##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551257_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	625215
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5462952744256	31.0	31.0	34.0	30.0	34.0
2	31.69587741816815	31.0	31.0	34.0	30.0	34.0
3	31.686786145565925	31.0	31.0	34.0	30.0	34.0
4	35.47220556128692	37.0	35.0	37.0	33.0	37.0
5	34.28981230456723	37.0	35.0	37.0	32.0	37.0
6	34.71901665826955	37.0	35.0	37.0	32.0	37.0
7	35.41423030477516	37.0	35.0	37.0	33.0	37.0
8	34.938742672520654	37.0	35.0	37.0	32.0	37.0
9	36.72575354078197	39.0	37.0	39.0	32.0	39.0
10	36.50015914525403	38.0	35.0	39.0	32.0	39.0
11	36.70228001567461	39.0	35.0	39.0	32.0	39.0
12	36.690208968114966	39.0	35.0	39.0	32.0	39.0
13	36.682880289180524	39.0	35.0	39.0	32.0	39.0
14	37.73396511600009	40.0	37.0	41.0	33.0	41.0
15	37.77807954063802	40.0	37.0	41.0	33.0	41.0
16	37.96752956982798	40.0	37.0	41.0	33.0	41.0
17	37.72582071767312	40.0	36.0	41.0	32.0	41.0
18	37.628830082451636	39.0	36.0	41.0	33.0	41.0
19	37.53236406676103	39.0	36.0	41.0	33.0	41.0
20	37.26094863367002	39.0	35.0	41.0	32.0	41.0
21	37.39130699039531	39.0	35.0	41.0	33.0	41.0
22	37.44913349807666	39.0	35.0	41.0	33.0	41.0
23	37.35977703669938	39.0	35.0	41.0	32.0	41.0
24	37.299621730124834	39.0	35.0	41.0	32.0	41.0
25	37.22487304367298	39.0	35.0	41.0	33.0	41.0
26	37.22916596690738	39.0	35.0	41.0	32.0	41.0
27	37.14859208432299	39.0	35.0	41.0	32.0	41.0
28	37.0217093319898	39.0	35.0	41.0	31.0	41.0
29	37.101667426405314	39.0	35.0	41.0	32.0	41.0
30	36.8274929424278	39.0	35.0	41.0	31.0	41.0
31	36.704731972201564	39.0	35.0	41.0	31.0	41.0
32	36.38528826083827	39.0	35.0	41.0	30.0	41.0
33	36.186956486968484	39.0	35.0	41.0	29.0	41.0
34	35.86640595635102	39.0	35.0	41.0	27.0	41.0
35	35.80425293698968	39.0	35.0	41.0	26.0	41.0
36	35.55037067248866	39.0	35.0	41.0	25.0	41.0
37	35.53321177514935	39.0	35.0	41.0	24.0	41.0
38	35.44003742712507	39.0	35.0	41.0	24.0	41.0
39	35.38089617171693	39.0	35.0	41.0	24.0	41.0
40	35.419340546851885	39.0	35.0	40.0	25.0	41.0
41	35.0941852002911	38.0	35.0	40.0	23.0	41.0
42	35.10267188087298	39.0	35.0	40.0	23.0	41.0
43	34.98875266908183	39.0	35.0	40.0	22.0	41.0
44	35.16724166886591	39.0	35.0	40.0	23.0	41.0
45	35.212675639579984	39.0	35.0	40.0	23.0	41.0
46	35.19295282422846	39.0	35.0	40.0	23.0	41.0
47	34.988255240197375	38.0	35.0	40.0	23.0	41.0
48	35.09689466823413	38.0	35.0	40.0	23.0	41.0
49	35.126794782594786	38.0	35.0	40.0	24.0	41.0
50	34.913911214542196	38.0	35.0	40.0	23.0	41.0
51	34.121273481922216	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	22.0
10	25.0
11	25.0
12	31.0
13	28.0
14	35.0
15	54.0
16	66.0
17	147.0
18	264.0
19	508.0
20	1294.0
21	2675.0
22	4600.0
23	6174.0
24	7192.0
25	8674.0
26	10263.0
27	10665.0
28	10036.0
29	9747.0
30	10635.0
31	12830.0
32	17112.0
33	23507.0
34	38429.0
35	47665.0
36	49415.0
37	71559.0
38	122299.0
39	159202.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.02044096830691	17.349871644154412	19.423238405988343	15.206448981550347
2	32.20316211223339	33.15291539710339	19.852210839471223	14.79171165119199
3	26.00289500411858	32.720744064041966	27.547643610597955	13.728717321241493
4	24.350023591884394	20.607151140007836	39.10638740273346	15.936437865374312
5	30.868901098022278	26.07535007957263	25.813200259110864	17.24254856329423
