##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551261_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	824934
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.579428172435613	31.0	31.0	34.0	30.0	34.0
2	31.727792768876057	33.0	31.0	34.0	30.0	34.0
3	31.7020282834748	33.0	31.0	34.0	30.0	34.0
4	35.44061827976541	37.0	35.0	37.0	33.0	37.0
5	34.23111545893369	37.0	35.0	37.0	32.0	37.0
6	34.68848538186085	37.0	35.0	37.0	32.0	37.0
7	35.425980745126274	37.0	35.0	37.0	32.0	37.0
8	35.15030293332558	37.0	35.0	37.0	32.0	37.0
9	37.042617954890936	39.0	37.0	39.0	33.0	39.0
10	36.614244291058434	39.0	35.0	39.0	32.0	39.0
11	36.79706618953759	39.0	37.0	39.0	33.0	39.0
12	36.818792776149365	39.0	37.0	39.0	33.0	39.0
13	36.924164842278294	39.0	37.0	39.0	33.0	39.0
14	37.96345768267522	40.0	37.0	41.0	33.0	41.0
15	38.068558211929684	40.0	37.0	41.0	33.0	41.0
16	38.1266343731741	40.0	37.0	41.0	33.0	41.0
17	38.01620978163101	40.0	37.0	41.0	33.0	41.0
18	37.884262256132004	39.0	37.0	41.0	33.0	41.0
19	37.79127542324598	39.0	37.0	41.0	33.0	41.0
20	37.83843192303869	40.0	36.0	41.0	33.0	41.0
21	37.720662501484966	39.0	36.0	41.0	33.0	41.0
22	37.82551573822875	40.0	36.0	41.0	33.0	41.0
23	37.88525748726565	40.0	36.0	41.0	34.0	41.0
24	37.752537778779875	40.0	36.0	41.0	33.0	41.0
25	37.480183869254994	39.0	35.0	41.0	33.0	41.0
26	37.59339171376134	39.0	35.0	41.0	33.0	41.0
27	37.648221554694075	40.0	36.0	41.0	33.0	41.0
28	37.530863075106616	40.0	35.0	41.0	33.0	41.0
29	37.552148414297385	40.0	35.0	41.0	33.0	41.0
30	37.2574896900843	39.0	35.0	41.0	32.0	41.0
31	37.175228563739644	39.0	35.0	41.0	32.0	41.0
32	37.100912315409474	39.0	35.0	41.0	32.0	41.0
33	37.00608412309349	40.0	35.0	41.0	32.0	41.0
34	36.808387095210044	40.0	35.0	41.0	31.0	41.0
35	36.709970737052906	40.0	35.0	41.0	31.0	41.0
36	36.525011697905526	39.0	35.0	41.0	30.0	41.0
37	36.511807005166474	39.0	35.0	41.0	30.0	41.0
38	36.34662409356385	39.0	35.0	41.0	30.0	41.0
39	36.29586003243896	39.0	35.0	41.0	30.0	41.0
40	36.1562658346001	39.0	35.0	41.0	30.0	41.0
41	36.0092686202775	39.0	35.0	41.0	29.0	41.0
42	36.091121229092266	39.0	35.0	41.0	29.0	41.0
43	36.028338024617724	39.0	35.0	41.0	29.0	41.0
44	36.0913964026213	39.0	35.0	41.0	29.0	41.0
45	36.090187821086296	39.0	35.0	41.0	29.0	41.0
46	36.01458177260241	39.0	35.0	41.0	29.0	41.0
47	35.91316517442608	39.0	35.0	41.0	29.0	41.0
48	35.86932045472729	39.0	35.0	41.0	29.0	41.0
49	35.83636630324365	39.0	35.0	41.0	29.0	41.0
50	35.7127855076891	38.0	35.0	40.0	28.0	41.0
51	34.811266113410284	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	20.0
9	41.0
10	49.0
11	52.0
12	41.0
13	46.0
14	69.0
15	102.0
16	130.0
17	223.0
18	373.0
19	607.0
20	1176.0
21	1914.0
22	2722.0
23	3678.0
24	5048.0
25	7205.0
26	9841.0
27	11315.0
28	11818.0
29	12049.0
30	13375.0
31	16325.0
32	20729.0
33	29394.0
34	49806.0
35	60785.0
36	68545.0
37	101459.0
38	171316.0
39	224618.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.726285981666415	19.471375891889533	21.73616313547508	15.066174990968975
2	35.761527588873776	25.684600222563258	21.857506176251675	16.696366012311287
3	30.908412066904745	25.30238782738983	27.928294869650177	15.860905236055247
