FastQCFastQC Report
Sat 18 Jun 2016
SRR3551266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726434
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103281.4217396212181699No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG102671.4133424371656613No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC96701.3311601604550447No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC79321.0919092443360305No Hit
GCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC38660.5321887466720996No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAAGCCAT30100.41435285242706155No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT21500.2959663231621868No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTA17860.2458585363570538No Hit
GAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT15820.21777615034538583No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCACCCATCGAATGC14760.2031843223197152No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTC14600.2009817822403687No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT13430.18487570791014737No Hit
CCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC8520.11728525922520146No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA8120.1117789090268352No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCC7510.10338172497432664No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7280.10021557361026603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG351.2111013E-745.0000041
TGCGTAG351.2111013E-745.0000041
TGTGCGA207.0315704E-445.01
TGTTGCG253.8893828E-545.01
ACTAGCG207.0315704E-445.01
CGTGTTC207.0315704E-445.023
ATAACGG207.0315704E-445.02
ACGTAAG207.0315704E-445.01
TGTCGGA207.0315704E-445.029
CGAACCC1200.044.99999634
CTAGTCG302.1643955E-644.99999629
CGAATGC3700.043.17567445
CGTTTTT43250.043.075141
GATGAAT23350.043.07280320
ATCGAAT4300.042.38372443
CGATGAA22600.042.1128319
CCGATGA21650.041.88221718
CCCATCG4800.041.71874640
TGAATGA24250.041.5670122
GCGAACC1300.041.5384633