##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551264_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	692133
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.637766151881213	33.0	31.0	34.0	30.0	34.0
2	31.77811489988196	33.0	31.0	34.0	30.0	34.0
3	31.758591195622806	33.0	31.0	34.0	30.0	34.0
4	35.46611272688919	37.0	35.0	37.0	33.0	37.0
5	34.262131700121216	37.0	35.0	37.0	32.0	37.0
6	34.74232120127201	37.0	35.0	37.0	32.0	37.0
7	35.48093068817698	37.0	35.0	37.0	33.0	37.0
8	35.2581555857039	37.0	35.0	37.0	32.0	37.0
9	37.16435280502447	39.0	37.0	39.0	34.0	39.0
10	36.70480240069467	39.0	35.0	39.0	32.0	39.0
11	36.853509368864074	39.0	37.0	39.0	33.0	39.0
12	36.852823084580564	39.0	37.0	39.0	33.0	39.0
13	36.97093766660454	39.0	37.0	39.0	33.0	39.0
14	37.99893373094478	40.0	37.0	41.0	33.0	41.0
15	38.13847627551352	40.0	37.0	41.0	33.0	41.0
16	38.18020091514203	40.0	37.0	41.0	34.0	41.0
17	38.108669865473836	40.0	37.0	41.0	34.0	41.0
18	38.01811068103963	40.0	37.0	41.0	33.0	41.0
19	37.96031687551381	40.0	37.0	41.0	34.0	41.0
20	38.10683206840304	40.0	37.0	41.0	34.0	41.0
21	37.94264686122465	40.0	36.0	41.0	34.0	41.0
22	38.06399059140367	40.0	37.0	41.0	34.0	41.0
23	38.16410140825535	40.0	37.0	41.0	34.0	41.0
24	38.03682529224874	40.0	37.0	41.0	34.0	41.0
25	37.715820514265324	40.0	36.0	41.0	33.0	41.0
26	37.85688299792092	40.0	36.0	41.0	33.0	41.0
27	37.95487861437036	40.0	36.0	41.0	34.0	41.0
28	37.85873957750895	40.0	36.0	41.0	34.0	41.0
29	37.89689842848123	40.0	36.0	41.0	34.0	41.0
30	37.61334743466935	40.0	36.0	41.0	33.0	41.0
31	37.56052810659223	40.0	36.0	41.0	33.0	41.0
32	37.617219522837374	40.0	36.0	41.0	33.0	41.0
33	37.605492008038915	40.0	36.0	41.0	33.0	41.0
34	37.47954222671076	40.0	36.0	41.0	33.0	41.0
35	37.47649512449197	40.0	36.0	41.0	33.0	41.0
36	37.301330813586404	40.0	36.0	41.0	33.0	41.0
37	37.344779110373295	40.0	36.0	41.0	33.0	41.0
38	37.13564589464742	40.0	35.0	41.0	32.0	41.0
39	37.082167733658125	40.0	35.0	41.0	32.0	41.0
40	36.98241522944289	39.0	35.0	41.0	32.0	41.0
41	36.844765384687626	39.0	35.0	41.0	31.0	41.0
42	36.97632391462334	39.0	35.0	41.0	32.0	41.0
43	36.92152230857364	39.0	35.0	41.0	32.0	41.0
44	36.97320168233562	39.0	35.0	41.0	32.0	41.0
45	36.93978613936917	39.0	35.0	41.0	32.0	41.0
46	36.862366048143926	39.0	35.0	41.0	32.0	41.0
47	36.83306965568756	39.0	35.0	41.0	32.0	41.0
48	36.76441811039208	39.0	35.0	41.0	32.0	41.0
49	36.732646760088016	39.0	35.0	41.0	32.0	41.0
50	36.6520278616971	39.0	35.0	41.0	32.0	41.0
51	35.753973586001536	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	17.0
9	28.0
10	49.0
11	32.0
12	38.0
13	41.0
14	61.0
15	83.0
16	91.0
17	144.0
18	224.0
19	352.0
20	532.0
21	925.0
22	1302.0
23	1863.0
24	2736.0
25	3944.0
26	5483.0
27	6628.0
28	7009.0
29	7757.0
30	9362.0
31	12180.0
32	16066.0
33	23094.0
34	41878.0
35	51497.0
36	56105.0
37	85500.0
38	146572.0
39	210476.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.14796867076125	21.80780283558218	22.85153287012756	13.192695623529005
2	35.01725824371905	24.40080158004314	23.648922967117592	16.933017209120212
3	32.62552139545434	23.535360978309082	26.908556592446825	16.930561033789747
