##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551262_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	680717
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57395070198041	31.0	31.0	34.0	30.0	34.0
2	31.74249651470435	33.0	31.0	34.0	30.0	34.0
3	31.695255150084396	33.0	31.0	34.0	30.0	34.0
4	35.418654154369584	37.0	35.0	37.0	33.0	37.0
5	34.24289682790352	37.0	35.0	37.0	32.0	37.0
6	34.724966469178824	37.0	35.0	37.0	32.0	37.0
7	35.43760476086244	37.0	35.0	37.0	33.0	37.0
8	35.2459597747669	37.0	35.0	37.0	32.0	37.0
9	37.17259007781501	39.0	37.0	39.0	34.0	39.0
10	36.7614911923751	39.0	37.0	39.0	32.0	39.0
11	36.88449678794565	39.0	37.0	39.0	33.0	39.0
12	36.91406854831009	39.0	37.0	39.0	33.0	39.0
13	37.041930787684166	39.0	37.0	39.0	33.0	39.0
14	38.10481007525888	40.0	37.0	41.0	33.0	41.0
15	38.19846132827592	40.0	37.0	41.0	33.0	41.0
16	38.24665022322051	40.0	38.0	41.0	34.0	41.0
17	38.13195057564303	40.0	37.0	41.0	33.0	41.0
18	37.96997577554255	39.0	37.0	41.0	33.0	41.0
19	37.84382937402768	39.0	37.0	41.0	34.0	41.0
20	37.818044796883285	39.0	36.0	41.0	34.0	41.0
21	37.71098562251273	39.0	36.0	41.0	33.0	41.0
22	37.8274055150672	40.0	36.0	41.0	33.0	41.0
23	37.88758177039798	40.0	36.0	41.0	34.0	41.0
24	37.782292788339355	40.0	35.0	41.0	33.0	41.0
25	37.467974209546696	39.0	35.0	41.0	33.0	41.0
26	37.59427926730198	39.0	35.0	41.0	33.0	41.0
27	37.63421509966697	40.0	35.0	41.0	33.0	41.0
28	37.492803911170135	39.0	35.0	41.0	33.0	41.0
29	37.47209780275797	40.0	35.0	41.0	33.0	41.0
30	37.180876928297664	39.0	35.0	41.0	32.0	41.0
31	37.034963134459694	39.0	35.0	41.0	32.0	41.0
32	36.94301743602701	39.0	35.0	41.0	32.0	41.0
33	36.75428408575076	40.0	35.0	41.0	31.0	41.0
34	36.463606755817764	40.0	35.0	41.0	30.0	41.0
35	36.36993052913325	40.0	35.0	41.0	30.0	41.0
36	36.15381869411224	39.0	35.0	41.0	29.0	41.0
37	36.126012718941936	39.0	35.0	41.0	29.0	41.0
38	35.91749287883217	39.0	35.0	41.0	27.0	41.0
39	35.88146762898532	39.0	35.0	41.0	27.0	41.0
40	35.756077782690895	39.0	35.0	41.0	26.0	41.0
41	35.57661700824278	39.0	35.0	41.0	26.0	41.0
42	35.70290884464469	39.0	35.0	41.0	26.0	41.0
43	35.652033076888046	39.0	35.0	41.0	26.0	41.0
44	35.71310397713	39.0	35.0	41.0	26.0	41.0
45	35.72157005040274	39.0	35.0	41.0	26.0	41.0
46	35.638636907848635	39.0	35.0	41.0	26.0	41.0
47	35.54271452013098	39.0	35.0	41.0	26.0	41.0
48	35.504460737722134	39.0	35.0	41.0	26.0	41.0
49	35.4924954129249	39.0	35.0	41.0	26.0	41.0
50	35.3729391215439	39.0	35.0	41.0	26.0	41.0
51	34.527214686866934	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	16.0
9	30.0
10	23.0
11	28.0
12	28.0
13	30.0
14	40.0
15	55.0
16	95.0
17	161.0
18	274.0
19	588.0
20	1054.0
21	1616.0
22	2489.0
23	3516.0
24	5078.0
25	7683.0
26	10227.0
27	11599.0
28	10968.0
29	10538.0
30	11428.0
31	13349.0
32	16814.0
33	22921.0
34	37490.0
35	48765.0
36	55352.0
37	80773.0
38	140000.0
39	187643.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.73281113884331	20.371901979824216	23.057893368316055	16.837393513016423
2	36.53309672007604	26.143463436347265	21.682725714210164	15.640714129366536
3	28.977533982550753	25.517065094598784	30.390749753568663	15.114651169281801
4	26.277880528912895	22.741462311063188	33.96418776084628	17.01646939917763
