SRR3551259_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551259_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	677288
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7743	1.1432359646118047	No Hit
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	4165	0.6149525755660812	TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	4096	0.604764885838816	No Hit
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	2762	0.4078028844450219	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG	2558	0.3776827582948465	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC	2446	0.3611462184476914	No Hit
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	2366	0.349334404271152	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCC	1847	0.2727052598008528	No Hit
ACAGCTGGGAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATC	1757	0.259416968852246	No Hit
ACAGCTGGGAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATG	1463	0.21600855175346378	No Hit
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1393	0.20567321434899186	TruSeq Adapter, Index 16 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	1338	0.19755259210262102	No Hit
GAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT	1204	0.17776780335691758	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTT	997	0.14720473417512195	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	985	0.14543296204864106	No Hit
CGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	968	0.1429229515361264	No Hit
ACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	966	0.14262765618171294	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCT	751	0.11088340558226338	No Hit
CGTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT	743	0.10970222416460944	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCT	734	0.10837339506974877	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTT	712	0.10512514617120043	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAG	30	2.1641754E-6	45.000004	1
TCGTTCA	25	3.8891034E-5	45.0	16
TCGTAAA	20	7.031232E-4	45.0	13
TAGTGCG	45	3.8380676E-10	45.0	1
TAGTCCG	20	7.031232E-4	45.0	1
GCGTAAG	20	7.031232E-4	45.0	1
TCGAAAG	20	7.031232E-4	45.0	1
CGTTTTT	3625	0.0	43.199997	1
GCGAGAC	170	0.0	42.35294	21
CGGGTAT	60	3.6379788E-12	41.250004	6
ACGGGCG	60	3.6379788E-12	41.250004	5
AGTACGG	55	6.002665E-11	40.909092	2
ACACGTG	160	0.0	40.78125	42
TTGTCGG	50	1.0804797E-9	40.5	2
GCAAACG	50	1.0804797E-9	40.5	1
CACGACC	180	0.0	40.0	27
CGACAGG	90	0.0	40.0	2
TCGATAG	45	1.9263098E-8	40.0	1
GCGATAT	45	1.9263098E-8	40.0	9
TTTTGCG	90	0.0	40.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content