##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551266_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	726434
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60484916730219	31.0	31.0	34.0	30.0	34.0
2	31.77664178714102	33.0	31.0	34.0	30.0	34.0
3	31.76228397899878	33.0	31.0	34.0	30.0	34.0
4	35.48811454309683	37.0	35.0	37.0	33.0	37.0
5	34.26622101939061	37.0	35.0	37.0	32.0	37.0
6	34.741536051451334	37.0	35.0	37.0	32.0	37.0
7	35.43397748453404	37.0	35.0	37.0	33.0	37.0
8	35.070526709928224	37.0	35.0	37.0	32.0	37.0
9	36.85884195948978	39.0	37.0	39.0	32.0	39.0
10	36.61691771034946	39.0	35.0	39.0	32.0	39.0
11	36.80526241888458	39.0	37.0	39.0	33.0	39.0
12	36.75958862057668	39.0	35.0	39.0	33.0	39.0
13	36.761368548278305	39.0	35.0	39.0	32.0	39.0
14	37.828456542507645	40.0	37.0	41.0	33.0	41.0
15	37.90723864797077	40.0	37.0	41.0	33.0	41.0
16	38.059537411519834	40.0	37.0	41.0	33.0	41.0
17	37.85473284565425	40.0	37.0	41.0	33.0	41.0
18	37.811000035791274	39.0	37.0	41.0	33.0	41.0
19	37.67260618308064	39.0	37.0	41.0	33.0	41.0
20	37.44201262606101	39.0	35.0	41.0	32.0	41.0
21	37.522994518428376	39.0	35.0	41.0	33.0	41.0
22	37.58891791959077	39.0	35.0	41.0	33.0	41.0
23	37.484668944460196	39.0	35.0	41.0	33.0	41.0
24	37.4684967939276	39.0	35.0	41.0	33.0	41.0
25	37.32666422551808	39.0	35.0	41.0	33.0	41.0
26	37.29410655338269	39.0	35.0	41.0	33.0	41.0
27	37.21948449549443	39.0	35.0	41.0	32.0	41.0
28	37.1366882607367	39.0	35.0	41.0	32.0	41.0
29	37.16864298752537	39.0	35.0	41.0	32.0	41.0
30	36.899237921132546	39.0	35.0	41.0	31.0	41.0
31	36.73458290773835	39.0	35.0	41.0	31.0	41.0
32	36.49085945867071	39.0	35.0	41.0	30.0	41.0
33	36.36912093872258	39.0	35.0	41.0	30.0	41.0
34	36.140121470085376	39.0	35.0	41.0	29.0	41.0
35	35.995607309129255	39.0	35.0	41.0	27.0	41.0
36	35.8532241056999	39.0	35.0	41.0	27.0	41.0
37	35.70094186120143	39.0	35.0	41.0	26.0	41.0
38	35.531069580994284	39.0	35.0	41.0	24.0	41.0
39	35.51632770492571	39.0	35.0	41.0	24.0	41.0
40	35.473698918277506	39.0	35.0	41.0	25.0	41.0
41	35.41441617545434	39.0	35.0	40.0	25.0	41.0
42	35.451625336919804	39.0	35.0	41.0	25.0	41.0
43	35.36596992982157	39.0	35.0	41.0	24.0	41.0
44	35.47491719825889	39.0	35.0	41.0	25.0	41.0
45	35.442460017014625	39.0	35.0	41.0	24.0	41.0
46	35.37142947604325	39.0	35.0	41.0	24.0	41.0
47	35.15776794588359	38.0	35.0	41.0	23.0	41.0
48	35.200618363127276	38.0	35.0	41.0	23.0	41.0
49	35.220047519802215	38.0	35.0	41.0	24.0	41.0
50	35.088313597656494	38.0	35.0	40.0	24.0	41.0
51	34.256295823158055	37.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	21.0
10	30.0
11	35.0
12	16.0
13	39.0
14	43.0
15	77.0
16	100.0
17	168.0
18	240.0
19	553.0
20	1153.0
21	2803.0
22	5054.0
23	6850.0
24	7989.0
25	9500.0
26	11274.0
27	11534.0
28	11318.0
29	10838.0
30	11512.0
31	14177.0
32	18335.0
33	25747.0
34	44164.0
35	56599.0
36	57651.0
37	83387.0
38	139908.0
39	195257.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.953245580465676	18.32953854032163	20.546670447693803	15.1705454315189
2	33.23481555103423	30.154287932558223	21.042379624301727	15.568516892105821
3	29.127078303052993	29.842628511330695	27.16103596472632	13.869257220889992
