##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551251_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544724
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56554879168166	31.0	31.0	34.0	30.0	34.0
2	31.718060889551406	31.0	31.0	34.0	30.0	34.0
3	31.715356767831047	31.0	31.0	34.0	30.0	34.0
4	35.45224003348485	37.0	35.0	37.0	33.0	37.0
5	34.418960794824535	37.0	35.0	37.0	32.0	37.0
6	34.77782142883368	37.0	35.0	37.0	32.0	37.0
7	35.42635536528591	37.0	35.0	37.0	33.0	37.0
8	34.9539748569918	37.0	35.0	37.0	32.0	37.0
9	36.661547866442454	39.0	37.0	39.0	32.0	39.0
10	36.50622333512017	38.0	35.0	39.0	32.0	39.0
11	36.71303999823764	39.0	35.0	39.0	33.0	39.0
12	36.66093104030665	39.0	35.0	39.0	32.0	39.0
13	36.65220184900977	39.0	35.0	39.0	32.0	39.0
14	37.712678347199684	40.0	37.0	41.0	33.0	41.0
15	37.76018130282492	40.0	36.0	41.0	33.0	41.0
16	37.961817360718456	40.0	37.0	41.0	33.0	41.0
17	37.736696014862574	40.0	36.0	41.0	33.0	41.0
18	37.70537373054978	39.0	36.0	41.0	33.0	41.0
19	37.589816127066186	39.0	36.0	41.0	33.0	41.0
20	37.33568375911471	39.0	35.0	41.0	32.0	41.0
21	37.42343278430912	39.0	35.0	41.0	32.0	41.0
22	37.45747938405504	39.0	35.0	41.0	33.0	41.0
23	37.37272086414404	39.0	35.0	41.0	32.0	41.0
24	37.34825893480001	39.0	35.0	41.0	32.0	41.0
25	37.24330303052555	39.0	35.0	41.0	32.0	41.0
26	37.16440435890469	39.0	35.0	41.0	32.0	41.0
27	37.04106850441691	39.0	35.0	41.0	32.0	41.0
28	36.90907872610717	39.0	35.0	41.0	31.0	41.0
29	36.90907321873095	39.0	35.0	41.0	31.0	41.0
30	36.689049867455815	39.0	35.0	41.0	31.0	41.0
31	36.58790506752043	39.0	35.0	41.0	30.0	41.0
32	36.291955191987135	39.0	35.0	41.0	30.0	41.0
33	36.21979754885043	39.0	35.0	41.0	29.0	41.0
34	35.872612552411866	39.0	35.0	41.0	27.0	41.0
35	35.84912542865745	39.0	35.0	41.0	27.0	41.0
36	35.63862249506172	39.0	35.0	41.0	25.0	41.0
37	35.439940226610176	39.0	35.0	41.0	25.0	41.0
38	35.32991386463603	39.0	35.0	41.0	24.0	41.0
39	35.22466606942231	39.0	35.0	40.0	24.0	41.0
40	35.23737158634464	38.0	35.0	40.0	24.0	41.0
41	35.12698540912462	38.0	35.0	40.0	24.0	41.0
42	35.13901535456488	38.0	35.0	40.0	24.0	41.0
43	34.992967080576584	38.0	35.0	40.0	23.0	41.0
44	35.213774315065976	38.0	35.0	40.0	23.0	41.0
45	35.20444114817779	38.0	35.0	40.0	24.0	41.0
46	35.164123482717855	38.0	35.0	40.0	23.0	41.0
47	34.9477533576637	38.0	35.0	40.0	23.0	41.0
48	35.055811750537885	38.0	35.0	40.0	23.0	41.0
49	35.047350584883354	38.0	35.0	40.0	24.0	41.0
50	34.80814320646786	38.0	35.0	40.0	23.0	41.0
51	33.971815084336285	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	30.0
10	38.0
11	38.0
12	32.0
13	23.0
14	57.0
15	53.0
16	81.0
17	135.0
18	240.0
19	540.0
20	1459.0
21	2932.0
22	4535.0
23	5387.0
24	6287.0
25	7464.0
26	8272.0
27	8311.0
28	8061.0
29	7903.0
30	9023.0
31	11436.0
32	14849.0
33	20301.0
34	34576.0
35	43599.0
36	43449.0
37	64125.0
38	104570.0
39	136858.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.307950448300424	17.29462994103436	19.814988875100052	14.582430735565167
2	31.3162261989558	33.02553219612134	20.365726496354117	15.292515108568743
3	27.916706442161537	32.11130774483959	25.360733141921415	14.611252671077462