4	29.202624351100148	25.46028003288385	26.456764812543256	18.88033080347274
5	24.99273983468495	32.3554865900051	23.52727004780873	19.124503527501215
6	26.305059865661658	34.77424136690492	24.22858034510708	14.692118422326345
7	75.85969748588782	7.332116804140244	11.421359767559125	5.386825942412802
8	76.72773874385415	7.126953923595609	10.457672152606508	5.687635179943739
9	69.7078451684864	9.130326107843436	13.575425532376004	7.586403191294158
10	38.74342070093464	25.73060380013668	20.423386834611268	15.102588664317407
11	29.557324964999506	25.36087717245096	26.108854801028127	18.972943061521413
12	27.52317834866998	22.316231128988214	29.68677985300513	20.47381066933667
13	24.593539103033667	24.014748610454927	30.885682376075117	20.50602991043629
14	20.542728059491456	26.940920314448235	30.490382628772217	22.025968997288093
15	20.231371716129704	26.25405810732908	33.55438911307509	19.960181063466127
16	21.897958918300382	25.738550249735237	31.594505680266654	20.768985151697724
17	21.83106426077069	25.941545916752993	29.225885776288663	23.001504046187655
18	22.701272732263885	25.355386898182864	30.152730761284317	21.790609608268934
19	23.68056428461004	27.05578263137287	27.959510672081812	21.304142411935278
20	24.494136242600774	26.143674698359998	29.338869841490002	20.02331921754923
21	24.043500309911533	26.33987976299353	29.391894332447666	20.22472559464727
22	22.232865648654233	25.424159807435853	28.89343522126528	23.449539322644636
23	22.353362720748756	25.524285072377705	29.848020539405002	22.274331667468537
24	22.40985475334943	24.85432713076822	30.063586044878658	22.672232071003695
25	22.272308934843448	26.35461681497631	28.20628405234254	23.1667901978377
26	20.538104670634112	27.002469178611626	29.35837476323192	23.10105138752234
27	21.569264866723593	26.44824043933753	29.742260519293257	22.24023417464562
28	20.220680129397095	26.299714072295355	30.8281789771619	22.651426821145648
29	21.900415093630848	25.196168944408083	30.002181661617062	22.901234300344008
30	21.50251469009569	26.007284727068352	29.650370665753545	22.83982991708241
31	22.607071184295506	26.1129002662783	27.87412245912274	23.405906090303453
32	23.170980143989667	27.078321652052423	27.977426303904018	21.773271900053892
33	22.484984822281266	27.08164471279364	27.54759562107283	22.885774843852268
34	20.626093539825437	25.995003850416033	29.560214583035343	23.818688026723187
35	22.2803998653438	24.78699903053315	29.09917602541708	23.83342507870597
36	21.94751586761504	26.999290598772202	28.437309014307942	22.615884519304817
37	22.717021150559212	26.800773839709997	28.470973064425483	22.01123194530531
38	21.817483056002242	27.16501019312762	27.883947160444595	23.13355959042554
39	23.540562290773597	25.044175035722905	28.41318070370868	23.002081969794823
40	22.239511770136662	24.811849745641375	30.51523334388044	22.433405140341524
41	20.73849968141961	24.925122772646297	29.88934207731751	24.447035468616583
42	21.41727095803841	25.113670349484853	30.041769428708065	23.42728926376867
43	22.50998001829128	24.624169054213567	29.48176145336229	23.384089474132864
44	21.981324398634367	25.004876230435478	28.297018058667916	24.71678131226224
