##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551259_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	677288
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.501940090478495	31.0	31.0	34.0	30.0	34.0
2	31.640500053153165	31.0	31.0	34.0	30.0	34.0
3	31.61354401672553	31.0	31.0	34.0	30.0	34.0
4	35.37819952516507	37.0	35.0	37.0	33.0	37.0
5	34.214251544394706	37.0	35.0	37.0	32.0	37.0
6	34.71201320560825	37.0	35.0	37.0	32.0	37.0
7	35.41447951240831	37.0	35.0	37.0	33.0	37.0
8	35.21871198072312	37.0	35.0	37.0	32.0	37.0
9	37.012430457944035	39.0	37.0	39.0	33.0	39.0
10	36.59451075465681	38.0	35.0	39.0	32.0	39.0
11	36.80731387533811	39.0	37.0	39.0	33.0	39.0
12	36.86837357224696	39.0	37.0	39.0	33.0	39.0
13	37.00522820424989	39.0	37.0	39.0	33.0	39.0
14	38.01531106412634	40.0	37.0	41.0	33.0	41.0
15	38.12251213663907	40.0	37.0	41.0	33.0	41.0
16	38.13839902670651	40.0	37.0	41.0	33.0	41.0
17	38.05589941059047	40.0	37.0	41.0	33.0	41.0
18	37.93992068366781	39.0	37.0	41.0	33.0	41.0
19	37.852473393888566	39.0	37.0	41.0	33.0	41.0
20	37.934943480469165	40.0	36.0	41.0	34.0	41.0
21	37.7866151474705	39.0	36.0	41.0	33.0	41.0
22	37.93203482122819	40.0	36.0	41.0	34.0	41.0
23	37.986800297657716	40.0	36.0	41.0	34.0	41.0
24	37.85503360461133	40.0	36.0	41.0	33.0	41.0
25	37.50493143241871	39.0	36.0	41.0	33.0	41.0
26	37.67195048487497	39.0	36.0	41.0	33.0	41.0
27	37.742362185658095	40.0	36.0	41.0	33.0	41.0
28	37.54045103412433	40.0	36.0	41.0	33.0	41.0
29	37.56084856073044	40.0	36.0	41.0	33.0	41.0
30	37.25763043195804	39.0	36.0	41.0	32.0	41.0
31	37.17745479028124	39.0	35.0	41.0	32.0	41.0
32	37.198447337026494	39.0	36.0	41.0	32.0	41.0
33	37.122302476937435	40.0	36.0	41.0	32.0	41.0
34	36.936759546898806	40.0	36.0	41.0	31.0	41.0
35	36.9207545386896	40.0	36.0	41.0	31.0	41.0
36	36.74682705141683	40.0	35.0	41.0	31.0	41.0
37	36.777983664260994	40.0	35.0	41.0	31.0	41.0
38	36.5774323478343	39.0	35.0	41.0	30.0	41.0
39	36.51503348649319	39.0	35.0	41.0	30.0	41.0
40	36.430007618620145	39.0	35.0	41.0	30.0	41.0
41	36.253968769563315	39.0	35.0	41.0	30.0	41.0
42	36.403603784505265	39.0	35.0	41.0	30.0	41.0
43	36.3515254958009	39.0	35.0	41.0	30.0	41.0
44	36.40529287393251	39.0	35.0	41.0	30.0	41.0
45	36.40584802919881	39.0	35.0	41.0	30.0	41.0
46	36.30128689715453	39.0	35.0	41.0	30.0	41.0
47	36.247174023458264	39.0	35.0	41.0	30.0	41.0
48	36.20029883889867	39.0	35.0	41.0	30.0	41.0
49	36.18343156825457	39.0	35.0	41.0	30.0	41.0
50	36.094231700546885	39.0	35.0	41.0	29.0	41.0
51	35.117161384817095	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	27.0
10	39.0
11	48.0
12	28.0
13	35.0
14	44.0
15	47.0
16	68.0
17	122.0
18	230.0
19	447.0
20	892.0
21	1454.0
22	2041.0
23	2960.0
24	4131.0
25	5978.0
26	7691.0
27	8923.0
28	8678.0
29	9124.0
30	10231.0
31	12825.0
32	16529.0
33	23111.0
34	37441.0
35	48400.0
36	56411.0
37	83865.0
38	144742.0
39	190676.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.36421729013359	21.801803664024757	22.336140607835958	15.497838438005692
2	34.14647830760327	24.40704692833772	22.599987007004405	18.846487757054607
3	29.297728588133854	26.172617852375947	28.749217467310807	15.780436092179398
4	28.276892547926437	23.988613411133816	29.965539032139947	17.768955008799804
5	23.25421386470748	29.85982329525992	26.751692042380785	20.134270797651812