4	25.746065897592175	20.77657676180965	36.81644282242016	16.660914518178014
5	31.545883787018745	27.0406664659534	23.302259492880797	18.111190254147054
6	22.684331881833735	39.0113158223247	25.35192133998135	12.952430955860214
7	67.00659416511849	5.670210969224781	22.40492432865084	4.918270537005896
8	66.16268055015017	15.356400672634216	13.035225178255411	5.445693598960207
9	60.983911118291104	7.386676555466622	14.06418663396509	17.56522569227719
10	42.84720335435927	21.412495135151012	20.834587791248413	14.905713719241303
11	34.16886349784478	21.659225589472832	25.848319515938346	18.32359139674404
12	26.594385413530524	18.202061961653975	30.19235429318334	25.011198331632166
13	27.615269384128478	21.529067931649788	33.26877464550855	17.586888038713184
14	19.377336045410154	31.28905647630727	28.31709269281324	21.01651478546934
15	17.003106160183872	22.42144645728846	37.80428253574287	22.771164846784796
16	18.915083601970906	22.294226066778773	30.66378569697682	28.126904634273505
17	18.82604768653483	26.79467032845992	31.24940336757697	23.129878617428275
18	19.84858386999655	22.62852380287999	30.653505261380076	26.869387065743382
19	21.157320037303297	24.012527445091457	28.13149411445062	26.698658403154624
20	27.426182800831246	22.998435905155638	31.707066330839105	17.86831496317401
21	22.895631549188213	29.66107606788025	28.149484876744918	19.29380750618662
22	19.99930239901308	22.07888765686843	33.14614373517598	24.77566620894251
23	23.919636366306605	26.00638121323826	28.870951160587747	21.203031259867384
24	22.23144197795581	25.15420653395114	28.62311923102342	23.991232257069637
25	19.711266623097202	31.24022440722274	27.063430287631903	21.985078682048158
26	19.181273452243705	24.116249697094307	33.012498072418325	23.689978778243663
27	26.17417260851367	25.56725974989169	27.135026178394927	21.123541463199714
28	18.39757381719917	28.298551192897687	32.0476057599812	21.25626922992194
29	21.555870495884154	24.722244659680868	32.36960368920774	21.352281155227235
30	22.37867250203773	29.026993486609733	28.82285340833156	19.77148060302098
31	22.66285311460483	23.448939279341467	28.112952614535068	25.775254991518644
32	28.112218297706725	25.924137728464324	27.538900433981244	18.4247435398477
33	28.01712426843686	22.793928668463295	25.95094029269869	23.238006770401157
34	22.08310997863138	25.248015508771417	29.858974453117543	22.809900059479663
35	28.343895257047606	22.712970238138947	26.722340120868548	22.2207943839449
36	24.48249021522826	29.171470322585378	26.675527423061958	19.6705120391244
37	24.285142567612223	24.23612691932061	31.360652367070298	20.118078145996872
38	24.22474500848136	23.918718470271184	27.13135459425324	24.72518192699422
39	22.93822192523186	25.20212070700024	31.047833398197987	20.81182396956991
40	26.357935394805445	21.7746969107291	28.641844310146052	23.2255233843194
41	22.865157400812155	24.21813615702631	28.26385472275868	24.652851719402854
42	25.26949427600032	23.540178145262555	29.10134306547903	22.08898451325809
43	27.28317459851227	24.420624022440723	25.87016544158142	22.42603593746558
44	23.472070259434137	22.938038346024776	30.477085643371687	23.1128057511694
