##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551263_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	668125
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51019794200187	31.0	31.0	34.0	30.0	34.0
2	31.65706267539757	31.0	31.0	34.0	30.0	34.0
3	31.624525350795135	33.0	31.0	34.0	30.0	34.0
4	35.36892198316183	37.0	35.0	37.0	33.0	37.0
5	34.201987652011226	37.0	35.0	37.0	32.0	37.0
6	34.68967932647334	37.0	35.0	37.0	32.0	37.0
7	35.425812535079515	37.0	35.0	37.0	33.0	37.0
8	35.26833601496726	37.0	35.0	37.0	33.0	37.0
9	37.14082394761459	39.0	37.0	39.0	34.0	39.0
10	36.58275771749298	38.0	35.0	39.0	32.0	39.0
11	36.77959513564078	39.0	37.0	39.0	33.0	39.0
12	36.82766847521048	39.0	37.0	39.0	33.0	39.0
13	36.9439251637044	39.0	37.0	39.0	33.0	39.0
14	37.934742750233866	40.0	37.0	41.0	33.0	41.0
15	38.064905519176804	40.0	37.0	41.0	33.0	41.0
16	38.099405051449956	40.0	37.0	41.0	33.0	41.0
17	38.05300804490178	40.0	37.0	41.0	33.0	41.0
18	37.949831244153415	39.0	37.0	41.0	33.0	41.0
19	37.81789934518241	39.0	37.0	41.0	33.0	41.0
20	37.911411786716556	39.0	36.0	41.0	34.0	41.0
21	37.74980729653882	39.0	36.0	41.0	33.0	41.0
22	37.894642469597756	40.0	36.0	41.0	34.0	41.0
23	37.98849167446211	40.0	36.0	41.0	34.0	41.0
24	37.83875023386342	40.0	36.0	41.0	33.0	41.0
25	37.51989223573433	39.0	35.0	41.0	33.0	41.0
26	37.69354686623012	39.0	36.0	41.0	33.0	41.0
27	37.773274462114124	40.0	36.0	41.0	33.0	41.0
28	37.67871730589336	40.0	36.0	41.0	33.0	41.0
29	37.6960613657624	40.0	36.0	41.0	33.0	41.0
30	37.395918428437795	39.0	36.0	41.0	33.0	41.0
31	37.302521234798874	39.0	35.0	41.0	33.0	41.0
32	37.304227502338634	40.0	36.0	41.0	33.0	41.0
33	37.227753788587464	40.0	36.0	41.0	33.0	41.0
34	37.054250327408795	40.0	36.0	41.0	32.0	41.0
35	37.022693358278765	40.0	36.0	41.0	32.0	41.0
36	36.83319588400374	40.0	35.0	41.0	31.0	41.0
37	36.82738709073901	40.0	35.0	41.0	31.0	41.0
38	36.63069485500468	39.0	35.0	41.0	31.0	41.0
39	36.6023693171188	39.0	35.0	41.0	31.0	41.0
40	36.48624733395697	39.0	35.0	41.0	30.0	41.0
41	36.3668220767072	39.0	35.0	41.0	30.0	41.0
42	36.47878765201123	39.0	35.0	41.0	30.0	41.0
43	36.41805500467727	39.0	35.0	41.0	30.0	41.0
44	36.4631812909261	39.0	35.0	41.0	31.0	41.0
45	36.462992703461175	39.0	35.0	41.0	30.0	41.0
46	36.397195135640786	39.0	35.0	41.0	30.0	41.0
47	36.35942825070159	39.0	35.0	41.0	30.0	41.0
48	36.32703610851263	39.0	35.0	41.0	30.0	41.0
49	36.311642282507016	39.0	35.0	41.0	30.0	41.0
50	36.20820954162769	39.0	35.0	41.0	30.0	41.0
51	35.32500205799813	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	28.0
10	34.0
11	20.0
12	34.0
13	31.0
14	50.0
15	57.0
16	75.0
17	136.0
18	250.0
19	320.0
20	654.0
21	1031.0
22	1522.0
23	2326.0
24	3545.0
25	5470.0
26	7720.0
27	8654.0
28	8552.0
29	8770.0
30	10187.0
31	12340.0
32	16368.0
33	23138.0
34	38824.0
35	50400.0
36	55604.0
37	82467.0
38	140045.0
39	189423.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.37178671655753	21.20112254443405	23.756482694106644	14.670608044901778
2	36.07528531337699	24.005537885874652	23.302525724976615	16.61665107577175