4	27.350066793222243	23.844695454424233	29.95827544991478	18.84696230243874
5	25.92874096594394	30.521229577154052	25.143950910012197	18.406078546889812
6	24.774588027648274	34.6342858943867	26.383322787035084	14.20780329092994
7	72.8538283062645	6.417361873798388	15.82344769399734	4.905362125939773
8	72.38712430327759	8.686391881047454	13.176326833419399	5.75015698225555
9	65.76586732999246	8.683240113754556	15.538333975808975	10.012558580444011
10	37.78253775453552	24.315642221074658	22.584230980902714	15.317589043487114
11	29.719468466568234	24.109928794303546	27.34291470590374	18.827688033224476
12	26.12584764356906	21.61288054559516	31.317172040429902	20.944099770405874
13	24.666094499681186	22.008427340853935	33.87664952590146	19.448828633563412
14	20.23422479858995	27.62390688224755	31.415604157423505	20.726264161739
15	19.526046932239428	25.029517512916183	35.571694220386114	19.872741334458272
16	21.479900210198636	24.82562241342944	32.17324052590874	21.52123685046319
17	21.764650262930125	25.425549195450785	31.057757347860555	21.75204319375853
18	22.588716188204145	24.731190616461436	30.817738146324437	21.86235504900998
19	22.89031607377075	26.21955210962332	28.99771375649446	21.892418060111478
20	24.916053890371835	25.78715388140142	30.51868391895594	18.778108309270802
21	23.860333069069767	27.137686166408464	29.83511408185382	19.166866682667948
22	22.028913828257775	24.211876344046917	30.720033360244575	23.03917646745073
23	22.409671561603716	26.03905282119539	30.475771395044937	21.075504222155956
24	21.87520456181949	24.865746835504414	30.48959068240611	22.769457920269986
25	21.671067018694828	27.27284849454623	28.895402541294214	22.160681945464727
26	21.00434701442782	25.94777279152029	30.603660414045248	22.444219780006645
27	23.15979217731358	26.352168779562973	29.207912390567003	21.280126652556447
28	20.388418952304065	26.85620910278883	30.352973692440848	22.402398252466256
29	23.277862228978318	25.799397285116143	29.42611166469075	21.496628821214788
30	22.112314439700633	27.49347220504913	29.274584390993702	21.119628964256535
31	23.362475058610748	26.128393301767172	26.7406847093222	23.768446930299877
32	23.9552497533136	28.755876227673973	26.858997204624856	20.429876814387576
33	22.92013664123433	27.18035624668155	27.820771116234756	22.078735995849367
34	23.93512693136663	26.164153738359673	27.893989094885168	22.006730235388527
35	22.9894755216781	28.429425869221053	26.689650323541038	21.891448285559814
36	23.477029677525717	28.89867553040607	26.400172619870194	21.22412217219802
37	22.621567301141667	29.24088472532348	27.279152029132025	20.858395944402826
38	21.46220182463082	28.209165824175024	26.96652095804028	23.362111393153874
39	24.546084898913126	25.94898500970987	27.651424235150934	21.853505856226075
40	23.63086016578296	25.246019681574527	30.118773138214692	21.00434701442782
41	22.250507313312337	27.093440202488928	27.44995357204334	23.206098912155397
42	22.49464805669302	27.60135962392143	28.487854785958632	21.416137533426916
43	23.9552497533136	25.28420455454618	28.209771933269813	22.550773758870406
44	22.616597206564403	25.287235100020123	28.508704938819346	23.587462754596125