5	25.58317186143434	28.92890878294504	28.228911574119643	17.259007781500976
6	23.441753327741193	33.92393608503975	28.239488656813332	14.394821930405735
7	71.56072200341698	5.594836033182659	18.347272067540548	4.497169895859806
8	72.21165330085778	7.754323749810861	14.926320335763613	5.107702613567753
9	66.32403774255674	7.862738847421174	16.67565229015876	9.13757111986332
10	33.66700111793888	31.254397936293643	22.52360378835845	12.554997157409026
11	25.996412606119723	23.279865200957225	32.814223825760195	17.90949836716286
12	23.196717578670725	20.787493187330416	35.8764361695095	20.139353064489356
13	23.474365999380066	22.2325871103557	37.24117364484801	17.05187324541623
14	18.70689287912598	27.782323638163874	33.883831313159504	19.626952169550634
15	17.589688519605062	25.043446836203593	37.995525306404865	19.37133933778648
16	20.382038350739002	25.953369755713464	32.920435364475985	20.744156529071553
17	20.198702250715055	26.16461760173464	32.17680768953912	21.459872458011187
18	21.263755716399032	24.346681513756817	33.63042204028987	20.75914072955428
19	20.999181745130503	26.132004930095764	31.225310958886	21.64350236588773
20	22.289145121981672	25.94778740651401	33.07159950463996	18.69146796686435
21	22.285619427750444	27.10289297902065	32.90589187577216	17.70559571745674
22	20.5005898192641	23.778016415044725	33.091725342543235	22.629668423147944
23	19.648106334938014	26.326211920666005	32.99447494333181	21.031206801064172
24	20.478848038171517	25.546739687711632	32.65483306572335	21.319579208393503
25	19.861263932001112	28.79037838044297	30.535450120975383	20.81290756658053
26	19.3497444606202	26.834058793889383	31.961299629655205	21.854897115835215
27	22.39976377848651	25.465208008614447	31.248668683167896	20.886359529731152
28	19.147751561955996	26.729022486583997	33.01004675951974	21.11317919194026
29	20.506906688095054	25.174925850243202	32.23777281895413	22.080394642707617
30	22.936403821264932	26.345897046790366	31.428185281108007	19.28951385083669
31	23.6920776181585	26.8002708908401	28.10433704461619	21.403314446385206
32	22.810213348572166	27.789815738405238	28.42164952542687	20.978321387595727
33	22.42106484780019	25.710537565537518	29.57734271363871	22.29105487302359
34	21.17825763129171	28.386098775261964	29.317910379790717	21.1177332136556
35	21.549924564833844	28.331891226456808	28.70693695030387	21.411247258405474
36	24.194048334329832	29.14089114859773	27.76645801412334	18.898602502949096
37	22.834599400338174	28.066288927704168	28.480704903799964	20.618406768157694
38	21.387155014492073	27.740015307389122	28.440453227993423	22.432376450125382
39	22.897180472942498	26.41215071755223	28.725446845017828	21.965221964487444
40	21.32751202041377	26.828182636837333	31.21914099398135	20.625164348767548
41	20.53276177912407	28.882340238307545	28.168534060409833	22.416363922158546
42	22.90290972606825	27.736930324936793	28.972833056909113	20.387326892085845
43	23.959589667953054	25.93794484345183	28.348050658349944	21.754414830245167
44	22.523016172653247	25.30082251508336	28.956379817163374	23.21978149510002
45	21.75940956373941	24.49285092042655	28.81241396938816	24.93532554644588
46	23.37535275305303	26.790722135630517	28.243601966749765	21.590323144566685