4	25.95308038995972	22.114878984188515	35.48925298100034	16.442787644851425
5	28.97703026014751	28.446631077289886	24.626738285928244	17.949600376634354
6	22.684235594699587	38.499849951957096	26.02989397522693	12.786020478116388
7	68.51881932839046	5.895098522370924	21.228081284741627	4.358000864496981
8	67.9892460980626	13.290952791306573	13.715079415335735	5.004721695295099
9	62.8291076684186	7.515479726995157	15.185550235809448	14.469862368776793
10	40.58111266818458	22.418141221363538	21.81175440576845	15.188991704683428
11	32.344989358978246	22.6882276985934	27.2200915706038	17.746691371824557
12	25.785549685174423	20.290212187204894	30.550469829330673	23.373768298290003
13	26.265152787452127	22.385791413948137	34.2672562132279	17.08179958537183
14	19.877373581082384	30.325260106217495	29.664360423658586	20.133005889041534
15	18.04775106892023	23.734434236282993	35.96747949572845	22.250335199068324
16	19.453935250828017	23.104370114834932	31.62998978572038	25.811704848616667
17	20.175267126814	26.501375210962042	32.28084037916727	21.042517283056686
18	20.8675530055036	22.93560048125501	31.603421646013263	24.59342486722813
19	21.36023368950242	24.62962911978239	29.534135241467226	24.47600194924797
20	25.16209318396441	23.477975975794084	33.60883438825826	17.75109645198325
21	22.24510416637988	29.10023484583596	30.38982206229334	18.26483892549082
22	20.577506008804654	22.02457484093531	34.11775880534226	23.28016034491778
23	23.283877131301672	26.00071031917559	30.622052381909437	20.0933601676133
24	21.53258245071128	26.138782050399623	30.002587984593234	22.326047514295862
25	19.129886541654162	30.12042387883827	29.60103739637737	21.148652183130196
26	19.132639716753346	24.159662130351826	34.896632040901174	21.811066111993657
27	24.608429671518678	25.13373548044282	29.98496766395846	20.27286718408004
28	18.035774757238784	28.346415503679616	33.18539605800389	20.4324136810777
29	21.414333580201365	23.455812916245662	34.590341311117044	20.53951219243593
30	22.52372548641721	28.291214342940997	30.334620901554715	18.850439269087076
31	21.923395656040327	25.486141893138264	27.374407034913013	25.216055415908396
32	24.888565237860565	28.18260158527822	27.45328550150461	19.475547675356605
33	25.01163216479405	24.811338676328475	26.917517627203573	23.259511531673905
34	20.61632577770314	25.46865923125845	29.015024076516244	24.89999091452217
35	24.617239831836066	23.96983070726315	27.540285834638794	23.872643626261986
36	21.087256378418413	30.914026601177806	27.89916220881732	20.09955481158646
37	22.23477975975794	27.718140946046027	28.46700457302384	21.580074721172192
38	21.667901006836136	28.759667086067008	25.108543928285293	24.463887978811563
39	22.008881742869963	27.524179760308577	27.335174289749652	23.131764207071807
40	22.736683580339026	23.12584488060856	28.406021744576933	25.73144979447548
41	19.13787074944179	26.88076273962948	26.795276652799842	27.186089858128888
42	20.19619125756779	25.73929634350815	29.375414696999314	24.689097701924744
43	22.20380653989213	27.467189035755485	27.605673743244395	22.723330681107985