6	21.774109706261047	38.17822669001863	27.231912222195565	12.815751381524757
7	65.80952152459554	4.942299848851995	24.649280647457275	4.598897979095192
8	64.09171245091689	15.078173108450693	15.155906368209335	5.674208072423086
9	58.834480938557135	7.120590516862199	16.487288372799757	17.557640171780907
10	39.072958902137664	23.19266172436682	23.294386730964547	14.439992642530969
11	31.43478643346689	21.01117215677807	29.482498020680886	18.071543389074158
12	24.166726646033766	18.132802316003293	33.04511248130643	24.65535855665651
13	25.12799596938653	21.501723407147942	36.99815263549339	16.37212798797214
14	18.303943443455452	30.78189102948586	30.57956063114289	20.334604895915806
15	16.40795566325184	22.29473061266924	39.91730844589461	21.380005278184306
16	18.266996153323255	23.355165822956902	31.644794190798365	26.733043832921478
17	18.272114392648927	26.870756459777834	33.25064177922795	21.606487368345288
18	18.72363906816055	23.297425685564164	33.29846532792719	24.680469918348088
19	19.47777964380253	24.220788048911174	30.795166462736816	25.506265844549475
20	23.55077853218493	24.414961253328855	35.35103924250058	16.683220971985637
21	20.015834552913798	30.346840686803738	31.86535831673904	17.771966443543423
22	18.73019681229657	21.523475924282046	35.70691682061371	24.039410442807675
23	21.50076373727438	26.257527410570763	32.23355165822957	20.00815719392529
24	20.518381676703214	25.1595051302352	31.12225394464304	23.199859248418544
25	17.220316211223338	32.41141047479667	29.677790839951058	20.690482474028933
26	17.1872076005854	23.55525699159489	35.960749502171254	23.296785905648456
27	23.000247914717338	24.488216053677537	30.44472701390721	22.06680901769791
28	15.495469558471886	27.165055221003975	36.15044424717897	21.18903097334517
29	19.111185752101278	22.21059955375351	36.27552122070008	22.402693473445133
30	17.498300584598898	27.995809441552105	31.895268027798434	22.610621946050557
31	20.42081523955759	23.76110617947426	30.411458458290348	25.406620122677797
32	24.267172092800077	28.054349303839476	28.01020448965556	19.668274113704886
33	21.699575346080948	23.580368353286467	26.9233783578449	27.796677942787678
34	19.909631086906103	26.58909335188695	30.76893548619275	22.732340075014196
35	23.677934790432094	23.466167638332415	27.510536375486833	25.345361195748662
36	21.542029541837607	26.638196460417618	31.05667650328287	20.763097494461906
37	19.182041377766048	27.201042841262606	30.088689490815156	23.528226290156187
38	19.5539134537719	25.166542709307997	27.778124325232117	27.50141951168798
39	21.42159097270539	30.067256863638907	27.862575274105705	20.648576889549993
40	21.829130779012022	23.12932351271163	29.029853730316773	26.011691977959583
41	21.184072678998426	26.925297697592026	26.9304159369177	24.960213686491848
42	23.674895835832473	23.264317074926225	31.470294218788737	21.590492870452564
43	25.689402845421178	22.669001863359004	27.83986308709804	23.80173220412178
44	20.460001759394768	23.72271938453172	31.156482170133476	24.66079668594004
45	19.91107059171645	22.057372263941204	30.112361347696392	27.91919579664595
46	25.59903393232728	26.382764329070802	27.499500171940856	20.51870156666107
47	17.985812880369153	24.79547035819678	35.36735363035116	21.851363131082906