45	22.04676129562861	21.784977346325846	29.073989763623416	27.094271594422132
46	26.518016463383294	25.537336339136885	27.445825775989306	20.498821421490515
47	20.875893112842466	25.594613051747306	33.499166550399835	20.03032728501039
48	23.152458859899692	23.59249821928169	29.067380912168368	24.18766200865025
49	22.236398616547095	21.213862433085378	33.80188866288249	22.74785028748504
50	23.777913218437227	22.338101497272014	30.13085525880997	23.753130025480797
51	21.547976589979513	22.444393858174045	28.486352721745323	27.521276830101115
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	139.0
1	524.5
2	910.0
3	4820.0
4	8730.0
5	5867.0
6	3004.0
7	2852.5
8	2701.0
9	2598.0
10	2495.0
11	2474.5
12	2454.0
13	2405.5
14	2357.0
15	2191.0
16	2025.0
17	2027.0
18	2029.0
19	2033.0
20	2037.0
21	1984.5
22	1932.0
23	2057.0
24	2182.0
25	2527.0
26	3394.0
27	3916.0
28	4130.5
29	4345.0
30	5554.5
31	6764.0
32	7906.0
33	9048.0
34	10138.5
35	11229.0
36	12299.5
37	13370.0
38	14289.5
39	15209.0
40	17678.0
41	20147.0
42	22219.0
43	24291.0
44	27363.0
45	30435.0
46	52132.5
47	73830.0
48	60647.0
49	47464.0
50	47157.5
51	46851.0
52	42085.5
53	37320.0
54	34318.0
55	31316.0
56	29835.5
57	28355.0
58	25982.5
59	23610.0
60	22499.5
61	21389.0
62	20241.0
63	19093.0
64	16503.5
65	13914.0
66	11632.5
67	9351.0
68	8078.5
69	6806.0
70	5560.0
71	4314.0
72	3693.0
73	3072.0
74	2327.5
75	1265.0
76	947.0
77	721.0
78	495.0
79	349.5
80	204.0
81	156.5
82	109.0
83	68.0
84	27.0
85	31.0
86	35.0
87	26.0
88	17.0
89	9.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	544724.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.007355879028463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25202952476494	22.88121786447807
2	8.500273312029222	5.101414526861377
3	2.828347599247448	2.5461369888064187
4	1.5119922005865223	1.8148355219726064
5	1.0367324877961088	1.5554800356324183
6	0.7579893975206315	1.364715456235918
7	0.6567855299013085	1.3795877992361396
8	0.5467350106119678	1.3124857627966178
9	0.48610778354868406	1.312812833085999
>10	7.2422401225644	46.34033384008334
>50	0.12277351281343861	2.3898571114029923
>100	0.048739233729958045	2.8010332400284508
>500	0.0024678093027826857	0.5329923792493
>1k	0.0043186662798697	2.5262987173448934
>5k	0.0024678093027826857	6.14079792278544
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	9676	1.7763124077514485	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	9541	1.7515292147950154	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	8930	1.6393623192662705	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5023	0.9221183571863917	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	3438	0.63114531395716	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTGTGGT	2889	0.5303603292676659	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCT	1946	0.3572451369868043	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	1486	0.2727987017278475	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTA	1421	0.2608660532673427	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTC	1236	0.22690389995667531	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1230	0.22580242471416717	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCTCCGGTCGAATGC	872	0.1600810685778486	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT	749	0.13750082610643188	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	744	0.1365829300710084	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6715841416937753E-4	0.0	0.0	0.1420903062835491	0.0