3	29.898896164639847	22.767146866230124	30.914424695977548	16.41953227315248
4	27.407895229186156	24.66020579981291	29.73934518241347	18.192553788587464
5	24.060318054256314	30.116370439663235	27.37646398503274	18.446847521047708
6	24.047895229186157	33.20112254443405	28.037867165575303	14.71311506080449
7	73.6065855940131	6.053956969130028	14.926548175865296	5.412909260991581
8	73.94035547240412	6.234462114125351	13.239887745556594	6.585294667913938
9	67.78791393826006	7.497698783910196	16.40426566884939	8.310121608980356
10	36.09803554724041	24.04115996258185	23.88115996258185	15.979644527595886
11	26.540542563143127	24.109859681945743	28.727857811038355	20.62173994387278
12	23.839101964452762	20.809279700654816	34.22503274087932	21.126585594013097
13	22.805912067352665	23.988924228250703	34.81444340505145	18.39072029934518
14	19.232179607109448	27.58869971936389	32.270757717492984	20.908362956033677
15	18.2422450888681	25.57650140318054	36.18289990645463	19.998353601496728
16	20.079925163704395	25.546866230121605	33.07644527595884	21.296763330215153
17	20.236632366697847	25.659569691300284	30.49758652946679	23.60621141253508
18	21.021216089803556	25.165350795135637	32.62563143124415	21.18780168381665
19	21.25156220767072	27.242357343311507	29.987502338634236	21.518578110383537
20	21.320561272217027	26.474087932647333	31.74825070159027	20.457100093545368
21	21.151431244153414	27.69601496725912	31.797193638914873	19.35536014967259
22	19.466716557530404	24.660355472404117	30.80950420954163	25.063423760523857
23	19.121122544434048	26.0910757717493	30.969504209541626	23.818297474275024
24	20.616800748362955	25.473526660430306	31.068437792329277	22.841234798877455
25	18.771188026192704	27.82772684752105	29.497174929840973	23.903910196445278
26	17.98465855940131	26.851262862488305	31.13534144059869	24.028737137511694
27	20.6666417212348	25.319513564078576	31.938185219831617	22.075659494855003
28	18.108138447146864	24.751955098222638	33.733657623947614	23.406248830682884
29	18.886286248830682	23.16138447146866	31.74929840972872	26.20303086997194
30	19.51446211412535	24.9577549111319	32.545406922357344	22.982376052385405
31	19.96557530402245	25.097848456501403	30.06593077642657	24.870645463049577
32	18.99135640785781	25.05070159027128	30.57736202057998	25.380579981290925
33	20.011225444340504	23.417923292797006	31.204939195509823	25.365912067352664
34	17.334181478016838	23.2911506080449	31.767857811038354	27.60681010289991
35	18.29268475210477	22.45942001870907	31.740168381665107	27.507726847521045
36	21.131524789522917	23.31405051449953	32.5754911131899	22.978933582787654
37	18.621066417212347	24.499607109448082	33.219831618334894	23.659494855004677
38	18.53754911131899	25.564826941066414	31.99072029934518	23.906903648269413
39	21.50196445275959	22.602507015902713	30.76041159962582	25.13511693171188
40	20.671431244153414	21.99528531337699	32.642544434050514	24.690739008419083
41	19.121122544434048	22.988811973807298	30.57481758652947	27.315247895229188
42	20.731150608044903	22.89017773620206	32.202357343311505	24.176314312441534