45	21.612753618162984	24.860973252250652	28.37272605120692	25.153547078379447
46	21.672135268799494	26.114778518001597	28.50694880897169	23.706137404227224
47	21.601195146019624	24.72183814382496	30.43042305452854	23.24654365562688
48	20.721450935008157	24.153450276175246	30.62128232579576	24.503816463020836
49	21.53935732005265	24.678060430581983	30.326541286140092	23.456040963225274
50	20.363860703072962	23.821144202053652	31.087522195878535	24.727472898994847
51	20.36010419962637	23.537239230032377	29.0988870636135	27.003769506727753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	287.0
1	692.0
2	1097.0
3	4474.5
4	7852.0
5	5122.5
6	2393.0
7	2333.0
8	2273.0
9	2208.0
10	2143.0
11	2063.0
12	1983.0
13	1991.0
14	1999.0
15	1900.0
16	1801.0
17	1841.0
18	1881.0
19	1835.0
20	1789.0
21	2320.5
22	2852.0
23	3004.0
24	3156.0
25	3660.0
26	5643.5
27	7123.0
28	8758.5
29	10394.0
30	11312.5
31	12231.0
32	14062.0
33	15893.0
34	17028.5
35	18164.0
36	19621.5
37	21079.0
38	22960.0
39	24841.0
40	28222.0
41	31603.0
42	34529.5
43	37456.0
44	41302.0
45	45148.0
46	52138.0
47	59128.0
48	61779.0
49	64430.0
50	62800.5
51	61171.0
52	54365.0
53	47559.0
54	42538.0
55	37517.0
56	35486.5
57	33456.0
58	31643.0
59	29830.0
60	28494.0
61	27158.0
62	25244.5
63	23331.0
64	20335.5
65	17340.0
66	14439.5
67	11539.0
68	9551.0
69	7563.0
70	6314.5
71	5066.0
72	4069.0
73	3072.0
74	2541.5
75	1644.5
76	1278.0
77	941.5
78	605.0
79	417.0
80	229.0
81	187.0
82	145.0
83	107.5
84	70.0
85	47.0
86	24.0
87	13.5
88	3.0
89	3.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	692133.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.489493198956314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.12508080400457	23.341612278497887
2	9.246703472070887	5.823480121930839
3	3.2215301135512977	3.043330518027197
4	1.6816316208847868	2.118149099560055
5	1.108254435943445	1.7449185261677138
6	0.8455515444028875	1.597559376410504
7	0.709245284060797	1.5633642184177017
8	0.5987702963710553	1.5083978540250722
9	0.5748922899711054	1.629276016965979
>10	7.732579643818262	50.169316190713396
>50	0.10089560417445147	2.0948701762152604
>100	0.047425583526603456	2.823272407488179
>500	0.005579480414894524	1.2958971155254821
>1k	0.0018598268049648413	1.2465561000546779
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4687	0.6771819866990882	No Hit
GCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	1655	0.2391158924657544	TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGCT	1133	0.16369686173033215	TruSeq Adapter, Index 13 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTC	1039	0.1501156569618845	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	982	0.1418802455597407	No Hit
CCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCGTCTTCTGC	967	0.13971303203286073	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCCG	775	0.11197269888879738	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	773	0.11168373708521338	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGCC	760	0.10980548536191742	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGACTGTCGTATGC	737	0.10648242462070151	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	735	0.10619346281711753	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	721	0.10417073019202956	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	720	0.10402624929023757	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4448090179199663E-4	0.0	0.0	0.16904265509663605	0.0