6	22.749554104014834	32.654498529429134	29.461765157510545	15.134182209045488
7	72.30793990148948	6.078802518278782	15.845991660859191	5.767265919372557
8	71.56276207462705	6.910797179338775	14.447620510034136	7.0788202360000465
9	65.42194753192143	8.03720130874901	17.142338266734388	9.398512892595173
10	33.23061976588984	28.989735533480587	23.54434155041873	14.23530315021084
11	24.830795761921074	25.367790363921994	30.36226834079446	19.43914553336247
12	22.085139556584497	20.448169759393345	36.29622848773343	21.170462196288728
13	23.166658792123883	22.96098557777489	35.412409491973875	18.459946138127357
14	18.27435300787848	28.346286956213607	32.415899883063034	20.963460152844878
15	17.468787281038495	24.94507506407909	38.29685451388479	19.289283140997625
16	18.50453573664379	27.17987030628034	32.92823732297043	21.38735663410543
17	18.613204427067952	26.405162943976567	30.840646814944307	24.140985814011174
18	20.22138292720379	24.939316804668028	33.24878043018627	21.59051983794191
19	20.73165329963029	26.39128406231913	30.438306894555932	22.438755743494642
20	20.956225416661745	26.188563801514274	31.9748762712465	20.88033451057748
21	21.359894166144976	27.545445955044233	32.04190831669836	19.052751562112423
22	19.109891213191435	26.02201722162507	30.104770791745906	24.76332077343759
23	18.42022891295874	26.979364760633583	31.718707551292802	22.88169877511487
24	19.719085529346454	24.951866857230602	31.37070197611651	23.958345637306433
25	18.367371044518727	29.042150458888983	29.045103412433114	23.545375084159176
26	18.09451813704067	27.34818865829603	30.634973600595316	23.922319604067987
27	20.382318895359138	26.040473181275914	29.187878716292037	24.389329207072915
28	17.657776307863124	25.6942393782261	31.65270903958139	24.995275274329384
29	18.43558427138824	25.954099290109966	29.691652590921443	25.918663847580348
30	19.40828716882626	25.312570132646673	29.70213557600312	25.57700712252395
31	18.950727017162567	26.508516318021286	26.605225546591697	27.93553111822445
32	18.10706818960324	27.364134607434355	27.45198497537237	27.076812227590036
33	17.78165270903958	25.917777961517107	28.82496072571786	27.475608603725448
34	17.134808235196843	25.391856935306695	29.217703547087797	28.255631282408665
35	17.18751845595965	24.997489989487484	29.43179267903757	28.38319887551529
36	17.62854206777619	27.868499072772586	29.086592409728212	25.416366449723014
37	17.663829862628603	26.229166912746127	29.359150021851853	26.747853202773413
38	17.516182185421858	26.087572790304865	29.569695609548667	26.82654941472461
39	19.54382773650205	24.95541040148356	29.49055645456586	26.010205407448527
40	20.239691239177425	23.42474693188127	32.51275675931066	23.822805069630647
41	17.1667001334735	26.72895430009095	29.2024958363355	26.901849730100047
42	19.160091423441727	26.41402180460897	30.08675777512668	24.339128996822623
43	20.482571668182516	25.083568585299016	29.453496887586965	24.980362858931503
44	19.6653417748432	24.585848265435086	28.34333400266947	27.405475957052243
45	19.411092474693188	22.582859876448424	28.94529358264136	29.060754066217033
46	21.691658496828527	24.293801159920154	29.0403786867625	24.974161656488818
47	17.66707811152715	24.076168483717414	32.340304272333185	25.916449132422247
48	18.240984632829758	23.329661827760127	31.324192957796388	27.10516058161373
