FastQCFastQC Report
Sat 18 Jun 2016
SRR3551261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824934
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88661.074752646878417No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC68880.8349758889802094No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG46180.5598023599463714No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC44650.5412554216458529No Hit
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC31010.3759088605876349No Hit
GAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT21190.2568690343712346No Hit
CTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCT13530.16401312104968396No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCC12110.1467996227576994No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT12060.14619351366291122No Hit
CCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC12000.1454661827491654No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTC10490.12716168808656209No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTT9740.11807005166473925No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACATGAGT9550.11576683710454412No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA8740.10594786976897545No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTC207.032127E-445.023
ATATGCG253.8898455E-545.01
ACGATAG253.8898455E-545.01
GTTAACG207.032127E-445.01
AATTGCG302.1647538E-644.9999961
CGTTTTT43900.043.052391
TACGGGA1900.040.2631574
TATTACG451.9272193E-840.0000041
TCTAGCG403.4566438E-739.3751
TGCGTAG403.4566438E-739.3751
CGAACAC750.039.00000434
TGCAACG356.246146E-638.5714261
CGCGCAA659.094947E-1238.07692718
CCTCGAC301.13964285E-437.49999626
ATCGTCA301.13964285E-437.49999624
GGCGAAC1200.037.49999632
TATCGAG301.13964285E-437.4999961
CTAGCGA301.13964285E-437.49999617
TTACACG4100.037.31707434
CGTTATT1950.036.9230771