FastQCFastQC Report
Sat 18 Jun 2016
SRR3551263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668125
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74991.1223947614593077No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC34420.5151730589335828TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT21990.32913002806361086TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC21750.3255378858746492TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCG18500.2768942937324602No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC18190.27225444340505145No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGC17330.2593826005612722No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCC13210.19771749298409727No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC13030.19502338634237604TruSeq Adapter, Index 16 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG8770.13126286248830685No Hit
ACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC8590.12856875584658561TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC7960.11913938260056127No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTT7270.10881197380729654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG302.1641317E-645.0000041
CGCATCG207.0311624E-445.00000421
ATAGACG207.0311624E-445.0000041
GTATGCG302.1641317E-645.0000041
TGCGACT302.1641317E-645.00000433
GTATACG207.0311624E-445.0000041
CGAGAGT207.0311624E-445.0000042
TAGGCCC207.0311624E-445.0000046
CATCCGA207.0311624E-445.00000426
CGTTTGG302.1641317E-645.0000042
CAATACG207.0311624E-445.00000441
GTCATCG253.889047E-544.99999618
CGTTTTT36200.043.508291
CGCACGG603.6379788E-1241.2500042
GCGATAT501.0804797E-940.4999969
CGTTATT1400.040.1785741
GCGATAC451.9263098E-840.09
CGTCTGG403.455316E-739.3750042
TACGAAT800.039.37500412
TGCGACG356.2443614E-638.571431
Produced by FastQC (version 0.11.5)