FastQCFastQC Report
Sat 18 Jun 2016
SRR3551248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629634
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65791.0448927472150487No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC51180.8128531813720351No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG44980.7143832766337269No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC41980.6667365485345454No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC37100.589231204159877TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC23080.36656216150970244TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT20310.3225683492314583TruSeq Adapter, Index 22 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT15070.23934539748488803No Hit
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC12360.1963045197686275TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAAGGTCCT11980.19026926754273119No Hit
GAATGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT11410.1812163892038867No Hit
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG11290.17931052007991943TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCC10860.17248115571903677No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCT10080.16009300641324958No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8270.13134614712674347No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTC8130.12912263314878167No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTA7440.11816388568596993No Hit
ACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC7390.11736977355098357TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT7160.11371685773004636No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC6970.1106992316170982No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA207.0308545E-445.044
GTTTCGG351.2107557E-745.02
CGGCCCA253.8887894E-545.06
GTTAGCG351.2107557E-745.01
ATTCGCG253.8887894E-545.012
ATTCGAT207.0308545E-445.014
CGTTACG207.0308545E-445.024
CCCGTAT207.0308545E-445.042
TCTCGCG253.8887894E-545.032
ACGTACG207.0308545E-445.01
TTCGCGT253.8887894E-545.013
TTCGAAG207.0308545E-445.01
TAATGCG253.8887894E-545.01
TAGTACG207.0308545E-445.01
GTACGAG207.0308545E-445.01
TCGTTAG302.1639316E-644.9999961
TTAGCGT302.1639316E-644.9999961
CTAACGG302.1639316E-644.9999962
TACGTAG650.044.9999961
CGTTTTT33500.043.5895541
Produced by FastQC (version 0.11.5)