SRR3551257_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551257_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	625215
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCG	12955	2.072087202002511	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGC	12899	2.063130283182585	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTC	12466	1.9938741073070865	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8690	1.3899218668777942	No Hit
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	4726	0.755899970410179	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTACAGGT	3356	0.5367753492798477	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	3015	0.48223411146565587	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	2756	0.44080836192349826	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTA	2513	0.40194173204417677	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTC	2308	0.36915301136409073	No Hit
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	1247	0.19945138872227955	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	1038	0.1660228881264845	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGGTCGAATGC	1033	0.16522316323184824	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACAGGTCGAATGC	847	0.13547339715137993	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTT	818	0.13083499276248972	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC	797	0.12747614820501746	No Hit
TCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	723	0.11564021976440104	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATG	722	0.11548027478547379	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATC	20	7.030815E-4	45.000004	19
TCGCACC	20	7.030815E-4	45.000004	32
CGCATCC	20	7.030815E-4	45.000004	20
GTTACGT	20	7.030815E-4	45.000004	7
GTTCGAC	20	7.030815E-4	45.000004	9
CGCTATA	20	7.030815E-4	45.000004	43
CGGTCGA	195	0.0	45.000004	41
GTACGAG	20	7.030815E-4	45.000004	1
CCGATCG	45	3.8380676E-10	45.0	40
CCGTCGA	25	3.888757E-5	45.0	41
TCGGCCA	25	3.888757E-5	45.0	29
CGTAAGC	55	1.8189894E-12	45.0	43
CGGTCTA	50	2.1827873E-11	45.0	31
CCGCGTA	45	3.8380676E-10	45.0	40
CGATCGA	45	3.8380676E-10	45.0	41
TACCGCC	25	3.888757E-5	45.0	45
GTCGAAT	365	0.0	44.383564	43
GGTCGAA	355	0.0	43.09859	42
TAATACG	110	0.0	42.954544	4
CGATGAA	2050	0.0	42.914635	19

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content