47	20.482373732402746	26.040924495788996	31.9617403414341	21.51496143037415
48	21.266399987072454	25.61020218387377	30.163930091359553	22.959467737694226
49	21.977121182517845	23.639192204690055	31.93852952107851	22.44515709171359
50	21.169149587861035	23.442340943446396	32.102033591051786	23.28647587764078
51	20.872697464585137	23.348763142392507	29.620679371897573	26.157860021124783
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	256.0
1	539.0
2	822.0
3	8883.5
4	16945.0
5	11064.0
6	5183.0
7	4990.0
8	4797.0
9	4780.0
10	4763.0
11	4738.5
12	4714.0
13	4680.0
14	4646.0
15	4375.5
16	4105.0
17	3956.5
18	3808.0
19	3592.5
20	3377.0
21	3357.0
22	3337.0
23	3515.0
24	3693.0
25	4232.0
26	5664.5
27	6558.0
28	7248.5
29	7939.0
30	9381.0
31	10823.0
32	11749.5
33	12676.0
34	14642.0
35	16608.0
36	18146.5
37	19685.0
38	21512.5
39	23340.0
40	26525.5
41	29711.0
42	32510.0
43	35309.0
44	39464.5
45	43620.0
46	60631.5
47	77643.0
48	73072.5
49	68502.0
50	66105.0
51	63708.0
52	55433.0
53	47158.0
54	41375.5
55	35593.0
56	31891.5
57	28190.0
58	26255.5
59	24321.0
60	21573.5
61	18826.0
62	17039.0
63	15252.0
64	13277.0
65	11302.0
66	9176.5
67	7051.0
68	5656.0
69	4261.0
70	3482.5
71	2704.0
72	2309.0
73	1914.0
74	1514.5
75	923.0
76	731.0
77	641.0
78	551.0
79	400.0
80	249.0
81	189.5
82	130.0
83	75.5
84	21.0
85	13.0
86	5.0
87	3.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	680717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.50844474831694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.19724671251423	29.342374650027285
2	8.800510552643342	6.777879487468927
3	3.2295357877966473	3.7309320134123825
4	1.8496321346895248	2.849058274536123
5	1.3731440131113692	2.643882018019068
6	1.0165396148715085	2.3487215756252917
7	0.8656777300061356	2.333513212505279
8	0.7267415835671848	2.238855049368104
9	0.645022756284548	2.2354940854611556
>10	5.183583474570455	34.58206727160078
>50	0.07008170585367843	1.8577566154867047
>100	0.03327902220157786	2.202260249945318
>500	0.003915179082538571	1.0099911969866289
>1k	0.004306696990792429	3.2087837954122143
>5k	3.915179082538571E-4	0.9505888187790259
>10k+	3.915179082538571E-4	1.6878416853657725
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11195	1.6445894549423623	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTC	6305	0.9262292553293071	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCG	4195	0.6162619708336945	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGC	3837	0.5636703652178512	No Hit
GCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	2460	0.3613836587010461	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCT	1745	0.2563473513956607	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCC	1685	0.24753311581758647	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTT	1573	0.23107987607184777	No Hit
CTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGCT	1362	0.20008314762228652	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGCTAAGT	1193	0.17525638407737723	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCT	1156	0.1698209388042314	No Hit
CCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	1063	0.15615887365821626	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTC	1014	0.1489605812694556	No Hit
CGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTG	870	0.12780641588207728	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTA	811	0.11913908423030423	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	764	0.11223459969414602	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTT	750	0.11017794472592869	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4690392630123825E-4	0.0	0.0	0.2088973832003608	0.0
2	1.4690392630123825E-4	0.0	0.0	0.9147707490778106	0.0
3	1.4690392630123825E-4	0.0	0.0	1.2968678613873312	0.0
4	1.4690392630123825E-4	0.0	0.0	1.9809994461721978	0.0
5	1.4690392630123825E-4	0.0	0.0	4.001222240666826	0.0
6	1.4690392630123825E-4	0.0	0.0	5.180420057086866	0.0
7	1.4690392630123825E-4	0.0	0.0	6.155127608095581	0.0
8	1.4690392630123825E-4	0.0	0.0	7.530295262201473	0.0
9	1.4690392630123825E-4	0.0	0.0	8.106011749376025	0.0
10	1.4690392630123825E-4	0.0	0.0	9.811713237659703	0.0
11	1.4690392630123825E-4	0.0	0.0	11.303081897469873	0.0
12	1.4690392630123825E-4	0.0	0.0	13.112791365574827	0.0
13	1.4690392630123825E-4	0.0	0.0	13.713481520220592	0.0
14	1.4690392630123825E-4	0.0	0.0	13.99803442546609	0.0
15	1.4690392630123825E-4	0.0	0.0	14.461075601167593	0.0
16	1.4690392630123825E-4	0.0	0.0	15.11215380253468	0.0
17	1.4690392630123825E-4	0.0	0.0	15.944070737178592	0.0
18	1.4690392630123825E-4	0.0	0.0	16.813007461250418	0.0
19	1.4690392630123825E-4	0.0	0.0	17.536068586505113	0.0
20	1.4690392630123825E-4	0.0	0.0	18.105908916627616	0.0
21	1.4690392630123825E-4	0.0	0.0	18.67104832110848	0.0
22	1.4690392630123825E-4	0.0	0.0	19.30949278481366	0.0
23	1.4690392630123825E-4	0.0	0.0	19.915324576879968	0.0
24	1.4690392630123825E-4	0.0	0.0	20.415973157714586	0.0
25	1.4690392630123825E-4	0.0	0.0	20.8327395966312	0.0
26	1.4690392630123825E-4	0.0	0.0	21.294752444848594	0.0
27	1.4690392630123825E-4	0.0	0.0	21.754121022392564	0.0
28	1.4690392630123825E-4	0.0	0.0	22.202324901537644	0.0
29	2.938078526024765E-4	0.0	0.0	22.651263300314227	0.0
30	2.938078526024765E-4	0.0	0.0	23.1818802821143	0.0
31	2.938078526024765E-4	0.0	0.0	23.647712632415526	0.0
32	2.938078526024765E-4	0.0	0.0	24.07534996187843	0.0
33	2.938078526024765E-4	0.0	0.0	24.527960958812546	0.0
34	2.938078526024765E-4	0.0	0.0	25.009365125301702	0.0
35	2.938078526024765E-4	0.0	0.0	25.475344379529233	0.0
36	2.938078526024765E-4	0.0	0.0	25.914734023096234	0.0
37	2.938078526024765E-4	0.0	0.0	26.33884565832791	0.0
38	2.938078526024765E-4	0.0	0.0	26.767805123127527	0.0
39	2.938078526024765E-4	0.0	0.0	27.226732988892593	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	7.031258E-4	45.0	1
ACGTTAG	20	7.031258E-4	45.0	1
ATTCGGT	20	7.031258E-4	45.0	45
CGTAAGG	70	0.0	45.0	2
TTACCGG	40	6.8084773E-9	45.0	2
TAATGCG	40	6.8084773E-9	45.0	1
CGACGGT	50	2.1827873E-11	44.999996	28
ATTACCG	25	3.8891252E-5	44.999996	1
CGGTCTA	55	1.8189894E-12	44.999996	31
CACGTAT	25	3.8891252E-5	44.999996	45
CGTTTTT	5645	0.0	43.365807	1
ACGGTCT	60	3.6379788E-12	41.249996	30
GTACACG	50	1.0804797E-9	40.499996	1
TGAACCG	50	1.0804797E-9	40.499996	1
AACGGGC	40	3.4554432E-7	39.375	4
CGGATAG	80	0.0	39.375	2
TTACGAG	40	3.4554432E-7	39.375	1
GTACGGG	235	0.0	39.255318	3
CGTTATT	265	0.0	39.056602	1
TCGGCGT	105	0.0	38.571426	4
>>END_MODULE