43	21.991244153414407	22.222039289055193	31.62162768942937	24.16508886810103
44	20.063910196445274	23.27708138447147	30.78645463049579	25.872553788587467
45	19.943872778297475	22.159176800748362	30.346267539756784	27.550682881197382
46	21.411861552853136	23.113189897100096	31.04793264733396	24.427015902712814
47	18.6022076707203	22.68018709073901	33.96205799812909	24.755547240411598
48	19.39397567820393	21.918054256314313	32.9733208606174	25.714649204864358
49	20.740729653882134	20.44617399438728	34.04692235734331	24.766173994387277
50	19.480935453695043	20.386155285313375	33.33942001870908	26.793489242282508
51	19.2844153414406	20.336763330215156	30.96127221702526	29.41754911131899
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	245.0
1	529.5
2	814.0
3	6049.0
4	11284.0
5	7374.0
6	3464.0
7	3296.0
8	3128.0
9	3180.5
10	3233.0
11	3198.5
12	3164.0
13	3127.0
14	3090.0
15	2993.0
16	2896.0
17	2881.0
18	2866.0
19	2645.0
20	2424.0
21	2465.5
22	2507.0
23	2469.0
24	2431.0
25	3242.0
26	4650.5
27	5248.0
28	6035.5
29	6823.0
30	7980.5
31	9138.0
32	10087.0
33	11036.0
34	12748.0
35	14460.0
36	16357.0
37	18254.0
38	19326.0
39	20398.0
40	24537.5
41	28677.0
42	32401.0
43	36125.0
44	41887.0
45	47649.0
46	60214.5
47	72780.0
48	77401.0
49	82022.0
50	78155.5
51	74289.0
52	62331.0
53	50373.0
54	42454.0
55	34535.0
56	30504.5
57	26474.0
58	23514.5
59	20555.0
60	19188.0
61	17821.0
62	15840.0
63	13859.0
64	12284.5
65	10710.0
66	9444.0
67	8178.0
68	6514.5
69	4851.0
70	4061.0
71	3271.0
72	2782.5
73	2294.0
74	1756.5
75	949.0
76	679.0
77	578.5
78	478.0
79	336.0
80	194.0
81	140.5
82	87.0
83	57.0
84	27.0
85	18.5
86	10.0
87	5.5
88	1.0
89	1.5
90	2.0
91	3.5
92	5.0
93	4.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	668125.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.549692793277934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.74971863001998	28.06828970941214
2	10.568147280935564	7.936613675864917
3	3.79789491966565	4.278293624837884
4	1.9095705440032358	2.868151491776565
5	1.2641269295526107	2.373378892820511
6	0.9174441144483527	2.066984679152194
7	0.7128595371482521	1.8737359637272613
8	0.6021120969501093	1.8087299414074387
9	0.5035292632573498	1.701663223296515
>10	4.808847591892771	36.31503652426008
>50	0.10981897981935328	2.732812135211911
>100	0.048989879392792646	3.532711929881032
>500	0.0032659919595195096	0.8655105904147672
>1k	0.0032659919595195096	2.428519088443277
>5k	4.082489949399387E-4	1.1495685294935065
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7499	1.1223947614593077	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	3442	0.5151730589335828	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT	2199	0.32913002806361086	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	2175	0.3255378858746492	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCG	1850	0.2768942937324602	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC	1819	0.27225444340505145	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGC	1733	0.2593826005612722	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCC	1321	0.19771749298409727	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	1303	0.19502338634237604	TruSeq Adapter, Index 16 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG	877	0.13126286248830685	No Hit
ACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	859	0.12856875584658561	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC	796	0.11913938260056127	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTT	727	0.10881197380729654	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.34005612722170253	0.0
2	0.0	0.0	0.0	1.5509073900841908	0.0
3	0.0	0.0	0.0	2.1394200187090737	0.0
4	0.0	0.0	0.0	2.9361272217025256	0.0
5	0.0	0.0	0.0	4.941290926099158	0.0
6	0.0	0.0	0.0	6.3167820392890555	0.0
7	0.0	0.0	0.0	7.613096351730589	0.0
8	0.0	0.0	0.0	9.648942937324602	0.0
9	0.0	0.0	0.0	10.500130963517305	0.0
10	0.0	0.0	0.0	12.385257249766136	0.0
11	0.0	0.0	0.0	14.505818521983162	0.0
12	0.0	0.0	0.0	16.30533208606174	0.0
13	0.0	0.0	0.0	16.997268475210475	0.0
14	0.0	0.0	0.0	17.27461178671656	0.0
15	0.0	0.0	0.0	17.76733395696913	0.0
16	0.0	0.0	0.0	18.73885874649205	0.0
17	0.0	0.0	0.0	19.937885874649204	0.0
18	0.0	0.0	0.0	21.28329279700655	0.0
19	0.0	0.0	0.0	22.108737137511692	0.0
20	1.4967259120673526E-4	0.0	0.0	22.849616463985033	0.0
21	1.4967259120673526E-4	0.0	0.0	23.701253507951357	0.0
22	1.4967259120673526E-4	0.0	0.0	24.595247895229186	0.0
23	1.4967259120673526E-4	0.0	0.0	25.520523854069225	0.0
24	2.993451824134705E-4	0.0	0.0	26.23027128157156	0.0
25	2.993451824134705E-4	0.0	0.0	26.855453695042094	0.0
26	2.993451824134705E-4	0.0	0.0	27.43199251637044	0.0
27	2.993451824134705E-4	0.0	0.0	28.040561272217026	0.0
28	2.993451824134705E-4	0.0	0.0	28.646735266604303	0.0
29	2.993451824134705E-4	0.0	0.0	29.25530402245089	0.0
30	2.993451824134705E-4	0.0	0.0	29.926136576239475	0.0
31	2.993451824134705E-4	0.0	0.0	30.55640785781104	0.0
32	2.993451824134705E-4	0.0	0.0	31.159289055191767	0.0
33	2.993451824134705E-4	0.0	0.0	31.746604303086997	0.0
34	2.993451824134705E-4	0.0	0.0	32.3066791393826	0.0
35	2.993451824134705E-4	0.0	0.0	32.896838166510754	0.0
36	2.993451824134705E-4	0.0	0.0	33.44628624883068	0.0
37	2.993451824134705E-4	0.0	0.0	34.01354536950421	0.0
38	2.993451824134705E-4	0.0	0.0	34.57152478952292	0.0
39	2.993451824134705E-4	0.0	0.0	35.142525724976615	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	30	2.1641317E-6	45.000004	1
CGCATCG	20	7.0311624E-4	45.000004	21
ATAGACG	20	7.0311624E-4	45.000004	1
GTATGCG	30	2.1641317E-6	45.000004	1
TGCGACT	30	2.1641317E-6	45.000004	33
GTATACG	20	7.0311624E-4	45.000004	1
CGAGAGT	20	7.0311624E-4	45.000004	2
TAGGCCC	20	7.0311624E-4	45.000004	6
CATCCGA	20	7.0311624E-4	45.000004	26
CGTTTGG	30	2.1641317E-6	45.000004	2
CAATACG	20	7.0311624E-4	45.000004	41
GTCATCG	25	3.889047E-5	44.999996	18
CGTTTTT	3620	0.0	43.50829	1
CGCACGG	60	3.6379788E-12	41.250004	2
GCGATAT	50	1.0804797E-9	40.499996	9
CGTTATT	140	0.0	40.178574	1
GCGATAC	45	1.9263098E-8	40.0	9
CGTCTGG	40	3.455316E-7	39.375004	2
TACGAAT	80	0.0	39.375004	12
TGCGACG	35	6.2443614E-6	38.57143	1
>>END_MODULE