49	19.76899044424233	22.66140844072241	32.431550536846956	25.138050578188302
50	18.80514640743672	21.801803664024757	32.59603004925526	26.79701987928326
51	18.8879767543497	21.18847521290795	29.221837682049586	30.701710350692764
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	262.0
1	529.5
2	797.0
3	6132.0
4	11467.0
5	7350.0
6	3233.0
7	3261.5
8	3290.0
9	3229.0
10	3168.0
11	3274.5
12	3381.0
13	3409.5
14	3438.0
15	3271.5
16	3105.0
17	2987.0
18	2869.0
19	2904.5
20	2940.0
21	2934.0
22	2928.0
23	2940.0
24	2952.0
25	3354.0
26	4664.5
27	5573.0
28	6354.5
29	7136.0
30	8024.5
31	8913.0
32	10306.5
33	11700.0
34	13792.0
35	15884.0
36	17076.5
37	18269.0
38	19693.0
39	21117.0
40	23941.5
41	26766.0
42	31009.5
43	35253.0
44	40738.0
45	46223.0
46	63311.5
47	80400.0
48	82508.5
49	84617.0
50	80863.0
51	77109.0
52	64150.0
53	51191.0
54	43240.0
55	35289.0
56	31110.0
57	26931.0
58	23887.5
59	20844.0
60	18757.5
61	16671.0
62	15094.5
63	13518.0
64	11256.5
65	8995.0
66	7582.0
67	6169.0
68	4906.5
69	3644.0
70	3097.5
71	2551.0
72	2390.0
73	2229.0
74	1715.0
75	1043.5
76	886.0
77	627.0
78	368.0
79	255.5
80	143.0
81	103.5
82	64.0
83	40.0
84	16.0
85	10.0
86	4.0
87	5.0
88	6.0
89	14.0
90	22.0
91	11.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	677288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.963139124735804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48653144202724	28.27742556052688
2	10.036947248555188	7.6206804956906975
3	3.673290286250993	4.183488905474611
4	1.9837677841445185	3.0124020952258896
5	1.360056981895786	2.581601621063901
6	1.0224374825648763	2.328896183817302
7	0.8319069547869975	2.2107259624389863
8	0.7185785473041263	2.1823597890685678
9	0.6296121434863742	2.151184805801671
>10	5.126251486083038	33.29735302261678
>50	0.07566381762325965	1.9736659165748702
>100	0.04340713735697655	3.094067143708296
>500	0.006371689887262612	1.7739560179943876
>1k	0.0047787674154469596	4.14159920853556
>5k	3.9823061795391326E-4	1.1705932714616112
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7743	1.1432359646118047	No Hit
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	4165	0.6149525755660812	TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	4096	0.604764885838816	No Hit
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	2762	0.4078028844450219	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG	2558	0.3776827582948465	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC	2446	0.3611462184476914	No Hit
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	2366	0.349334404271152	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCC	1847	0.2727052598008528	No Hit
ACAGCTGGGAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATC	1757	0.259416968852246	No Hit
ACAGCTGGGAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATG	1463	0.21600855175346378	No Hit
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	1393	0.20567321434899186	TruSeq Adapter, Index 16 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	1338	0.19755259210262102	No Hit
GAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT	1204	0.17776780335691758	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTT	997	0.14720473417512195	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC	985	0.14543296204864106	No Hit
CGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	968	0.1429229515361264	No Hit
ACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	966	0.14262765618171294	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCT	751	0.11088340558226338	No Hit
CGTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT	743	0.10970222416460944	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCT	734	0.10837339506974877	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTT	712	0.10512514617120043	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4764767720674217E-4	0.0	0.0	0.419762346298768	0.0
2	1.4764767720674217E-4	0.0	0.0	1.7806309871133108	0.0
3	1.4764767720674217E-4	0.0	0.0	2.5176881917293676	0.0
4	1.4764767720674217E-4	0.0	0.0	3.4620427351436907	0.0
5	1.4764767720674217E-4	0.0	0.0	5.995824523688594	0.0
6	1.4764767720674217E-4	0.0	0.0	7.6168483717414155	0.0
7	1.4764767720674217E-4	0.0	0.0	9.067634447974864	0.0
8	1.4764767720674217E-4	0.0	0.0	11.270833087253871	0.0
9	1.4764767720674217E-4	0.0	0.0	12.11936428816102	0.0
10	1.4764767720674217E-4	0.0	0.0	14.097400219699743	0.0
11	1.4764767720674217E-4	0.0	0.0	16.225593838957725	0.0
12	1.4764767720674217E-4	0.0	0.0	18.30890256434486	0.0
13	2.9529535441348434E-4	0.0	0.0	19.34657043975384	0.0
14	2.9529535441348434E-4	0.0	0.0	19.71244138387215	0.0
15	2.9529535441348434E-4	0.0	0.0	20.259328380245922	0.0
16	2.9529535441348434E-4	0.0	0.0	21.186408145427055	0.0
17	2.9529535441348434E-4	0.0	0.0	22.254048499309008	0.0
18	2.9529535441348434E-4	0.0	0.0	23.723142887516094	0.0
19	2.9529535441348434E-4	0.0	0.0	24.526493899197977	0.0
20	4.4294303162022657E-4	0.0	0.0	25.29278534390097	0.0
21	4.4294303162022657E-4	0.0	0.0	26.097317537000507	0.0
22	4.4294303162022657E-4	0.0	0.0	26.970801195355595	0.0
23	4.4294303162022657E-4	0.0	0.0	27.795265824878044	0.0
24	4.4294303162022657E-4	0.0	0.0	28.461747439789278	0.0
25	5.905907088269687E-4	0.0	0.0	29.03092923542127	0.0
26	5.905907088269687E-4	0.0	0.0	29.58918510293996	0.0
27	5.905907088269687E-4	0.0	0.0	30.181399936216202	0.0
28	5.905907088269687E-4	0.0	0.0	30.72636751278629	0.0
29	5.905907088269687E-4	0.0	0.0	31.283589846564535	0.0
30	5.905907088269687E-4	0.0	0.0	31.91817956319911	0.0
31	5.905907088269687E-4	0.0	0.0	32.47038187595233	0.0
32	5.905907088269687E-4	0.0	0.0	33.01357768039593	0.0
33	5.905907088269687E-4	0.0	0.0	33.532411618100426	0.0
34	5.905907088269687E-4	0.0	0.0	34.07206387829107	0.0
35	5.905907088269687E-4	0.0	0.0	34.59813255217869	0.0
36	5.905907088269687E-4	0.0	0.0	35.11061763976329	0.0
37	7.38238386033711E-4	0.0	0.0	35.5904725906852	0.0
38	7.38238386033711E-4	0.0	0.0	36.080958174366	0.0
39	7.38238386033711E-4	0.0	0.0	36.59004736537485	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAG	30	2.1641754E-6	45.000004	1
TCGTTCA	25	3.8891034E-5	45.0	16
TCGTAAA	20	7.031232E-4	45.0	13
TAGTGCG	45	3.8380676E-10	45.0	1
TAGTCCG	20	7.031232E-4	45.0	1
GCGTAAG	20	7.031232E-4	45.0	1
TCGAAAG	20	7.031232E-4	45.0	1
CGTTTTT	3625	0.0	43.199997	1
GCGAGAC	170	0.0	42.35294	21
CGGGTAT	60	3.6379788E-12	41.250004	6
ACGGGCG	60	3.6379788E-12	41.250004	5
AGTACGG	55	6.002665E-11	40.909092	2
ACACGTG	160	0.0	40.78125	42
TTGTCGG	50	1.0804797E-9	40.5	2
GCAAACG	50	1.0804797E-9	40.5	1
CACGACC	180	0.0	40.0	27
CGACAGG	90	0.0	40.0	2
TCGATAG	45	1.9263098E-8	40.0	1
GCGATAT	45	1.9263098E-8	40.0	9
TTTTGCG	90	0.0	40.0	1
>>END_MODULE