45	22.19547260750557	24.457108083798218	28.123098332715102	25.224320975981108
46	23.747354333801248	26.600794730245088	27.578327478319476	22.073523457634188
47	21.199635364768575	26.260282640793083	30.975811398245195	21.56427059619315
48	21.707069898925248	25.179711346604698	28.896251094026915	24.21696766044314
49	21.525115948669832	24.681489670688805	31.145521944785887	22.647872435855472
50	21.23697168500753	23.93355104772018	30.41613026011778	24.413347007154513
51	21.258427946963028	24.142658685422106	28.366511745182038	26.23240162243283
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	475.0
1	872.5
2	1270.0
3	8053.5
4	14837.0
5	9864.5
6	4892.0
7	4611.0
8	4330.0
9	4209.5
10	4089.0
11	3984.5
12	3880.0
13	3846.0
14	3812.0
15	3606.0
16	3400.0
17	3266.5
18	3133.0
19	3198.0
20	3263.0
21	3492.0
22	3721.0
23	3887.0
24	4053.0
25	4416.0
26	6530.0
27	8281.0
28	8548.5
29	8816.0
30	10458.5
31	12101.0
32	14265.0
33	16429.0
34	18323.5
35	20218.0
36	21939.0
37	23660.0
38	25177.5
39	26695.0
40	30727.5
41	34760.0
42	36922.0
43	39084.0
44	44134.0
45	49184.0
46	66271.0
47	83358.0
48	78883.5
49	74409.0
50	73402.5
51	72396.0
52	64642.5
53	56889.0
54	51746.0
55	46603.0
56	42813.5
57	39024.0
58	37191.0
59	35358.0
60	33864.0
61	32370.0
62	30423.5
63	28477.0
64	24330.5
65	20184.0
66	16570.5
67	12957.0
68	10904.5
69	8852.0
70	7315.5
71	5779.0
72	4888.5
73	3998.0
74	3304.0
75	1892.5
76	1175.0
77	931.5
78	688.0
79	535.5
80	383.0
81	262.0
82	141.0
83	113.5
84	86.0
85	57.0
86	28.0
87	16.5
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	824934.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.84328892679266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.01076584370226	24.307569662979105
2	8.764458891079926	5.757073112934696
3	3.2000334440936835	3.1529886893930477
4	1.8891753265622195	2.481869243346034
5	1.2917145331917386	2.1212076812276686
6	1.0678569061574	2.104315974083904
7	0.9199137180526546	2.114909442080639
8	0.8150917478121985	2.1416235020192365
9	0.7064518171707421	2.088198102977675
>10	7.199030537413731	43.61351530645682
>50	0.08426500413266341	1.8830490791004544
>100	0.041752928662121054	2.5796712106374358
>500	0.005314009102451759	1.1566321699267432
>1k	0.003416148708718988	2.533420883514904
>5k	7.591441574931083E-4	1.9639559393216117
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8866	1.074752646878417	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC	6888	0.8349758889802094	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	4618	0.5598023599463714	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC	4465	0.5412554216458529	No Hit
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	3101	0.3759088605876349	No Hit
GAACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCT	2119	0.2568690343712346	No Hit
CTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCT	1353	0.16401312104968396	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCC	1211	0.1467996227576994	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT	1206	0.14619351366291122	No Hit
CCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	1200	0.1454661827491654	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTC	1049	0.12716168808656209	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTT	974	0.11807005166473925	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACATGAGT	955	0.11576683710454412	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	874	0.10594786976897545	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4244363791527565E-4	0.0	0.0	0.16934688108382004	0.0
2	2.4244363791527565E-4	0.0	0.0	0.8314604562304378	0.0
3	2.4244363791527565E-4	0.0	0.0	1.1146346253154797	0.0
4	2.4244363791527565E-4	0.0	0.0	1.7276533637842542	0.0
5	2.4244363791527565E-4	0.0	0.0	3.469853345843425	0.0
6	2.4244363791527565E-4	0.0	0.0	4.398170035421016	0.0
7	2.4244363791527565E-4	0.0	0.0	5.231206375297902	0.0
8	2.4244363791527565E-4	0.0	0.0	6.459062179519816	0.0
9	2.4244363791527565E-4	0.0	0.0	6.999832713889838	0.0
10	2.4244363791527565E-4	0.0	0.0	8.5197118799807	0.0
11	2.4244363791527565E-4	0.0	0.0	9.689017545646076	0.0
12	2.4244363791527565E-4	0.0	0.0	11.127556871216363	0.0
13	2.4244363791527565E-4	0.0	0.0	11.582017470488548	0.0
14	2.4244363791527565E-4	0.0	0.0	11.790397777276727	0.0
15	2.4244363791527565E-4	0.0	0.0	12.211886041792434	0.0
16	2.4244363791527565E-4	0.0	0.0	12.80017552919385	0.0
17	2.4244363791527565E-4	0.0	0.0	13.457803897039035	0.0
18	2.4244363791527565E-4	0.0	0.0	14.154950577864412	0.0
19	2.4244363791527565E-4	0.0	0.0	14.706509854121663	0.0
20	2.4244363791527565E-4	0.0	0.0	15.15515180608388	0.0
21	2.4244363791527565E-4	0.0	0.0	15.681254500360028	0.0
22	2.4244363791527565E-4	0.0	0.0	16.225904132936694	0.0
23	2.4244363791527565E-4	0.0	0.0	16.715882725163468	0.0
24	2.4244363791527565E-4	0.0	0.0	17.14221986243748	0.0
25	2.4244363791527565E-4	0.0	0.0	17.52552325398153	0.0
26	2.4244363791527565E-4	0.0	0.0	17.87767263805347	0.0
27	2.4244363791527565E-4	0.0	0.0	18.24655063314156	0.0
28	2.4244363791527565E-4	0.0	0.0	18.611185864566135	0.0
29	2.4244363791527565E-4	0.0	0.0	18.988913052438136	0.0
30	2.4244363791527565E-4	0.0	0.0	19.428220924340614	0.0
31	2.4244363791527565E-4	0.0	0.0	19.84292076699469	0.0
32	2.4244363791527565E-4	0.0	0.0	20.25822671874356	0.0
33	2.4244363791527565E-4	0.0	0.0	20.672562895940768	0.0
34	2.4244363791527565E-4	0.0	0.0	21.081201647646964	0.0
35	2.4244363791527565E-4	0.0	0.0	21.4888706248015	0.0
36	2.4244363791527565E-4	0.0	0.0	21.893630278301053	0.0
37	2.4244363791527565E-4	0.0	0.0	22.29705649179207	0.0
38	2.4244363791527565E-4	0.0	0.0	22.702179810748497	0.0
39	2.4244363791527565E-4	0.0	0.0	23.11857675886798	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGTC	20	7.032127E-4	45.0	23
ATATGCG	25	3.8898455E-5	45.0	1
ACGATAG	25	3.8898455E-5	45.0	1
GTTAACG	20	7.032127E-4	45.0	1
AATTGCG	30	2.1647538E-6	44.999996	1
CGTTTTT	4390	0.0	43.05239	1
TACGGGA	190	0.0	40.263157	4
TATTACG	45	1.9272193E-8	40.000004	1
TCTAGCG	40	3.4566438E-7	39.375	1
TGCGTAG	40	3.4566438E-7	39.375	1
CGAACAC	75	0.0	39.000004	34
TGCAACG	35	6.246146E-6	38.571426	1
CGCGCAA	65	9.094947E-12	38.076927	18
CCTCGAC	30	1.13964285E-4	37.499996	26
ATCGTCA	30	1.13964285E-4	37.499996	24
GGCGAAC	120	0.0	37.499996	32
TATCGAG	30	1.13964285E-4	37.499996	1
CTAGCGA	30	1.13964285E-4	37.499996	17
TTACACG	410	0.0	37.317074	34
CGTTATT	195	0.0	36.923077	1
>>END_MODULE