2	1.4448090179199663E-4	0.0	0.0	0.7120018840309593	0.0
3	1.4448090179199663E-4	0.0	0.0	0.9996633594988247	0.0
4	1.4448090179199663E-4	0.0	0.0	1.4039209227128313	0.0
5	1.4448090179199663E-4	0.0	0.0	2.5152680192968693	0.0
6	1.4448090179199663E-4	0.0	0.0	3.3288399772876023	0.0
7	1.4448090179199663E-4	0.0	0.0	4.036940876970178	0.0
8	1.4448090179199663E-4	0.0	0.0	5.102776489489737	0.0
9	1.4448090179199663E-4	0.0	0.0	5.630131781030524	0.0
10	1.4448090179199663E-4	0.0	0.0	6.671694602049028	0.0
11	1.4448090179199663E-4	0.0	0.0	7.896748168343367	0.0
12	1.4448090179199663E-4	0.0	0.0	8.9468353625676	0.0
13	1.4448090179199663E-4	0.0	0.0	9.40787392018586	0.0
14	1.4448090179199663E-4	0.0	0.0	9.601911771292512	0.0
15	1.4448090179199663E-4	0.0	0.0	9.96065785044204	0.0
16	1.4448090179199663E-4	0.0	0.0	10.580914361835081	0.0
17	1.4448090179199663E-4	0.0	0.0	11.267054164445273	0.0
18	1.4448090179199663E-4	0.0	0.0	12.031213654023142	0.0
19	1.4448090179199663E-4	0.0	0.0	12.513924346910203	0.0
20	1.4448090179199663E-4	0.0	0.0	12.994034383565008	0.0
21	1.4448090179199663E-4	0.0	0.0	13.56430050293802	0.0
22	1.4448090179199663E-4	0.0	0.0	14.161729031847925	0.0
23	1.4448090179199663E-4	0.0	0.0	14.707866840621673	0.0
24	1.4448090179199663E-4	0.0	0.0	15.150411842810557	0.0
25	1.4448090179199663E-4	0.0	0.0	15.57677498399874	0.0
26	1.4448090179199663E-4	0.0	0.0	15.974964349337483	0.0
27	1.4448090179199663E-4	0.0	0.0	16.361595242532864	0.0
28	1.4448090179199663E-4	0.0	0.0	16.77741127789023	0.0
29	1.4448090179199663E-4	0.0	0.0	17.19539452677448	0.0
30	1.4448090179199663E-4	0.0	0.0	17.668424999241477	0.0
31	1.4448090179199663E-4	0.0	0.0	18.10432387994793	0.0
32	1.4448090179199663E-4	0.0	0.0	18.54224549327947	0.0
33	1.4448090179199663E-4	0.0	0.0	18.966152459137188	0.0
34	1.4448090179199663E-4	0.0	0.0	19.391937676718204	0.0
35	2.8896180358399325E-4	0.0	0.0	19.825380382094192	0.0
36	2.8896180358399325E-4	0.0	0.0	20.245530844505318	0.0
37	2.8896180358399325E-4	0.0	0.0	20.682007648818942	0.0
38	2.8896180358399325E-4	0.0	0.0	21.108515270908914	0.0
39	2.8896180358399325E-4	0.0	0.0	21.550626830392424	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACG	20	7.03134E-4	45.0	1
TTATGCG	25	3.889191E-5	45.0	1
GTATGCG	20	7.03134E-4	45.0	1
ACCACGC	25	3.889191E-5	45.0	40
CGTCCAT	25	3.889191E-5	45.0	16
CGACGGT	65	0.0	41.53846	28
CGGTCTA	60	3.6379788E-12	41.250004	31
CGTTTTT	2090	0.0	41.232056	1
TATGCGG	70	0.0	38.571426	2
TGATTCG	35	6.244687E-6	38.571426	15
CGCGGTA	30	1.1394526E-4	37.500004	30
TCTAGCG	30	1.1394526E-4	37.500004	1
TCTCGAG	30	1.1394526E-4	37.500004	1
TCGAAAG	30	1.1394526E-4	37.500004	1
TCTACTC	175	0.0	37.285713	28
CGGGTAT	115	0.0	37.173912	6
TTGTGCG	55	2.746674E-9	36.81818	1
CGAATAT	110	0.0	36.81818	14
GCACCGA	185	0.0	36.486485	9
CTCACGT	75	1.8189894E-12	36.0	45
>>END_MODULE