FastQCFastQC Report
Sat 18 Jun 2016
SRR3551264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692133
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46870.6771819866990882No Hit
GCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC16550.2391158924657544TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGCT11330.16369686173033215TruSeq Adapter, Index 13 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC10390.1501156569618845No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC9820.1418802455597407No Hit
CCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC9670.13971303203286073TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCG7750.11197269888879738No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7730.11168373708521338No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCC7600.10980548536191742No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC7370.10648242462070151No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7350.10619346281711753No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7210.10417073019202956No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA7200.10402624929023757No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG207.03134E-445.01
TTATGCG253.889191E-545.01
GTATGCG207.03134E-445.01
ACCACGC253.889191E-545.040
CGTCCAT253.889191E-545.016
CGACGGT650.041.5384628
CGGTCTA603.6379788E-1241.25000431
CGTTTTT20900.041.2320561
TATGCGG700.038.5714262
TGATTCG356.244687E-638.57142615
CGCGGTA301.1394526E-437.50000430
TCTAGCG301.1394526E-437.5000041
TCTCGAG301.1394526E-437.5000041
TCGAAAG301.1394526E-437.5000041
TCTACTC1750.037.28571328
CGGGTAT1150.037.1739126
TTGTGCG552.746674E-936.818181
CGAATAT1100.036.8181814
GCACCGA1850.036.4864859
CTCACGT751.8189894E-1236.045
Produced by FastQC (version 0.11.5)