44	21.929039664993656	24.677672025263135	29.837948113662083	23.555340196081133
45	21.086568084643613	23.890952240671552	28.162778724564102	26.859700950120725
46	24.153742803888584	27.254506259343586	27.351142705324914	21.240608231442913
47	19.652576834234083	26.66807996321758	32.542529672344635	21.136813530203707
48	21.029852677600445	26.196461068727512	28.57933411707051	24.194352136601534
49	20.708281826015853	23.38271611736235	32.810826585760026	23.09817547086177
50	21.914585495722942	24.11946577390375	30.051456842603734	23.91449188776957
51	20.309071436634298	23.214359460047298	29.10463992599465	27.37192917732375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	280.0
1	707.0
2	1134.0
3	8870.0
4	16606.0
5	10855.0
6	5104.0
7	4749.0
8	4394.0
9	4351.5
10	4309.0
11	4141.0
12	3973.0
13	3802.5
14	3632.0
15	3507.5
16	3383.0
17	3209.5
18	3036.0
19	3050.5
20	3065.0
21	3547.5
22	4030.0
23	4244.0
24	4458.0
25	4534.0
26	5420.5
27	6231.0
28	6829.5
29	7428.0
30	8767.5
31	10107.0
32	11620.5
33	13134.0
34	14331.0
35	15528.0
36	17036.0
37	18544.0
38	19785.0
39	21026.0
40	24025.0
41	27024.0
42	30819.5
43	34615.0
44	38904.5
45	43194.0
46	64733.5
47	86273.0
48	76320.0
49	66367.0
50	64662.0
51	62957.0
52	57425.5
53	51894.0
54	48116.0
55	44338.0
56	39357.5
57	34377.0
58	31759.5
59	29142.0
60	27423.5
61	25705.0
62	23258.5
63	20812.0
64	18643.0
65	16474.0
66	13799.0
67	11124.0
68	9266.5
69	7409.0
70	5667.0
71	3925.0
72	3377.5
73	2830.0
74	2263.5
75	1398.0
76	1099.0
77	988.0
78	877.0
79	520.5
80	164.0
81	121.5
82	79.0
83	56.0
84	33.0
85	18.5
86	4.0
87	2.5
88	1.0
89	3.0
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	726434.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.782882589018243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.03983674712202	22.646855299276503
2	9.059325221837547	5.396256388354387
3	3.1610333737350484	2.8243405748975756
4	1.5684410659222683	1.8685078445663017
5	1.013846604485117	1.509763719232753
6	0.7556466526422463	1.3503201320657203
7	0.5756616972478209	1.2001405318088771
8	0.5037175960861873	1.2001729617805945
9	0.4250081591944115	1.1392171294195739
>10	6.595186026911032	44.91859984330133
>50	0.23959131527341027	4.405192151227726
>100	0.051474696639669464	2.803696864864739
>500	0.005615421451600305	1.0897303666738278
>1k	0.0037436143010668695	2.3237081984336525
>5k	9.359035752667174E-4	2.45318249318234
>10k+	9.359035752667174E-4	2.870315500914117
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10328	1.4217396212181699	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCG	10267	1.4133424371656613	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGC	9670	1.3311601604550447	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC	7932	1.0919092443360305	No Hit
GCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	3866	0.5321887466720996	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAAGCCAT	3010	0.41435285242706155	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT	2150	0.2959663231621868	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTA	1786	0.2458585363570538	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT	1582	0.21777615034538583	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCACCCATCGAATGC	1476	0.2031843223197152	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTC	1460	0.2009817822403687	No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT	1343	0.18487570791014737	No Hit
CCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	852	0.11728525922520146	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	812	0.1117789090268352	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCC	751	0.10338172497432664	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	728	0.10021557361026603	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19230928067794184	0.0
2	0.0	0.0	0.0	1.0104152614002098	0.0
3	0.0	0.0	0.0	1.2308069280898195	0.0
4	0.0	0.0	0.0	1.7035270926195636	0.0
5	0.0	0.0	0.0	3.5524218304759962	0.0
6	0.0	0.0	0.0	4.0972751826043385	0.0
7	0.0	0.0	0.0	4.856738533714005	0.0
8	0.0	0.0	0.0	5.743260915650975	0.0
9	0.0	0.0	0.0	6.038538945038366	0.0
10	0.0	0.0	0.0	8.198404810347533	0.0
11	0.0	0.0	0.0	9.214739398210988	0.0
12	0.0	0.0	0.0	11.44370995850965	0.0
13	0.0	0.0	0.0	11.897570873609991	0.0
14	0.0	0.0	0.0	12.10502261733344	0.0
15	0.0	0.0	0.0	12.643406007978701	0.0
16	0.0	0.0	0.0	13.260117230195723	0.0
17	0.0	0.0	0.0	13.912895046212045	0.0
18	0.0	0.0	0.0	14.61385342646407	0.0
19	0.0	0.0	0.0	15.507947039923792	0.0
20	0.0	0.0	0.0	15.986861848426699	0.0
21	0.0	0.0	0.0	16.469218125803582	0.0
22	1.3765875495915663E-4	0.0	0.0	16.98709036195993	0.0
23	1.3765875495915663E-4	0.0	0.0	17.476054259574855	0.0
24	1.3765875495915663E-4	0.0	0.0	17.864664924824552	0.0
25	1.3765875495915663E-4	0.0	0.0	18.225330862817543	0.0
26	1.3765875495915663E-4	0.0	0.0	18.560116954878215	0.0
27	1.3765875495915663E-4	0.0	0.0	18.938678531015896	0.0
28	1.3765875495915663E-4	0.0	0.0	19.2898460149167	0.0
29	1.3765875495915663E-4	0.0	0.0	19.63302929102988	0.0
30	1.3765875495915663E-4	0.0	0.0	20.011315549657642	0.0
31	1.3765875495915663E-4	0.0	0.0	20.373082757690305	0.0
32	1.3765875495915663E-4	0.0	0.0	20.74131992720605	0.0
33	1.3765875495915663E-4	0.0	0.0	21.101435230179206	0.0
34	1.3765875495915663E-4	0.0	0.0	21.449436562715952	0.0
35	1.3765875495915663E-4	0.0	0.0	21.814369922112675	0.0
36	1.3765875495915663E-4	0.0	0.0	22.170906097456893	0.0
37	1.3765875495915663E-4	0.0	0.0	22.524413780192006	0.0
38	1.3765875495915663E-4	0.0	0.0	22.892100314687912	0.0
39	1.3765875495915663E-4	0.0	0.0	23.23679783710564	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	35	1.2111013E-7	45.000004	1
TGCGTAG	35	1.2111013E-7	45.000004	1
TGTGCGA	20	7.0315704E-4	45.0	1
TGTTGCG	25	3.8893828E-5	45.0	1
ACTAGCG	20	7.0315704E-4	45.0	1
CGTGTTC	20	7.0315704E-4	45.0	23
ATAACGG	20	7.0315704E-4	45.0	2
ACGTAAG	20	7.0315704E-4	45.0	1
TGTCGGA	20	7.0315704E-4	45.0	29
CGAACCC	120	0.0	44.999996	34
CTAGTCG	30	2.1643955E-6	44.999996	29
CGAATGC	370	0.0	43.175674	45
CGTTTTT	4325	0.0	43.07514	1
GATGAAT	2335	0.0	43.072803	20
ATCGAAT	430	0.0	42.383724	43
CGATGAA	2260	0.0	42.11283	19
CCGATGA	2165	0.0	41.882217	18
CCCATCG	480	0.0	41.718746	40
TGAATGA	2425	0.0	41.56701	22
GCGAACC	130	0.0	41.53846	33
>>END_MODULE