2	3.6715841416937753E-4	0.0	0.0	1.159669851153979	0.0
3	3.6715841416937753E-4	0.0	0.0	1.4029123005411914	0.0
4	3.6715841416937753E-4	0.0	0.0	1.9396979020568215	0.0
5	3.6715841416937753E-4	0.0	0.0	4.346421306937091	0.0
6	3.6715841416937753E-4	0.0	0.0	4.973711457545472	0.0
7	3.6715841416937753E-4	0.0	0.0	5.797614938941556	0.0
8	3.6715841416937753E-4	0.0	0.0	6.676225024048876	0.0
9	3.6715841416937753E-4	0.0	0.0	6.972521864283563	0.0
10	3.6715841416937753E-4	0.0	0.0	9.429362392697954	0.0
11	3.6715841416937753E-4	0.0	0.0	10.260241883963255	0.0
12	3.6715841416937753E-4	0.0	0.0	12.826679199007204	0.0
13	3.6715841416937753E-4	0.0	0.0	13.2415682070186	0.0
14	5.507376212540662E-4	0.0	0.0	13.441302384326741	0.0
15	5.507376212540662E-4	0.0	0.0	13.954406268128446	0.0
16	5.507376212540662E-4	0.0	0.0	14.48935607757323	0.0
17	5.507376212540662E-4	0.0	0.0	15.053678560151562	0.0
18	5.507376212540662E-4	0.0	0.0	15.663895844501068	0.0
19	5.507376212540662E-4	0.0	0.0	16.620710671826465	0.0
20	7.343168283387551E-4	0.0	0.0	17.066624565835177	0.0
21	7.343168283387551E-4	0.0	0.0	17.456179643268886	0.0
22	7.343168283387551E-4	0.0	0.0	17.921369354021486	0.0
23	7.343168283387551E-4	0.0	0.0	18.33460614916912	0.0
24	7.343168283387551E-4	0.0	0.0	18.690199073292163	0.0
25	7.343168283387551E-4	0.0	0.0	19.004303096614066	0.0
26	7.343168283387551E-4	0.0	0.0	19.283894229004048	0.0
27	9.178960354234438E-4	0.0	0.0	19.67748804899362	0.0
28	9.178960354234438E-4	0.0	0.0	19.9546926516915	0.0
29	9.178960354234438E-4	0.0	0.0	20.2460328533349	0.0
30	9.178960354234438E-4	0.0	0.0	20.61961653975224	0.0
31	0.0011014752425081325	0.0	0.0	20.93041613734662	0.0
32	0.0011014752425081325	0.0	0.0	21.24158289335517	0.0
33	0.0011014752425081325	0.0	0.0	21.538981208832364	0.0
34	0.0012850544495928214	0.0	0.0	21.836563103516642	0.0
35	0.0012850544495928214	0.0	0.0	22.146444805075596	0.0
36	0.0012850544495928214	0.0	0.0	22.458345877912485	0.0
37	0.0012850544495928214	0.0	0.0	22.757947143874695	0.0
38	0.0012850544495928214	0.0	0.0	23.048002291068503	0.0
39	0.0012850544495928214	0.0	0.0	23.351458720379494	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGAT	45	3.8380676E-10	45.000004	38
CCGCGTA	45	3.8380676E-10	45.000004	40
CCGATCG	40	6.8030204E-9	45.0	40
GCGAACC	40	6.8030204E-9	45.0	33
TCGATAG	25	3.888095E-5	45.0	1
ACGGGCC	25	3.888095E-5	45.0	5
TACGCGG	20	7.0300145E-4	45.0	2
TACGATG	20	7.0300145E-4	45.0	1
CGATCGA	40	6.8030204E-9	45.0	41
TCGAATA	20	7.0300145E-4	45.0	44
GGTCGAA	330	0.0	44.31818	42
CGGTCGA	325	0.0	44.307693	41
GATGAAT	2225	0.0	43.280903	20
GTCGAAT	335	0.0	42.985077	43
ATGAATG	2215	0.0	42.9684	21
CGATGAA	2130	0.0	42.887325	19
CCGATGA	2095	0.0	42.74463	18
CGTTTTT	2465	0.0	42.5355	1
ACAACGA	75	0.0	42.0	13
CGTAAGC	75	0.0	42.0	43
>>END_MODULE