0
72	2748.5
73	1985.0
74	1778.5
75	1262.0
76	952.0
77	670.5
78	389.0
79	325.5
80	262.0
81	159.5
82	57.0
83	33.5
84	10.0
85	15.0
86	20.0
87	16.5
88	13.0
89	8.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	784978.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.02140842082757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.38465663620812	29.416154752653267
2	9.770327714719299	7.625038963227852
3	3.6662681078477295	4.291888356497429
4	1.9961401172638904	3.115687951238116
5	1.352976284129138	2.639752008334837
6	0.9875011821170461	2.3120212166063565
7	0.796187576362321	2.174785241777608
8	0.6657543596823339	2.078293822168871
9	0.5377466920069832	1.8885269966178115
>10	4.728777217664859	33.58867485929313
>50	0.0712072273513721	1.8903653829089138
>100	0.03443353636718923	2.133003961212665
>500	0.0033430617832218954	0.9950800694837656
>1k	0.0040116741398662755	3.3685051853971935
>5k	3.3430617832218957E-4	0.7577897382841104
>10k+	3.3430617832218957E-4	1.724431494298099
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13219	1.683996239385053	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTC	5809	0.7400207394347358	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCG	4075	0.5191228289200461	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGC	3770	0.48026823681682795	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	3575	0.45542677629181966	No Hit
CTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGCT	2596	0.3307099052457521	TruSeq Adapter, Index 15 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCT	2017	0.2569498763022658	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	1939	0.24701329209226244	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCC	1684	0.21452830525186692	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTG	1497	0.19070598156891022	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTT	1301	0.16573712893864542	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTC	1194	0.15210617367620494	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGAGTGT	1161	0.14790223420274198	No Hit
TCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	1013	0.12904820262478695	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCT	978	0.12458947894081107	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCT	968	0.12331555788824655	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTT	900	0.11465289473080774	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTC	843	0.10739154473118992	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.34166562629780706	0.0
2	1.2739210525645304E-4	0.0	0.0	1.2895902815110742	0.0
3	1.2739210525645304E-4	0.0	0.0	1.7935534499056025	0.0
4	1.2739210525645304E-4	0.0	0.0	2.5610908840757323	0.0
5	1.2739210525645304E-4	0.0	0.0	4.622932107651424	0.0
6	1.2739210525645304E-4	0.0	0.0	5.921185052319937	0.0
7	1.2739210525645304E-4	0.0	0.0	7.069879665417375	0.0
8	1.2739210525645304E-4	0.0	0.0	8.69846033901587	0.0
9	1.2739210525645304E-4	0.0	0.0	9.428671886345859	0.0
10	1.2739210525645304E-4	0.0	0.0	11.323119883614572	0.0
11	1.2739210525645304E-4	0.0	0.0	13.085334875627087	0.0
12	1.2739210525645304E-4	0.0	0.0	14.909207646583726	0.0
13	1.2739210525645304E-4	0.0	0.0	15.587443214969081	0.0
14	1.2739210525645304E-4	0.0	0.0	15.904522164952393	0.0
15	1.2739210525645304E-4	0.0	0.0	16.427084580714364	0.0
16	1.2739210525645304E-4	0.0	0.0	17.218316946462195	0.0
17	1.2739210525645304E-4	0.0	0.0	18.20904534904163	0.0
18	1.2739210525645304E-4	0.0	0.0	19.277737720038015	0.0
19	1.2739210525645304E-4	0.0	0.0	20.07737796473277	0.0
20	1.2739210525645304E-4	0.0	0.0	20.730772072593115	0.0
21	1.2739210525645304E-4	0.0	0.0	21.451047035713103	0.0
22	1.2739210525645304E-4	0.0	0.0	22.235018051461314	0.0
23	1.2739210525645304E-4	0.0	0.0	23.015549480367604	0.0
24	1.2739210525645304E-4	0.0	0.0	23.640025580334736	0.0
25	1.2739210525645304E-4	0.0	0.0	24.204245214515566	0.0
26	1.2739210525645304E-4	0.0	0.0	24.72375021975138	0.0
27	2.547842105129061E-4	0.0	0.0	25.273064977617206	0.0
28	2.547842105129061E-4	0.0	0.0	25.80594615390495	0.0
29	2.547842105129061E-4	0.0	0.0	26.359719635454752	0.0
30	3.8217631576935914E-4	0.0	0.0	26.984450519632396	0.0
31	5.095684210258122E-4	0.0	0.0	27.554530190655026	0.0
32	5.095684210258122E-4	0.0	0.0	28.10702975115226	0.0
33	5.095684210258122E-4	0.0	0.0	28.648318806386932	0.0
34	5.095684210258122E-4	0.0	0.0	29.171390790569927	0.0
35	5.095684210258122E-4	0.0	0.0	29.701851516857797	0.0
36	6.369605262822653E-4	0.0	0.0	30.230910929987846	0.0
37	6.369605262822653E-4	0.0	0.0	30.766212556275462	0.0
38	6.369605262822653E-4	0.0	0.0	31.271194861512043	0.0
39	6.369605262822653E-4	0.0	0.0	31.793120316747732	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	30	2.1646192E-6	45.000004	1
TATCGCG	30	2.1646192E-6	45.000004	1
GTCGAAT	35	1.211265E-7	45.0	43
CCGCTCG	20	7.0319185E-4	45.0	19
CGTTATG	20	7.0319185E-4	45.0	1
TCGTCCC	25	3.889672E-5	45.0	38
CGAATAT	55	1.8189894E-12	45.0	14
CGGTCTA	20	7.0319185E-4	45.0	31
CGTTTTT	6740	0.0	43.66469	1
TGTGACG	45	1.9270374E-8	40.0	1
ACGTAAG	45	1.9270374E-8	40.0	1
CTAGCGG	85	0.0	39.705883	2
CGTTATT	285	0.0	39.473686	1
TTCACGG	40	3.4563527E-7	39.375	2
GTTACGG	70	0.0	38.571426	2
CCGACCG	35	6.2457584E-6	38.571426	15
CGATGAA	415	0.0	38.493973	19
TCACGGG	170	0.0	38.382355	3
TACGAAT	65	9.094947E-12	38.07692	12
TACGGGA	225	0.0	38.0	4
>>END_MODULE