48	20.433930727829626	25.42741296993834	29.10070935598154	25.03794694625049
49	21.292835264668955	21.287077245427575	35.10744303959438	22.312644450309094
50	22.400934078676933	21.19606855241797	31.60784690066617	24.795150468238923
51	20.118839119342947	21.5912925953472	29.49977207840503	28.790096206904824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	262.0
1	515.5
2	769.0
3	7364.5
4	13960.0
5	9233.0
6	4506.0
7	4260.0
8	4014.0
9	3947.5
10	3881.0
11	3722.0
12	3563.0
13	3526.0
14	3489.0
15	3265.0
16	3041.0
17	2909.0
18	2777.0
19	2686.0
20	2595.0
21	2616.5
22	2638.0
23	2877.0
24	3116.0
25	3561.0
26	4746.5
27	5487.0
28	6279.0
29	7071.0
30	7775.5
31	8480.0
32	9629.0
33	10778.0
34	13042.0
35	15306.0
36	15739.5
37	16173.0
38	17853.5
39	19534.0
40	22113.0
41	24692.0
42	27664.0
43	30636.0
44	34382.5
45	38129.0
46	67224.5
47	96320.0
48	77588.5
49	58857.0
50	57813.5
51	56770.0
52	49019.0
53	41268.0
54	36955.0
55	32642.0
56	29710.0
57	26778.0
58	24089.5
59	21401.0
60	19857.5
61	18314.0
62	16459.5
63	14605.0
64	12516.0
65	10427.0
66	8725.0
67	7023.0
68	5861.5
69	4700.0
70	3812.0
71	2924.0
72	2390.0
73	1856.0
74	1467.5
75	915.0
76	751.0
77	543.0
78	335.0
79	236.0
80	137.0
81	112.0
82	87.0
83	56.5
84	26.0
85	15.5
86	5.0
87	4.0
88	3.0
89	2.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	625215.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.116229979053465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.89992322078243	24.376193895511484
2	9.398187167309828	6.036686809030229
3	3.345898334447042	3.223729211868994
4	1.6472934620058408	2.1161942267508302
5	1.0521087637138076	1.6894883509205132
6	0.8002911589728706	1.542140094706557
7	0.6202215835487479	1.3943425310657016
8	0.5135388627870343	1.319434577635998
9	0.4674636005331592	1.3511851651403388
>10	6.09163056696788	39.94503779864308
>50	0.10845773138967559	2.3138523577550703
>100	0.044392001685057164	2.6023953523741032
>500	0.004035636516823379	0.9101822255890999
>1k	0.004540091081426301	3.5629632440647
>5k	5.044545646029223E-4	1.407882438655977
>10k+	0.001513363693808767	6.208291720287345
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCG	12955	2.072087202002511	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGC	12899	2.063130283182585	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTC	12466	1.9938741073070865	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8690	1.3899218668777942	No Hit
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	4726	0.755899970410179	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTACAGGT	3356	0.5367753492798477	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	3015	0.48223411146565587	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	2756	0.44080836192349826	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTA	2513	0.40194173204417677	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTC	2308	0.36915301136409073	No Hit
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	1247	0.19945138872227955	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	1038	0.1660228881264845	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGGTCGAATGC	1033	0.16522316323184824	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACAGGTCGAATGC	847	0.13547339715137993	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTT	818	0.13083499276248972	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC	797	0.12747614820501746	No Hit
TCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	723	0.11564021976440104	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATG	722	0.11548027478547379	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5994497892724902E-4	0.0	0.0	0.2064889677950785	0.0
2	1.5994497892724902E-4	0.0	0.0	1.3688091296593972	0.0
3	1.5994497892724902E-4	0.0	0.0	1.6893388674296042	0.0
4	1.5994497892724902E-4	0.0	0.0	2.535607750933679	0.0
5	1.5994497892724902E-4	0.0	0.0	5.583839159329191	0.0
6	1.5994497892724902E-4	0.0	0.0	6.322784961973081	0.0
7	1.5994497892724902E-4	0.0	0.0	7.421287077245427	0.0
8	1.5994497892724902E-4	0.0	0.0	8.685492190686404	0.0
9	1.5994497892724902E-4	0.0	0.0	9.105027870412577	0.0
10	1.5994497892724902E-4	0.0	0.0	12.147341314587782	0.0
11	1.5994497892724902E-4	0.0	0.0	13.388514351063234	0.0
12	1.5994497892724902E-4	0.0	0.0	16.63827643290708	0.0
13	1.5994497892724902E-4	0.0	0.0	17.223515110801884	0.0
14	1.5994497892724902E-4	0.0	0.0	17.48358564653759	0.0
15	1.5994497892724902E-4	0.0	0.0	18.24700303095735	0.0
16	1.5994497892724902E-4	0.0	0.0	18.963076701614643	0.0
17	1.5994497892724902E-4	0.0	0.0	19.760722311524834	0.0
18	1.5994497892724902E-4	0.0	0.0	20.575801924138098	0.0
19	1.5994497892724902E-4	0.0	0.0	21.670785249874044	0.0
20	1.5994497892724902E-4	0.0	0.0	22.270898810809083	0.0
21	1.5994497892724902E-4	0.0	0.0	22.82462832785522	0.0
22	1.5994497892724902E-4	0.0	0.0	23.470006317826666	0.0
23	1.5994497892724902E-4	0.0	0.0	24.044208792175493	0.0
24	3.1988995785449804E-4	0.0	0.0	24.513647305326966	0.0
25	4.7983493678174706E-4	0.0	0.0	24.9000743744152	0.0
26	4.7983493678174706E-4	0.0	0.0	25.312892365026432	0.0
27	4.7983493678174706E-4	0.0	0.0	25.77929192357829	0.0
28	4.7983493678174706E-4	0.0	0.0	26.15884135857265	0.0
29	4.7983493678174706E-4	0.0	0.0	26.558064025975064	0.0
30	4.7983493678174706E-4	0.0	0.0	27.008469086634197	0.0
31	4.7983493678174706E-4	0.0	0.0	27.4352022904121	0.0
32	4.7983493678174706E-4	0.0	0.0	27.849299840854744	0.0
33	4.7983493678174706E-4	0.0	0.0	28.236206744879762	0.0
34	4.7983493678174706E-4	0.0	0.0	28.617355629663397	0.0
35	4.7983493678174706E-4	0.0	0.0	29.033852354789953	0.0
36	4.7983493678174706E-4	0.0	0.0	29.448909575106164	0.0
37	4.7983493678174706E-4	0.0	0.0	29.845413177866813	0.0
38	4.7983493678174706E-4	0.0	0.0	30.241756835648538	0.0
39	4.7983493678174706E-4	0.0	0.0	30.649296641955168	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATC	20	7.030815E-4	45.000004	19
TCGCACC	20	7.030815E-4	45.000004	32
CGCATCC	20	7.030815E-4	45.000004	20
GTTACGT	20	7.030815E-4	45.000004	7
GTTCGAC	20	7.030815E-4	45.000004	9
CGCTATA	20	7.030815E-4	45.000004	43
CGGTCGA	195	0.0	45.000004	41
GTACGAG	20	7.030815E-4	45.000004	1
CCGATCG	45	3.8380676E-10	45.0	40
CCGTCGA	25	3.888757E-5	45.0	41
TCGGCCA	25	3.888757E-5	45.0	29
CGTAAGC	55	1.8189894E-12	45.0	43
CGGTCTA	50	2.1827873E-11	45.0	31
CCGCGTA	45	3.8380676E-10	45.0	40
CGATCGA	45	3.8380676E-10	45.0	41
TACCGCC	25	3.888757E-5	45.0	45
GTCGAAT	365	0.0	44.383564	43
GGTCGAA	355	0.0	43.09859	42
TAATACG	110	0.0	42.954544	4
CGATGAA	2050	0.0	42.914635	19
>>END_MODULE